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Auxin metabolism and homeostasis during plant development
Auxin metabolism and homeostasis during plant development

... Although our understanding of auxin signalling has flourished during the last few decades, progress in understanding auxin metabolism (which includes auxin biosynthesis, conjugation and degradation) has been hampered by the lack of suitable tools for the quantification and visualisation of auxin met ...
Identification of genes that interact with glp-1, a gene
Identification of genes that interact with glp-1, a gene

Unipro UGENE Manual
Unipro UGENE Manual

... You're going to use only basic UGENE features and don't want to waste Internet traffic You have limited disk space Use the NGS package, if: You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched fr ...
Advanced Stochastic Protein Sequence Analysis
Advanced Stochastic Protein Sequence Analysis

... genes in the human genome is still not clear. In [Ven01] both a pessimistic gene number of 26 000 and a more optimistic figure of 40 000 is given. The Human Genome Consortium initially found evidence for approximately 30 000 transcripts and recently only 20 000 to 25 000 genes were supposed [IHG04]. ...
Unipro UGENE Manual
Unipro UGENE Manual

... You're going to use only basic UGENE features and don't want to waste Internet traffic You have limited disk space Use the NGS package, if: You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched fr ...
Literature Study
Literature Study

... disorders that can collectively be termed autism spectrum disorders (ASDs). The most common forms of ASD are classical autism, Asperger syndrome and pervasive developmental disorder-not otherwise specified (PDD-NOS)(Baltussen, Clijsen, Leenders 2003). Individuals with an ASD can be characterized by ...
Molecular and biochemical characterization of cytosolic
Molecular and biochemical characterization of cytosolic

... ADP, 3-phosphoglyceric acid, inorganic pyrophosphate, ribulose 1,5-bisphosphate, and inorganic orthophosphate) were added to the PGM assay to determine whether they caused an activation or inhibition of the PGM isoforms. However, it was found that none of these metabolites had any signi®cant effect ...
The growth hormone auxin
The growth hormone auxin

... negative regulators 2. Mutations in Aux /IAA confer dominant auxin resistance; probably increase the half-life of Aux /IAA proteins ...
Full-Text PDF
Full-Text PDF

... domain and three loops to interact with ubiquitin [30]. The ubiquitin binding region of NEDD4 HECT ubiquitin ligase interacts with ubiquitin by means of a tripod involving three distinct loops [23,31]. Two additional surfaces of ubiquitin, the Asp58-centered region [25,32], and the C-terminal surfac ...
dhaA - Queen`s University Belfast
dhaA - Queen`s University Belfast

... Molecular techniques now reveal hidden diversity Heterotrophs 5-20% biomass in sea waters Rich bacterial communities in sub-surface strata (600 m deep) • up to 2 x 1040 tons - more than all flora and fauna • equivalent up to 2 m layer over planet! ...
COLEOPTILE PHOTOTROPISM1 Gene Encoding
COLEOPTILE PHOTOTROPISM1 Gene Encoding

... the three clones (P0689H05, P0025F02, and OSJNBa0008E01) and the deletion region of 208,845 bp was determined (Figure 3A). Thirty-two putative genes were predicted in the deleted region with the Genscan program. A BLAST search of DNA databases indicated, however, that one of the putative genes (Figu ...
Chapter 27 Slides
Chapter 27 Slides

... Xanthine Oxidase and Gout • XO in liver, intestines (and milk) can oxidize hypoxanthine (twice) to uric acid • Humans and other primates excrete uric acid in the urine, but most N goes out as urea • Birds, reptiles and insects excrete uric acid and for them it is the major nitrogen excretory ...
Molecular characterization of a cluster of at least two
Molecular characterization of a cluster of at least two

... S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previously (Pitty et al., 1989). Bacteriophage L DNA was purified from 1 litre liquid lysates according to the method of Silhavy et al. (1984). Both the purification of plasmids from ...
Molecular characterization of a cluster of at least two
Molecular characterization of a cluster of at least two

... S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previously (Pitty et al., 1989). Bacteriophage L DNA was purified from 1 litre liquid lysates according to the method of Silhavy et al. (1984). Both the purification of plasmids from ...
METABOLIC CUES AND REGULATORY PROTEINS
METABOLIC CUES AND REGULATORY PROTEINS

... when various freshwater protozoa ingest L. pneumophila, the microbes can efficiently establish an intracellular niche protected from digestion. Moreover, if humans inhale bacteria-laden aerosols, L. pneumophila can survive and replicate within alveolar macrophages to cause the severe pneumonia, Legi ...
Evidence Suggesting that Pif1 Helicase Functions in DNA
Evidence Suggesting that Pif1 Helicase Functions in DNA

... viable and repair proficient. Both yeast proteins have 5⬘to-3⬘ DNA helicase activity (21, 30, 31). The region of similarity between Pif1 and Rrm3 is limited to the seven helicase motifs, which exhibit 40% identity and 60% similarity (3). This may indicate that the two helicases have structurally sim ...
Ingenuity Variant Analysis
Ingenuity Variant Analysis

... for all four family members, a SNP which has been repeatedly reported as being pathogenic for PRSS1-related Hereditary Pancreatitis. As mentioned in the previous report, we assumed incomplete penetrance contributed to the fact that none of the family members were affected. However, the results from ...
ETHYLENE
ETHYLENE

... Lorenzo, O., Piqueras, R., Sanchez-Serrano, J.J., and Solano, R. (2003). ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15: 165-178; Rüžička, K., Ljung, K., Vanneste, S., Podhorská, R., Beeckman, T., Friml, J., and Benková, E. (2007). E ...
click here - Merle Cockers
click here - Merle Cockers

... doesn't weaken anything down the line unless there are other issues that would be regular problems anyway. Q: How can there not be a recessive gene "ever"? What scientific tests are available to confirm this statement? XXXXX A: There's so much new research on genetics and DNA testing is certainly go ...
Letters in Applied Microbiology
Letters in Applied Microbiology

... ultimum and protect cucumber from P. ultimum-mediated damping-off (Rezzonico et al. 2005), suggesting that effectors (not identified so far) secreted via this system could be involved in biocontrol. As strains from P. fluorescens and related species colonize the rhizosphere aggressively, competition ...
Systems Biotechnology of Pseudomonas putida for the enhanced
Systems Biotechnology of Pseudomonas putida for the enhanced

... sustainable alternatives to replace petroleum-base commodities. These biopolymers have superior features than synthetic plastics since they are produced from biobased sources and their intrinsic chemical composition make them biodegradable, biocompatible, and part of the carbon cycle of the earth. A ...
From Sequence to Structure
From Sequence to Structure

ABSTRACT Title of Document:
ABSTRACT Title of Document:

... and abiotic stresses. To find new members of the ethylene signaling pathway, a genetic screen for suppressors of the ethylene-insensitive mutant etr1-2 was performed. One mutant identified in this screen, etr1-11, is an intragenic mutation within ETR1. etr1-11 is a unique missense mutation that appe ...
2.1.databases_intro - T
2.1.databases_intro - T

... •Write down the PubMed Identifier (the number in the PMID field) of that interesting paper you just find. It could be very useful in your subsequent search for related items such as associated gene and protein sequences ...
ADRC2010_GetTheMostOutofFlyBase
ADRC2010_GetTheMostOutofFlyBase

... www.flybase.org ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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