Amino Acids, Proteins, and Enzymes
... For more information on the source of this book, or why it is available for free, please see the project's home page (http://2012books.lardbucket.org/). You can browse or download additional books there. ...
... For more information on the source of this book, or why it is available for free, please see the project's home page (http://2012books.lardbucket.org/). You can browse or download additional books there. ...
Spring mechanics of α-helical polypeptide
... considerably more serious error in estimation of L from S than does a horizontal one. If we assume that a randomly coiled (Glu)n-Cys can be approximated by a Gaussian chain with fixed bond angles but with free internal rotations, the expected average of its end-to-end distance is ~3.7 nm, which is e ...
... considerably more serious error in estimation of L from S than does a horizontal one. If we assume that a randomly coiled (Glu)n-Cys can be approximated by a Gaussian chain with fixed bond angles but with free internal rotations, the expected average of its end-to-end distance is ~3.7 nm, which is e ...
Post-Translational Processing (7.1)
... Most glycosylated proteins are either secreted or remain membrane-bound Glycosylation is the most abundant form of post-translational modification Glycosylation confers resistance to protease digestion by steric protection Important in cell-cell recognition ...
... Most glycosylated proteins are either secreted or remain membrane-bound Glycosylation is the most abundant form of post-translational modification Glycosylation confers resistance to protease digestion by steric protection Important in cell-cell recognition ...
... extract was very active in hydrolysing PP, in the presence of Mg2+;less activity was obtained with tripolyphosphate or trimetaphosphate as substrate, and these activities may have been due in part to PP, contamination of the substrates. The enzyme did not hydrolyse organic pyrophosphates, indicating ...
Hydrogel-Encapsulated Fluorophore-Enzyme Conjugates for Direct
... fluorescence emission ratio at 550 and 620 nm resulting from excitation at 510 nm. The acid peak fluorescent intensity increased with paraoxon concentration while spectra continued to pass through an isoemissive point at 620 nm, resulting in increasing fluorescent intensity ratios with higher concen ...
... fluorescence emission ratio at 550 and 620 nm resulting from excitation at 510 nm. The acid peak fluorescent intensity increased with paraoxon concentration while spectra continued to pass through an isoemissive point at 620 nm, resulting in increasing fluorescent intensity ratios with higher concen ...
Essentials of Glycobiology Lecture 13 April 25th. 2000
... • Soluble "corrective factors" turned out be different lysosomal enzymes deficient in patients with different diseases - being secreted by the normal cells in small amounts • Enzymes found to exist in two forms: a "high-uptake" form that could correct deficient cells, and a "low-uptake" form that wa ...
... • Soluble "corrective factors" turned out be different lysosomal enzymes deficient in patients with different diseases - being secreted by the normal cells in small amounts • Enzymes found to exist in two forms: a "high-uptake" form that could correct deficient cells, and a "low-uptake" form that wa ...
Mechanism of Thymidylate Synthase, Cont`d
... Dehydrogenase • GAPDH is one of the key enzymes for glycolysis, reversibly catalyzes the first glycolytic reaction to involve oxidation-reduction • It converts the glyceraldehyde-3-phosphate (G3P) into the high energy phosphate compound, 1,3 bisphosphoglycerate (BPG), using NAD+ as a cofactor • BPG ...
... Dehydrogenase • GAPDH is one of the key enzymes for glycolysis, reversibly catalyzes the first glycolytic reaction to involve oxidation-reduction • It converts the glyceraldehyde-3-phosphate (G3P) into the high energy phosphate compound, 1,3 bisphosphoglycerate (BPG), using NAD+ as a cofactor • BPG ...
Chapter 25 Chapter Topics Fatty Acid Biosynthesis
... • Different “Families” according to distance of last double bond from methyl end: • ω-9 (oleic acid, 9-C18:1) • ω-7 (palmitic acid, 9-C16:1) • ω-6 (linoleic acid, 9,12-C18:2)—only from plants. • Arachidonic acid made from dietary linoleic acid ...
... • Different “Families” according to distance of last double bond from methyl end: • ω-9 (oleic acid, 9-C18:1) • ω-7 (palmitic acid, 9-C16:1) • ω-6 (linoleic acid, 9,12-C18:2)—only from plants. • Arachidonic acid made from dietary linoleic acid ...
Crystal Structures of the Oxidized and Reduced Forms of UDP
... fashion. While it has been postulated that the active site of epimerase must contain a base, the only potential candidates within approximately 5 Å of both the NAD+ and the UDP are Asp 31, Asp 58, and Asp 295. These amino acid residues, however, are intimately involved in nucleotide binding and most ...
... fashion. While it has been postulated that the active site of epimerase must contain a base, the only potential candidates within approximately 5 Å of both the NAD+ and the UDP are Asp 31, Asp 58, and Asp 295. These amino acid residues, however, are intimately involved in nucleotide binding and most ...
Antimicrobial Agents and Chemotherapy
... scarcely been examined; however, inactivation of kanamycin by a resistant strain of Staphylococcus aureus has been reported (5). This phenomenon was later demonstrated to be plasmid mediated (16). A lividomycin phosphotransferase has also been isolated from S. aureus strains (7). The phosphorylated ...
... scarcely been examined; however, inactivation of kanamycin by a resistant strain of Staphylococcus aureus has been reported (5). This phenomenon was later demonstrated to be plasmid mediated (16). A lividomycin phosphotransferase has also been isolated from S. aureus strains (7). The phosphorylated ...
Gene Section GHRL (ghrelin/obestatin prepropeptide) Atlas of Genetics and Cytogenetics
... As the endogenous ligand of the growth hormone secretagogues (GHS) receptor, Ghrelin/MTLRP is involved in the pulsatile secretion of Growth hormone. In addition to this role Ghrelin/MTLRP is in the regulation of feeding. In rodent In contrast to leptin, Ghrelin/MTLRP promotes food intake and obesity ...
... As the endogenous ligand of the growth hormone secretagogues (GHS) receptor, Ghrelin/MTLRP is involved in the pulsatile secretion of Growth hormone. In addition to this role Ghrelin/MTLRP is in the regulation of feeding. In rodent In contrast to leptin, Ghrelin/MTLRP promotes food intake and obesity ...
Phytanic acid omega-oxidation in human liver microsomes
... J.C. KOMEN, M. DURAN and R.J.A. WANDERS ...
... J.C. KOMEN, M. DURAN and R.J.A. WANDERS ...
THE EXTRACTION OF PAPAIN FROM PAPAYA LEAVES NUR
... solution. Patient of Giant Capillary Conjunctivitis (GPC) is advised to at least clean their contact lens with proteolytic enzyme papain solution once a week to remove deposits and quieting GPC (National Research Council, 1991). Traditionally, some of the plant extracts including papaya have been us ...
... solution. Patient of Giant Capillary Conjunctivitis (GPC) is advised to at least clean their contact lens with proteolytic enzyme papain solution once a week to remove deposits and quieting GPC (National Research Council, 1991). Traditionally, some of the plant extracts including papaya have been us ...
Plant aromatic amino acid decarboxylases
... plant species results in a multiplicity of functionally diverse AAAD enzymes. A single AAAD annotation does not accurately represent the selection of activities and substrate specificities. This can be illustrated through our recent study of some predicted Drosophila and mosquito AAAD sequences. Res ...
... plant species results in a multiplicity of functionally diverse AAAD enzymes. A single AAAD annotation does not accurately represent the selection of activities and substrate specificities. This can be illustrated through our recent study of some predicted Drosophila and mosquito AAAD sequences. Res ...
Lecture 6 - TCA cycle I - University of Lethbridge
... Why such a complex set of enzymes? 1. Enzymatic reaction rates are limited by diffusion, with shorter distance between subunits in an enzyme, the substrate can be directed from one subunit (catalytic site) to another. ...
... Why such a complex set of enzymes? 1. Enzymatic reaction rates are limited by diffusion, with shorter distance between subunits in an enzyme, the substrate can be directed from one subunit (catalytic site) to another. ...
16. enzymes i – nomenclature and classification
... With the continuous increase in our knowledge of enzymology, various systems have evolved to name and classify the enzymes, using one or the other criterion as the basis. However, many of the enzymes were known before these systems of naming enzymes were adopted. The names of such enzymes were not c ...
... With the continuous increase in our knowledge of enzymology, various systems have evolved to name and classify the enzymes, using one or the other criterion as the basis. However, many of the enzymes were known before these systems of naming enzymes were adopted. The names of such enzymes were not c ...
Catalytic triad
A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.