Comparative analysis of proteins with a mucus
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
Planta - University of Regina
... pathway with three C2-units from malonyl-CoA and regiospeci®c cyclization to give chalcone (Fig. 1) (Kreuzaler and Hahlbrock 1975). CHS is a typical homodimeric plant polyketide synthase with approx. 43kDa subunits. Based on the similarity in sequence and function, stilbene synthase (SchoÈppner and ...
... pathway with three C2-units from malonyl-CoA and regiospeci®c cyclization to give chalcone (Fig. 1) (Kreuzaler and Hahlbrock 1975). CHS is a typical homodimeric plant polyketide synthase with approx. 43kDa subunits. Based on the similarity in sequence and function, stilbene synthase (SchoÈppner and ...
Effect of surface hydrophobicity distribution on retention
... retention in HIC. The effect of the surface properties on protein retention has also been broached in a study about ion-exchange chromatography, where the effect of surface charge distribution of proteins was investigated [12]. Recently, the effect of surface hydrophobicity distribution of proteins ...
... retention in HIC. The effect of the surface properties on protein retention has also been broached in a study about ion-exchange chromatography, where the effect of surface charge distribution of proteins was investigated [12]. Recently, the effect of surface hydrophobicity distribution of proteins ...
embor2011116-sup-0001
... estimated repeatedly using a number of single or multiple mutations. Our review aimed at evaluating the correlation between such experimental data and those estimated in silico by several algorithms. The algorithms are, however, different in the type of predictions they provide. Two of the predictiv ...
... estimated repeatedly using a number of single or multiple mutations. Our review aimed at evaluating the correlation between such experimental data and those estimated in silico by several algorithms. The algorithms are, however, different in the type of predictions they provide. Two of the predictiv ...
Specific Isotopic Labeling of Methyl Groups has Extended the
... comprised of methyl groups. For proteins of known structure, this allows the binding sites for small molecules to be identified on the basis of binding-induced methyl group chemical shift perturbations. For small, 1H/13C ILV methyl-labeled proteins, 2D 1H-13C HSQC spectra can be recorded quickly (~1 ...
... comprised of methyl groups. For proteins of known structure, this allows the binding sites for small molecules to be identified on the basis of binding-induced methyl group chemical shift perturbations. For small, 1H/13C ILV methyl-labeled proteins, 2D 1H-13C HSQC spectra can be recorded quickly (~1 ...
Interplay of pH and Binding of Multivalent Metal Ions: Charge
... A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving force of ion-induced surface charge is the change in free energy upon ion condensation on the surface, which can be described ...
... A complete model for the protein charge in any system has to include both local and nonlocal contributions, although many studies focus on only one approach.36,37 The driving force of ion-induced surface charge is the change in free energy upon ion condensation on the surface, which can be described ...
Protein quality of linseed for growing broiler chicks
... considerable higher amount of sulfur-containing amino acids (methionine plus cystine). Results of the protein quality bioassay are presented in Table 3. The inclusion of linseed in the reference diet depressed body weight gain and feed utilization (feed intake:weight gain ratio). Regression analysis ...
... considerable higher amount of sulfur-containing amino acids (methionine plus cystine). Results of the protein quality bioassay are presented in Table 3. The inclusion of linseed in the reference diet depressed body weight gain and feed utilization (feed intake:weight gain ratio). Regression analysis ...
A novel multifunctional O-methyltransferase implicated in a dual
... column, and its purity was demonstrated by a single protein band of about 43 kDa, as estimated by SDSyPAGE. The molecular mass of the fusion protein was expected to be slightly higher than the calculated Mr of AEOMT due to the histidine-tagged amino acid residues. The N-terminal peptide of the purif ...
... column, and its purity was demonstrated by a single protein band of about 43 kDa, as estimated by SDSyPAGE. The molecular mass of the fusion protein was expected to be slightly higher than the calculated Mr of AEOMT due to the histidine-tagged amino acid residues. The N-terminal peptide of the purif ...
1st Sem (unit I)
... tyrosine have a polar hydroxyl group that take part in H- bonding, while cysteine and tyrosine can lose proton at alkaline pH. The side chain of asparagine and glutamine contain acid amide group. Side chains of two cysteines covalently crosslink to form a disulfide bond (-S-S-) c) Amino acids with a ...
... tyrosine have a polar hydroxyl group that take part in H- bonding, while cysteine and tyrosine can lose proton at alkaline pH. The side chain of asparagine and glutamine contain acid amide group. Side chains of two cysteines covalently crosslink to form a disulfide bond (-S-S-) c) Amino acids with a ...
The Predicted Candidates of Arabidopsis Plastid Inner Envelope
... number of amino acid residues within the transmembrane segments and also with the expected number of transmembrane segments in the protein. In other words, the more amino acid residues per predicted transmembrane segments and the more predicted number of transmembrane segments per gene protein, the ...
... number of amino acid residues within the transmembrane segments and also with the expected number of transmembrane segments in the protein. In other words, the more amino acid residues per predicted transmembrane segments and the more predicted number of transmembrane segments per gene protein, the ...
ARTÍCULOS
... H-NS–His recombinant protein and found that, as expected, it interacts with Hha. We used a Ni2ⴙ-nitrilotriacetic acid agarose method for affinity chromatography copurification of proteins to identify the H-NS protein of Y. enterocolitica. We constructed a six-His–YmoA recombinant protein derived from ...
... H-NS–His recombinant protein and found that, as expected, it interacts with Hha. We used a Ni2ⴙ-nitrilotriacetic acid agarose method for affinity chromatography copurification of proteins to identify the H-NS protein of Y. enterocolitica. We constructed a six-His–YmoA recombinant protein derived from ...
Early days of tRNA research: Discovery, function, purification and
... necessary to isolate longer fragments produced by limited digestion of the tRNA with T1-RNase or pancreatic RNase, carried out for short times, at reduced temperature and in the presence of Mg2+ to retain the tertiary structure of the tRNA (Litt and Ingram 1964; Nishimura and Novelli 1963). Under su ...
... necessary to isolate longer fragments produced by limited digestion of the tRNA with T1-RNase or pancreatic RNase, carried out for short times, at reduced temperature and in the presence of Mg2+ to retain the tertiary structure of the tRNA (Litt and Ingram 1964; Nishimura and Novelli 1963). Under su ...
TD3 5.08 – PKS/NRPS pathways and mass
... +358Da already dimethylated but not reduced. probably air oxidized from thiazoline to thiazole (probably does not occur in video) therefore-ketone reduction occurs AFTER dimethylation and condensation (HPTT to malony1) +378Da dimethylated but not yet cyclized therefore, dimethylation occuts BEFORE c ...
... +358Da already dimethylated but not reduced. probably air oxidized from thiazoline to thiazole (probably does not occur in video) therefore-ketone reduction occurs AFTER dimethylation and condensation (HPTT to malony1) +378Da dimethylated but not yet cyclized therefore, dimethylation occuts BEFORE c ...
Identification and functional analysis of a prokaryotic-type
... homogeneity from bacterial extracts by using His-tag affinity chromatography and were shown to be composed of single polypeptides of the expected molecular sizes, i.e. 53 kDa for PpAAT and 45 kDa for p-PpAAT (Figure 3b). The specific activity and catalytic constant of the homogenous p-PpAAT preparat ...
... homogeneity from bacterial extracts by using His-tag affinity chromatography and were shown to be composed of single polypeptides of the expected molecular sizes, i.e. 53 kDa for PpAAT and 45 kDa for p-PpAAT (Figure 3b). The specific activity and catalytic constant of the homogenous p-PpAAT preparat ...
Directions: Choose the BEST answer from among those given.
... e) choose this answer if none of these is the best choice 22)A common structural motif of proteins in which a linear sequence of amino acids folds into a righthanded elongated structure that twists in regular corkscrew fashion around a central axis, and is stabilized by internal hydrogen bonding bet ...
... e) choose this answer if none of these is the best choice 22)A common structural motif of proteins in which a linear sequence of amino acids folds into a righthanded elongated structure that twists in regular corkscrew fashion around a central axis, and is stabilized by internal hydrogen bonding bet ...
Immunoprecipitation protocol
... immunoprecipitation antibody, or normal serum (rabbit is often preferred, see Harlow and Lane, page 243) to 1 mL of lysate. Incubate for 1 h on ice. 2. Add 100 μL of bead slurry to the lysate. 3. Incubate for 10–30 min at 4°C with gentle agitation. 4. Spin in microcentrifuge at 14,000 x g at 4°C for ...
... immunoprecipitation antibody, or normal serum (rabbit is often preferred, see Harlow and Lane, page 243) to 1 mL of lysate. Incubate for 1 h on ice. 2. Add 100 μL of bead slurry to the lysate. 3. Incubate for 10–30 min at 4°C with gentle agitation. 4. Spin in microcentrifuge at 14,000 x g at 4°C for ...
Evolutionary Gain of Function for the ER Membrane
... (Rockford, IL). Antibodies against calnexin and protein disulfide isomerase (PDI) were from Stressgen (San Diego, CA); peroxidase-coupled anti-pentahistidine antibodies were from Qiagen (Hilden, Germany); peroxidase conjugate of anti-rabbit IgG goat antibodies were from Sigma (St. Louis, MO). Antibo ...
... (Rockford, IL). Antibodies against calnexin and protein disulfide isomerase (PDI) were from Stressgen (San Diego, CA); peroxidase-coupled anti-pentahistidine antibodies were from Qiagen (Hilden, Germany); peroxidase conjugate of anti-rabbit IgG goat antibodies were from Sigma (St. Louis, MO). Antibo ...
SpyTag and SpyCatcher - Department of Biochemistry
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
MEDICAL BIOCHEMISTRY Problem Unit One 1999/2000 pH and
... Hydrophobic compounds are uncharged, nonpolar species and generally contain largely aliphatic and aromatic organic groups. Hydrophilic compounds are polar and include sugars, salts, acids and bases, and polar organic groups such as amino, carboxyl, and alcohol groups. Many molecules become charged ( ...
... Hydrophobic compounds are uncharged, nonpolar species and generally contain largely aliphatic and aromatic organic groups. Hydrophilic compounds are polar and include sugars, salts, acids and bases, and polar organic groups such as amino, carboxyl, and alcohol groups. Many molecules become charged ( ...
Click Here to download this tutorial as a PDF
... The MDL Molfile (.mol) file format was originally designed as part of the Chemical MIME Project by Henry Rzepa. It is similar to .pdb files in that it contains the 3dimensional locations of atoms in a molecular structure. However, unlike .pdb files, .mol files are often used for smaller structures s ...
... The MDL Molfile (.mol) file format was originally designed as part of the Chemical MIME Project by Henry Rzepa. It is similar to .pdb files in that it contains the 3dimensional locations of atoms in a molecular structure. However, unlike .pdb files, .mol files are often used for smaller structures s ...
Proti-Ace Kit - Hampton Research
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
and Mountain Lions (Puma concolor) in North America
... identified by improved Akaike information criterion (AIC) values were considered significant only if subsequently supported by a Kishino-Hasegawa test result indicating significant topological incongruence between the trees constructed from the regions to the right and left of the breakpoint (alpha ...
... identified by improved Akaike information criterion (AIC) values were considered significant only if subsequently supported by a Kishino-Hasegawa test result indicating significant topological incongruence between the trees constructed from the regions to the right and left of the breakpoint (alpha ...
Homology Modeling a Fast Tool for Drug Discovery
... calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly conserved than its amino acid sequence, and that small or medium changes in sequence normally result in ...
... calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly conserved than its amino acid sequence, and that small or medium changes in sequence normally result in ...
Next generation sequencing
... the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively parallel sequencing generates 100 Mb to 1 Gb of short sequence reads in a single experime ...
... the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively parallel sequencing generates 100 Mb to 1 Gb of short sequence reads in a single experime ...
Structure and function of the chloroplast signal recognition particle
... Abstract The targeting of proteins, including the insertion and translocation of proteins in or across membranes, is a fundamental process within a cell, and a variety of specialized mechanisms for protein transport have been developed during evolution. The signal recognition particle (SRP) is found ...
... Abstract The targeting of proteins, including the insertion and translocation of proteins in or across membranes, is a fundamental process within a cell, and a variety of specialized mechanisms for protein transport have been developed during evolution. The signal recognition particle (SRP) is found ...