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Disorders of mitochondrial function
Disorders of mitochondrial function

Modern Materials: Polymers and Biomaterials
Modern Materials: Polymers and Biomaterials

... Plastic: materials that can be formed into shapes. Thermoplastic: materials that can be shaped more than once.  (Used as replacements for blood vessels.) Thermosetting: materials that can only be shaped once (Used in  dental devices, and orthopedics such as hip replacements.) Elastomer: material tha ...
PerfectBabyFullText
PerfectBabyFullText

... to the philosophical method developed by William James and John Dewey, in the first booklength attempt to use classical American pragmatism to solve a problem in bioethics. It is an important method, one that brings the importance of empirical investigation and scientific discovery to bear on moral ...
Disorders of mitochondrial function
Disorders of mitochondrial function

... of mutant mtDNA; this threshold presumably depends on the energy requirement of each tissue. Most pediatric mitochondrial diseases are caused by defects of proteins encoded by nuclear genes that are transported into mitochondria [2]. These mitochondrial diseases are inherited in Mendelian fashion. ...
Genetic code as a harmonic system
Genetic code as a harmonic system

... Figure 1 (left). The distribution of amino acids according to Gauss’ algorithm; (a) the distribution of amino acids has been derived from Table 1.1, by presenting two pairs here in one row as the “pair of pairs”. In the beginning of each row obtained in this way one “contact” amino acid has been as ...
Short-Term Overexpression of a Constitutively Active Form of AMP
Short-Term Overexpression of a Constitutively Active Form of AMP

... of primers (Table 1), 10 ␮l of 2 ⫻ PCR mix, and 0.5 ␮l of Sybr Green. The reactions were carried out in capillaries in a LightCycler instrument (Roche) with 40 cycles. We determined the relative amounts of the mRNAs studied by means of the second-derivative maximum method, with LightCycler analysis ...
Characteristics of Phenylacrylic Acid Decarboxylase
Characteristics of Phenylacrylic Acid Decarboxylase

... selective  functionalization  of  nonactivated  groups  in  organic  molecules4.     The   repertoire   of   enzymatic   chemistry   is   only   expected   to   increase   due   to   the   large   amount   of   genomic   data   that   is   becoming   available.   A   recent   publication   described ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
Osmo- and thermo-adaptation in hyperthermophilic Archaea

... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
Chapter 2 Biological Beginnings CHAPTER 2: BIOLOGICAL
Chapter 2 Biological Beginnings CHAPTER 2: BIOLOGICAL

... whole or part. ...
Gregor Mendel was a 19th century priest and botanist who
Gregor Mendel was a 19th century priest and botanist who

... He articulated the laws of inheritance attributed to him insofar as was possible given the information he had.” -- [Fairbanks and Rytting (2001)]. Did Mendel detect but not mention linkage? o Mendel concluded "the behavior of each pair of differing traits in a hybrid association is independent of al ...
Life in the slow lane: molecular mechanisms of estivation
Life in the slow lane: molecular mechanisms of estivation

... 1997). Large changes to the activity state of many enzymes and functional proteins can be made via the addition or removal of covalently bound phosphate through the action of protein kinases and protein phosphatases. The virtually onyoff control of glycogen phosphorylase (GP) that is provided by thi ...
Common Quantitative Trait Loci for Alcohol
Common Quantitative Trait Loci for Alcohol

... degrees of freedom for those strains. Therefore, in table 1, we have included the correlations between NTR densities and ST as well as between all NT measures and BECRR and hypothermia. Among the LSXSS RI strains, ST and BECRR are negatively correlated measures of hypnotic sensitivity (DeFries et al ...
Method To Develop High Oleic Acid Soybeans Using Conventional
Method To Develop High Oleic Acid Soybeans Using Conventional

... [0015] While transgenic soybean lines have been generated that produce soybean oil containing mid to high levels of oleic ...
The alternative splicing of tau exon10 and its
The alternative splicing of tau exon10 and its

Jesse Rabinowitz - National Academy of Sciences
Jesse Rabinowitz - National Academy of Sciences

... Relatively large amounts of ferredoxin were found in four different Clostridial strains tested (Buchanan, Lovenberg, and Rabinowitz 1963). Furthermore, ferredoxin presence was demonstrated in obligately anaerobic bacteria, but not in aerobic or facultatively anaerobic organisms. The ferredoxins from ...
Genetic Characterisation of Human ABO Blood
Genetic Characterisation of Human ABO Blood

... In 1628, British physician W Harvey described the circulation of the blood in humans after having pumped water through the blood vessels of a corpse (Harvey, 1628). This observation opened a new era and some decades later, in 1667, J-B Denis (Denis, 1667) in France and R Lower (Lower, 1666) in Engla ...
The Homothorax homeoprotein activates the nuclear localization of
The Homothorax homeoprotein activates the nuclear localization of

Recent advances in biosynthesis of fatty acids derived products in
Recent advances in biosynthesis of fatty acids derived products in

... The activation of acetate is so ATP intensive that it constrains the maximal yield of the acetyl-CoA derived products. In addition, the supply of acetyl-CoA is even more limited under anaerobic conditions, a preferred process for industrial applications. Many prokaryotes contain an acetylating aceta ...
The Effect of a Limited Supply of Phenylalanine, Threonine, or
The Effect of a Limited Supply of Phenylalanine, Threonine, or

... Lys and Val; these are extracted by the mammary secretory cells from the blood in excess relative to the amount secreted in milk protein. These excess EAA might be used as a potential energy precursor or as a source of carbon (C) and N for synthesis of other components, especially NEAA and fatty aci ...
Analysis of the Molecular Basis of Flowering Time Variation in
Analysis of the Molecular Basis of Flowering Time Variation in

Ethylene Signal Transduction. Moving beyond
Ethylene Signal Transduction. Moving beyond

Insulin-like growth factor (IGF) signalling is required for early dorso
Insulin-like growth factor (IGF) signalling is required for early dorso

... These findings suggest that neural induction in the Xenopus embryo is favoured by low Smad1 activity and high MAPK signalling (Pera et al., 2003). A study in cultured cells indicates that IGF signalling pathways in zebrafish are similar to those in mammalian systems and that IGF-1 binds to the IGF-1 ...
Protein Structure, Neighbor Effect, and a New Index of Amino Acid
Protein Structure, Neighbor Effect, and a New Index of Amino Acid

... The arbitrary choice of amino acid properties and potentially false formulation of the amino acid dissimilarities may be responsible for some of the old controversies between Kimura (1983, p. 159) and Gillespie (1991, p. 43). Kimura, being a neutralist, argued that the most frequent nonsynonymous su ...
Computational Identification of Plant MicroRNAs and
Computational Identification of Plant MicroRNAs and

Potassium starvation responses in yeast highlight novel potassium-related functions
Potassium starvation responses in yeast highlight novel potassium-related functions

... diameter and 70 fL of volume when growing in rich complete medium. In this situation, the composition of haploid yeast cells is 0.017 pg of DNA, 1.2 pg of RNA and 6 pg of proteins (Sherman 2002). The yeast genome is composed by 12.1 Mbp arranged across 16 chromosomes encoding around of 6000 genes of ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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