Cloning, Expression in Escherichia coli, and Characterization of
... with or without D-glucose, incubatcd for 4 days at 37°C, and then incubated at 37°C with the appropriate overlay to screen for cellulase activities. Overlays consisted of 8 ml of 0.05 M potassium phosphate (pH 7.0)-buffered agarose (0.7%) containing 0.5 mg of either 4-methylumbelliferyl-3-D-glucosid ...
... with or without D-glucose, incubatcd for 4 days at 37°C, and then incubated at 37°C with the appropriate overlay to screen for cellulase activities. Overlays consisted of 8 ml of 0.05 M potassium phosphate (pH 7.0)-buffered agarose (0.7%) containing 0.5 mg of either 4-methylumbelliferyl-3-D-glucosid ...
Model Answers For Biology B1
... has not happened. Dolly did not kill and eat a lamb – this story, like many in the tabloid press, was made up by journalists. Human cloning is illegal and is not carried out (except in nature in the form of identical twins). Discuss the social and ethical issues around animal cloning. (3 marks) She ...
... has not happened. Dolly did not kill and eat a lamb – this story, like many in the tabloid press, was made up by journalists. Human cloning is illegal and is not carried out (except in nature in the form of identical twins). Discuss the social and ethical issues around animal cloning. (3 marks) She ...
Transport of bacteria on sloping soil surfaces by runoff
... ABSTRACT: Pathogenic bacteria exist at soil surfaces as a result of practices as spreading of liquid manure on agricultural lands or use of treated wastewater for irrigation. Rainfall is a major factor affecting vertical and horizontal movement of bacteria in soil. Surface runoff carries bacteria si ...
... ABSTRACT: Pathogenic bacteria exist at soil surfaces as a result of practices as spreading of liquid manure on agricultural lands or use of treated wastewater for irrigation. Rainfall is a major factor affecting vertical and horizontal movement of bacteria in soil. Surface runoff carries bacteria si ...
Integrin modulation of signaling to transcription factors
... Cells decide whether to undergo processes, such as proliferation, differentiation and apoptosis, based upon the cues they receive from both circulating factors and integrin-mediated adhesion to the extracellular matrix. Integrins control the activation of the early signaling pathways. For example, g ...
... Cells decide whether to undergo processes, such as proliferation, differentiation and apoptosis, based upon the cues they receive from both circulating factors and integrin-mediated adhesion to the extracellular matrix. Integrins control the activation of the early signaling pathways. For example, g ...
PDF - Eleanor Maine Research Lab
... The C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cyc ...
... The C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cyc ...
PDF
... 2009). The pluripotent epiblast cells in the ICM will give rise to all somatic lineages of the embryo and the germline. Notably, this pluripotent capacity persists only transiently for a few days in vivo; however, when explanted in vitro, further development is halted and pluripotency can be capture ...
... 2009). The pluripotent epiblast cells in the ICM will give rise to all somatic lineages of the embryo and the germline. Notably, this pluripotent capacity persists only transiently for a few days in vivo; however, when explanted in vitro, further development is halted and pluripotency can be capture ...
Changes in Red Blood Cell Electrolytes and ATP in
... accumulation. However. in this studv. ATP content of the newborn erythrocyte was unchanged, excluding high energy deficits as the primary factor for cellular dysfunction in shock. It is likely that the tendency for cellular ATP to increase in newborn shock reflects decreased energy use in a low flow ...
... accumulation. However. in this studv. ATP content of the newborn erythrocyte was unchanged, excluding high energy deficits as the primary factor for cellular dysfunction in shock. It is likely that the tendency for cellular ATP to increase in newborn shock reflects decreased energy use in a low flow ...
division plane control in plants: new players in the band
... during cytokinesis had almost no effect on cell plate orientations [44,45]. These studies suggest that the presence of an ADZ during cytokinesis is not critical for phragmoplast guidance, but that the ADZ and/or PPB F-actin plays an important role in the establishment of the cortical division site. ...
... during cytokinesis had almost no effect on cell plate orientations [44,45]. These studies suggest that the presence of an ADZ during cytokinesis is not critical for phragmoplast guidance, but that the ADZ and/or PPB F-actin plays an important role in the establishment of the cortical division site. ...
Synergistic anti-cancer activity of the combination of
... structure of dihydroartemisinin (DHA) ...
... structure of dihydroartemisinin (DHA) ...
Glial cell regulation of neuronal activity and blood flow in the retina
... Figure 2. ATP release from glial cells inhibits retinal ganglion cells (RGCs). (a) An intracellular recording from a RGC that displays spontaneous spiking. Stimulation of glial cells by ATPgS ejection results in prolonged hyperpolarization of the RGC and inhibition of spiking. From Newman [9]. (b) M ...
... Figure 2. ATP release from glial cells inhibits retinal ganglion cells (RGCs). (a) An intracellular recording from a RGC that displays spontaneous spiking. Stimulation of glial cells by ATPgS ejection results in prolonged hyperpolarization of the RGC and inhibition of spiking. From Newman [9]. (b) M ...
SLP-76-Cbl-Grb2-Shc Interactions in Fc RI Signaling
... leukocyte protein of 76 kD) that is found only in hematopoetic cells.36 This 533 amino acid protein contains several tyrosines in its amino-terminal end, a central proline-rich region that ...
... leukocyte protein of 76 kD) that is found only in hematopoetic cells.36 This 533 amino acid protein contains several tyrosines in its amino-terminal end, a central proline-rich region that ...
Cell shape changes during gastrulation in
... been studied in many organisms and various models have been proposed to explain the mechanics of cell and epithelial shape changes, but the molecular mechanisms of the processes are not understood. Molecules that are likely to play important roles in shape changes have been identified and analysed b ...
... been studied in many organisms and various models have been proposed to explain the mechanics of cell and epithelial shape changes, but the molecular mechanisms of the processes are not understood. Molecules that are likely to play important roles in shape changes have been identified and analysed b ...
Characterization of Dependencies Between Growth and
... cell size distribution from generation to generation provided the amount of time they spent in S/G2 /M was similar on average. As such, we tested whether combined S/G2 /M duration is roughly constant and shared across mother and daughter cells [9]. To assess differences in the observed mother and da ...
... cell size distribution from generation to generation provided the amount of time they spent in S/G2 /M was similar on average. As such, we tested whether combined S/G2 /M duration is roughly constant and shared across mother and daughter cells [9]. To assess differences in the observed mother and da ...
Cell Structure and Function
... different pH solutions. One is very acidic and one is very basic. What would happen if you took the biological membrane out of one beaker and put it directly into the other beaker? Your group can’t go to the lab until each member has finished your warmup… get to work, you MUST finish ...
... different pH solutions. One is very acidic and one is very basic. What would happen if you took the biological membrane out of one beaker and put it directly into the other beaker? Your group can’t go to the lab until each member has finished your warmup… get to work, you MUST finish ...
Tetherin Inhibits HIV-1 Release by Directly Tethering Virions to Cells
... maturation, we used a CHO-derived cell line lacking PIGL, an ER-resident enzyme required for the addition of GPI anchors. Proteins that harbor a GPI modification signal as their only membrane anchor are inserted into ER membrane but remain trapped there in the absence of PIGL (Nakamura et al., 1997) ...
... maturation, we used a CHO-derived cell line lacking PIGL, an ER-resident enzyme required for the addition of GPI anchors. Proteins that harbor a GPI modification signal as their only membrane anchor are inserted into ER membrane but remain trapped there in the absence of PIGL (Nakamura et al., 1997) ...
Endocytosis of Viruses and Bacteria
... Many pathogenic bacteria multiply extracellularly, but it is increasingly recognized that many bacteria that were long considered extracellular can also reside, replicate, or persist inside cells (Pizarro-Cerda and Cossart 2006). Depending on the type of host cells ( phagocytic or nonphagocytic) wit ...
... Many pathogenic bacteria multiply extracellularly, but it is increasingly recognized that many bacteria that were long considered extracellular can also reside, replicate, or persist inside cells (Pizarro-Cerda and Cossart 2006). Depending on the type of host cells ( phagocytic or nonphagocytic) wit ...
Review Convergent Extension - Massachusetts Institute of Technology
... Schectman and Holtfreter each showed that dorsal tissues removed from the embryo and cultured in isolation narrowed and elongated just as they would in an intact embryo, demonstrating that the motive force for convergent extension was generated within the tissue, and was not driven by other movement ...
... Schectman and Holtfreter each showed that dorsal tissues removed from the embryo and cultured in isolation narrowed and elongated just as they would in an intact embryo, demonstrating that the motive force for convergent extension was generated within the tissue, and was not driven by other movement ...
Asymmetric Cell Divisions in the Early Embryo of the Leech
... lacked a true coelom (Valentine and Collins 2000). If so, many of the features we associate with modern bilaterian animals may have arisen largely independently within these three lines. On the other hand, others have proposed that the urbilaterian was a segmented eucoelomate with well-developed sen ...
... lacked a true coelom (Valentine and Collins 2000). If so, many of the features we associate with modern bilaterian animals may have arisen largely independently within these three lines. On the other hand, others have proposed that the urbilaterian was a segmented eucoelomate with well-developed sen ...
chromosomes
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor They may have an abnormal cell cycle control system ...
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor They may have an abnormal cell cycle control system ...
PDF
... crab (Table 2). Strong positive reactions for several enzymes were observed for the control Hematodinium-free culture medium which prevented them from being assayed in the Hematodinium cell culture media. Marked increases in enzyme activity between control culture media and Hematodinium cell culture ...
... crab (Table 2). Strong positive reactions for several enzymes were observed for the control Hematodinium-free culture medium which prevented them from being assayed in the Hematodinium cell culture media. Marked increases in enzyme activity between control culture media and Hematodinium cell culture ...
Article
... possible to suggest that EB shows photovoltaic effect in the presence of the Eu2+/Eu3+. An anodic photocurrent has been produced when a potential is applied between zero and the open-circuit voltage. This observation indicates that upon illumination, electrons from the HOMO of EB excite to its LUMO ...
... possible to suggest that EB shows photovoltaic effect in the presence of the Eu2+/Eu3+. An anodic photocurrent has been produced when a potential is applied between zero and the open-circuit voltage. This observation indicates that upon illumination, electrons from the HOMO of EB excite to its LUMO ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.