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New Host Plants of Erwinia amylovora in Bulgaria
New Host Plants of Erwinia amylovora in Bulgaria

... lovora. The amplified product was of 519 bp and corresponded to preliminary sequential analysis on GenBank data. It was shown that E. pyrifoliae gave also a positive reaction. The molecular characterization of E. pyrifoliae demonstrated substantial homology with the ams-genes of E. amylovora. The pa ...
Genetic Characterization and Linkage Mapping of
Genetic Characterization and Linkage Mapping of

Euglena gracilis ascorbate peroxidase forms an intramolecular
Euglena gracilis ascorbate peroxidase forms an intramolecular

... adopted a PCR-based oligo-capped method utilizing primers corresponding to sequences of an EST clone and a partial cDNA isolated previously [19]. The final clone containing the full-length cDNA, as indicated by the presence of a 5 -TTTTTTTTCG-3 spliced leader at the 5 end [20], was 2403 bp long w ...
Fitness landscapes of gene regulation in variable - UvA-DARE
Fitness landscapes of gene regulation in variable - UvA-DARE

... the predicted optimum. In a number of instances adaptation occurred, but the predicted optimum was not reached. This may be due to a diminishing return of further optimization, or to the existence of a functional or genetic constraint. ...
hemipterous Encodes a Novel Drosophila MAP
hemipterous Encodes a Novel Drosophila MAP

A survey on computational methods for enhancer and
A survey on computational methods for enhancer and

Dissolving Dominance
Dissolving Dominance

... wholly novel in this claim or approach. Mendel himself cites work by Gärtner, and there were others earlier in the century (Orel 1996). In this respect, Mendel's concept of dominant traits would have been important, but hardly revolutionary (and hence not especially noteworthy to his contemporaries) ...
hirota - Genetics
hirota - Genetics

... containing these mutations were pili+ and others were pili-. That such phenotypically similar mutants were obtained as deletion mutations may suggest the presence of subunits in cistron C which probably control piliation. The pili+ mutants having deletions for all of cistron A and at the same time, ...
The Major Component of the Paraflagellar Rod of Trypanosoma
The Major Component of the Paraflagellar Rod of Trypanosoma

... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
Novel Allergen Structures with Tandem Amino Acid Repeats
Novel Allergen Structures with Tandem Amino Acid Repeats

... patients and are B. germanica-specific (12). Antigenically cross-reactive allergens have been identified in both German and American cockroach (Bla g 1 and Per a 1) but their structure was unknown (13–15). We recently cloned the cDNA encoding Per a 1 and here we report the sequences of three Bla g 1 ...
A re-evaluation of the ATP :NADPH budget
A re-evaluation of the ATP :NADPH budget

... aconitase and NADP-ICDH (4b; Chen and Gadal, 1990). For pyruvate production via NAD-ME, it is assumed that the high-affinity mitochondrial OAA transporter ( Ebbighausen et al., 1985) competes effectively with cytosolic MDH so that OAA is transported into the mitochondrion before being reduced to mal ...
Reproductive barriers in indica-japonica rice
Reproductive barriers in indica-japonica rice

... transmembrane protein ORF4+, which induces ER stress and subsequently leading to ...
Effete, a Drosophila chromatin-associated ubiquitin
Effete, a Drosophila chromatin-associated ubiquitin

... Subsequent studies showed that these variegating white+ transgenes were inserted within or next to a cluster of subtelomeric repeats, designed as Telomere Associated Sequences (TAS) (Karpen and Spradling 1992; Levis et al. 1993; Cryderman et al. 1999; Mason et al. 2000; Golubovsky et al. 2001). TAS ...
Vitamins: Biochemical Roles
Vitamins: Biochemical Roles

... function most often as coenzymes. • Humans must consume at least 12 vitamins in their diet, because we lack the ability to synthesize them. • A well-balanced diet from a variety of food sources usually provides all these vitamins. However, many people supplement their diet with extra vitamins. • Mos ...
thèse - Université Evry Val d`Essonne
thèse - Université Evry Val d`Essonne

... proteins. mtDNA generally encodes catalytic subunits of the complexes of the respiratory chain with tRNA and rRNA components of the mitochondrial translation system, but it can also contain additional proteins; in S. cerevisiae, besides seven subunits of complexes involved in oxidative phosphorylati ...
Vitamin B12
Vitamin B12

... • Individuals who lack intrinsic factor have a decreased ability to absorb B12. • Gastric acid releases the vitamin from food particles: – Therefore antacid and acid-blocking medications may inhibit absorption of B12. • In addition some elderly people produce less stomach acid as they age thereby in ...
A novel role for the floral homeotic gene APETALA2
A novel role for the floral homeotic gene APETALA2

... valves and replum, respectively. In ful rpl double mutants, the valve margin identity genes become ectopically expressed, and, as a result, the entire outer surface of the ovary takes on valve margin identity. We carried out a genetic screen in this sensitized genetic background and identified a sup ...
Mutations at the Darkener of apricot Locus Modulate Transcript
Mutations at the Darkener of apricot Locus Modulate Transcript

... transposon distinct from copia (ZACHARand BINCHAM 1982). T h e Doa product presumably interacts with sequences contained within the transposons inserted at wa and w5Ps5,since white alleles with point mutations do not respond (RABINOW and BIRCHLER 1989). Doa acts upon wa as an inverse function ofits ...
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells

... (denoted by the thick downwards-pointing arrow in Figure 1), because it yields only 2 ATPs per molecule of glucose via ‘substrate-level phosphorylation’ of ADP. S. cerevisiae is one of the few organisms that prefers to ferment glucose, even when oxygen is abundant. The resulting low yield of ATP pro ...
9th Grade Physical Science Course Syllabus
9th Grade Physical Science Course Syllabus

... Objectives: Students should be able to: Define the term atomic mass. Calculate the number of neutrons in an atom. Identify the elements most common in organisms. Identify covalent and ionic bonds. Describe the nature of polar covalent and nonpolar covalent bonds. Explain the importance of hydrogen b ...
14steriod
14steriod

... - Bile consist of a watery mixture of organic and inorganic compounds. Bile can go from the liver directly to the intestine or to be stored at bilary gland. - The most common bile acid : Cholic and Chenodeoxycholic acid. - Bile acids are not fully ionized at pH = 7 because they have pKa = 6, (they a ...
S17 Cholesterol And Steroids Biosynthesis
S17 Cholesterol And Steroids Biosynthesis

... - Bile consist of a watery mixture of organic and inorganic compounds. Bile can go from the liver directly to the intestine or to be stored at bilary gland. - The most common bile acid : Cholic and Chenodeoxycholic acid. - Bile acids are not fully ionized at pH = 7 because they have pKa = 6, (they a ...
Fig. - DIGITAL.CSIC, el repositorio institucional
Fig. - DIGITAL.CSIC, el repositorio institucional

Quality control in tRNA charging — editing of homocysteine
Quality control in tRNA charging — editing of homocysteine

... tRNA is achieved by preferential binding, which is facilitated by structural variation between tRNAs (Schulman, 1991). In contrast, the structural variation between amino acids is not sufficient and an AARS often selects two or more similar amino acids (Baldwin & Berg, 1966). In those cases, faithfu ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis

... (O2-), hydroxyl radical (HO) and hydrogen peroxide (H2O2), all of which are normal byproducts of oxygen metabolism. Oxygen has a unique molecular structure with unique electron configuration resulting from two unpaired electrons in ground state of O2 molecule (triplet oxygen). It readily accepts el ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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