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NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED

... the glycolytic pathway. Genomic and proteomic analyses identified three aldolase A (Aldoa) transcripts generated by retrotransposition and alternative splicing: Aldoa_v2, Aldoart1, and Aldoart2. Unique nucleotide sequence in Aldoart1 provided evidence for an alternatively spliced exon in Aldoa_v2. E ...
Snorks Lab File
Snorks Lab File

... In this simulation, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with eight genes on it. Your job is to analyze the genes of its DNA and determine what traits the organ ...
Strand
Strand

... Understand how the manipulation methods above result in offspring 13-2 Pages 322 genetic engineering Understand the use of technology in the manipulation of genes: genetic engineering, genome, 13-1 Page 320 Inbreeding recombinant DNA, DNA sequencing, electrophoresis, DNA fingerprinting 13-1 Page 320 ...
Differential scanning calorimetry as tool in observing thermal and
Differential scanning calorimetry as tool in observing thermal and

... Introduction The mechanisms of protein degradation by chemical or physical means have been extensively reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure ...
Exploring the Biosynthetic Potential of Cystobacter fuscus
Exploring the Biosynthetic Potential of Cystobacter fuscus

... the portion of natural product derived small molecules (34%) tells a different story. Also taking into account the compounds that are synthetically derived but simulate a natural product or its pharmacophore (30%) this adds up to 64% of the total number of 1073 small molecule approved drugs (Figure ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
Taxonomic characterization of Ochrobactrum sp. isolates from soil

... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides

... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
genetic disorders associated with macrocephaly
genetic disorders associated with macrocephaly

... include a prominent or high forehead and a dolichocephalic head shape. Increased width of the cranial base can at times produce mild hypertelorism and down slanting palpebral fissures. Also, the facial area may be relatively small giving a triangular craniofacial appearance. Finally, nongenetic macr ...
Beta-ketothiolase deficiency
Beta-ketothiolase deficiency

... severe hypoglycemia, had elevated lactate level, mild hyperammonemia, and ketonuria on the second day of life. No further acidotic episode occurred. The patient was severely retarded with marked restless, choreathetoid movements, marked hypotonia. In these 2 years, several patients with this disorde ...
promoting training adaptations through nutritional
promoting training adaptations through nutritional

... Pilegaard et al. 2000). Indeed, it appears that for many exercise-related genes, the time-course of transcriptional activation occurs during the first few hours of recovery (Pilegaard et al. 2000) and may be linked by common signalling and/or regulatory mechanisms to the restoration of muscle energy ...
representation and display of non-standard peptides using semi
representation and display of non-standard peptides using semi

... • The solution to this problem is the systematic naming of amino acid derivatives. • This approach is well known to peptide chemists, with these names routinely used in scientific articles, patent applications and supplier catalogues, and easily recognizable by chemists. • Alas this “de facto” nomen ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
Product description P003-D1 MLH1-MSH2-v01 - MRC

... µl SD052 DNA in MLPA experiments is recommended as it can be used to aid in data binning of the peak pattern using Coffalyser.Net software. Binning DNA should never be used as a reference sample in the MLPA data analysis. Neither should it be used in quantification of mutation signals, as for this p ...
Genetic Control of Root Hair Development in
Genetic Control of Root Hair Development in

... product is required early in hair development. Each of the three other genes appears to be involved in some aspect of root hair elongation. Of these, the RHD2 gene product appears to be required before either RHD3 or RHD4 because the rhd3 and rhd4 mutants produce hairs that are able to elongate (alb ...
Quantitative Analysis of the Kinetics of End
Quantitative Analysis of the Kinetics of End

... addition, the binding of ATP or ATPgS to individual ®lament subunits can stimulate NTP hydrolysis in neighboring subunits (Lee & Cox, 1990; Menge & Bryant, 1988). These interactions tend to maintain the entire ®lament in an extended active state. However, the ATP hydrolytic cycles of adjacent monome ...
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill

... population fails to achieve the daily minimum recommendation of 30 min moderate intensity of physical activity, and within these people the risk of getting the related chronic diseases including type 2 diabetes increases by 1.5 times [4]. Therefore, it has become valuable to discover active agents t ...
Completion of a parasexual cycle in Candida
Completion of a parasexual cycle in Candida

... locus in 32 colonies picked at random from YEPD revealed that 31 of the 32 colonies were still tetra-allelic at MTL and one was tri-allelic, consistent with relatively stable propagation of the tetraploid on this medium. In comparison, analysis of 32 colonies randomly picked from pre-spo plates indi ...
Multiplex in-vitro Detection using SERS
Multiplex in-vitro Detection using SERS

Cyclooxygenase mechanisms Lawrence J Marnett
Cyclooxygenase mechanisms Lawrence J Marnett

... different fatty acid hydroperoxides react with COX to generate Compound I, so lipid hydroperoxides are likely activators [40,41•]. Peroxynitrite, the coupling product of NO and superoxide anion, is also an efficient substrate for the peroxidase of both COX-1 and COX-2 [42]. It activates the cyclooxy ...
Regulation of Cytochrome bd Expression in Mycobacterium
Regulation of Cytochrome bd Expression in Mycobacterium

... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
Genetic variation at RYR1, IGF2, FUT1, MUC13, and KPL2
Genetic variation at RYR1, IGF2, FUT1, MUC13, and KPL2

... c.1843C>T mutation in 1991 (Fujii et al., 1991) many breeding companies have used the DNA diagnostic test to eliminate homozygous stress positive (TT) individuals and heterozygous carriers (TC). Therefore, the high frequency of the C allele can be explained by selection. It is noteworthy that a numb ...
Glutathione Breakthrough: Advancement in
Glutathione Breakthrough: Advancement in

... Glutathione (GSH) is one of the most critical molecules of the entire body. GSH plays an important role in antioxidant defense, nutrient metabolism and is key in a vast number of cellular processes including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transdu ...
Genetic Analysis of Muscle Development in Drosophila
Genetic Analysis of Muscle Development in Drosophila

... We set out to explore the genetic basis of these specificities by means of a genetic analysis of these loci. Within the context of the genetic analysis, specificity should be understood as a property of the genome to control a biological process (muscle fasciculation in this case) distinct from othe ...
thèse - Université Evry Val d`Essonne
thèse - Université Evry Val d`Essonne

... proteins. mtDNA generally encodes catalytic subunits of the complexes of the respiratory chain with tRNA and rRNA components of the mitochondrial translation system, but it can also contain additional proteins; in S. cerevisiae, besides seven subunits of complexes involved in oxidative phosphorylati ...
Single Nucleotide Polymorphism (SNP) of the Endothelial Nitric
Single Nucleotide Polymorphism (SNP) of the Endothelial Nitric

... Reports have shown eNOS as a cytoplasmic protein in Leydig and Sertoli cells as well as in all stages of spermatogenesis and in epithelium of the epididymis and vas deferens (Burnett et al, 1995; Rosselli et al, 1998). A single nucleotide polymorphism (SNP) of the eNOS gene within exon 7 includes a ...
Transductional Analysis of Arginineless Mutants in Proteus rnirabilis
Transductional Analysis of Arginineless Mutants in Proteus rnirabilis

... Linkage of other markers to arginine genes. Linked transduction of an argF and a pyrimidine marker has been described (Prozesky & Coetzee, 1966). In crosses between arginineless mutants and representative cysteineless and methionineless mutants of Grabow & Smit (1967) donor-type transduction occurre ...
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Point mutation



A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.
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