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labs.bio.unc.edu
labs.bio.unc.edu

... Population size ...
Horizontal gene transfer from flowering plants to Gnetum
Horizontal gene transfer from flowering plants to Gnetum

... neighbor-joining, with sequence divergences calculated under the GTR ⫹ G ⫹ I model (25) after removing all gaps and ambiguous characters. Nonparametric bootstrap support was obtained by resampling the data 1,000 times with the same search options and model. Parsimony analyses of full intron sequence ...
Global transcriptional control by glucose and
Global transcriptional control by glucose and

... in the glucose-dependent repression of C. difficile toxin production. This repression is because of a direct binding of CcpA to the regulatory region of the tcdA and tcdB genes (30). This is the first evidence that CcpA acts as a link between carbon source utilization and virulence gene expression in ...
Full Text  - G3: Genes | Genomes | Genetics
Full Text - G3: Genes | Genomes | Genetics

... wild type), we used the AV221 strain to visualize homologous chromosome pairing during meiotic prophase in the germlines of live worms. AV221 worms exhibit GFP signals in meiotic prophase nuclei in the gonads of live worms (Figure 1), beginning in the TZ, where germ cells enter meiotic prophase and ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

... 3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
Journal Club Pack - Circulation Research
Journal Club Pack - Circulation Research

... SMC Phenotypic Switching In Vivo Morgan Salmon, Delphine Gomez, Elizabeth Greene, Laura Shankman, and Gary K. Owens ...
University of Groningen Lactococcus lactis systems biology Eckhardt
University of Groningen Lactococcus lactis systems biology Eckhardt

Novel Regulation of Cardiac Metabolism and Homeostasis by the
Novel Regulation of Cardiac Metabolism and Homeostasis by the

... embryonically lethal with cardiovascular abnormalities that are the same as AM−/−. This suggests that the AM-RAMP2 system is particularly important for the cardiovascular system. Although AM and RAMP2 are highly expressed in the heart from embryo to adulthood, their analysis has been limited by the ...
Full-Text PDF
Full-Text PDF

... importance of secondary structure and MTG’s apparent preference for flexible polypeptide regions, the reactivity of MTG towards apoMb, α-lactalbumin (α-LA) and fragment 205-316 of thermolysin was analyzed [53]. These extensively studied proteins are models of α-helices, β-sheets and unstructured reg ...
Identification of a Chloroplast-encoded 9-kDa
Identification of a Chloroplast-encoded 9-kDa

... It remains to be established whether the reported spectra could have been derived from an artificial complex of similar molecular mass and generated from cysteine, sulfide, and iron. As demonstrated by Hej and Meller (ll), the coincidental comigration of such artificially formed oligomeric complexes ...
Apolipoprotein E Allele Distribution in Trisomy
Apolipoprotein E Allele Distribution in Trisomy

... syndrome and healthy control subjects may help explain and support previous work that did not find high rates of atherosclerosis among these persons. The role of apoE alleles in the development of trisomies needs further study. ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748

... Total RNA was isolated from each biopsy sample, treated with deoxyribonuclease (Ambion) to remove contaminating DNA, and treated with RNA Secure (Ambion) to inactivate ribonucleases. Quality of RNA was verified by inspection of the 18S and 28S rRNA bands after agarose gel electrophoresis. The Strata ...
PDF
PDF

... (44% identity) [11] and from the moderately thermophilic, methanogenic archaeon, Methanosarcina thermophila (38% identity) [14]. However, systematic analyses of the amino acid sequences of analogous mesophilic, moderately thermophilic and hyperthermophilic proteins, even in the case of small redox p ...
Chemistry 433 BIOCHEMISTRY LABORATORY MANUAL
Chemistry 433 BIOCHEMISTRY LABORATORY MANUAL

... All students will be required to maintain a laboratory notebook. The notebook will be used for the recording of laboratory data and calculations, and will be a critically important source of information while writing your lab reports. The purpose of a biochemistry laboratory notebook is to allow any ...
Impaired glycogen synthesis causes metabolic
Impaired glycogen synthesis causes metabolic

... Revised ...
Ribosome engineering to promote new crystal forms
Ribosome engineering to promote new crystal forms

... Even though the crystal forms belong to different space groups and are from different species, e.g. T. thermophilus (Selmer et al., 2006) or E. coli (Schuwirth et al., 2005), the overall regions of contact between L9 and the neighbouring 30S subunit are similar. The 82–93 and 121–122 loop regions in ...
French 2003 - University of Washington
French 2003 - University of Washington

... general, however, much less is known about the genetic pathways regulating these molecules and the link between cell fate-determining processes and cytoskeletal functions. We are using dorsal appendage morphogenesis in Drosophila oogenesis as a model system for studying the relationship between patt ...
CCA Summer 2004 Newsletter - Children`s Craniofacial Association
CCA Summer 2004 Newsletter - Children`s Craniofacial Association

... of a pair that is unchanged and working properly and one gene that is changed and is not working properly. The gene that is altered “dominates” over the unchanged gene, causing the features of CCD. The gene that causes CCD is denoted by the symbol RUNX2, and it directs the body to make a protein tha ...
34386 - PubAg
34386 - PubAg

... specific blend of terpenoids and indole that is attractive to Cortesia marginiventris, a parasitic wasp that attacks larvae of several species of Lepidoptera. Two elicitors have been identified in the oral secretions of insect herbivores that trigger the production of volatiles. Mattiacci et al. (8) ...
Autism and maternally derived aberrations of chromosome 15q
Autism and maternally derived aberrations of chromosome 15q

... probe at 15q22 was not present on the marker. The marker was identified as a supernumerary chromosome 15 with a terminal deletion at q14. The karyotype was designated 47,XY,+del(15)(q14). Parental karyotypes were normal. The quantitative Southern blot assay confirmed the cytogenetic findings (Table ...
Module 3
Module 3

... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
Introns Structure Patterns of Variation in Nucleotide Composition in
Introns Structure Patterns of Variation in Nucleotide Composition in

... (expressed sequence tags) or Full Length cDNA (FLcDNA) are available, it is indicated. We kept only gene models supported by full length cDNA. The same data as for A. thaliana were recorded for each selected gene model. ...
Tunicates and not cephalochordates are the closest living relatives
Tunicates and not cephalochordates are the closest living relatives

... particular attention to potential systematic biases associated, for instance, with differences in evolutionary rates among species, compositional biases and heterotachy8. In particular, a long-branch attraction (LBA) artefact14 may potentially occur since tunicates include fast (Ciona ssp.) and very ...
Gluconeogenesis
Gluconeogenesis

PCR Clean-up Kit / 96-well PCR Clean
PCR Clean-up Kit / 96-well PCR Clean

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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