Nestin Is Required for the Proper SelfRenewal of Neural Stem Cells
... demonstrated by Northern blot using a probe against exon 4 (Fig. 1C) and RT-PCR analysis (data not shown). The absence of nestin protein was further confirmed by Western blot using an antibody against a nestin epitope encoded in exon 4 (Fig. 1D) and immunofluorescence staining (Fig. 1E). Intercross be ...
... demonstrated by Northern blot using a probe against exon 4 (Fig. 1C) and RT-PCR analysis (data not shown). The absence of nestin protein was further confirmed by Western blot using an antibody against a nestin epitope encoded in exon 4 (Fig. 1D) and immunofluorescence staining (Fig. 1E). Intercross be ...
Structure and Nanostructure of the Outer Tangential Epidermal Cell
... 105 pathogenic and/or spoilage microorganisms (for instance by blanching or by high power ultrasonic treatment) followed by refrigerated storage or osmotic dehydration (according to the shelf life intended) could be an alternative to freezing and controlled atmosphere preservation methods. However, ...
... 105 pathogenic and/or spoilage microorganisms (for instance by blanching or by high power ultrasonic treatment) followed by refrigerated storage or osmotic dehydration (according to the shelf life intended) could be an alternative to freezing and controlled atmosphere preservation methods. However, ...
Evo-engineering and the Cellular and Molecular Origins of
... rather than real identifiers. These, in the context of stem/progenitor cells should rely on the ...
... rather than real identifiers. These, in the context of stem/progenitor cells should rely on the ...
Blood and Marrow Stem Cell Transplantation
... Autologous Stem Cell Transplantation. This type of transplantation involves the use of a patient’s own stem cells. The stem cells are collected from marrow or blood, then frozen. The thawed cells are returned to the patient after he or she has received intensive chemotherapy and/or radiation therapy ...
... Autologous Stem Cell Transplantation. This type of transplantation involves the use of a patient’s own stem cells. The stem cells are collected from marrow or blood, then frozen. The thawed cells are returned to the patient after he or she has received intensive chemotherapy and/or radiation therapy ...
Cryoelectron Tomography: Implications for Actin Cytoskeleton
... a 200 nm thick specimen, about 130 equally spaced projections are required, for instance, taken at angular increments of 1° in the range between –65° and +65°. Physical constrains prevent covering the full span of angles, and the resulting »missing wedge« of data results in anisotropic resolution wi ...
... a 200 nm thick specimen, about 130 equally spaced projections are required, for instance, taken at angular increments of 1° in the range between –65° and +65°. Physical constrains prevent covering the full span of angles, and the resulting »missing wedge« of data results in anisotropic resolution wi ...
RNA interference screening in Drosophila primary cells for genes
... both the differentiation program of the myogenic pathway and myoblast fusion, illustrating the remarkable conservation of many aspects of myogenesis between flies and vertebrates (Baylies et al., 1998; Chen and Olson, 2004). However, genetic analyses of myofibril assembly have been limited because t ...
... both the differentiation program of the myogenic pathway and myoblast fusion, illustrating the remarkable conservation of many aspects of myogenesis between flies and vertebrates (Baylies et al., 1998; Chen and Olson, 2004). However, genetic analyses of myofibril assembly have been limited because t ...
Distributed Processing of Sensory Information
... P cell with a dorsal receptive field was stimulated extracellularly with a (0.5 set) train of impulses (at 10 Hz) which was always sufficient to evoke local bending. In order to minimize the effect of habituation, an alternating, A-B design was used in which the intemeuron was hyperpolarized (1.3-4. ...
... P cell with a dorsal receptive field was stimulated extracellularly with a (0.5 set) train of impulses (at 10 Hz) which was always sufficient to evoke local bending. In order to minimize the effect of habituation, an alternating, A-B design was used in which the intemeuron was hyperpolarized (1.3-4. ...
ACALYPHA INDICA INDUCED ARTHRITIS IN WISTAR RATS Research Article
... cells play critical roles in the pathogenesis2. Currently, the clinical need for effective treatment of RA remains unmet and more novel drugs are highly demanded. ...
... cells play critical roles in the pathogenesis2. Currently, the clinical need for effective treatment of RA remains unmet and more novel drugs are highly demanded. ...
A role for the Drosophila Bag-of-marbles protein in
... (1:1000) were incubated with 0.5 µg of affinity-purified His-tagged Bam or 0.5 µg of His-tagged fusion protein for another Drosophila ovarian protein for 30 minutes at 24°C before addition of fixed ovaries. The reactions were performed as described above from this point on. We have noted that detect ...
... (1:1000) were incubated with 0.5 µg of affinity-purified His-tagged Bam or 0.5 µg of His-tagged fusion protein for another Drosophila ovarian protein for 30 minutes at 24°C before addition of fixed ovaries. The reactions were performed as described above from this point on. We have noted that detect ...
Mitochondria Know No Boundaries: Mechanisms and Functions of
... and mtDNA involves mitochondrial fission, facilitated by the interaction of mitochondria with cytoskeletal and endoplasmic reticulum elements (Friedman et al., 2011; Lewis et al., 2016). Mitochondrial partitioning during symmetric division can be envisioned as a stochastic process in which mitochond ...
... and mtDNA involves mitochondrial fission, facilitated by the interaction of mitochondria with cytoskeletal and endoplasmic reticulum elements (Friedman et al., 2011; Lewis et al., 2016). Mitochondrial partitioning during symmetric division can be envisioned as a stochastic process in which mitochond ...
Report into the death of Prisoner D 2016
... in the cell and I assumed that he was in the shower. In my experience I have seen prisoners taking showers at unusual times of the night. I proceeded to the watch tower button and pressed it. I looked back into the cell and I still saw steam. I continued with my guard tour and checked other prisone ...
... in the cell and I assumed that he was in the shower. In my experience I have seen prisoners taking showers at unusual times of the night. I proceeded to the watch tower button and pressed it. I looked back into the cell and I still saw steam. I continued with my guard tour and checked other prisone ...
Microreviews in Cell and Molecular Biology
... plays still another role as a modulator of cell-surface relations and cytoskeletal dynamics. The observed increase in ability for the more particles and the quicker growth rate displayed by dynamin B cells on bacterial lawns appears to be directly linked to the development of stronger cell-surface p ...
... plays still another role as a modulator of cell-surface relations and cytoskeletal dynamics. The observed increase in ability for the more particles and the quicker growth rate displayed by dynamin B cells on bacterial lawns appears to be directly linked to the development of stronger cell-surface p ...
The onset of pigment epithelial proliferation after retinal
... parallel to the original monolayer. Their nuclear membranes are more scalloped than those of normal RPE cell nuclei, and the usual apical-basal surface polarity is not evident. The cell surfaces are lined with many short, undifferentiated processes that sometimes interdigitate with similar processes ...
... parallel to the original monolayer. Their nuclear membranes are more scalloped than those of normal RPE cell nuclei, and the usual apical-basal surface polarity is not evident. The cell surfaces are lined with many short, undifferentiated processes that sometimes interdigitate with similar processes ...
Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat
... (CC125), as shown using quantitative RT-PCR (Figure 1C). Cellular protein extracts were probed with an antiserum of PFLP, a photosynthetic type FDX isolated from sweet pepper [36], however, no signal was detected, possibly because of its low affinity to the Chlamydomonas FDXs. Figure 1. Characteriza ...
... (CC125), as shown using quantitative RT-PCR (Figure 1C). Cellular protein extracts were probed with an antiserum of PFLP, a photosynthetic type FDX isolated from sweet pepper [36], however, no signal was detected, possibly because of its low affinity to the Chlamydomonas FDXs. Figure 1. Characteriza ...
Understanding How Lipopolysaccharide Impacts CD4 T Cell Immunity
... can bias immune responses towards Th1. Culturing human T cells in LPS-free medium for 6 days, followed by non-specific stimulation with PMA plus ionomycin resulted in their production of both IFN-γ and IL-4.56 However, when the T cells were first cultured with Brucella abortus LPS and then stimulate ...
... can bias immune responses towards Th1. Culturing human T cells in LPS-free medium for 6 days, followed by non-specific stimulation with PMA plus ionomycin resulted in their production of both IFN-γ and IL-4.56 However, when the T cells were first cultured with Brucella abortus LPS and then stimulate ...
Document
... abundant on earth (after cellulose) Bond hemicellulose: solidify & protect cell wall (nature’s cement): very difficult to digest Monomers are made in cytoplasm & secreted ...
... abundant on earth (after cellulose) Bond hemicellulose: solidify & protect cell wall (nature’s cement): very difficult to digest Monomers are made in cytoplasm & secreted ...
REVIEWS - Brandeis Life Sciences
... (1985–1995) R. Keller and co-workers use the socalled ‘Keller sandwiches’ to study the morphogenetic movements of gastrulation. T. Doniach, J. Gerhart and C. Phillips used the same model to study planar ...
... (1985–1995) R. Keller and co-workers use the socalled ‘Keller sandwiches’ to study the morphogenetic movements of gastrulation. T. Doniach, J. Gerhart and C. Phillips used the same model to study planar ...
Polypeptide Composition of Chlamydia trachomatis
... 2/GU, J/UW-36/GCx, K/UW-3 l/GCx, L1/440/LN, L2/434/Bu and L3/404/LN. After receipt the strains were passaged once or twice through egg yolk sacs and stored in a freezing medium of phosphate-buffered glutamate, pH 7.4, with added sucrose (SPG) at -70 "C (Bovarnick et al., 1950). Infecfiuify tirration ...
... 2/GU, J/UW-36/GCx, K/UW-3 l/GCx, L1/440/LN, L2/434/Bu and L3/404/LN. After receipt the strains were passaged once or twice through egg yolk sacs and stored in a freezing medium of phosphate-buffered glutamate, pH 7.4, with added sucrose (SPG) at -70 "C (Bovarnick et al., 1950). Infecfiuify tirration ...
Whole mount in situ hybridization shows Endo 16
... IF). Endo 16 mRNA becomes restricted to the midgut (stomach) region at this stage, and this distribution pattern remains the same in plutei after seven days, which is the latest stage examined to date. Endo 16 expression in exogastrulae Fig. 3 shows Endo 16 expression after the blastula stage in emb ...
... IF). Endo 16 mRNA becomes restricted to the midgut (stomach) region at this stage, and this distribution pattern remains the same in plutei after seven days, which is the latest stage examined to date. Endo 16 expression in exogastrulae Fig. 3 shows Endo 16 expression after the blastula stage in emb ...
YSK1 is activated by the Golgi matrix protein GM130 and plays a
... traffic and signaling via the Golgi apparatus are significant in this context. However, to date it is unknown how this integration between Golgi apparatus–associated signaling molecules and Golgi apparatus function could be achieved. Parallels between the organizational functions of Golgi matrix pro ...
... traffic and signaling via the Golgi apparatus are significant in this context. However, to date it is unknown how this integration between Golgi apparatus–associated signaling molecules and Golgi apparatus function could be achieved. Parallels between the organizational functions of Golgi matrix pro ...
Preptin, another peptide product of the pancreatic
... This study demonstrates that preptin, a 34-amino acid peptide hormone secreted from the pancreatic -cell, is anabolic to bone. In vitro, preptin acts on osteoblast-like cells to stimulate proliferation, differentiation, and survival. However, preptin does not affect either osteoclastogenesis or mat ...
... This study demonstrates that preptin, a 34-amino acid peptide hormone secreted from the pancreatic -cell, is anabolic to bone. In vitro, preptin acts on osteoblast-like cells to stimulate proliferation, differentiation, and survival. However, preptin does not affect either osteoclastogenesis or mat ...
Polo kinase and progression through M phase in Drosophila
... The first realization that Polo kinase was highly conserved came when Clay et al. (1993) serendipitously found a mouse homologue and when the budding yeast CDC5 gene was also identified as a homologous protein kinase (Kitada et al., 1993). The cdc5 mutant had been identified in a part of Hartwell’s sem ...
... The first realization that Polo kinase was highly conserved came when Clay et al. (1993) serendipitously found a mouse homologue and when the budding yeast CDC5 gene was also identified as a homologous protein kinase (Kitada et al., 1993). The cdc5 mutant had been identified in a part of Hartwell’s sem ...
Paper 2
... morphologies in a hypha of P. tinctorius grown and examined under the standard conditions described in Materials and Methods. Note that the diagram is not to scale, e.g., the tip cell is not longer than other cells, but has been drawn such to allow for inclusion of more detail. ...
... morphologies in a hypha of P. tinctorius grown and examined under the standard conditions described in Materials and Methods. Note that the diagram is not to scale, e.g., the tip cell is not longer than other cells, but has been drawn such to allow for inclusion of more detail. ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.