A Pneumocystis carinii multi-gene family with
... Copies of a multi-gene family, named PRTl (protease 3,encoding a subtilisinlike serine protease were cloned from the opportunistic fungal pathogen Pneumocystis carinii. Comparison of the nucleotide sequence of a genomic clone and a cDNA clone of PRTl from P. carinii f. sp. carinii revealed the prese ...
... Copies of a multi-gene family, named PRTl (protease 3,encoding a subtilisinlike serine protease were cloned from the opportunistic fungal pathogen Pneumocystis carinii. Comparison of the nucleotide sequence of a genomic clone and a cDNA clone of PRTl from P. carinii f. sp. carinii revealed the prese ...
Plant Physiology 66:
... previously (14) showed that external NH4' would not support growth as sole N source. These observations suggest that the peripheral cells of the embryo admit NH4' only at a very slow rate. This conclusion is supported by the present observations on the changes in GDH and GS activities of developing ...
... previously (14) showed that external NH4' would not support growth as sole N source. These observations suggest that the peripheral cells of the embryo admit NH4' only at a very slow rate. This conclusion is supported by the present observations on the changes in GDH and GS activities of developing ...
Binding of the EcoRII methyltransferase to 5
... addition to forming part of, or being adjacent to, the AdoMet binding site, also forms part of the DNA binding site. ...
... addition to forming part of, or being adjacent to, the AdoMet binding site, also forms part of the DNA binding site. ...
Glycolysis
... The tense conformation of PFK, at high [ATP], has lower affinity for the other substrate, fructose-6-P. Sigmoidal dependence of reaction rate on [fructose-6-P] is seen. AMP, present at significant levels only when there is extensive ATP hydrolysis, antagonizes effects of high ATP. ...
... The tense conformation of PFK, at high [ATP], has lower affinity for the other substrate, fructose-6-P. Sigmoidal dependence of reaction rate on [fructose-6-P] is seen. AMP, present at significant levels only when there is extensive ATP hydrolysis, antagonizes effects of high ATP. ...
Glycolysis
... The tense conformation of PFK, at high [ATP], has lower affinity for the other substrate, fructose-6-P. Sigmoidal dependence of reaction rate on [fructose-6-P] is seen. AMP, present at significant levels only when there is extensive ATP hydrolysis, antagonizes effects of high ATP. ...
... The tense conformation of PFK, at high [ATP], has lower affinity for the other substrate, fructose-6-P. Sigmoidal dependence of reaction rate on [fructose-6-P] is seen. AMP, present at significant levels only when there is extensive ATP hydrolysis, antagonizes effects of high ATP. ...
Identification of catalytically essential amino acid residues and immobilization Rumex
... Broadening of the pH curve, shifts of pH-optimum and enhanced tolerance to unfavorable conditions are well known properties of many immobilized enzymes [7 ,2 7 ]. The fixed position of immobilized enzyme on the surface of the carrier is thought to prevent extensive distortion of the native conformat ...
... Broadening of the pH curve, shifts of pH-optimum and enhanced tolerance to unfavorable conditions are well known properties of many immobilized enzymes [7 ,2 7 ]. The fixed position of immobilized enzyme on the surface of the carrier is thought to prevent extensive distortion of the native conformat ...
The rotary mechanism of the ATP synthase Archives - iGRAD
... [21]; the amino terminal half of d by NMR [22]; and the amino terminal domain of d in complex with a peptide representing the amino terminal region of a subunit [16,23]. Even though we can piece together these fragments to generate a useful model, there are many reasons to obtain structures of entir ...
... [21]; the amino terminal half of d by NMR [22]; and the amino terminal domain of d in complex with a peptide representing the amino terminal region of a subunit [16,23]. Even though we can piece together these fragments to generate a useful model, there are many reasons to obtain structures of entir ...
Plant purple acid phosphatases — genes, structures and biological
... non-PAPs yeast, bacterial or human metallophosphoesterases with exonuclease or phosphodiesterase activity. This group is evolutionarily distinct from typical large and small PAPs and its biochemical and enzymatic properties are unknown. Out of twenty four PAPs sequences of A. thaliana, eighteen cDNA ...
... non-PAPs yeast, bacterial or human metallophosphoesterases with exonuclease or phosphodiesterase activity. This group is evolutionarily distinct from typical large and small PAPs and its biochemical and enzymatic properties are unknown. Out of twenty four PAPs sequences of A. thaliana, eighteen cDNA ...
Comparison of Free Total Amino Acid Compositions and
... difficult for storage and transportation due to their crisp and tender texture. Therefore, they are commercially popular for the local markets in Yunnan Province. On the other hand, Boletus bicolor (Bbi), Boletus speciosus (Bsp), Boletus sinicus (Bsi), Boletus craspedius (Bcr), Boletus griseus (Bgr) ...
... difficult for storage and transportation due to their crisp and tender texture. Therefore, they are commercially popular for the local markets in Yunnan Province. On the other hand, Boletus bicolor (Bbi), Boletus speciosus (Bsp), Boletus sinicus (Bsi), Boletus craspedius (Bcr), Boletus griseus (Bgr) ...
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor
... regulate the SSK2-PBS2-HOG1 mitogen-activated protein kinase cascade (7). In plants, there are several proteins with sequence similarity to histidine kinases, including phytochromes (8) and hormone receptors for ethylene (9 –12) and cytokinins (13). The cytokinin signal transduction pathway has been ...
... regulate the SSK2-PBS2-HOG1 mitogen-activated protein kinase cascade (7). In plants, there are several proteins with sequence similarity to histidine kinases, including phytochromes (8) and hormone receptors for ethylene (9 –12) and cytokinins (13). The cytokinin signal transduction pathway has been ...
Fatty Acid Synthesis
... Formation of a double bond in a fatty acid involves the following endoplasmic reticulum membrane proteins in mammalian cells: NADH-cyt b5 Reductase, a flavoprotein with FAD as prosthetic group. Cytochrome b5, which may be a separate protein or a domain at one end of the desaturase. Desaturase, ...
... Formation of a double bond in a fatty acid involves the following endoplasmic reticulum membrane proteins in mammalian cells: NADH-cyt b5 Reductase, a flavoprotein with FAD as prosthetic group. Cytochrome b5, which may be a separate protein or a domain at one end of the desaturase. Desaturase, ...
PRACTICE SET 6 - UC Davis Plant Sciences
... 1. The hydroxy acid given below can be completely oxidized to acetyl-CoA by betaoxidation. Write the series of individual reactions that will accomplish this degradation. Then write the overall sum showing how much ATP, CoASH and NAD+ is required and how much NADH, acetyl-CoA, etc., are produced on ...
... 1. The hydroxy acid given below can be completely oxidized to acetyl-CoA by betaoxidation. Write the series of individual reactions that will accomplish this degradation. Then write the overall sum showing how much ATP, CoASH and NAD+ is required and how much NADH, acetyl-CoA, etc., are produced on ...
Tasks for 1stMidtermExam. Introduction. Metabolic Regulation
... D. absence of water in the active site 12. The E.coli pyruvic acid dehydrogenase complex is reported to A. decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 B. Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2. C. retard the reduction of pyruvic acid to acetyl Co A and CO2 ...
... D. absence of water in the active site 12. The E.coli pyruvic acid dehydrogenase complex is reported to A. decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 B. Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2. C. retard the reduction of pyruvic acid to acetyl Co A and CO2 ...
27.1 Digestion of Proteins 27.2 Amino Acid Metabolism: An
... from a cascade of inflammatory responses. Understanding the many possible causes of the crystal formation is far from complete. Uric acid is an end product of the breakdown of purine nucleosides. Loss of its acidic H gives urate ion. Anything that increases the production of uric acid or inhibits it ...
... from a cascade of inflammatory responses. Understanding the many possible causes of the crystal formation is far from complete. Uric acid is an end product of the breakdown of purine nucleosides. Loss of its acidic H gives urate ion. Anything that increases the production of uric acid or inhibits it ...
Conserved Tryptophan Residues within Putative Transmembrane
... and Hagenbuch, 2009; Miyagawa et al., 2009; Li et al., 2012). Studies of single nucleotide polymorphisms have also pointed out that mutants located within TMs often result in functional changes (Kalliokoski and Niemi, 2009). In the present study, we used site-directed mutagenesis to study the involv ...
... and Hagenbuch, 2009; Miyagawa et al., 2009; Li et al., 2012). Studies of single nucleotide polymorphisms have also pointed out that mutants located within TMs often result in functional changes (Kalliokoski and Niemi, 2009). In the present study, we used site-directed mutagenesis to study the involv ...
Glycolysis
... It is a common motif for an enzyme active site to be located at an interface between protein domains that are connected by a flexible hinge region. The structural flexibility allows access to the active site, while permitting precise positioning of active site residues, and in some cases exclusion o ...
... It is a common motif for an enzyme active site to be located at an interface between protein domains that are connected by a flexible hinge region. The structural flexibility allows access to the active site, while permitting precise positioning of active site residues, and in some cases exclusion o ...
Problem-Set Solutions
... 26.12 Enzymes and regulatory hormones have higher turnover rates than other proteins. 26.13 Nitrogen balance is the state that results when the amount of nitrogen taken into the body as protein equals the amount of nitrogen excreted from the body in waste material. In a positive nitrogen balance, ni ...
... 26.12 Enzymes and regulatory hormones have higher turnover rates than other proteins. 26.13 Nitrogen balance is the state that results when the amount of nitrogen taken into the body as protein equals the amount of nitrogen excreted from the body in waste material. In a positive nitrogen balance, ni ...
Catalytic triad
A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.