Nutrient Utilization in Swine
... essential amino acid, and formation of protein ceases even though the other essential amino acids are available. In typical swine corn-soybean meal diets, the first limiting essential amino acid is lysine, which explains why lysine is often added to swine diets. Animals become fat when the body does ...
... essential amino acid, and formation of protein ceases even though the other essential amino acids are available. In typical swine corn-soybean meal diets, the first limiting essential amino acid is lysine, which explains why lysine is often added to swine diets. Animals become fat when the body does ...
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin
... intracellular dissolved oxygen [44]. Nowadays, it has become a versatile tool in current biotechnology, especially for large-scale fermentation, since shortage of oxygen supply during cultivation can be overcome by heterologous expression of VHb. Cell growth and productivity are enhanced particularl ...
... intracellular dissolved oxygen [44]. Nowadays, it has become a versatile tool in current biotechnology, especially for large-scale fermentation, since shortage of oxygen supply during cultivation can be overcome by heterologous expression of VHb. Cell growth and productivity are enhanced particularl ...
The first true obligately syntrophic propionate
... fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were characterized by sequence analysis of the insert DNA using t ...
... fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were characterized by sequence analysis of the insert DNA using t ...
PLANT PROTEIN PHOSPHATASES
... Therefore, isoforms that harbor the phosphorylation recognition site may be selectively targeted for inactivation by cdc2-like protein kinases. The phosphorylation site is found at a conserved position in about half of the known plant PP1 catalytic subunits (116), but whether they are subject to thi ...
... Therefore, isoforms that harbor the phosphorylation recognition site may be selectively targeted for inactivation by cdc2-like protein kinases. The phosphorylation site is found at a conserved position in about half of the known plant PP1 catalytic subunits (116), but whether they are subject to thi ...
The first true obligately syntrophic propionate
... fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were characterized by sequence analysis of the insert DNA using t ...
... fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were characterized by sequence analysis of the insert DNA using t ...
WebMOTIFS: Web-based integrated motif discovery
... Program Description and Organization WebMOTIFS is designed to automate the identification of regulatory sequence motifs using multiple motif discovery algorithms. Users may provide gene names (RefSeq or yeast ORF names) or probe identifiers from one of several microarray platforms for S. cerevisiae ...
... Program Description and Organization WebMOTIFS is designed to automate the identification of regulatory sequence motifs using multiple motif discovery algorithms. Users may provide gene names (RefSeq or yeast ORF names) or probe identifiers from one of several microarray platforms for S. cerevisiae ...
Processing of the Presequence of the Schizosaccharomyces pombe
... the S. pombe iron-sulfur protein into S. cerevisiae mitochondria (Fig. 3B). Again only precursor and mature length protein is detectable, suggesting that the three amino acid motif is not sufficient by itself for sequential two-step processing. To test whether S. pombe mitochondria contain any MPP a ...
... the S. pombe iron-sulfur protein into S. cerevisiae mitochondria (Fig. 3B). Again only precursor and mature length protein is detectable, suggesting that the three amino acid motif is not sufficient by itself for sequential two-step processing. To test whether S. pombe mitochondria contain any MPP a ...
The proteome of Saccharomyces cerevisiae mitochondria
... The most striking effect was observed, however, when the dependence on the inner membrane potential ⌬ was determined. At least five of the proteins were specifically processed by mitochondria only in the presence of a ⌬ (Fig. 3A): Lap3, an aminopeptidase of the cysteine protease family (38, 39); N ...
... The most striking effect was observed, however, when the dependence on the inner membrane potential ⌬ was determined. At least five of the proteins were specifically processed by mitochondria only in the presence of a ⌬ (Fig. 3A): Lap3, an aminopeptidase of the cysteine protease family (38, 39); N ...
A standard nomenclature for von Willebrand factor gene mutations
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
Prokaryotic features of a nucleus
... two independent three-dimensional units, which were highly conserved during the divergence of prokaryotes and eukaryotes: the coenzyme-binding domain, which has a similar structure in all NAD-dependent dehydrogenases, and the catalytic domain, which is specific for GAPDH and which differs in differe ...
... two independent three-dimensional units, which were highly conserved during the divergence of prokaryotes and eukaryotes: the coenzyme-binding domain, which has a similar structure in all NAD-dependent dehydrogenases, and the catalytic domain, which is specific for GAPDH and which differs in differe ...
Supplementary Figures 1 - 5, Methods
... The expression of Globo-H on (A) MCF-7 cells and (B) MDA-MB-157 cells was determined by flow cytometry with mAbVK9. (C) Microvesicles (MVs, 50g) harvested from the supernatants of MDA-MB-157 cells (MB-MVs) were incubated with HUVECs for 4h and examined for surface Globo-H. (D) Serial dilutions of M ...
... The expression of Globo-H on (A) MCF-7 cells and (B) MDA-MB-157 cells was determined by flow cytometry with mAbVK9. (C) Microvesicles (MVs, 50g) harvested from the supernatants of MDA-MB-157 cells (MB-MVs) were incubated with HUVECs for 4h and examined for surface Globo-H. (D) Serial dilutions of M ...
Genomics Meets Phylogenetics
... By preserving the information in individual amino acid or nucleic acid states, parsimony methods make possible a detailed examination of the processes by which molecular characters evolved and brought about novel aspects of protein function. Second, phenetic methods have a tendency to create a false ...
... By preserving the information in individual amino acid or nucleic acid states, parsimony methods make possible a detailed examination of the processes by which molecular characters evolved and brought about novel aspects of protein function. Second, phenetic methods have a tendency to create a false ...
A speculation on the origin of protein synthesis
... errors. On the other hand an occasional incorrect amino acid will not necessarily be unacceptable. It seems likely that one such requirement is that, at any moment, the particular tRNA molecule to which the growing polypeptide chain is attached is bound to the messenger RNA by sufficiently strong bo ...
... errors. On the other hand an occasional incorrect amino acid will not necessarily be unacceptable. It seems likely that one such requirement is that, at any moment, the particular tRNA molecule to which the growing polypeptide chain is attached is bound to the messenger RNA by sufficiently strong bo ...
Colicins produced by the Escherichia fergusonii strains closely
... backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were ...
... backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were ...
CWS/5/7 Rev.1 ADD (in Spanish)
... case, the priority application and sequence listing are themselves vague. Care should be taken to draft the original (ST.25) sequence listing and application disclosure to include the unabbreviated name to avoid future issues. To avoid potential deleted matter, the abbreviated name from the ST.25 se ...
... case, the priority application and sequence listing are themselves vague. Care should be taken to draft the original (ST.25) sequence listing and application disclosure to include the unabbreviated name to avoid future issues. To avoid potential deleted matter, the abbreviated name from the ST.25 se ...
Function and biotechnology of extremophilic enzymes in low water
... X-ray and neutron diffraction structures have confirmed that the high content of acidic residues play significant roles in binding of essential water molecules and salt ions, preventing protein aggregation and providing flexibility to protein structure through electrostatic repulsion (Figure 3). For ...
... X-ray and neutron diffraction structures have confirmed that the high content of acidic residues play significant roles in binding of essential water molecules and salt ions, preventing protein aggregation and providing flexibility to protein structure through electrostatic repulsion (Figure 3). For ...
Identification of sixteen single-nucleotide polymorphism markers in
... (AS-PCR) (Newton et al. 1989), is more flexible with a reasonable efficiency and expense (Huang et al. 2005; Gaudet et al. 2007), and has also been applied the identification of SNP markers in mollusks, such as Meretrix meretrix (Li et al. 2010), Crassostrea gigas (Wang et al. 2010) and Mytilus gall ...
... (AS-PCR) (Newton et al. 1989), is more flexible with a reasonable efficiency and expense (Huang et al. 2005; Gaudet et al. 2007), and has also been applied the identification of SNP markers in mollusks, such as Meretrix meretrix (Li et al. 2010), Crassostrea gigas (Wang et al. 2010) and Mytilus gall ...
Nutrition to Support Recovery from Endurance Exercise: Optimal
... on carbohydrate as a source of fuel (40,45). In the face of enhanced glucose uptake by working muscles, blood glucose levels are maintained to a large extent by the breakdown of liver glycogen stores. Despite this enhanced liver glycogenolysis, muscle glycogen is the most immediate energy supply dur ...
... on carbohydrate as a source of fuel (40,45). In the face of enhanced glucose uptake by working muscles, blood glucose levels are maintained to a large extent by the breakdown of liver glycogen stores. Despite this enhanced liver glycogenolysis, muscle glycogen is the most immediate energy supply dur ...
Spinalin, a new glycine- and histidine
... wall is not known in detail, recent work has demonstrated the presence of unusual, small, collagen-like proteins in the wall (Kurz et al., 1991; Engel, 1997) and high-resolution electron microscopy has indicated that these are probably organized in collagen-like fibrils (Holstein et al., 1994). Noth ...
... wall is not known in detail, recent work has demonstrated the presence of unusual, small, collagen-like proteins in the wall (Kurz et al., 1991; Engel, 1997) and high-resolution electron microscopy has indicated that these are probably organized in collagen-like fibrils (Holstein et al., 1994). Noth ...
Localization of protein-binding sites within families of proteins
... We address the question of whether or not the positions of protein-binding sites on homologous protein structures are conserved irrespective of the identities of their binding partners. First, for each domain family in the Structural Classification of Proteins (SCOP), protein-binding sites are extra ...
... We address the question of whether or not the positions of protein-binding sites on homologous protein structures are conserved irrespective of the identities of their binding partners. First, for each domain family in the Structural Classification of Proteins (SCOP), protein-binding sites are extra ...
Fast and simple purification of GST fusion proteins using prepacked
... GSTrap affinity columns. A cytoplasmic extract from an E. coli culture expressing a GST fusion protein was loaded onto GSTrap 1 ml and GSTrap 5 ml columns (8 ml and 40 ml of lysate, respectively). Fusion protein was eluted using 10 mM reduced glutathione (lanes marked “GSTrap 1 ml” and “GSTrap 5 ml” ...
... GSTrap affinity columns. A cytoplasmic extract from an E. coli culture expressing a GST fusion protein was loaded onto GSTrap 1 ml and GSTrap 5 ml columns (8 ml and 40 ml of lysate, respectively). Fusion protein was eluted using 10 mM reduced glutathione (lanes marked “GSTrap 1 ml” and “GSTrap 5 ml” ...
Topology and Phosphorylation of Soybean Nodulin
... We are interested in the biogenesis of the peribacteroid membrane compartment in symbiotic nitrogen-fixing root nodules and the function of specific nodulins in the PBM. Because PBM nodulins are targeted to the PBM and not to the plasma membrane from which the PBM is derived, a specific mechanism ap ...
... We are interested in the biogenesis of the peribacteroid membrane compartment in symbiotic nitrogen-fixing root nodules and the function of specific nodulins in the PBM. Because PBM nodulins are targeted to the PBM and not to the plasma membrane from which the PBM is derived, a specific mechanism ap ...
The integrity of a cholesterol-binding pocket in Niemann–Pick C2
... known mechanism of its action prompted a genetic structure– function study of its properties. To this end, we purified wild-type and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from ...
... known mechanism of its action prompted a genetic structure– function study of its properties. To this end, we purified wild-type and mutant NPC2 proteins, starting from a culture medium into which cells had released NPC2. Previous studies of NPC2 used NPC2-conditioned media (7) or NPC2 purified from ...
A Mutation Causing Reduced Biological Activity and Stability of
... However, because the hormone is transported into cells in a free rather than TBG-bound form, TBG abnormalities have no effect on the metabolic state, general health, or survival of the individual (1, 2, 5-8). Nevertheless, inappropriate therapeutic interventions to correct the alterations of thyroid ...
... However, because the hormone is transported into cells in a free rather than TBG-bound form, TBG abnormalities have no effect on the metabolic state, general health, or survival of the individual (1, 2, 5-8). Nevertheless, inappropriate therapeutic interventions to correct the alterations of thyroid ...
Assessment of the mathematical issues involved
... paper, except where noted. Tracer is the labelled infused amino acid and tracee is the endogenous amino acid and the unlabelled infused amino acid. As we shall see, APE is the proper way to express enrichments in the specific case of measuring protein incorporation.) A correct derivation of an equat ...
... paper, except where noted. Tracer is the labelled infused amino acid and tracee is the endogenous amino acid and the unlabelled infused amino acid. As we shall see, APE is the proper way to express enrichments in the specific case of measuring protein incorporation.) A correct derivation of an equat ...