Improving penicillin biosynthesis in Penicillium chrysogenum
... random mutagenesis and selection procedures have been successful in generating a strain lineage that shows enhanced PEN production levels. More recently, also genetic engineering was introduced in strain improvement programs. These efforts have primarily focused on increasing the levels of (or intro ...
... random mutagenesis and selection procedures have been successful in generating a strain lineage that shows enhanced PEN production levels. More recently, also genetic engineering was introduced in strain improvement programs. These efforts have primarily focused on increasing the levels of (or intro ...
PSI-BLAST is used for
... I. Friedberg and H. Margalit: Persistently Conserved Positions inStructurally-Similar, Sequence Dissimilar Proteins: Roles in PreservingProtein Fold and Function (2002) Protein Science 11(2):350-360 ...
... I. Friedberg and H. Margalit: Persistently Conserved Positions inStructurally-Similar, Sequence Dissimilar Proteins: Roles in PreservingProtein Fold and Function (2002) Protein Science 11(2):350-360 ...
GuanHongLi (275
... antihypertensive, opioid, immunomodulating, antioxidative, antimicrobial, antithrombotic, antiamnestic, hypocholesterolemic and other activities. 3,14,15 These peptides are inactive within the sequence of parent proteins, but they can be released by enzymatic proteolysis in vivo or in vitro, for exa ...
... antihypertensive, opioid, immunomodulating, antioxidative, antimicrobial, antithrombotic, antiamnestic, hypocholesterolemic and other activities. 3,14,15 These peptides are inactive within the sequence of parent proteins, but they can be released by enzymatic proteolysis in vivo or in vitro, for exa ...
Intrinsically Disordered Protein - Center for Data Analytics and
... alternative 3D structures in multisubunit proteins. Several models have been developed to explain this behavior, two of which are symmetric allosterism, by Monod, Wyman and Changeux26 and sequential allosterism, by Koshland, Nemethy and Filman.27 Discovery of intrinsically disordered protein segment ...
... alternative 3D structures in multisubunit proteins. Several models have been developed to explain this behavior, two of which are symmetric allosterism, by Monod, Wyman and Changeux26 and sequential allosterism, by Koshland, Nemethy and Filman.27 Discovery of intrinsically disordered protein segment ...
Amino Acid Sequences and Evolutionary Relationships
... biologists have found that such biochemical evidence compares favorably with other lines of evidence for evolutionary relationships. An interesting additional line of evidence supporting evolution involves sequences of DNA known as "pseudogenes." Pseudogenes are remnants of genes that no longer func ...
... biologists have found that such biochemical evidence compares favorably with other lines of evidence for evolutionary relationships. An interesting additional line of evidence supporting evolution involves sequences of DNA known as "pseudogenes." Pseudogenes are remnants of genes that no longer func ...
The role of carbohydrate in sperm
... affinities for their substrates, e.g. the arabinose binding protein has a K
... affinities for their substrates, e.g. the arabinose binding protein has a K
NSPKU
... But it contains all of the other amino acids in protein that your child needs. • Most protein substitutes also contain vitamins, minerals and other important nutrients to ensure your child is getting what they need. The protein substitute is an extremely important part of the diet for a person with ...
... But it contains all of the other amino acids in protein that your child needs. • Most protein substitutes also contain vitamins, minerals and other important nutrients to ensure your child is getting what they need. The protein substitute is an extremely important part of the diet for a person with ...
the Gene Ontology
... 1. Does it include my species of interest (or do I have to “humanize” my list)? 2. What does it require to set up (computer usage/online) 3. What was the source for the GO (primary or secondary) and when was it last updated? 4. Does it report the GO evidence codes (and is IEA included)? 5. Does it r ...
... 1. Does it include my species of interest (or do I have to “humanize” my list)? 2. What does it require to set up (computer usage/online) 3. What was the source for the GO (primary or secondary) and when was it last updated? 4. Does it report the GO evidence codes (and is IEA included)? 5. Does it r ...
Capabilities and limitations of gel electrophoresis for elemental
... because of its excellent limits of detection, but also because it offers truly quantitative results. The availability of a real blank, the gel itself, is an important advantage. With it, a real procedural limit of detection (LoD) can, for example, be determined. The blank model can even be further r ...
... because of its excellent limits of detection, but also because it offers truly quantitative results. The availability of a real blank, the gel itself, is an important advantage. With it, a real procedural limit of detection (LoD) can, for example, be determined. The blank model can even be further r ...
1 Engineering Lipases with an Expanded Genetic Code - Wiley-VCH
... of orthogonal aminoacyl-tRNA synthetase/tRNA pairs, which install ncAAs at amber stop codon inserted into the protein sequence, a methodology called stop codon suppression (SCS) [27, 28]. The global replacement and site-specific technologies, of course, can also be combined. Hoesl and Budisa [29] sho ...
... of orthogonal aminoacyl-tRNA synthetase/tRNA pairs, which install ncAAs at amber stop codon inserted into the protein sequence, a methodology called stop codon suppression (SCS) [27, 28]. The global replacement and site-specific technologies, of course, can also be combined. Hoesl and Budisa [29] sho ...
Identification, Expression and Characterization of Archaeal
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
Discovery of Paralogous Nuclear Gene Sequences Coding for the
... 1999). Multiple copies of RPB1 have been reported in Glycine max (Dietrich, Prenger, and Guilfoyle 1990), but these multiple copies may be due to the polyploid nature of the soybean genome (Kumar and Hymowitz 1989; Denton, McConaughy, and Hall 1998). RPB2 encodes the second-largest subunit of nuclea ...
... 1999). Multiple copies of RPB1 have been reported in Glycine max (Dietrich, Prenger, and Guilfoyle 1990), but these multiple copies may be due to the polyploid nature of the soybean genome (Kumar and Hymowitz 1989; Denton, McConaughy, and Hall 1998). RPB2 encodes the second-largest subunit of nuclea ...
Supporting Information
... The two structures superimpose with an rmsd of 2.5 Å over 443 matched C␣ positions. The most notable differences are found in the helical cap (top) of the propeller and in blade III, where RPE65 contains a ⬇30 residue extension. The dashed maroon line indicates the approximate position the disordere ...
... The two structures superimpose with an rmsd of 2.5 Å over 443 matched C␣ positions. The most notable differences are found in the helical cap (top) of the propeller and in blade III, where RPE65 contains a ⬇30 residue extension. The dashed maroon line indicates the approximate position the disordere ...
Protein Structure Prediction with Lattice Models
... Exhaustive search of a protein’s conformational space is clearly not a feasible algorithmic strategy. The number of possible conformations is exponential in the length of the protein sequence, and powerful computational hardware would not be capable of searching this space for even moderately large ...
... Exhaustive search of a protein’s conformational space is clearly not a feasible algorithmic strategy. The number of possible conformations is exponential in the length of the protein sequence, and powerful computational hardware would not be capable of searching this space for even moderately large ...
AMINO ACID SEQUENCES AND EVOLUTIONARY RELATIONSHIPS
... proteins of similar and diverse animals, evolutionary relationships that might otherwise go undetected, can be determined. Biologists believe that the greater the similarity between the amino acid sequences of two organisms, the closer the evolutionary relationship. In other words, the more recently ...
... proteins of similar and diverse animals, evolutionary relationships that might otherwise go undetected, can be determined. Biologists believe that the greater the similarity between the amino acid sequences of two organisms, the closer the evolutionary relationship. In other words, the more recently ...
THE INFLUENCE OF BODY MASS INDEX (BMI)
... needs. Circumstances can cause a non-essential amino acid to become essential if the body can no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought ...
... needs. Circumstances can cause a non-essential amino acid to become essential if the body can no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought ...
Free tyrosine and tyrosine-rich peptide
... Previously, generation of superoxide anion (O2•-) catalyzed by Cu-binding peptides derived from human prion protein (model sequence for helical Cu-binding motif VNITKQHTVTTTT was most active) in the presence of catecholamines and related aromatic monoamines such as phenylethylamine and tyramine, has ...
... Previously, generation of superoxide anion (O2•-) catalyzed by Cu-binding peptides derived from human prion protein (model sequence for helical Cu-binding motif VNITKQHTVTTTT was most active) in the presence of catecholamines and related aromatic monoamines such as phenylethylamine and tyramine, has ...
translation and protein structure
... to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is also the reason why most hydrophobic amino acids tend to be buried ...
... to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is also the reason why most hydrophobic amino acids tend to be buried ...
sequence-structure relationship - HAL
... nuclear magnetic resonance analysis are difficult to undertake. Prediction methods are based on the fact that the amino acid sequence of a protein specifies its 3D structure. Different approaches are currently used to predict global protein structures: (i) comparative modeling is carried out when th ...
... nuclear magnetic resonance analysis are difficult to undertake. Prediction methods are based on the fact that the amino acid sequence of a protein specifies its 3D structure. Different approaches are currently used to predict global protein structures: (i) comparative modeling is carried out when th ...
Relationship between Hot Spot Residues and Ligand Binding Hot
... complexes listed in Table 1, which lists their PDB codes, names, receptor/ligand role, and the source for alanine scanning data. The complexes are either enzymes with inhibiting proteins, or signal modulating protein complexes where it has been shown that the binding of the partner protein can be re ...
... complexes listed in Table 1, which lists their PDB codes, names, receptor/ligand role, and the source for alanine scanning data. The complexes are either enzymes with inhibiting proteins, or signal modulating protein complexes where it has been shown that the binding of the partner protein can be re ...
Amiito acid sequence of the testosterone
... single gene (2,10). Previous work has reported two different polyadenylatIon sites that are recognized to produce the 1450 and 1950 nt mRNAs in the kidney (9). In this paper we identify a third polyadenylation site, responsible for the generation of the 1350 nt species. The hormonal regulation and t ...
... single gene (2,10). Previous work has reported two different polyadenylatIon sites that are recognized to produce the 1450 and 1950 nt mRNAs in the kidney (9). In this paper we identify a third polyadenylation site, responsible for the generation of the 1350 nt species. The hormonal regulation and t ...
Biomolecular chemistry 3. Translating the genetic code
... just one codon each. The other 18 amino acids are each encoded by two or more. Indeed, leucine (Leu), arginine (Arg), and serine (Ser) are specified by six codons each. Codons that specify the same amino acid are called synonyms. For example, CAU and CAC are synonyms for histidine. Note that synonym ...
... just one codon each. The other 18 amino acids are each encoded by two or more. Indeed, leucine (Leu), arginine (Arg), and serine (Ser) are specified by six codons each. Codons that specify the same amino acid are called synonyms. For example, CAU and CAC are synonyms for histidine. Note that synonym ...
The Computational Complexity of Protein
... Computational robustness refers to the independence of algorithmic results from particular settings. In the context of NP-completeness, robustness refers to the fact that a class of closely related problems can be described, all of which are NP-complete. The members of the class of problems are typi ...
... Computational robustness refers to the independence of algorithmic results from particular settings. In the context of NP-completeness, robustness refers to the fact that a class of closely related problems can be described, all of which are NP-complete. The members of the class of problems are typi ...
calculating the structure-based phylogenetic relationship
... utilizing polypeptide sequences. However, because protein structures superiorly conserve homology and contain more biochemical information than their associated protein sequences, I hypothesize that utilizing the structure of a protein instead of its sequence will generate a superior dendrogram. Gen ...
... utilizing polypeptide sequences. However, because protein structures superiorly conserve homology and contain more biochemical information than their associated protein sequences, I hypothesize that utilizing the structure of a protein instead of its sequence will generate a superior dendrogram. Gen ...
Proceedings of the Nutrition Society Safety and efficacy of high
... glycaemic-index (GI) components. Specifically, of the five popular diet types tested, a HP, low GI diet provided the greatest opportunity for WL maintenance. The dropout rate was lower in the groups that were assigned to HP diets and the groups that were assigned to low-GI diets than in the group th ...
... glycaemic-index (GI) components. Specifically, of the five popular diet types tested, a HP, low GI diet provided the greatest opportunity for WL maintenance. The dropout rate was lower in the groups that were assigned to HP diets and the groups that were assigned to low-GI diets than in the group th ...