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Dominant negative connexin26 mutation R75W causing severe
Dominant negative connexin26 mutation R75W causing severe

... labeling. (E-H) Three-dimensional images were constructed with the above confocal images of C-Casp3 and F-actin labeling with (G, H) or without (E, F) nuclear labeling. Bars indicate 20 μm. ...
Research Project Final Report
Research Project Final Report

... The primary aim of this project was to identify cell lines directly permissive to ovine scrapie by further investigation of existing cell lines with proven ability to propagate sheep scrapie, through the development and characterisation of further Rov lines expressing natural ovine PrP alleles and n ...
CYTOCHALASIN B: ASPECTS OF PHAGOCYTOSIS IN NUTRIENT
CYTOCHALASIN B: ASPECTS OF PHAGOCYTOSIS IN NUTRIENT

... We too, have observed that addition of cytochalasin B leads to significantly reduced rates of food vacuole formation, and, in an unsupplemented peptone broth, also of cell multiplication. Similar inhibitions are observed when T. pyriformis is suspended in particle-free, sterile-filtered proteose pep ...
Cells Eukaryotes and Prokaryotes Life - Eukaryotes
Cells Eukaryotes and Prokaryotes Life - Eukaryotes

... not all bacteria are dangerous or disease causing (MH - the adult human in addition bacteria to the skin surface and lining of the respiratory/digestive tract, also has intestines contains trillions of bacteria made up from hundreds of species and thousands of subspecies) biochemically diverse simpl ...
``Biology of Cultured Cells``. In: Culture of Animal Cells, 5th Edition
``Biology of Cultured Cells``. In: Culture of Animal Cells, 5th Edition

... junctional complexes (desmosomes, adherens junctions, and tight junctions) holding them together, whereas mesenchymal cells, which are more dependent on matrix interactions for intercellular bonding, are more easily dissociated. Endothelial cells may also express tight junctions in culture, especial ...
Volume 7, (2002) pp 1137 – 1151 http://www.cmbl.org.pl Received
Volume 7, (2002) pp 1137 – 1151 http://www.cmbl.org.pl Received

... The role of arabinogalactan in the transfer of intercellular signals can be also revealed using the Yariv’s reagent, which binds to arabinogalactan proteins [26]. The addition of this synthetic phenylglucoside to the medium blocks somatic embryogenesis, which was shown for example in suspension cult ...
Apoptosis-inducing factor is involved in the regulation of caspase
Apoptosis-inducing factor is involved in the regulation of caspase

... induces neuronal cell death through a rapid Apaf1-dependent pathway in wild-type neurons, and through a delayed pathway in Apaf1-deficient neurons. These two cell death pathways could be further distinguished on the basis of caspase involvement and cellular morphology. In wild-type neurons, camptoth ...
PDF
PDF

... Taken at face value, Figure 1 shows how the epidermal cell files are tilted in relation to the organ axis while the underlying, radially expanded and shortened cell files do not, but are aligned with the organ axis. This implies that the epidermal cell layer is also tilted with respect to the underlyi ...
Cytokinesis in flowering plants: cellular process
Cytokinesis in flowering plants: cellular process

Self-Replication Mechanism by Means of Self
Self-Replication Mechanism by Means of Self

... Ontogenetic features correspond thus to the way an organism develops from a single cell to an entire body, as well as to the capability of self-repair. Our new platform will therefore logically allow for cellular development and self-repair. The idea behind these two concepts is to let the reconfigu ...
The nucleolar structure and nucleolar proteins as indicators of cell
The nucleolar structure and nucleolar proteins as indicators of cell

... progression, among them ribosome biogenesis, which is morphologically expressed as the nucleolus. The connection is established through nucleolar proteins, which regulate the synthesis and processing of preribosomal precursors and, at the same time, are targets of various cell cycle regulators, such ...
Cumulative cell division time asymmetry in Staphyloccus aureus
Cumulative cell division time asymmetry in Staphyloccus aureus

... In E. coli the development of a microcolony takes place on a single plane for at least a few generations and therefore it is possible to keep a track of the clone for at least 5-6 generations. This enables keeping a track of old and new pole cells and showing that the division time of old pole cells ...
An Adaptive Multiple Access Protocol for Broadcast Channels
An Adaptive Multiple Access Protocol for Broadcast Channels

review - Nature
review - Nature

... Inducible Fas-resistance in B cells [46-48]. In view of the importance of Btk for sIg-induced proliferation, the role of Btk in sIg signaling for Fas-resistance was examined using B cells from both kinds of mice. With xid B cells, the levels of Fas-sensitivity produced by CD40L stimulation, and of ...
View PDF 66.10 K
View PDF 66.10 K

Cell adhesion in plants is under the control of
Cell adhesion in plants is under the control of

... upstream promoter region of ESMD1 was then used to study the gene ...
Par-complex proteins promote proliferative
Par-complex proteins promote proliferative

... gain- and loss-of-function experiments. Par3 (Pard3) loss-of-function led to premature cell cycle exit, reflected in reduced clone size in vitro and the restriction of the progeny to the lower cortical layers in vivo. By contrast, Par3 or Par6 (Pard6␣) overexpression promoted the generation of Pax6+ ...
Coordination between Cell Growth and Cell Cycle Transit in Animal
Coordination between Cell Growth and Cell Cycle Transit in Animal

... cells, which make the "yes or no" decision in G~-pm about whether to continue through the cell cycle or not, have the capacity to decide, in Gl-ps, "when" they will enter the S phase. The differences in the kinetics between these two transitions (Gl-pm/Gl-ps vs. Gl-ps/ S) suggest the involvement of ...
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View

... P. crispum cells (Kauss et al., 1993; Franke et al., 1998), are in full accord with, and further confirm, the general conclusion (Hahlbrock et al., 2003) that the three analogous series of 4-hydroxy-, 4-hydroxy-3-methoxy-, and 4-hydroxy-3,5-dimethoxybenzaldehyde, -benzoate and -cinnamate together con ...
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats

... treatment schedule. About 5 to 7 days after the application of thyroxine, the rate of apoptosis of beta cells and ductal cells had returned to basal again and remained low even 14 days after the hormone application (Fig. 2A, B). The reduction of the beta-cell volume of the pancreas after thyroxine a ...
Endocytic Uptake of Nutrients, Cell Wall Molecules, and Fluidized
Endocytic Uptake of Nutrients, Cell Wall Molecules, and Fluidized

... Importantly, uptake of extracellular nutrients by endocytosis is not in direct conflict with transport through membrane-bound carriers given that cell homeostasis can be better maintained if both these mechanisms operate in parallel. For example, “reserve” sucrose to be accumulated in the vacuole is ...
Cell Wall
Cell Wall

... The cell wall is the tough, flexible but sometimes fairly rigid layer that surrounds some types of cells. It is located outside the cell membrane and provides these cells with structural support and protection, in addition to acting as a filtering mechanism. A major function of the cell wall is to a ...
Cell shrinkage and apoptosis: a role for potassium and sodium ion
Cell shrinkage and apoptosis: a role for potassium and sodium ion

... membranes, we are provided with convenient tools to directly measure cellular changes associated with apoptosis and to measure these changes on a single cell basis. Importantly, because of increasing evidence suggesting normal genetic regulators of cell proliferation and differentiation as regulator ...
REST/NRSF TARGET GENES IN NEURONAL AND BETA CELLS
REST/NRSF TARGET GENES IN NEURONAL AND BETA CELLS

... REST/NRSF is a Gli-Krüppel-like zinc finger transcription factor.10 Although the expression level of REST/NRSF is highly variable, it is widely expressed in most tissues of adult mice. In adult rats and mice, the lowest level of REST/NRSF mRNA is detected in the central nervous system (CNS) and panc ...
DeadEnd Fluorometric TUNEL System Technical
DeadEnd Fluorometric TUNEL System Technical

... In many cell types, apoptosis is characterized by the generation of DNA fragments through the action of endogenous endonucleases (11–14). The DNA of apoptotic cells is cleaved into multimers of 180–200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typ ...
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Programmed cell death



Programmed cell-death (or PCD) is death of a cell in any form, mediated by an intracellular program. PCD is carried out in a regulated process, which usually confers advantage during an organism's life-cycle. For example, the differentiation of fingers and toes in a developing human embryo occurs because cells between the fingers apoptose; the result is that the digits are separate. PCD serves fundamental functions during both plant and metazoa (multicellular animals) tissue development.Apoptosis and autophagy are both forms of programmed cell death, but necrosis is a non-physiological process that occurs as a result of infection or injury.Necrosis is the death of a cell caused by external factors such as trauma or infection and occurs in several different forms. Recently a form of programmed necrosis, called necroptosis, has been recognized as an alternate form of programmed cell death. It is hypothesized that necroptosis can serve as a cell-death backup to apoptosis when the apoptosis signaling is blocked by endogenous or exogenous factors such as viruses or mutations.
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