Modelling and parameter estimation of bacterial growth with
... Measure the lag (LN) by means of the fitted bi-phasic linear function generated by N initial cells: y(t) = y0 + max( µ(t-LN), 0) y0=lnN We call the obtained L-value as the “geometrical” lag, due to its definition. If N=1, we arrive at the single cell lag time, L1. It is not the same as the physiolog ...
... Measure the lag (LN) by means of the fitted bi-phasic linear function generated by N initial cells: y(t) = y0 + max( µ(t-LN), 0) y0=lnN We call the obtained L-value as the “geometrical” lag, due to its definition. If N=1, we arrive at the single cell lag time, L1. It is not the same as the physiolog ...
TEC-FAMILY KINASES: REGULATORS OF T-HELPER
... • The TEC-family kinases, they have an amino-terminal PtdIns(3,4,5)P3 binding PH domain, which is followed by a TEC-homology domain that contains one or two proline-rich regions (PRRs), then SRC homology 3 (SH3) and SH2 proteininteraction domains, and a carboxyterminal kinase domain. • The atypical ...
... • The TEC-family kinases, they have an amino-terminal PtdIns(3,4,5)P3 binding PH domain, which is followed by a TEC-homology domain that contains one or two proline-rich regions (PRRs), then SRC homology 3 (SH3) and SH2 proteininteraction domains, and a carboxyterminal kinase domain. • The atypical ...
Effect of Steroid Hormones and Retinoids on the Formation of
... Effect of Steroid Hormones and Retinoids on the Formation of Capillary-Like Tubular Structures of Human Microvascular Endothelial Cells in Fibrin Matrices Is Related to Urokinase Expression By Mirian Lansink, Pieter Koolwijk, Victor van Hinsbergh, and Teake Kooistra Angiogenesis, the formation of ne ...
... Effect of Steroid Hormones and Retinoids on the Formation of Capillary-Like Tubular Structures of Human Microvascular Endothelial Cells in Fibrin Matrices Is Related to Urokinase Expression By Mirian Lansink, Pieter Koolwijk, Victor van Hinsbergh, and Teake Kooistra Angiogenesis, the formation of ne ...
Targeting the Folate Receptor, a Novel Cancer
... of the most effective conjugation for a targeting strategy. Folate, a salt derivative of Folic Acid, is rapidly gaining acceptance as a targeting ligand for cancer treatments utilizing nanotechnology. In order to optimize a nanoparticle based treatment for cancer, an intense literature review was co ...
... of the most effective conjugation for a targeting strategy. Folate, a salt derivative of Folic Acid, is rapidly gaining acceptance as a targeting ligand for cancer treatments utilizing nanotechnology. In order to optimize a nanoparticle based treatment for cancer, an intense literature review was co ...
Cytotoxicity of resin monomers on human gingival - GCP
... The Alamar Blue assay incorporates a fluorometric/colorimetric growth indicator based on detection of metabolic activity. The system incorporates an oxidation–reduction indicator that both fluoresces and changes colour in response to chemical reduction of growth medium resulting from cell growth [8]. ...
... The Alamar Blue assay incorporates a fluorometric/colorimetric growth indicator based on detection of metabolic activity. The system incorporates an oxidation–reduction indicator that both fluoresces and changes colour in response to chemical reduction of growth medium resulting from cell growth [8]. ...
fibulins: a versatile family of extracellular matrix proteins
... The identification of a new family of ECM proteins usually generates questions about their specific functions and their associations with different tissue compartments as an initial approach to understanding their biological role. Information regarding the tissue deposition of all of the fibulin iso ...
... The identification of a new family of ECM proteins usually generates questions about their specific functions and their associations with different tissue compartments as an initial approach to understanding their biological role. Information regarding the tissue deposition of all of the fibulin iso ...
full lab details and projects
... ultrastructure arl13b mutants and genetic mosaic analysis to examine the long-term fate of mutant photoreceptors in wild type zebrafish. To determine relationship between Arl13b and PCP signaling, both genetic and physical interactions will be tested. Finally, the requirement for GTPase activity and ...
... ultrastructure arl13b mutants and genetic mosaic analysis to examine the long-term fate of mutant photoreceptors in wild type zebrafish. To determine relationship between Arl13b and PCP signaling, both genetic and physical interactions will be tested. Finally, the requirement for GTPase activity and ...
PDF
... K~2. And this requires less order or energy than a composite cell, of dimension K~3. Thus, maintaining systems of high order and complexity intrinsically require cell states of both high dimensionality and, by Eq. (2b), energy resource rate. Requirement of non-uniform cell structure. Unlike crystals ...
... K~2. And this requires less order or energy than a composite cell, of dimension K~3. Thus, maintaining systems of high order and complexity intrinsically require cell states of both high dimensionality and, by Eq. (2b), energy resource rate. Requirement of non-uniform cell structure. Unlike crystals ...
Cells in Vitro - Cancer Research
... The cause of resistance in S. faecalis has been shown to be due to one major (5) and one minor (6) canavanine-destroying enzyme. The former catalyzed the reductive cleavage of canavanine to guanidine and homoserine. Apparently, this en zyme is not present in the Walker 256 cells. Fur thermore, the p ...
... The cause of resistance in S. faecalis has been shown to be due to one major (5) and one minor (6) canavanine-destroying enzyme. The former catalyzed the reductive cleavage of canavanine to guanidine and homoserine. Apparently, this en zyme is not present in the Walker 256 cells. Fur thermore, the p ...
Regulatory role of rpL3 in cell response to nucleolar stress induced
... Fig. 2C-D in untreated cells the rpL3 silencing did not cause any significant alteration on p21 promoter activity compared to untreated cells transfected with scrambled siRNA. The treatment with Act D increased p21 gene promoter activity. When rpL3 expression was switched off, the effect on p21 gene ...
... Fig. 2C-D in untreated cells the rpL3 silencing did not cause any significant alteration on p21 promoter activity compared to untreated cells transfected with scrambled siRNA. The treatment with Act D increased p21 gene promoter activity. When rpL3 expression was switched off, the effect on p21 gene ...
Ectodermal progenitors derived from epiblast
... expression of the neural marker MAP2 or the epidermal marker K18 was clearly detected in A-83-01-treated cells without or with BMP4, respectively (Supplementary Figure S2A). Interestingly, it is the cells treated with A-83-01 for 15 h or 18 h that display the dual potency for neural or epidermal fat ...
... expression of the neural marker MAP2 or the epidermal marker K18 was clearly detected in A-83-01-treated cells without or with BMP4, respectively (Supplementary Figure S2A). Interestingly, it is the cells treated with A-83-01 for 15 h or 18 h that display the dual potency for neural or epidermal fat ...
Research Project Final Report
... The primary aim of this project was to identify cell lines directly permissive to ovine scrapie by further investigation of existing cell lines with proven ability to propagate sheep scrapie, through the development and characterisation of further Rov lines expressing natural ovine PrP alleles and n ...
... The primary aim of this project was to identify cell lines directly permissive to ovine scrapie by further investigation of existing cell lines with proven ability to propagate sheep scrapie, through the development and characterisation of further Rov lines expressing natural ovine PrP alleles and n ...
``Biology of Cultured Cells``. In: Culture of Animal Cells, 5th Edition
... Subsequently, it was shown that cells would attach to some plastics, such as polystyrene, if the plastic was appropriately treated with an electric ion discharge or high-energy ionizing radiation. We now know that cell adhesion is mediated by specific cell surface receptors for molecules in the extr ...
... Subsequently, it was shown that cells would attach to some plastics, such as polystyrene, if the plastic was appropriately treated with an electric ion discharge or high-energy ionizing radiation. We now know that cell adhesion is mediated by specific cell surface receptors for molecules in the extr ...
In vitro control of neuronal polarity by
... 1.5 fig ml" 1 polyornithine were cultured for 3 days, fixed for 1 hour at 4°C with paraformaldehyde (4% in PBS), rinsed in PBS-glycine 5 mM pH 7.4, incubated (1 hour 37°C) with antiMAP2 antibodies (1/400) in PBS plus 10% FCS and 0.01% saponin (buffer A), washed 3 times and further incubated for 1 ho ...
... 1.5 fig ml" 1 polyornithine were cultured for 3 days, fixed for 1 hour at 4°C with paraformaldehyde (4% in PBS), rinsed in PBS-glycine 5 mM pH 7.4, incubated (1 hour 37°C) with antiMAP2 antibodies (1/400) in PBS plus 10% FCS and 0.01% saponin (buffer A), washed 3 times and further incubated for 1 ho ...
Glucosamine induces cell-cycle arrest and hypertrophy of mesangial
... cause of end-stage renal disease. DN was found to affect approx. 30 % of patients who have suffered from diabetes for 20 years and exposes them to a higher rate of mortality. The pathology is characterized by complex structural changes including renal hypertrophy, basement membrane thickening and pr ...
... cause of end-stage renal disease. DN was found to affect approx. 30 % of patients who have suffered from diabetes for 20 years and exposes them to a higher rate of mortality. The pathology is characterized by complex structural changes including renal hypertrophy, basement membrane thickening and pr ...
Slides - Institute for Evidence
... 6) Assay competence in disease mouse models. 7) Test in humans as for lysosomal storage diseases. ...
... 6) Assay competence in disease mouse models. 7) Test in humans as for lysosomal storage diseases. ...
3 - Rudner Lab - Harvard University
... the assembly of the bacterial flagellum in which the activation of a transcription factor is coupled to the completion of an intermediate structure in flagellum assembly. We then turn in more depth to two emerging examples of morphological coupling that operate during the process of sporulation. In ...
... the assembly of the bacterial flagellum in which the activation of a transcription factor is coupled to the completion of an intermediate structure in flagellum assembly. We then turn in more depth to two emerging examples of morphological coupling that operate during the process of sporulation. In ...
Patterns of Nogo mRNA and Protein Expression in the Developing
... expression analysis is therefore limited. For immunohistochemistry the antibodies were diluted in PBS, pH 7.4, containing 1% normal goat serum as follows: mAb I N-1 hybridoma supernatant, 1:5; AS Bruna, 1:7500; AS Bianca, 1:2000; AS 472, 1:2000; AS 818, 1:2000; affinitypurified AS 818, 1:50; anti-my ...
... expression analysis is therefore limited. For immunohistochemistry the antibodies were diluted in PBS, pH 7.4, containing 1% normal goat serum as follows: mAb I N-1 hybridoma supernatant, 1:5; AS Bruna, 1:7500; AS Bianca, 1:2000; AS 472, 1:2000; AS 818, 1:2000; affinitypurified AS 818, 1:50; anti-my ...
Induction of fungal cell wall stress
... family of five 1,3--D-glucan synthase-encoding genes in A. niger. To further study the antifungal activity of Humulus lupulus and Tectona grandis extracts shown in Chapter 3 the mode of action was tested. A transgenic Aspergillus niger is used as a model for the induction of fungal cell wall stress ...
... family of five 1,3--D-glucan synthase-encoding genes in A. niger. To further study the antifungal activity of Humulus lupulus and Tectona grandis extracts shown in Chapter 3 the mode of action was tested. A transgenic Aspergillus niger is used as a model for the induction of fungal cell wall stress ...
