Increased Functional Half-life of Fibroblast Growth Factor
... variations, three positions are absolutely conserved in the entire human FGF family members including Gly71, Cys83 and Phe132 (based on the 140 amino acid numbering scheme of FGF-1). Cys83 has brought more attention because highly conserved cysteine residues in proteins are often involved in functio ...
... variations, three positions are absolutely conserved in the entire human FGF family members including Gly71, Cys83 and Phe132 (based on the 140 amino acid numbering scheme of FGF-1). Cys83 has brought more attention because highly conserved cysteine residues in proteins are often involved in functio ...
Global Proteomics of the Extremophile Black Fungus Cryomyces
... carotenoids in colourless and brown-red formations; and 3) the dense cell wall built of chitin, melanin and polysaccharides [9]. All those characteristics make the fungi able to survive diverse stress factors [10]-[12]; they are even known to be survivals of outer space and Mars like environments [1 ...
... carotenoids in colourless and brown-red formations; and 3) the dense cell wall built of chitin, melanin and polysaccharides [9]. All those characteristics make the fungi able to survive diverse stress factors [10]-[12]; they are even known to be survivals of outer space and Mars like environments [1 ...
Essential Cell Biology (3rd ed.)
... When proteins fold incorrectly, they sometimes form aggregates that can damage cells and even whole tissues. Aggregated proteins underlie a number of neurodegenerative disorders, including Alzheimer’s disease and Huntington’s disease. Prion diseases—such as scrapie in sheep, bovine spongiform enceph ...
... When proteins fold incorrectly, they sometimes form aggregates that can damage cells and even whole tissues. Aggregated proteins underlie a number of neurodegenerative disorders, including Alzheimer’s disease and Huntington’s disease. Prion diseases—such as scrapie in sheep, bovine spongiform enceph ...
what we`re reading
... Pros: As with most plant-based proteins, pea protein is hypoallergenic. And with few additives or artificial ingredients, this one appeals to those looking for protein sources closest to the whole-food source. Don’t like peas? Don’t worry, the protein version doesn’t taste like mush! Cons: Isolated ...
... Pros: As with most plant-based proteins, pea protein is hypoallergenic. And with few additives or artificial ingredients, this one appeals to those looking for protein sources closest to the whole-food source. Don’t like peas? Don’t worry, the protein version doesn’t taste like mush! Cons: Isolated ...
X(0,25) - IFSC-USP
... Gly swings both ways. The vast majority of all known folds have three or more Gly residues that turn in a way that only they can.These Glys are retained in all members of a fold family(ie. all Bacterial ribosomal proteins). Pro has a constrained conformation. While Pros can be replaced by other amin ...
... Gly swings both ways. The vast majority of all known folds have three or more Gly residues that turn in a way that only they can.These Glys are retained in all members of a fold family(ie. all Bacterial ribosomal proteins). Pro has a constrained conformation. While Pros can be replaced by other amin ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
... judiciously using RPAA, it should be theoretically possible to balance the amino acid needs of the cow while reducing crude protein intake. Broderick and his colleagues (2008) published a study that demonstrated that a ration with 16.1% CP and added RPMet resulted in the same amount of milk as a 17. ...
... judiciously using RPAA, it should be theoretically possible to balance the amino acid needs of the cow while reducing crude protein intake. Broderick and his colleagues (2008) published a study that demonstrated that a ration with 16.1% CP and added RPMet resulted in the same amount of milk as a 17. ...
Challenges in characterising biopharmaceuticals for ease of
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
... The last route assessed by Swanson (1977) was MFP, for which it is not easy to have a clear description. For example, the definition of MFP has changed with time, from “a residue of body secretions and tissue incident to movement of food through the gastrointestinal tract” (Swanson, 1977) to the de ...
... The last route assessed by Swanson (1977) was MFP, for which it is not easy to have a clear description. For example, the definition of MFP has changed with time, from “a residue of body secretions and tissue incident to movement of food through the gastrointestinal tract” (Swanson, 1977) to the de ...
Nutrition for Swimmers
... • Simple carbs can also be referred to as quick burning carbs because they are digested very quickly in the body • These types of carbs cause a big spike in blood sugar • Complex carbs can also be called slow burning carbs because they provide a sustained output of energy over a longer period of tim ...
... • Simple carbs can also be referred to as quick burning carbs because they are digested very quickly in the body • These types of carbs cause a big spike in blood sugar • Complex carbs can also be called slow burning carbs because they provide a sustained output of energy over a longer period of tim ...
Integrative Assignment - California State University
... Because it is so difficult to get enough absolutely pure Complex IV from human tissue to crystalize… this is formally all we can say about the structure of Cytochrome c Oxidase from human mitochondria. However, it is less challenging to obtain a large amount of beef heart mitochondria from slaughte ...
... Because it is so difficult to get enough absolutely pure Complex IV from human tissue to crystalize… this is formally all we can say about the structure of Cytochrome c Oxidase from human mitochondria. However, it is less challenging to obtain a large amount of beef heart mitochondria from slaughte ...
Structural insights into the first incision reaction
... Lin and Sancar, 1992a, b; Verhoeven et al, 2000). UvrD (helicase II) and DNA polymerase I (polI) are required for turnover of the UvrABC proteins (Caron et al, 1985; Husain et al, 1985). UvrD removes both UvrC and the oligonucleotide containing the lesion, while UvrB remains bound to the gapped DNA ...
... Lin and Sancar, 1992a, b; Verhoeven et al, 2000). UvrD (helicase II) and DNA polymerase I (polI) are required for turnover of the UvrABC proteins (Caron et al, 1985; Husain et al, 1985). UvrD removes both UvrC and the oligonucleotide containing the lesion, while UvrB remains bound to the gapped DNA ...
Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete
... adhesion of cells to cellulose in F. succinogenes (11) and R. flavefaciens (18). The genus Caldicellulosiruptor employs a variety of multifunctional enzymes, both cellanchored and “free”, to deconstruct plant biomass at high temperatures (65 to 80°C, 19-21). In fact, one of the notable characteristi ...
... adhesion of cells to cellulose in F. succinogenes (11) and R. flavefaciens (18). The genus Caldicellulosiruptor employs a variety of multifunctional enzymes, both cellanchored and “free”, to deconstruct plant biomass at high temperatures (65 to 80°C, 19-21). In fact, one of the notable characteristi ...
S2 Protocol.
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
Co-evolving residues in membrane proteins
... changes were described by analyzing individual families having a solved structure (Altschuh et al., 1987, 1988). Amino acid contacts were initially thought to be primary spots of these compensatory processes, making the detection of sequence positions with correlated mutational behavior an interesti ...
... changes were described by analyzing individual families having a solved structure (Altschuh et al., 1987, 1988). Amino acid contacts were initially thought to be primary spots of these compensatory processes, making the detection of sequence positions with correlated mutational behavior an interesti ...
Materials and Methods
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
Self Menu Template (Men) - Lyn
... and chicken (animal protein) or bread (grain) and eggs (animal protein). Coconut Milk and animal proteins are a test. In winter we always have either a cooked vegetable or a soup with lunch to aid digestion. Dinner always has cooked vegetables and a raw veg salad as raw vegetables contain enzymes. C ...
... and chicken (animal protein) or bread (grain) and eggs (animal protein). Coconut Milk and animal proteins are a test. In winter we always have either a cooked vegetable or a soup with lunch to aid digestion. Dinner always has cooked vegetables and a raw veg salad as raw vegetables contain enzymes. C ...
Protein Sorting between Mitochondrial Membranes Specified by
... (Fig. 4, lane 4), leaving a large protease-resistant fragment (p' in Fig. 3), which resisted extraction by alkaline treatment (Fig. 3, lane 6). In contrast, pOCT-GST319 located in the inner membrane was resistant to degradation under these conditions (Fig. 2), indicating that the protease was not ga ...
... (Fig. 4, lane 4), leaving a large protease-resistant fragment (p' in Fig. 3), which resisted extraction by alkaline treatment (Fig. 3, lane 6). In contrast, pOCT-GST319 located in the inner membrane was resistant to degradation under these conditions (Fig. 2), indicating that the protease was not ga ...
Sequence ID: ref|WP_006700522.1
... Clone several, using one known to work to find orthologs Use sequence to design primers to clone cDNA ...
... Clone several, using one known to work to find orthologs Use sequence to design primers to clone cDNA ...
Detailed Computational Study of Point Mutations of the TP53 Tumor-Suppressor Protein
... both the molecular and chromosomal level. Inherent in models for malignant progression is the concept that an initial mutation in an important regulatory gene (protein) may be pivotal in this process. Once the initial mutation is introduced, loss of normal gene function or the acquisition of deleter ...
... both the molecular and chromosomal level. Inherent in models for malignant progression is the concept that an initial mutation in an important regulatory gene (protein) may be pivotal in this process. Once the initial mutation is introduced, loss of normal gene function or the acquisition of deleter ...
GroEL and GroES - ETH - D-INFK - TI
... •The operational cycle of the complex GroEL-GroES can best be described through individual points. •When capped by GroES, the GroEL rings have two different states. The first is open, where the ring is available for the reception of misfolded proteins. The second is closed, and actually contains a m ...
... •The operational cycle of the complex GroEL-GroES can best be described through individual points. •When capped by GroES, the GroEL rings have two different states. The first is open, where the ring is available for the reception of misfolded proteins. The second is closed, and actually contains a m ...
THE DETERMINATION OF PROTEIN IN CEREBROSPINAL FLUID
... a method which is insome respects, undoubtedly better than the one just discussed. The technical error is much smaller than that involved in the amino acid method3, the nitrogen content of various proteins is apparently more nearly constant than is their amino acid composition, and the method is mor ...
... a method which is insome respects, undoubtedly better than the one just discussed. The technical error is much smaller than that involved in the amino acid method3, the nitrogen content of various proteins is apparently more nearly constant than is their amino acid composition, and the method is mor ...
A Simple Method for Displaying the Hydropathic Character of a Protein
... in a given amino acid sequence and then successively sums those values, starting at the ammo terminal, within overlapping segments displaced from each other by one residue. Although a segment of any size can be chosen, ordinarily spans of 7, 9, 11 or 13 were employed, odd numbers being used so that ...
... in a given amino acid sequence and then successively sums those values, starting at the ammo terminal, within overlapping segments displaced from each other by one residue. Although a segment of any size can be chosen, ordinarily spans of 7, 9, 11 or 13 were employed, odd numbers being used so that ...
Prediction of mitochondrial proteins of malaria parasite
... The evolutionary information has been used successfully in developing prediction methods for example in prediction of protein secondary structure (Kaur and Raghava 2004a, b), nucleotide-binding proteins (Kumar et al. 2008; Kumar et al. 2007), subcellular localization of proteins. Thus, we also used ...
... The evolutionary information has been used successfully in developing prediction methods for example in prediction of protein secondary structure (Kaur and Raghava 2004a, b), nucleotide-binding proteins (Kumar et al. 2008; Kumar et al. 2007), subcellular localization of proteins. Thus, we also used ...
Modeling in Biology - Server users.dimi.uniud.it
... identification, characterization and classification of the logical and informational modules that operate in cells. For example, the types of modules that may be involved in the dynamics of intracellular communication include feedback loops, switches, timers, oscillators and amplifiers. Many of thes ...
... identification, characterization and classification of the logical and informational modules that operate in cells. For example, the types of modules that may be involved in the dynamics of intracellular communication include feedback loops, switches, timers, oscillators and amplifiers. Many of thes ...
Homology modeling
Homology modeling, also known as comparative modeling of protein, refers to constructing an atomic-resolution model of the ""target"" protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein (the ""template""). Homology modeling relies on the identification of one or more known protein structures likely to resemble the structure of the query sequence, and on the production of an alignment that maps residues in the query sequence to residues in the template sequence. It has been shown that protein structures are more conserved than protein sequences amongst homologues, but sequences falling below a 20% sequence identity can have very different structure.Evolutionarily related proteins have similar sequences and naturally occurring homologous proteins have similar protein structure.It has been shown that three-dimensional protein structure is evolutionarily more conserved than would be expected on the basis of sequence conservation alone.The sequence alignment and template structure are then used to produce a structural model of the target. Because protein structures are more conserved than DNA sequences, detectable levels of sequence similarity usually imply significant structural similarity.The quality of the homology model is dependent on the quality of the sequence alignment and template structure. The approach can be complicated by the presence of alignment gaps (commonly called indels) that indicate a structural region present in the target but not in the template, and by structure gaps in the template that arise from poor resolution in the experimental procedure (usually X-ray crystallography) used to solve the structure. Model quality declines with decreasing sequence identity; a typical model has ~1–2 Å root mean square deviation between the matched Cα atoms at 70% sequence identity but only 2–4 Å agreement at 25% sequence identity. However, the errors are significantly higher in the loop regions, where the amino acid sequences of the target and template proteins may be completely different.Regions of the model that were constructed without a template, usually by loop modeling, are generally much less accurate than the rest of the model. Errors in side chain packing and position also increase with decreasing identity, and variations in these packing configurations have been suggested as a major reason for poor model quality at low identity. Taken together, these various atomic-position errors are significant and impede the use of homology models for purposes that require atomic-resolution data, such as drug design and protein–protein interaction predictions; even the quaternary structure of a protein may be difficult to predict from homology models of its subunit(s). Nevertheless, homology models can be useful in reaching qualitative conclusions about the biochemistry of the query sequence, especially in formulating hypotheses about why certain residues are conserved, which may in turn lead to experiments to test those hypotheses. For example, the spatial arrangement of conserved residues may suggest whether a particular residue is conserved to stabilize the folding, to participate in binding some small molecule, or to foster association with another protein or nucleic acid. Homology modeling can produce high-quality structural models when the target and template are closely related, which has inspired the formation of a structural genomics consortium dedicated to the production of representative experimental structures for all classes of protein folds. The chief inaccuracies in homology modeling, which worsen with lower sequence identity, derive from errors in the initial sequence alignment and from improper template selection. Like other methods of structure prediction, current practice in homology modeling is assessed in a biennial large-scale experiment known as the Critical Assessment of Techniques for Protein Structure Prediction, or CASP.