Diagnostic protocol for
... (PVP) vortex and left for 1 h at room temperature with continuous shaking. The suspension is centrifuged at 5000 g for 5 min, 450 µl of the supernatant is transferred and 450 µl isopropanol is added. The suspension is mixed gently and left at room temperature for 1 h. Precipitation can be improved b ...
... (PVP) vortex and left for 1 h at room temperature with continuous shaking. The suspension is centrifuged at 5000 g for 5 min, 450 µl of the supernatant is transferred and 450 µl isopropanol is added. The suspension is mixed gently and left at room temperature for 1 h. Precipitation can be improved b ...
Macrocyclic Carbohydrate/Amino Acid Hybrid Molecules
... be utilized as chemosensors7-10 and be used analogously to pH indicators to detect and quantify analytes. Recently, sensing systems that rely on the displacement of an indicator from a receptor have been developed. These systems have been used for the detection of phosphate11 and pyrophosphate12 ion ...
... be utilized as chemosensors7-10 and be used analogously to pH indicators to detect and quantify analytes. Recently, sensing systems that rely on the displacement of an indicator from a receptor have been developed. These systems have been used for the detection of phosphate11 and pyrophosphate12 ion ...
Noureddine Loukili Studies on UEV, a new regulator of polyubiquitination:
... I.1. Discovery of ubiquitin About 27 years ago, Goldstein isolated a protein which he identified as a lymphocyte differentiation promoting factor, and which he called ubiquitin (Goldstein and Dayhoff, 1975) as he thought it was probably ubiquitous to living cells. A few years later, the non-histone ...
... I.1. Discovery of ubiquitin About 27 years ago, Goldstein isolated a protein which he identified as a lymphocyte differentiation promoting factor, and which he called ubiquitin (Goldstein and Dayhoff, 1975) as he thought it was probably ubiquitous to living cells. A few years later, the non-histone ...
- Wiley Online Library
... (Sussman et al., 2000), a vas insertional mutant was isolated harbouring an inverted T-DNA repeat insert (LBRB:RB-LB) 268 bp downstream of the protein translation start site (Figure 2a,b). RT-PCR analysis confirmed complete loss-of-function for VAS, while LEP expression was unaffected. The developme ...
... (Sussman et al., 2000), a vas insertional mutant was isolated harbouring an inverted T-DNA repeat insert (LBRB:RB-LB) 268 bp downstream of the protein translation start site (Figure 2a,b). RT-PCR analysis confirmed complete loss-of-function for VAS, while LEP expression was unaffected. The developme ...
Idic(15)
... The Unique experience is that new babies tend to feed slowly and breastfeeding may be hard to establish. The hypotonia that is common in babies with idic(15) can lead to difficulties with sucking and swallowing, and/or latching onto the breast. Babies with a cleft or high palate can also find the ac ...
... The Unique experience is that new babies tend to feed slowly and breastfeeding may be hard to establish. The hypotonia that is common in babies with idic(15) can lead to difficulties with sucking and swallowing, and/or latching onto the breast. Babies with a cleft or high palate can also find the ac ...
biology syllabus
... Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. ...
... Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. ...
scope and use of bioinformatics in genomic and proteomic
... Modelling of macromolecular systems – deducing properties from atomic interactions Modelling of complex systems – protein interactions, pathways, regulatory networks, whole organ models: “Systems Biology” Institut Suisse de Bioinformatique Schweizerisches Institut für Bioinformatik Swiss Institute o ...
... Modelling of macromolecular systems – deducing properties from atomic interactions Modelling of complex systems – protein interactions, pathways, regulatory networks, whole organ models: “Systems Biology” Institut Suisse de Bioinformatique Schweizerisches Institut für Bioinformatik Swiss Institute o ...
(reversed and/or heterotaxic) phenotype in SWV mice
... small closed colony and eventually an inbred strain was produced by brother-sister mating for 20 generations. This strain is called SIViLay provisionally (SIV in this paper). Inbred strains AiJ, AKRIJ, BALHlcJ, CBAiJ, C57BLi6J, and DBAl2J were obtained from The Jackson Laboratory and SWV from F.G.B. ...
... small closed colony and eventually an inbred strain was produced by brother-sister mating for 20 generations. This strain is called SIViLay provisionally (SIV in this paper). Inbred strains AiJ, AKRIJ, BALHlcJ, CBAiJ, C57BLi6J, and DBAl2J were obtained from The Jackson Laboratory and SWV from F.G.B. ...
... pairs were used, regardless of parental genotyping (SibPhase), the maximum LOD score was 1.9 near the markers DlS2614 and DlS1588 with 59.8% allele sharing. Consistent with the previous report by Reich et al. (1998), there was a second linkage finding with a maximum LOD score of 1.7 near the markers ...
Canadian journal of Microbiology
... causing significant loss of economically important crops (Purdy 1979). Because there is no known resistance against S. sclerotiorum in commercially grown canola cultivars, management of this pathogen is essential. Public concern over the use of chemical pesticides together with the potential for acq ...
... causing significant loss of economically important crops (Purdy 1979). Because there is no known resistance against S. sclerotiorum in commercially grown canola cultivars, management of this pathogen is essential. Public concern over the use of chemical pesticides together with the potential for acq ...
Real-time PCR Handbook
... choose an RT that not only provides high yields of full-length cDNA, but also has good activity at high temperatures. High-temperature performance is also very important for denaturation of RNA with secondary structure. In one-step qRT-PCR, an RT that retains its activity at higher temperatures allo ...
... choose an RT that not only provides high yields of full-length cDNA, but also has good activity at high temperatures. High-temperature performance is also very important for denaturation of RNA with secondary structure. In one-step qRT-PCR, an RT that retains its activity at higher temperatures allo ...
Dorsoventral Patterning in Hemichordates
... expressed in the three germ layers of hemichordate embryos in distinct dorsoventral domains, such as pox neuro, pituitary homeobox, distalless, and tbx2/3 on the Bmp side and netrin, mnx, mox, and single-minded on the ChordinAdmp side. When we expose the embryo to excess Bmp protein, or when we depl ...
... expressed in the three germ layers of hemichordate embryos in distinct dorsoventral domains, such as pox neuro, pituitary homeobox, distalless, and tbx2/3 on the Bmp side and netrin, mnx, mox, and single-minded on the ChordinAdmp side. When we expose the embryo to excess Bmp protein, or when we depl ...
22q12 and 22q13 duplications
... shows gains and losses of tiny amounts of DNA throughout the chromosomes. Microarrays can also show whether particular genes are duplicated or not. The break points in chromosome 22 vary from person to person, so each person will have a different number of genes duplicated. The smallest duplications ...
... shows gains and losses of tiny amounts of DNA throughout the chromosomes. Microarrays can also show whether particular genes are duplicated or not. The break points in chromosome 22 vary from person to person, so each person will have a different number of genes duplicated. The smallest duplications ...
Resistance to hydrogen peroxide in Helicobacter pylori
... PCR followed using the following parameters : 94 mC for 4 min and a cycle of 94 mC for 10 s, 50 mC for 10 s and 72 mC for 1 min, repeated 35 times. A final elongation step of 72 mC for 7 min followed. RT-PCR products were analysed using agarose gel electrophoresis. Polymerase chain reaction. Fragmen ...
... PCR followed using the following parameters : 94 mC for 4 min and a cycle of 94 mC for 10 s, 50 mC for 10 s and 72 mC for 1 min, repeated 35 times. A final elongation step of 72 mC for 7 min followed. RT-PCR products were analysed using agarose gel electrophoresis. Polymerase chain reaction. Fragmen ...
SPATULA, a bHLH carpel development gene
... male gametophyte from the exterior to the ovules located within. These tissues usually develop from the carpel margins and include a stigma on which pollen alights, and transmitting tissues within the style and ovary through which the pollen tubes grow. In Arabidopsis, two congenitally joined carpel ...
... male gametophyte from the exterior to the ovules located within. These tissues usually develop from the carpel margins and include a stigma on which pollen alights, and transmitting tissues within the style and ovary through which the pollen tubes grow. In Arabidopsis, two congenitally joined carpel ...
Characterisation of interstitial duplications and triplications of
... Characterisation of breakpoints Breakpoint analysis was performed by using PCR at microsatellite loci throughout 15q11-q14 and the results for all 18 families are shown in Fig. 1. The interpretation of results for families 5 and 8 was complex (see later) but, including these families, all duplicatio ...
... Characterisation of breakpoints Breakpoint analysis was performed by using PCR at microsatellite loci throughout 15q11-q14 and the results for all 18 families are shown in Fig. 1. The interpretation of results for families 5 and 8 was complex (see later) but, including these families, all duplicatio ...
Histological classification and molecular genetics of meningiomas
... of characteristic uniform tumour cells that form lobules surrounded by thin collagenous septae. Within the lobules, epithelioid tumour cells have fuzzy ill-defined cell borders that resemble a syncytium. Characteristic nuclear changes include clear spaces (that seem empty of karyoplasm) and rounded e ...
... of characteristic uniform tumour cells that form lobules surrounded by thin collagenous septae. Within the lobules, epithelioid tumour cells have fuzzy ill-defined cell borders that resemble a syncytium. Characteristic nuclear changes include clear spaces (that seem empty of karyoplasm) and rounded e ...
Bacterial Gene Expression—Lac Operon
... • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditions positively or negatively regulate the operon? • What are the differe ...
... • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditions positively or negatively regulate the operon? • What are the differe ...
GC-content of synonymous codons profoundly influences amino
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
Regulation of transcript encoding the 43K
... with negative numbers. The number of the nucleotide residue at the end of each line is provided. The predicted amino acid sequence is shown above the nucleotide sequence. Amino acid residues are numbered beginning with the amino terminal residue. The 5' and 3' ends of the Xen 43.1 cDNA is bordered b ...
... with negative numbers. The number of the nucleotide residue at the end of each line is provided. The predicted amino acid sequence is shown above the nucleotide sequence. Amino acid residues are numbered beginning with the amino terminal residue. The 5' and 3' ends of the Xen 43.1 cDNA is bordered b ...
Lac Operon - Faculty Web Pages
... • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditions positively or negatively regulate the operon? • What are the differe ...
... • What is an operon? How is it different from a eukaryotic gene? • In the lac operon, what cellular or environmental conditions must exist in order for the (WT) lac operon to express its genes? How do these environmental conditions positively or negatively regulate the operon? • What are the differe ...
as a PDF
... weak acid NH^ . Thus, a daily excretion of 30 g urea in man is equivalent to the disposal of l mole HCO^. Ammonium ions, however, can also be detoxified by glutamine formation, a process occurring in the liver äs well äs in other organs, and ammoniurn can be excreted into urine äs such after renal h ...
... weak acid NH^ . Thus, a daily excretion of 30 g urea in man is equivalent to the disposal of l mole HCO^. Ammonium ions, however, can also be detoxified by glutamine formation, a process occurring in the liver äs well äs in other organs, and ammoniurn can be excreted into urine äs such after renal h ...