Complete genome sequence of the prototype lactic acid bacterium
... genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 gaps, in combination with inverse PCR for the gaps for which no linkage info ...
... genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 gaps, in combination with inverse PCR for the gaps for which no linkage info ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
Further manipulation by centric misdivision of the 1RS.1BL
... The experiments with repeated centric fissionfusion cycles demonstrate that chromosome arms can be transferred from one translocation to another until the most suitable location is found. As a result of the manipulations described here, translocations of the same rye chromosome arm 1RS to all three ...
... The experiments with repeated centric fissionfusion cycles demonstrate that chromosome arms can be transferred from one translocation to another until the most suitable location is found. As a result of the manipulations described here, translocations of the same rye chromosome arm 1RS to all three ...
Seed Germination of GA-Insensitive sleepy1
... each allele on SLY1 mRNA levels was examined by RT-PCR using SLY1 gene-specific primers (Figure 1B). When the SLY1specific primers were employed, no significant change in mRNA accumulation was observed in sly1-10 and sly1-2, whereas a marked decrease was seen in sly1-t2. These results indicate that ...
... each allele on SLY1 mRNA levels was examined by RT-PCR using SLY1 gene-specific primers (Figure 1B). When the SLY1specific primers were employed, no significant change in mRNA accumulation was observed in sly1-10 and sly1-2, whereas a marked decrease was seen in sly1-t2. These results indicate that ...
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... Page(s): 58 42. Emma and Anna are identical twins who were adopted by different families a few weeks after birth. Although genetically identical, they grew up with different physical and psychological characteristics. For example, though both inherited a tendency to grow large, Anna was slim and ath ...
... Page(s): 58 42. Emma and Anna are identical twins who were adopted by different families a few weeks after birth. Although genetically identical, they grew up with different physical and psychological characteristics. For example, though both inherited a tendency to grow large, Anna was slim and ath ...
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... Page(s): 58 42. Emma and Anna are identical twins who were adopted by different families a few weeks after birth. Although genetically identical, they grew up with different physical and psychological characteristics. For example, though both inherited a tendency to grow large, Anna was slim and ath ...
... Page(s): 58 42. Emma and Anna are identical twins who were adopted by different families a few weeks after birth. Although genetically identical, they grew up with different physical and psychological characteristics. For example, though both inherited a tendency to grow large, Anna was slim and ath ...
Additional Table 1. Genotype and phenotype of the
... kb, 14,823,878-15,041,954, genomic yes build hg 18). One gene involved (MACROD2 ). No parental studies were performed. The clinical significance of these copy number variants is unclear. ...
... kb, 14,823,878-15,041,954, genomic yes build hg 18). One gene involved (MACROD2 ). No parental studies were performed. The clinical significance of these copy number variants is unclear. ...
PKU at a Glance
... inability to metabolize PKU exists from the time the infant is in the womb. Infants are screened for PKU by a blood test shortly after ...
... inability to metabolize PKU exists from the time the infant is in the womb. Infants are screened for PKU by a blood test shortly after ...
1999 Dekkers: BREEDING IN THE 21st CENTURY
... the effect of the g’ene was known, compared selection on the QTL to selection on theanimal’s own phenotype, and assumed phenotype was observed on all animals. Other studies have removed one or more of these limitations by considering selection on linked genetic markers or marker braekets, selection ...
... the effect of the g’ene was known, compared selection on the QTL to selection on theanimal’s own phenotype, and assumed phenotype was observed on all animals. Other studies have removed one or more of these limitations by considering selection on linked genetic markers or marker braekets, selection ...
Saccharomyces species in the Production of Beer
... production of beer were water, barley, and hops. The principal purpose of the law was to prevent of beer were water, barley, and hops. The principal purpose of the law was to prevent price competition price competition with bakers for wheat and rye and to ensure the availability of bread [3]. When ...
... production of beer were water, barley, and hops. The principal purpose of the law was to prevent of beer were water, barley, and hops. The principal purpose of the law was to prevent price competition price competition with bakers for wheat and rye and to ensure the availability of bread [3]. When ...
DOCX format - 76 KB - Office of the Gene Technology Regulator
... More information about the decision to issue this licence is contained in the Risk Assessment and Risk Management Plan prepared in connection with the assessment of the application for the licence. This document can be obtained from the Office of the Gene Technology Regulator website, or by telephon ...
... More information about the decision to issue this licence is contained in the Risk Assessment and Risk Management Plan prepared in connection with the assessment of the application for the licence. This document can be obtained from the Office of the Gene Technology Regulator website, or by telephon ...
Assessing the role of anammox in a nitrogen contaminated aquifer
... Several decades before the discovery of anammox bacteria, Austrian theoretical chemist Engelbert Broda (1977) put forth the idea that organisms could be capable of oxidizing ammonium by using nitrite or nitrate as an electron acceptor. Richards (1965) even demonstrated the mysterious and unexplainab ...
... Several decades before the discovery of anammox bacteria, Austrian theoretical chemist Engelbert Broda (1977) put forth the idea that organisms could be capable of oxidizing ammonium by using nitrite or nitrate as an electron acceptor. Richards (1965) even demonstrated the mysterious and unexplainab ...
Functional characterization of the Mycobacterium
... (Invitrogen) were transformed with the resulting plasmids and the purified recombinant protein was used for the PepChip array kinase assay only. In parallel, an 807 bp PCR product of pknJ amplified with primers pMALpknJFd and pMALpknJRv (Table S2) was ligated into vector pMAL-c2X (New England Biolab ...
... (Invitrogen) were transformed with the resulting plasmids and the purified recombinant protein was used for the PepChip array kinase assay only. In parallel, an 807 bp PCR product of pknJ amplified with primers pMALpknJFd and pMALpknJRv (Table S2) was ligated into vector pMAL-c2X (New England Biolab ...
Involvement of Polyamine Catabolism in the Regulation of Glucose
... regulation of glucose, energy and lipid metabolism was investigated using transgenic mice overexpressing or deficient for SSAT. The characterization of SSAT mice revealed that these mice exhibited severely reduced whole body WAT mass, elevated energy expenditure, high insulin sensitivity, a low tiss ...
... regulation of glucose, energy and lipid metabolism was investigated using transgenic mice overexpressing or deficient for SSAT. The characterization of SSAT mice revealed that these mice exhibited severely reduced whole body WAT mass, elevated energy expenditure, high insulin sensitivity, a low tiss ...
Document
... Mice and humans share virtually the same set of genes Mice are well-established experimental model. Genes easily found in mice genome sequences Test experimentally the function of those genes. ...
... Mice and humans share virtually the same set of genes Mice are well-established experimental model. Genes easily found in mice genome sequences Test experimentally the function of those genes. ...
Mutants of the Drosophila ncd microtubule motor
... and Church, 1983) has led to the idea that the primary defect is the oocyte microtubule organizing center (Kimble and Church, 1983). Localization of ncd to meiotic spindles from Drosophila oocytes (Hatsumi and Endow, 1992a) and the minus-end polarity of ncd movement on microtubules resulted in the p ...
... and Church, 1983) has led to the idea that the primary defect is the oocyte microtubule organizing center (Kimble and Church, 1983). Localization of ncd to meiotic spindles from Drosophila oocytes (Hatsumi and Endow, 1992a) and the minus-end polarity of ncd movement on microtubules resulted in the p ...
Glutamine and the bowel
... → intestinal glutamine metabolism： 1. Contribute a nutritionally important portion of intestinal energy generation. 2. As precursor：synthesis of ornithine, cotrulline, proline and arginine. ...
... → intestinal glutamine metabolism： 1. Contribute a nutritionally important portion of intestinal energy generation. 2. As precursor：synthesis of ornithine, cotrulline, proline and arginine. ...
The-NOS-problem
... Caveats: constitutive expression of NOS cDNA might not mimic the spatially and temporally controlled endogenous expression of NOS; this might be a reason it doesn’t rescue. Pat says that since the expression of NOS cDNA doesn’t even modify the stage of lethality, this shows that the lethality must b ...
... Caveats: constitutive expression of NOS cDNA might not mimic the spatially and temporally controlled endogenous expression of NOS; this might be a reason it doesn’t rescue. Pat says that since the expression of NOS cDNA doesn’t even modify the stage of lethality, this shows that the lethality must b ...
Systems biology of inborn errors of metabolism
... leading to life threatening conditions when left un-treated. IEMs not only demand better diagnostic methods and efficient therapeutic regimen, but also, a high level understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the cor ...
... leading to life threatening conditions when left un-treated. IEMs not only demand better diagnostic methods and efficient therapeutic regimen, but also, a high level understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the cor ...
Metal Complexes Containing Natural and Artificial Radioactive
... member of the actinide series of elements, although it has no 5f electrons in its metallic, gaseous, or ionic forms [1]. Technetium (element 43) possesses two radioactive isotopes with long half-lives: 99Tc (2.12 × 105 years, has practical use) and 98Tc (1.5 × 106 years, it is a rhenium analogue). B ...
... member of the actinide series of elements, although it has no 5f electrons in its metallic, gaseous, or ionic forms [1]. Technetium (element 43) possesses two radioactive isotopes with long half-lives: 99Tc (2.12 × 105 years, has practical use) and 98Tc (1.5 × 106 years, it is a rhenium analogue). B ...