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bio_ch08
bio_ch08

... – Nucleotides pair with one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
8.4 Transcription KEY CONCEPT Transcription converts a gene into a single-stranded RNA molecule.
8.4 Transcription KEY CONCEPT Transcription converts a gene into a single-stranded RNA molecule.

... – Nucleotides pair with one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
8.4 Transcription
8.4 Transcription

... – Nucleotides pair with one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. DNA ...
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Chapter 4 Test Outline - Conackamack Middle School

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... ___ 1.Which of the following terms does not describe DNA replication? a. uni-lateral c. bi-directional b. semi-discontinuous d. semi-conservative ___ 2. The central dogma of molecular biology states that the information in DNA is copied to _______ by the process of __________, and then to __________ ...
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Fundamentals of Nucleic Acid Biochemistry: RNA
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... Splicing reactions (removal of intronic sequences) Polyadenylation (addition of the 3´ polyA tail) ...
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... • Explain why gene expression control is necessary in a eukaryotic cell? • Describe how expression is regulated in before & during transcription? • Tell me what differentiation is? Euchromatin? A silencer sequence? • Explain how gene expression regulation is different in eukaryotes/prokaryotes? ...
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... Describe Beadle and Tatum's Neurospora experiments and their contribution to our understanding of how genes control metabolism. ...
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... Organisms have evolved many ways to protect their DNA from changes. In spite of these mechanisms, however, changes in the ________ occasionally do occur Any change in DNA sequence is called a _____________________. Mutations can be caused by errors in replication, transcription, cell division, or by ...
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... What makes RNA so powerful? • It can fold up • Some of its bases can catalyze chemical reactions • It is highly specific (i.e., complementary) tRNA ...
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... Has 100 potential splicing sites. Could produce 38,000 different polypeptides Many of these polypeptides have been found ...
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... The transcription cycle. The transcription cycle can be described in six steps: (1) Template binding and closed RNA polymerase-promoter complex formation: RNAP binds to DNA and then locates a promoter (P), (2) Open promoter complex formation: once bound to the promoter, RNAP melts the two DNA strand ...
Biology 303 EXAM II 3/14/00 NAME
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... stranded cDNA. Biotin-labeled cRNA produced by in vitro transcription of double-stranded cDNAs using Custom MessageAmp II-Biotin (Ambion, Austin, TX) were fragmented into strands 35–200 bases long. A hybridization cocktail including fragmented cRNAs was first subjected to 99°C for 5 minutes of denat ...
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Supplementary Information (doc 38K)
Supplementary Information (doc 38K)

... To determine the critical region in the GADD45 promoter for BRCA1-dependent activation, a mixture of 1 g of reporter plasmid pGADD45-Luc that contained the human GADD45 promoter region (-107 to +144 or -62 to +144), 2 g of pCMV-BRCA1, and 1 g of pRL-SV40 was transfected into p53-/- cells. The ...
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Transcription - My Teacher Pages

... Once the entire gene has been transcribed, the RNA strand detaches completely from the DNA. Exactly how RNA polymerase recognizes the end of a gene is very complicated but we will discuss as it reaching a Stop signal. ...
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Polyadenylation



Polyadenylation is the addition of a poly(A) tail to a messenger RNA The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.The process of polyadenylation begins as the transcription of a gene finishes, or terminates. The 3'-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3' end. In some genes, these proteins may add a poly(A) tail at any one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing.The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded. However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. This is also sometimes the case for eukaryotic non-coding RNAs.mRNA molecules in both prokaryotes and eukaryotes have polyadenylated 3'-ends, with the prokaryotic poly(A) tails generally shorter and less mRNA molecules polyadenylated.
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