Proteolysis and orientation in Dictyostelium slugs
... or more, and then tested by being placed close to a migrating slug, the charcoal will have no effect on the orientation of the slug at all (Fig. 1b). In other words, if the charcoal is saturated with NH, it is no longer capable of attracting the slug; it can no longer remove the NH, in the atmospher ...
... or more, and then tested by being placed close to a migrating slug, the charcoal will have no effect on the orientation of the slug at all (Fig. 1b). In other words, if the charcoal is saturated with NH, it is no longer capable of attracting the slug; it can no longer remove the NH, in the atmospher ...
Diffusion, Osmosis, and Movement across a Membrane
... Some transport proteins are channel proteins, which are tubes through the membrane that are always open or only open when a particular molecule binds to them. The latter type of channel are called ligand-gated ion channels, where the ligand is the molecule that must bind to the protein to open the “ ...
... Some transport proteins are channel proteins, which are tubes through the membrane that are always open or only open when a particular molecule binds to them. The latter type of channel are called ligand-gated ion channels, where the ligand is the molecule that must bind to the protein to open the “ ...
PDF
... homologous cells in synchronous embryos fixed at successive time points were classified as having been in two different phases, there is a 30min period of uncertainty regarding the actual transition from one phase to the next. (2) Whereas cells were judged to have been in G2 or M phase on the basis ...
... homologous cells in synchronous embryos fixed at successive time points were classified as having been in two different phases, there is a 30min period of uncertainty regarding the actual transition from one phase to the next. (2) Whereas cells were judged to have been in G2 or M phase on the basis ...
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized
... Although the content was extremely low, AtMTP1 was clearly detected in T87 cells. The AtMTP1 level varied with the medium. The AtMTP1 level in ‘Deep’ cells cultured in MS medium containing 30 µM Zn was three times greater than that of the cells in the JPL medium. Then we examined the effect of the Z ...
... Although the content was extremely low, AtMTP1 was clearly detected in T87 cells. The AtMTP1 level varied with the medium. The AtMTP1 level in ‘Deep’ cells cultured in MS medium containing 30 µM Zn was three times greater than that of the cells in the JPL medium. Then we examined the effect of the Z ...
Quantitative Receptor Binding Assay of Interleukin
... IL-l activity or IL-l receptor expression. Thus, IL-l inhibitors isolated from febrile patient urine were analyzed by this method. IL-l receptor expression on mouse thymocytes along with expressions of other surface markers was also studied. Interleukin-l (IL-l) has a broad spectrum of biological ac ...
... IL-l activity or IL-l receptor expression. Thus, IL-l inhibitors isolated from febrile patient urine were analyzed by this method. IL-l receptor expression on mouse thymocytes along with expressions of other surface markers was also studied. Interleukin-l (IL-l) has a broad spectrum of biological ac ...
Cyclin synthesis and degradation and the embryonic cell cycle
... conventionally assayed by its ability to induce maturation in frog oocytes and has been detected by this assay in extracts from cells in either mitosis or meiosis from a wide variety of eukaryotes, demonstrating both its ubiquity and its functional conservation during evolution (Kishimoto and Kanata ...
... conventionally assayed by its ability to induce maturation in frog oocytes and has been detected by this assay in extracts from cells in either mitosis or meiosis from a wide variety of eukaryotes, demonstrating both its ubiquity and its functional conservation during evolution (Kishimoto and Kanata ...
Lecture 19. secondary phloem-2
... • Secondary Phloem is produced by the Vascular Cambium and can form form a continuous cylinder of tissue opposite secondary xylem in woody plants. • It is a complex tissue which contains Sieve Elements. – Sieve Cells (SC) are characteristic for Gymnosperms, – while Angiosperms produce Sieve Tube Mem ...
... • Secondary Phloem is produced by the Vascular Cambium and can form form a continuous cylinder of tissue opposite secondary xylem in woody plants. • It is a complex tissue which contains Sieve Elements. – Sieve Cells (SC) are characteristic for Gymnosperms, – while Angiosperms produce Sieve Tube Mem ...
Phenotypic Mixing of Retroviruses in Mitogen
... higher than those observed in BALB/c cultures (Table 1). Thus it appears that NFS/N cells also produce a defective retrovirus which can be distinguished from the 129 virus (Jongstra & Moroni, 198 l) by the presence of reverse transcriptase. BUdR treatment appeared to inhibit the production of this v ...
... higher than those observed in BALB/c cultures (Table 1). Thus it appears that NFS/N cells also produce a defective retrovirus which can be distinguished from the 129 virus (Jongstra & Moroni, 198 l) by the presence of reverse transcriptase. BUdR treatment appeared to inhibit the production of this v ...
Variable morphology of human immunodeficiency virus-associated
... immunoblastic morphotype in the context of acquired immunodeficiency syndrome (AIDS), reflecting the morphologic evolution of Burkii lymphoma cells observed in culture. 0 1993 by The American Society of Hematology. ...
... immunoblastic morphotype in the context of acquired immunodeficiency syndrome (AIDS), reflecting the morphologic evolution of Burkii lymphoma cells observed in culture. 0 1993 by The American Society of Hematology. ...
Laminar Shear Stress Inhibits Endothelial Cell Metabolism via KLF2
... 2-N-7-nitrobenz-2-oxa-1,3-diazol-4-yl-amino-2-deoxyglucose (2-NBD)-glucose uptake (bottom) in HUVECs on KLF2 lentiviral transduction compared with mock-transduced control measured by flow cytometry analysis of 2-NBD-glucose fluorescence (n=6). C, Graphical representation of glucose transwell setup ( ...
... 2-N-7-nitrobenz-2-oxa-1,3-diazol-4-yl-amino-2-deoxyglucose (2-NBD)-glucose uptake (bottom) in HUVECs on KLF2 lentiviral transduction compared with mock-transduced control measured by flow cytometry analysis of 2-NBD-glucose fluorescence (n=6). C, Graphical representation of glucose transwell setup ( ...
The a4 Nicotinic Receptor Promotes CD4 T
... (Kawashima and Fujii, 2003). In particular, a significant number of T-lymphocyte markers were observed in the a41 fraction, including the helper T-cell marker glycoproteins CD4, CD28, CCR4, and CXCR3. Expression of CD4 was confirmed in a41 cells derived from the bead assay using immunoblotting (Fig. ...
... (Kawashima and Fujii, 2003). In particular, a significant number of T-lymphocyte markers were observed in the a41 fraction, including the helper T-cell marker glycoproteins CD4, CD28, CCR4, and CXCR3. Expression of CD4 was confirmed in a41 cells derived from the bead assay using immunoblotting (Fig. ...
ASEXUAL REPRODUCTION a result of mitosis
... Advantages of asexual reproduction • One advantage of asexual reproduction is that it allows organisms to reproduce without a mate, saving time and energy. • Asexual reproduction also enables some organisms to produce large numbers of offspring in a short period of time. ...
... Advantages of asexual reproduction • One advantage of asexual reproduction is that it allows organisms to reproduce without a mate, saving time and energy. • Asexual reproduction also enables some organisms to produce large numbers of offspring in a short period of time. ...
Acute Inflammation
... is a basic component of cell migration and recognition and underlies many biologic processes, including embryogenesis, tissue repair, and immune and inflammatory responses. It is, therefore, not surprising that many different genes have evolved that encode proteins with specific adhesive functions. ...
... is a basic component of cell migration and recognition and underlies many biologic processes, including embryogenesis, tissue repair, and immune and inflammatory responses. It is, therefore, not surprising that many different genes have evolved that encode proteins with specific adhesive functions. ...
Functional expression of P2 receptors in the inner ear of chicken
... the correct synapses at periphery and central level in the vestibular system. Consistent with this, our results in E21 showed minimal changes, probably because the synaptic contacts are established and the ATP role in this context may not be needed. Similar findings were observed in the auditory syst ...
... the correct synapses at periphery and central level in the vestibular system. Consistent with this, our results in E21 showed minimal changes, probably because the synaptic contacts are established and the ATP role in this context may not be needed. Similar findings were observed in the auditory syst ...
Rabbit (polyclonal) Anti-Mouse BID Cleavage Site (59/60) Specific
... 8. Assemble the gel and membrane into the sandwich apparatus. 9. Transfer the proteins at 140 mA for 60-90 minutes at room temperature. 10. Following the transfer, rinse the membrane with Tris buffered saline for 2 minutes. 11. Block the membrane with blocking buffer (formulation provided below) for ...
... 8. Assemble the gel and membrane into the sandwich apparatus. 9. Transfer the proteins at 140 mA for 60-90 minutes at room temperature. 10. Following the transfer, rinse the membrane with Tris buffered saline for 2 minutes. 11. Block the membrane with blocking buffer (formulation provided below) for ...
Defining the three cell lineages of the human blastocyst by single
... 2010; Niakan et al., 2012). Mouse embryos also undergo zygotic/ embryo genome activation immediately after fertilization (Flach et al., 1982), whereas it remains unclear whether this occurs between the 4- and 8-cell stage or earlier in human embryos. Three cell lineages comprise the blastocyst: plur ...
... 2010; Niakan et al., 2012). Mouse embryos also undergo zygotic/ embryo genome activation immediately after fertilization (Flach et al., 1982), whereas it remains unclear whether this occurs between the 4- and 8-cell stage or earlier in human embryos. Three cell lineages comprise the blastocyst: plur ...
technicolour transgenics: imaging tools for functional
... without the loss of characteristic properties, such as fluorescence. QUANTUM YIELD ...
... without the loss of characteristic properties, such as fluorescence. QUANTUM YIELD ...
Sigma Xi, The Scientific Research Society
... important component of amino acids, proteins, and many other constituents of plant cells and tissues. Trace elements serve largely to facilitate thework of enzymes, and the fact that they are essential for growth can be demonstrated only with themost meticulous techniques ...
... important component of amino acids, proteins, and many other constituents of plant cells and tissues. Trace elements serve largely to facilitate thework of enzymes, and the fact that they are essential for growth can be demonstrated only with themost meticulous techniques ...
Quantification of Multiple Gene Expression in Individual Cells
... Quantitative gene expression analysis aims to define the gene expression patterns determining cell behavior. So far, these assessments can only be performed at the population level. Therefore, they determine the average gene expression within a population, overlooking possible cell-to-cell heterogen ...
... Quantitative gene expression analysis aims to define the gene expression patterns determining cell behavior. So far, these assessments can only be performed at the population level. Therefore, they determine the average gene expression within a population, overlooking possible cell-to-cell heterogen ...
Assignments Handbook - Independence High
... What is phenylketonuria? Phenylketonuria (commonly known as PKU) is an inherited disorder that increases the levels of a substance called phenylalanine in the blood. Phenylalanine (an amino acid) is a building block of proteins that is obtained through the diet. It is found in all proteins and in so ...
... What is phenylketonuria? Phenylketonuria (commonly known as PKU) is an inherited disorder that increases the levels of a substance called phenylalanine in the blood. Phenylalanine (an amino acid) is a building block of proteins that is obtained through the diet. It is found in all proteins and in so ...
An in vitro System to Study Interactions between Bacteria and
... Sellwood et al., 1975) and other animals (Mooseker, 1976)has been described. Some of these procedures (Forstner et al., 1968; Sellwood et al., 1975) were unsatisfactory, since they yielded fragmented brush borders or failed to separate brush borders from the underlying cellular material (Mooseker, 1 ...
... Sellwood et al., 1975) and other animals (Mooseker, 1976)has been described. Some of these procedures (Forstner et al., 1968; Sellwood et al., 1975) were unsatisfactory, since they yielded fragmented brush borders or failed to separate brush borders from the underlying cellular material (Mooseker, 1 ...
the Adult Drosophila Fat Body
... animals reared at the higher temperature (12 and 24% per unit area of the tissue of 7- and 14-day animals, respectively, at 29°C). Thus the presence of SBMM seems to be directly related to the 1163 gene. However, it is possible that there are other mutations in the stock contributing to other cellul ...
... animals reared at the higher temperature (12 and 24% per unit area of the tissue of 7- and 14-day animals, respectively, at 29°C). Thus the presence of SBMM seems to be directly related to the 1163 gene. However, it is possible that there are other mutations in the stock contributing to other cellul ...
FYVE-dependent endosomal targeting of an arrestin-related
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
Adhesion Molecules: The Path to a New Understanding
... almost all leukocytes (L) and only virtually absent on a subset of memory lymphocytes. Whereas L-selectin expression is restricted to leukocytes, P-selectin is constitutively expressed on platelets (P) and induced, for example, on thrombin- or histamine-activated endothelial cells. E-selectin is exp ...
... almost all leukocytes (L) and only virtually absent on a subset of memory lymphocytes. Whereas L-selectin expression is restricted to leukocytes, P-selectin is constitutively expressed on platelets (P) and induced, for example, on thrombin- or histamine-activated endothelial cells. E-selectin is exp ...
Sample Chapter
... cells, the basic units of an organism. All cells have much in common, yet those in different tissues are distinctive in a number of ways. Cells vary considerably in size. We measure cell sizes in units called micrometers (mi′kro-me″terz). A micrometer equals one thousandth of a millimeter and is sym ...
... cells, the basic units of an organism. All cells have much in common, yet those in different tissues are distinctive in a number of ways. Cells vary considerably in size. We measure cell sizes in units called micrometers (mi′kro-me″terz). A micrometer equals one thousandth of a millimeter and is sym ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.