The molecular genetics of von Willebrand disease
... (type 2B) or abrogate (type 2M) the potential for interacting with the GPIb platelet receptor.34 To date, 22 different missense mutations have resulted in type 2B VWD and one 2B mutation represents the insertion of a methionine residue. All of these changes are clustered in the A1 domain of the prot ...
... (type 2B) or abrogate (type 2M) the potential for interacting with the GPIb platelet receptor.34 To date, 22 different missense mutations have resulted in type 2B VWD and one 2B mutation represents the insertion of a methionine residue. All of these changes are clustered in the A1 domain of the prot ...
SEX-DETector: a probabilistic approach to uncover sex
... a dioecious progenitor (Ming et al., 2011). However, the mechanisms for sex determination remain unknown for most plant species and a number of animal species (Bachtrog, Mank, et al., 2014). In numerous cases, it is even unknown whether sex chromosomes are present. In angiosperms, dioecy has evolved ...
... a dioecious progenitor (Ming et al., 2011). However, the mechanisms for sex determination remain unknown for most plant species and a number of animal species (Bachtrog, Mank, et al., 2014). In numerous cases, it is even unknown whether sex chromosomes are present. In angiosperms, dioecy has evolved ...
Co-translational Folding
... – The kinetics of the folding process, the partitioning of polypeptides among alternative forms, and the yield of correctly folded protein are consequences of kinetic partitioning between alternative pathways. – When proteins do not fold correctly (i.e. "misfold"), there can be serious consequences, ...
... – The kinetics of the folding process, the partitioning of polypeptides among alternative forms, and the yield of correctly folded protein are consequences of kinetic partitioning between alternative pathways. – When proteins do not fold correctly (i.e. "misfold"), there can be serious consequences, ...
VCR 221 - Potato - UC Davis Plant Sciences
... Hosaka, K and Hanneman Jr. R. 1998. Genetics of self-incompatibilty in a selfincompatible potato species Solanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene in the potato genome using DNA markers. Euphytica 103:265-271. Hosaka K 2002. Distribution of a 241 bp chloroplast DNA deleti ...
... Hosaka, K and Hanneman Jr. R. 1998. Genetics of self-incompatibilty in a selfincompatible potato species Solanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene in the potato genome using DNA markers. Euphytica 103:265-271. Hosaka K 2002. Distribution of a 241 bp chloroplast DNA deleti ...
LIST OF CHECK-UP QUESTIONS for
... Answers multiple tests, choose and indicate the correct answer/statement, please: 1. The non-membranous organelles are: a) ribosomes; CORRECT b) Golgi body; c) lysosomes; d) Endoplasmic reticulum; e) nucleus 2. Chloroplasts and mitochondria both NOT: a) are found in plant cells; b) have two membrane ...
... Answers multiple tests, choose and indicate the correct answer/statement, please: 1. The non-membranous organelles are: a) ribosomes; CORRECT b) Golgi body; c) lysosomes; d) Endoplasmic reticulum; e) nucleus 2. Chloroplasts and mitochondria both NOT: a) are found in plant cells; b) have two membrane ...
The Normal Uniform Differential Gene Expression
... differentially expressed group are computed using the estimated model parameters. Typically a threshold for probability of being expressed of 0.5 is used for classification of the genes into the two groups (≥ 0.5 = differentially expressed, < 0.5 = not differentially expressed) but the probabilities ...
... differentially expressed group are computed using the estimated model parameters. Typically a threshold for probability of being expressed of 0.5 is used for classification of the genes into the two groups (≥ 0.5 = differentially expressed, < 0.5 = not differentially expressed) but the probabilities ...
MeCP2 mutations in children with and without
... Article abstract—Background: Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the X-linked methyl CpG binding protein 2 (MeCP2) gene. Methods: One hundred sixteen patients with classical and atypical RTT were studied for mutations of the MeCP2 gene by using DHPLC and direc ...
... Article abstract—Background: Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the X-linked methyl CpG binding protein 2 (MeCP2) gene. Methods: One hundred sixteen patients with classical and atypical RTT were studied for mutations of the MeCP2 gene by using DHPLC and direc ...
In-class assignment: Fukuda et al. (2016) paper
... embryos that express Kdm4b and are treated with TSA? How do Kdm4b and TSA affect the levels of chromatin ‘relaxation’? How does this relate to Xist expression and XCI? The chromatin around Xist loci on the maternal chromosomes is highly condensed in early XmXm embryos. Embryos that express Kdm4b and ...
... embryos that express Kdm4b and are treated with TSA? How do Kdm4b and TSA affect the levels of chromatin ‘relaxation’? How does this relate to Xist expression and XCI? The chromatin around Xist loci on the maternal chromosomes is highly condensed in early XmXm embryos. Embryos that express Kdm4b and ...
Supp Mat - Columbia University
... overexpressing strain kindly provided by Prof. Ya-Ming Hou and Dr. Marcel Dupasquier. The measured aminoacylation efficiencies were then applied to the total tRNA concentration, after correcting for the presence of the Flexizyme, to obtain the final aatRNA concentrations for translation reactions. T ...
... overexpressing strain kindly provided by Prof. Ya-Ming Hou and Dr. Marcel Dupasquier. The measured aminoacylation efficiencies were then applied to the total tRNA concentration, after correcting for the presence of the Flexizyme, to obtain the final aatRNA concentrations for translation reactions. T ...
Hox patterning of the vertebrate axial skeleton
... plan, wherein the identity of parasegments along the AP axis normally expressing a specific Hox gene are transformed to the identity of parasegments immediately anterior to the loss-offunction region (Lewis, 1963, 1978). The reciprocal gain-of-function experiments in Drosophila, in which more posteri ...
... plan, wherein the identity of parasegments along the AP axis normally expressing a specific Hox gene are transformed to the identity of parasegments immediately anterior to the loss-offunction region (Lewis, 1963, 1978). The reciprocal gain-of-function experiments in Drosophila, in which more posteri ...
Reasons for the occurrence of the twenty coded protein amino acids
... (c) The free amino acid and its side chain in a peptide should be adequately stable in solution. If an amino acid decomposes too rapidly on the geological time-scale, its steady state concentration would be too low for effective use in primitive peptides and in the most primitive organisms. An examp ...
... (c) The free amino acid and its side chain in a peptide should be adequately stable in solution. If an amino acid decomposes too rapidly on the geological time-scale, its steady state concentration would be too low for effective use in primitive peptides and in the most primitive organisms. An examp ...
Malate Dehydrogenase
... al. 1987). Leaf peroxisomes as well as seed glyoxysomes contain a large amount of activity of an isoenzyme of NAD-malate dehydrogenase (Yamazaki and Tolbert 1969; Curry and Ting 1973). Besides catalase (an enzyme that constitutes up to 40 percent of the total peroxisomal protein), malate dehydrogena ...
... al. 1987). Leaf peroxisomes as well as seed glyoxysomes contain a large amount of activity of an isoenzyme of NAD-malate dehydrogenase (Yamazaki and Tolbert 1969; Curry and Ting 1973). Besides catalase (an enzyme that constitutes up to 40 percent of the total peroxisomal protein), malate dehydrogena ...
Synthesis and Characterisation of N
... I hereby certify that I am the sole author of this thesis and that no part of this thesis has been published or submitted for publication. I certify that, to the best of my knowledge, my thesis does not infringe upon anyone’s copyright nor violate any proprietary rights and that any ideas, technique ...
... I hereby certify that I am the sole author of this thesis and that no part of this thesis has been published or submitted for publication. I certify that, to the best of my knowledge, my thesis does not infringe upon anyone’s copyright nor violate any proprietary rights and that any ideas, technique ...
DNA supercoiling factor contributes to dosage
... Knockdown of SCF severely affects male viability To analyze the biological function of SCF, we attempted to perform RNA interference (RNAi) in vivo. Recently, a method developed to express dsRNA as an extended hairpin loop was shown to be successful for RNAi-based gene knockdown in the nematode Caen ...
... Knockdown of SCF severely affects male viability To analyze the biological function of SCF, we attempted to perform RNA interference (RNAi) in vivo. Recently, a method developed to express dsRNA as an extended hairpin loop was shown to be successful for RNAi-based gene knockdown in the nematode Caen ...
Molecular Characterization of Visual Pigments in
... eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentration of 1 PCR Buffer minus Mg (Invitrogen), 0.2 mM of deoxynucleoside triphosphate (dNTP) mixture which contains all 4 deoxynucleotides (dATP, dCTP, dGTP, and dTTP), an ...
... eyes and ocelli. Clone development was achieved by PCR using 0.5 U of Platinum Taq DNA polymerase (Invitrogen) at a final concentration of 1 PCR Buffer minus Mg (Invitrogen), 0.2 mM of deoxynucleoside triphosphate (dNTP) mixture which contains all 4 deoxynucleotides (dATP, dCTP, dGTP, and dTTP), an ...
DNA Evolution 3.1 Troubleshooting and Debugging Guide
... Since the system logging system is not available on Windows. You can enable logging using the dbgview.exe application. This is a Windows debugging tool that's available from the following link: http://download.sysinternals.com/files/DebugView.zip You can simple download the application and run it. N ...
... Since the system logging system is not available on Windows. You can enable logging using the dbgview.exe application. This is a Windows debugging tool that's available from the following link: http://download.sysinternals.com/files/DebugView.zip You can simple download the application and run it. N ...
Three-Dimensional Algebraic Models of the tRNA Code and 12
... (H-tRNA-C). The tRNA code that is considered to be Standard comprises 46 tRNAs, due to the absence of triplets with the base A in the 5’ position and of the tRNAs corresponding to the stop codons; the initiator tRNA is added to the elongation set of 45 [16]. Wobbling anticodons recognize more than o ...
... (H-tRNA-C). The tRNA code that is considered to be Standard comprises 46 tRNAs, due to the absence of triplets with the base A in the 5’ position and of the tRNAs corresponding to the stop codons; the initiator tRNA is added to the elongation set of 45 [16]. Wobbling anticodons recognize more than o ...
invited review
... apparent size of a 290-kDa homotetramer (60). However, radiation decreased the size of SGLT1 protein (detected on Western blots) with an apparent size of 70 kDa. The explanation for the higher estimate (290 kDa) by irradiation inactivation is not clear, but similar discrepancies have been reported f ...
... apparent size of a 290-kDa homotetramer (60). However, radiation decreased the size of SGLT1 protein (detected on Western blots) with an apparent size of 70 kDa. The explanation for the higher estimate (290 kDa) by irradiation inactivation is not clear, but similar discrepancies have been reported f ...
The Early Interaction of the Outer Membrane Protein PhoE with
... action with trigger factor, a cytoplasmic chaperone. Using a cross-linking approach, the latter protein was found to interact with nascent PhoE chains as short as 57 amino acids (1–3). Subsequently, at a late co-translational or early post-translational stage the precursor protein interacts with Sec ...
... action with trigger factor, a cytoplasmic chaperone. Using a cross-linking approach, the latter protein was found to interact with nascent PhoE chains as short as 57 amino acids (1–3). Subsequently, at a late co-translational or early post-translational stage the precursor protein interacts with Sec ...
Variant Effect Predictor Web interface Documentation
... Filtering options The VEP allows you to pre-filter your results e.g. by MAF or consequence type. Note that it is also possible to perform equivalent operations on the results page for the VEP, so if you aren't sure, don't use any of these options! By frequency - filter variants by minor allele frequ ...
... Filtering options The VEP allows you to pre-filter your results e.g. by MAF or consequence type. Note that it is also possible to perform equivalent operations on the results page for the VEP, so if you aren't sure, don't use any of these options! By frequency - filter variants by minor allele frequ ...
Potential alteration of U37K paleothermometer due to selective
... growth and biodiversity and the others for the determina0 tion of UK37 values and identification of any metabolites produced during the different incubation times. In order to monitor the degradation of alkenones by the different bacteria, sterile controls were prepared in the same way as the bacter ...
... growth and biodiversity and the others for the determina0 tion of UK37 values and identification of any metabolites produced during the different incubation times. In order to monitor the degradation of alkenones by the different bacteria, sterile controls were prepared in the same way as the bacter ...
Alarmingly High Segregation Frequencies of Quinolone Resistance
... In many metagenomic studies, large assemblages of bacteria are sequenced directly from their natural environments. In such studies, high-throughput sequencing techniques are used to sequence relatively short snippets of the genomes of the bacteria residing within an environment. These short reads th ...
... In many metagenomic studies, large assemblages of bacteria are sequenced directly from their natural environments. In such studies, high-throughput sequencing techniques are used to sequence relatively short snippets of the genomes of the bacteria residing within an environment. These short reads th ...
Structure and Function of Mammalian Ribosomes
... In our studies we concentrated our efforts on developing methods for the preparation of high yields of subunits, the majority of which are structurally and functionally intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the co ...
... In our studies we concentrated our efforts on developing methods for the preparation of high yields of subunits, the majority of which are structurally and functionally intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the co ...
Amino Acids
... carbon atom • There are 20 common amino acids • Amino acids can join via peptide bonds • Several amino acids occur only rarely in proteins • Some amino acids are not found in proteins ...
... carbon atom • There are 20 common amino acids • Amino acids can join via peptide bonds • Several amino acids occur only rarely in proteins • Some amino acids are not found in proteins ...
Ectopic induction and reorganization of Wnt-1
... embryonic met-mesencephalon seems to evolve as a unified region. In particular, this entire domain, but no other region of the neural tube, specifically expresses the homeobox-containing genes of the engrailed class, En-1 and En-2, since early stages of neurulation (Davis and Joyner, 1988; Davis et ...
... embryonic met-mesencephalon seems to evolve as a unified region. In particular, this entire domain, but no other region of the neural tube, specifically expresses the homeobox-containing genes of the engrailed class, En-1 and En-2, since early stages of neurulation (Davis and Joyner, 1988; Davis et ...