New Views on the Plant Cytoskeleton
... (1) Are the expression levels of the fusion protein interfering with its normal function? (2) If the fusion protein is being expressed in a heterologous system, is it competing with an endogenous protein for a tubulin- or actin-binding site? (3) Is the positioning of the seedling or explanted organ ...
... (1) Are the expression levels of the fusion protein interfering with its normal function? (2) If the fusion protein is being expressed in a heterologous system, is it competing with an endogenous protein for a tubulin- or actin-binding site? (3) Is the positioning of the seedling or explanted organ ...
Induction and Activation of Plant Secondary Metabolism by External
... plant species. One of the most serious controversies over properties of ATPase is the role of CAM in regulation of the enzyme; inconsistent observations on the CAM-dependence of enzyme activity have been reported from several plants (Askerlund & Evans, 1992). It is not yet clear whether this discrep ...
... plant species. One of the most serious controversies over properties of ATPase is the role of CAM in regulation of the enzyme; inconsistent observations on the CAM-dependence of enzyme activity have been reported from several plants (Askerlund & Evans, 1992). It is not yet clear whether this discrep ...
The Pro-apoptotic Effects of Thalidomide on a Pancreatic Cancer
... years.1) Although it has been demonstrated that gemcitabine is an effective medication for palliation of symptoms and prolonging the survival of patients with advanced pancreatic cancer. Single-agent gemcitabine has shown only a limited benefit, with 1-year survival rates of 20% and a median overall ...
... years.1) Although it has been demonstrated that gemcitabine is an effective medication for palliation of symptoms and prolonging the survival of patients with advanced pancreatic cancer. Single-agent gemcitabine has shown only a limited benefit, with 1-year survival rates of 20% and a median overall ...
Roots, Stems, Leaves Notes PPT
... • Many plants pump sugars into their fruits. • In cold climates, plants pump food into their roots for winter storage. • This stored food must be moved back into the trunk and branches of the plant before growth begins again in the spring. ...
... • Many plants pump sugars into their fruits. • In cold climates, plants pump food into their roots for winter storage. • This stored food must be moved back into the trunk and branches of the plant before growth begins again in the spring. ...
Determination and morphogenesis in the sea urchin embryo
... half (where the jelly canal is located) never undergoes gastrulation, never forms pigment, never forms mesenchyme or its derivatives and instead forms a 'permanent' blastula. The long immobile cilia, characteristic of embryos of this half, do eventually become replaced with shorter beating cilia. Th ...
... half (where the jelly canal is located) never undergoes gastrulation, never forms pigment, never forms mesenchyme or its derivatives and instead forms a 'permanent' blastula. The long immobile cilia, characteristic of embryos of this half, do eventually become replaced with shorter beating cilia. Th ...
CYTOKINESIS IN HIGHER PLANTS
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
CELL MODEL SYSTEMS IN CHARACTERIZING α2
... receptors and their signaling properties, as well as to screen potential (subtypeselective) therapeutic drug candidates or unknown compounds in the search for desired effects. Those kinds of cell systems have conventionally involved the use of established cell lines, transfected to express the recep ...
... receptors and their signaling properties, as well as to screen potential (subtypeselective) therapeutic drug candidates or unknown compounds in the search for desired effects. Those kinds of cell systems have conventionally involved the use of established cell lines, transfected to express the recep ...
Role of Topoisomerase II@3in the Resistance of 9-OH
... experiments, were analyzed. The transfected topoisomerase I!@ cDNA was expressed at a high level in one of them, clone 11. As expected from previous experiments (22), the endogenous topo isomerase ll@ transcript (5.6 kb) was undetectable in the resistant cells (Fig. 2). However, in clone I 1, a topo ...
... experiments, were analyzed. The transfected topoisomerase I!@ cDNA was expressed at a high level in one of them, clone 11. As expected from previous experiments (22), the endogenous topo isomerase ll@ transcript (5.6 kb) was undetectable in the resistant cells (Fig. 2). However, in clone I 1, a topo ...
During Cytokinesis Light Chain, Tctex
... utilized to generate “bait” fusion protein constructs. Bait constructs were generated by PCR from a K46 murine B cell lymphoma cDNA library (described below); these were the N-terminal domains of Fyn (amino acids 1– 85, a mutated version with lysines 7 and 9 mutated to alanines, and truncated versio ...
... utilized to generate “bait” fusion protein constructs. Bait constructs were generated by PCR from a K46 murine B cell lymphoma cDNA library (described below); these were the N-terminal domains of Fyn (amino acids 1– 85, a mutated version with lysines 7 and 9 mutated to alanines, and truncated versio ...
Two Domains of the Human bZIP Transcription Factor
... and alternative poly(A) sites were observed. Several different isoforms caused by alternative splicing were isolated (1), one of them identical to the cDNA sequence of Nrf1 (2). In a later study another isoform was identified without the serine-rich domain, which specifically interacts with the tumo ...
... and alternative poly(A) sites were observed. Several different isoforms caused by alternative splicing were isolated (1), one of them identical to the cDNA sequence of Nrf1 (2). In a later study another isoform was identified without the serine-rich domain, which specifically interacts with the tumo ...
Directional Gravity Sensing in Gravitropism
... well as in chloroplasts in the neighboring cortical cells in Arabidopsis inflorescence stems (48). The chloroplasts in cortical cells occasionally contain small starch granules in their stromal space (Figure 3). The photosynthetic competence of shoot amyloplasts may lead to a functional difference fr ...
... well as in chloroplasts in the neighboring cortical cells in Arabidopsis inflorescence stems (48). The chloroplasts in cortical cells occasionally contain small starch granules in their stromal space (Figure 3). The photosynthetic competence of shoot amyloplasts may lead to a functional difference fr ...
Group-specific effects on coastal bacterioplankton of
... ABSTRACT: Polyunsaturated aldehydes (PUAs), produced as secondary metabolites by diatoms, have been shown to induce toxic effects on a variety of organisms, including copepods and phytoand bacterioplankton. However, the nature of and the players in this interaction remain poorly understood. We teste ...
... ABSTRACT: Polyunsaturated aldehydes (PUAs), produced as secondary metabolites by diatoms, have been shown to induce toxic effects on a variety of organisms, including copepods and phytoand bacterioplankton. However, the nature of and the players in this interaction remain poorly understood. We teste ...
Cytokinesis in Higher Plants
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
Membrane-bound and extracellular P4actamase
... hyphae that forms the substrate mycelium (or vegetative mycelium), which later develops into aerial mycelium. This cell type will form the mature spores by a ...
... hyphae that forms the substrate mycelium (or vegetative mycelium), which later develops into aerial mycelium. This cell type will form the mature spores by a ...
BIOLOGY I MIDTERM OBJECTIVES Chapter 1 (The Nature of
... 7) Explain the difference between hypotonic, hypertonic and isotonic solutions. Describe what will happen to both an animal cell and a plant cell that is placed in these types of solutions (use the words plasmolysis and turgor pressure in reference to plant cells) 8) Explain what turgor pressure is ...
... 7) Explain the difference between hypotonic, hypertonic and isotonic solutions. Describe what will happen to both an animal cell and a plant cell that is placed in these types of solutions (use the words plasmolysis and turgor pressure in reference to plant cells) 8) Explain what turgor pressure is ...
Skeletal Muscle Stem Cells in the Spotlight: The Satellite Cell
... be used to isolate satellite cells on the basis of permanent fluorescent reporter expression in cells derived from myogenic progenitor cells expressing Cre recombinase driven by genes relevant to the myogenic lineage such as Pax3, Myf5, and MyoD [73–75]. When working with such Cre-Lox mouse models t ...
... be used to isolate satellite cells on the basis of permanent fluorescent reporter expression in cells derived from myogenic progenitor cells expressing Cre recombinase driven by genes relevant to the myogenic lineage such as Pax3, Myf5, and MyoD [73–75]. When working with such Cre-Lox mouse models t ...
Hedgehog and Dpp signaling induce cadherin Cad86C expression
... Ectopic expression of Hedgehog also results in apical constriction and apicobasal shortening of cells, typical changes in cell shape that are associated with the normal morphogenetic furrow (Heberlein et al., 1995). Conversely, in the simultaneous absence of Hedgehog and Dpp signal transduction, cel ...
... Ectopic expression of Hedgehog also results in apical constriction and apicobasal shortening of cells, typical changes in cell shape that are associated with the normal morphogenetic furrow (Heberlein et al., 1995). Conversely, in the simultaneous absence of Hedgehog and Dpp signal transduction, cel ...
CYTOKINESIS IN HIGHER PLANTS
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
... tracer FM4-64 makes the latter possibility less likely as both Golgi stacks and cell plate are labeled only 30–60 min after uptake of the tracer (10). The machinery involved in the formation of cytokinetic vesicles has not been identified. However, the following components may be required, by analogy ...
as Adobe PDF - Edinburgh Research Explorer
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
... Dual origin of infundibular cells from anterior floor plate To address whether anterior fp cells contribute to the infundibulum, we performed fate-mapping studies in chick embryos, tracing cells from st 9 until E7 (Fig. 1A-C), when the infundibulum is structurally distinct (Fig. 1D). DiI-labelled ce ...
Franz-Josef Obermair, Aileen Schröter and Michaela Thallmair
... Recently, the generation of autologous pluripotent stem cells from skin fibroblasts was reported (109), thus avoiding the ethical and immunorejection problems. Since these cells were manipulated using viral vectors, a therapeutic application of these converted skin cells must be initialized by thoro ...
... Recently, the generation of autologous pluripotent stem cells from skin fibroblasts was reported (109), thus avoiding the ethical and immunorejection problems. Since these cells were manipulated using viral vectors, a therapeutic application of these converted skin cells must be initialized by thoro ...
FGF-dependent midline-derived progenitor cells in hypothalamic
... Fig. 2. Fgf10 and Fgf3/SOX3 expression in the forming infundibulum and collar zone. (A-C) Anterior fp subpopulations (A and P) used in targeted fate mapping (A) were explanted and cultured in vitro (B) for 30 hours (E2.5 equivalent). The anterior (prosencephalic neck)-derived population expresses Fg ...
... Fig. 2. Fgf10 and Fgf3/SOX3 expression in the forming infundibulum and collar zone. (A-C) Anterior fp subpopulations (A and P) used in targeted fate mapping (A) were explanted and cultured in vitro (B) for 30 hours (E2.5 equivalent). The anterior (prosencephalic neck)-derived population expresses Fg ...
Newman and Zahs, J Neurosci., 18:4022-8, 1998.
... 3) in which [C a 21]i in neighboring glial cells also increases. B, Inhibitory modulation in an ON – OFF neuron. Spike frequency decreases when glial [C a 21]i increases. The peristimulus time histograms of cells in A and B are shown in Figure 2, A and B, respectively. C alibration bars are the same ...
... 3) in which [C a 21]i in neighboring glial cells also increases. B, Inhibitory modulation in an ON – OFF neuron. Spike frequency decreases when glial [C a 21]i increases. The peristimulus time histograms of cells in A and B are shown in Figure 2, A and B, respectively. C alibration bars are the same ...
The Plant Cell Wall Integrity Maintenance
... calmodulin-dependent phosphatase activity. One of the primary targets of calcineurin activity is CRZ1, a zinc finger transcription factor whose nuclear localization is dependent on dephosphorylation by calcineurin (Yoshimoto et al. 2002). Expression analysis has shown that CRZ1 regulates the activit ...
... calmodulin-dependent phosphatase activity. One of the primary targets of calcineurin activity is CRZ1, a zinc finger transcription factor whose nuclear localization is dependent on dephosphorylation by calcineurin (Yoshimoto et al. 2002). Expression analysis has shown that CRZ1 regulates the activit ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.