The OGCleaner: Detecting False
... Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
... Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
Sequence variation in the dihydrofolate reductase
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
Structural And Functional Studies Of Nicotinamide Adenine
... pathway leads to the formation of a quorum sensing molecule (SLS). We hypothesize that inhibition of the pathways will lead to GAS cell death or will impair the growth of the bacterium. This dissertation presents results of the functional and structural (using x-ray crystallography) characterization ...
... pathway leads to the formation of a quorum sensing molecule (SLS). We hypothesize that inhibition of the pathways will lead to GAS cell death or will impair the growth of the bacterium. This dissertation presents results of the functional and structural (using x-ray crystallography) characterization ...
Structural Basis of Biological Nitrogen Fixation
... 170 × 109 kg of ammonia8). By definition, biological nitrogen fixation must be achieved under physiological conditions of ∼290 K and 0.8 atm N2 which, consequently, suggests a higher degree of sophistication in chemical catalysis. The lower reaction temperature of the enzymatic process not only impl ...
... 170 × 109 kg of ammonia8). By definition, biological nitrogen fixation must be achieved under physiological conditions of ∼290 K and 0.8 atm N2 which, consequently, suggests a higher degree of sophistication in chemical catalysis. The lower reaction temperature of the enzymatic process not only impl ...
AMP-forming acetyl-CoA synthetases in Archaea show
... length, but insoluble, protein that was not characterized (data not shown). The M. thermautotrophicus ΔH genome sequence annotation for the gene encoding MT-ACS2 indicates that this gene is also interrupted by a stop codon, as for MT-ACS1, resulting in two adjacent ORFs (MTH1603-MTH1604) that togeth ...
... length, but insoluble, protein that was not characterized (data not shown). The M. thermautotrophicus ΔH genome sequence annotation for the gene encoding MT-ACS2 indicates that this gene is also interrupted by a stop codon, as for MT-ACS1, resulting in two adjacent ORFs (MTH1603-MTH1604) that togeth ...
Separation of Recombinant Human Erythropoietin (rEPO
... Recombinant human EPO protein is one of the most widely produced by many bio and pharmaceutical companies throughout the world for therapeutic agents. Erythropoietin protein (EPO) is a glycoprotein hormone found in plasma. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marr ...
... Recombinant human EPO protein is one of the most widely produced by many bio and pharmaceutical companies throughout the world for therapeutic agents. Erythropoietin protein (EPO) is a glycoprotein hormone found in plasma. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marr ...
BLAST Homepage and Selected Search Pages
... Eight score matrices from two families are available (A). The default BLOSUM62 matrix is the best general purpose matrix. For short queries, PAM30 is often selected. Each matrix has D its own set of supported gap penalties under E the Gap Costs pull-down menu (B). Scores for protein alignment can be ...
... Eight score matrices from two families are available (A). The default BLOSUM62 matrix is the best general purpose matrix. For short queries, PAM30 is often selected. Each matrix has D its own set of supported gap penalties under E the Gap Costs pull-down menu (B). Scores for protein alignment can be ...
Safety Assessment of Milk Proteins and Protein Derivatives as Used
... hydrolysis of casein…or lactalbumin (whey protein) (21 CFR §184.1553). Additionally, Casein is GRAS as substances migrating to food from paper and paperboard products (21CFR §182.90). Sodium Caseinate is GRAS for human and animal consumption (21CFR§182.1748, 21CFR§582.1748). Whey is GRAS for human c ...
... hydrolysis of casein…or lactalbumin (whey protein) (21 CFR §184.1553). Additionally, Casein is GRAS as substances migrating to food from paper and paperboard products (21CFR §182.90). Sodium Caseinate is GRAS for human and animal consumption (21CFR§182.1748, 21CFR§582.1748). Whey is GRAS for human c ...
A P element-homologous sequence in the house fly, Musca domestica
... was used as a probe in Southern blots of genomic DNA isolated from various strains of house flies. As seen in Fig. 6, the restriction pattern is static for all strains but one. This indicates that this sequence has been inactive for a considerable length of time, a conclusion consistent with the seq ...
... was used as a probe in Southern blots of genomic DNA isolated from various strains of house flies. As seen in Fig. 6, the restriction pattern is static for all strains but one. This indicates that this sequence has been inactive for a considerable length of time, a conclusion consistent with the seq ...
Vibrio diabolicus sp. nov., a New Polysaccharide
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
Adaptative biochemical pathways and regulatory networks in
... Generally, iron is one of the major limiting nutrients [11] and citrate-fermenting enterobacteria do not usually thrive on high concentrations of Fe(III)-citrate as sole carbon and energy source [12]. Indeed, there are habitats where the abundance of Fe(III) is so high, like in pyrite mine drainages ...
... Generally, iron is one of the major limiting nutrients [11] and citrate-fermenting enterobacteria do not usually thrive on high concentrations of Fe(III)-citrate as sole carbon and energy source [12]. Indeed, there are habitats where the abundance of Fe(III) is so high, like in pyrite mine drainages ...
Postexercise nutrient intake timing in humans is critical to recovery
... containing 1% t-butyldimethylchlorosilane (MtBSTFA ⫹ 1% tBDMCS; Regis Technologies, Morton Grove, IL). The derivatized samples were then analyzed with GC-MS (HewlettPackard 5890a GC and 5970 MS, San Fernando, CA) for plasma leucine and KIC enrichments. For determination of [2H5]glycerol enrichment, ...
... containing 1% t-butyldimethylchlorosilane (MtBSTFA ⫹ 1% tBDMCS; Regis Technologies, Morton Grove, IL). The derivatized samples were then analyzed with GC-MS (HewlettPackard 5890a GC and 5970 MS, San Fernando, CA) for plasma leucine and KIC enrichments. For determination of [2H5]glycerol enrichment, ...
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor
... Purified recombinant receptors were tested for autophosphorylation in vitro as described under “Experimental Procedures,” and results are shown in Fig. 2B. As previously reported (1), ETR1 required Mn2⫹ for autophosphorylation and did not function in the presence of Mg2⫹. ERS1 and ERS2 autophosphory ...
... Purified recombinant receptors were tested for autophosphorylation in vitro as described under “Experimental Procedures,” and results are shown in Fig. 2B. As previously reported (1), ETR1 required Mn2⫹ for autophosphorylation and did not function in the presence of Mg2⫹. ERS1 and ERS2 autophosphory ...
Classification and domain analysis of protein
... further subjected to manual curation to exclude any regulatory proteins that binds to a kinase. The final list of proteins fulfilling the above curation process was classified as true protein kinases and used in this study. All other proteins not having the above properties were filtered and elimina ...
... further subjected to manual curation to exclude any regulatory proteins that binds to a kinase. The final list of proteins fulfilling the above curation process was classified as true protein kinases and used in this study. All other proteins not having the above properties were filtered and elimina ...
Passenger Protein Determines Translocation Versus Retention in
... first 10 amino acids from the N terminus shows that translocation of aromatase into the ER did not require the Nterminal hydrophilic sequence. However, the observed weaker glycosylation suggests that its absence possibly interfered with the accessibility of OST to the glycosylation site. Thus, the o ...
... first 10 amino acids from the N terminus shows that translocation of aromatase into the ER did not require the Nterminal hydrophilic sequence. However, the observed weaker glycosylation suggests that its absence possibly interfered with the accessibility of OST to the glycosylation site. Thus, the o ...
Chicken eggshell matrix proteins related to anti
... associated genes has been identified and characterised. Experimental evidence suggests a role for matrix proteins in the process of shell mineralization. The eggshell matrix proteins can be divided into 3 groups according to the specificity of their expression i) some are egg white proteins, ii) oth ...
... associated genes has been identified and characterised. Experimental evidence suggests a role for matrix proteins in the process of shell mineralization. The eggshell matrix proteins can be divided into 3 groups according to the specificity of their expression i) some are egg white proteins, ii) oth ...
An acetate-sensitive mutant of Neurospora crassa deficient in acetyl
... cycle via citrate synthetase and ATP :citrate lyase, and acetylcarnitine interchangeable with acetyl-CoA through carnit ine-CoA acetyltransferase. These authors suggested that the latter enzyme, which they found to have a much higher affinity than acetyl-CoA hydrolase for acetyl-CoA, may be instrume ...
... cycle via citrate synthetase and ATP :citrate lyase, and acetylcarnitine interchangeable with acetyl-CoA through carnit ine-CoA acetyltransferase. These authors suggested that the latter enzyme, which they found to have a much higher affinity than acetyl-CoA hydrolase for acetyl-CoA, may be instrume ...
Three multidomain esterases from the cellulolytic
... (McDermid et al., 1990 ; Hespell & O’Bryan-Shah, 1988). There is little evidence so far for the involvement of esterase activities in cell wall degradation by Ruminococcus spp. (Akin et al., 1993), which represent one of the most numerous groups of cellulolytic bacteria in the rumen. Although it has ...
... (McDermid et al., 1990 ; Hespell & O’Bryan-Shah, 1988). There is little evidence so far for the involvement of esterase activities in cell wall degradation by Ruminococcus spp. (Akin et al., 1993), which represent one of the most numerous groups of cellulolytic bacteria in the rumen. Although it has ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... annotation of new DNA and protein sequences are often based on the assumption that only the sequence is known, and try to predict various features such as DNA exons and introns, protein domains or subcellular location using only sequence as input. Thus, there is a certain rationale for developing pr ...
... annotation of new DNA and protein sequences are often based on the assumption that only the sequence is known, and try to predict various features such as DNA exons and introns, protein domains or subcellular location using only sequence as input. Thus, there is a certain rationale for developing pr ...
Proteomics Principles and Techniques Prof. Sanjeeva Srivastava
... Proteins can performs various type of functions whether it is catalysis, movement, structure, regulation, signaling, transport etcetera. As you can see in this slide, various types of functions have been shown. Enzyme catalysis - the enzyme catalyze biochemical reactions by increasing the rate of re ...
... Proteins can performs various type of functions whether it is catalysis, movement, structure, regulation, signaling, transport etcetera. As you can see in this slide, various types of functions have been shown. Enzyme catalysis - the enzyme catalyze biochemical reactions by increasing the rate of re ...
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF
... of lipids between membranes in vitro. The biological role of ns-LTPs is still a matter of debate. They are subdivided into two families: ns-LTP1 and ns-LTP2. 4,23,24 Non-specific LTP1 is an abundant protein of the aleurone layers from barley endosperm and has been studied more thoroughly than ns-LTP ...
... of lipids between membranes in vitro. The biological role of ns-LTPs is still a matter of debate. They are subdivided into two families: ns-LTP1 and ns-LTP2. 4,23,24 Non-specific LTP1 is an abundant protein of the aleurone layers from barley endosperm and has been studied more thoroughly than ns-LTP ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... have demonstrated that microtubules and actin micro®laments both contribute to the morphology of stromules (Kwok and Hanson, 2003). Stromule motility, similar to chloroplast movement, is driven by actin micro®laments, with an inhibitory contribution from microtubules (Gray et al., 2001; Kwok and Han ...
... have demonstrated that microtubules and actin micro®laments both contribute to the morphology of stromules (Kwok and Hanson, 2003). Stromule motility, similar to chloroplast movement, is driven by actin micro®laments, with an inhibitory contribution from microtubules (Gray et al., 2001; Kwok and Han ...
Descriptions of translation related genes that
... Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism and transcription Part of 66S ribosomal particle, participate in ribosome assembly and maintenance Nuclear pore subunit, interact with Mex67p and involved in RNA pro ...
... Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism and transcription Part of 66S ribosomal particle, participate in ribosome assembly and maintenance Nuclear pore subunit, interact with Mex67p and involved in RNA pro ...
Amino acids used in Animal Nutrition
... • Those that cannot be synthesized by the animal in quantities sufficient for optimum performance and must be supplied in the feed Non-essential (dispensable): • Those that do not have to be provided in the diet and that can be synthesized by the body. All amino acids are important and must be p ...
... • Those that cannot be synthesized by the animal in quantities sufficient for optimum performance and must be supplied in the feed Non-essential (dispensable): • Those that do not have to be provided in the diet and that can be synthesized by the body. All amino acids are important and must be p ...
C-terminal Truncation of p21H Preserves Crucial Kinetic and
... T4 DNA Ligase,polynucleotide kinase, and dNTPswere from Boehringer Mannheim, Federal Republic of Germany. The above reagents were used as described in the laboratory manual of Maniatis et al. (18).Transformation was done according to themethod of Hanahan (19) with frozen cells or according to theCaC ...
... T4 DNA Ligase,polynucleotide kinase, and dNTPswere from Boehringer Mannheim, Federal Republic of Germany. The above reagents were used as described in the laboratory manual of Maniatis et al. (18).Transformation was done according to themethod of Hanahan (19) with frozen cells or according to theCaC ...