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RNA notes 2015 - OG
RNA notes 2015 - OG

... chain called a ____________________ • Finally, polypeptides fold into various types of proteins and there you have it! ...
Chapter 4: Cellular metabolism
Chapter 4: Cellular metabolism

Chapt16_lecture
Chapt16_lecture

... • Chromatin structure is directly related to the control of gene expression. • Chromatin structure begins with the organization of the DNA into nucleosomes. • Nucleosomes may block RNA polymerase II from gaining access to promoters. ...
Genetics Review
Genetics Review

... The DNA triplets help code for amino acids during translation because DNA is in control of the triplets of mRNA (the codon). The anticodon of the tRNA matches this codon on the ribosome and brings with it an amino acid. ...
NATIONAL BRAIN RESEARCH CENTRE(NBRC) NH-8, Manesar-122050, HARYANA
NATIONAL BRAIN RESEARCH CENTRE(NBRC) NH-8, Manesar-122050, HARYANA

... Mohr’s salt is prepared in warm distilled water by the reaction of (NH4)2SO4 and: ...
DNA
DNA

From Gene to Protein Chapter Questions 7) Which of the following
From Gene to Protein Chapter Questions 7) Which of the following

... 19) A particular eukaryotic protein is 300 amino acids long. Which of the following could be the maximum number of nucleotides in the DNA that codes for the amino acids in this protein? A) 3 B) 100 C) 300 D) 900 E) 1,800 20) A codon A) consists of two nucleotides. B) may code for the same amino acid ...
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Slide 1

... • From code to protein • Protein Synthesis ...
Biology and computers - Cal State LA
Biology and computers - Cal State LA

... how Clustal W gives you a clue as to which part(s) of the Cytochrome C protein you would hypothesize are most important to its function (which is/are the same in all 3 organisms). Start your paragraph as a hypothesis as to which parts are most important, and write your discussion as a defense of you ...
this lecture as PDF here
this lecture as PDF here

... genotypes and compare the observable properties (phenotypes) of the parents with those of the progeny. Bacteria do not mate (in the usual way), so there is no way of getting all the chromosomes of two different bacteria into the same cell. However, there are a number of ways in which a part of the c ...
DNA to Proteins
DNA to Proteins

... • The original strand serves as a template for the new strand ...
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order

... ii. splicing reactions remove introns (noncoding regions) and connect exons. small nuclear RNAs (snRNPs) are involved and form a lariat loop structure to take out the intron and join the exons. 14. T/F: The addition of the polyA tail at the 3’ end of the transcript occurs when the RNA polymerase rea ...
Transcription and Translation
Transcription and Translation

... Transcription: chain termination • Most known about bacterial chain termination • Termination is signaled by a sequence that can form a hairpin loop. • The polymerase and the new RNA molecule are released upon formation of the loop. • Review the transcription animation. ...
DNA YOUTUBE CLIPS
DNA YOUTUBE CLIPS

... 1. mRNA code is “read” – every three bases represents a codon or a triplet – each codon = 1 amino acid – start codon is AUG; anything before the AUG sequence is junk. ...
Transcription in Bacteria
Transcription in Bacteria

... If the gene transcribed encodes a protein, the result of transcription is messenger RNA (mRNA), which then will be used to create that protein via the process of translation. Alternatively, the transcribed gene may encode for either noncoding RNA genes (such as microRNA) or ribosomal RNA (rRNA) or t ...
protein synthesis
protein synthesis

... - Only 1 side of DNA is used - Called Messenger RNA (mRNA) (Note: There are 3 kinds of RNA transcribed in the nucleus; Messenger, Transfer and Ribosomal. Transfer and Ribosomal are stable, and reused so it is mostly mRNA that is being continuously transcribed) 3. The mRNA then moves out through the ...
Engineering of E.coli for production of poly(3
Engineering of E.coli for production of poly(3

...  the microbial P3HP production from glycerol was improved greatly by constructing a genetically stable E.coli recombinant strain  To overcome the instability due to plasmid loss, two strategies were tested and combined ...
Transcription and Translation
Transcription and Translation

... G A A G C U G C U A C U U C G A A C U mRNA copyright cmassengale ...
DNA, RNA and Protein
DNA, RNA and Protein

... • Sugar-phosphate groups are on the outside as a “backbone” • Bases are arranged like rungs on a ladder, perpendicular to the “backbone” • 10 base pairs per turn of the helix ...
Available - Guru Ghasidas Vishwavidyalaya
Available - Guru Ghasidas Vishwavidyalaya

... The plot has two distinct stages, corresponding to deprotonation of two different groups on glycine. At very low pH, the predominant ionic species of glycine is the fully protonated. At the midpoint in the first stage of the titration, in which the –COOH group of glycine loses its proton, equimolar ...
Chapter 21 (part 1) - University of Nevada, Reno
Chapter 21 (part 1) - University of Nevada, Reno

... • Requires unwinding of DNA and synthesis of primer. • Conformational change occurs after DNA binding of RNA polymerase holo-enzyme. • First RNA Polymerase binds to DNA (closed-complex), then conformational change in the polymerase (open complex) causes formation of transcription bubble ...
Transcription in Prokaryotes
Transcription in Prokaryotes

... function e. distance between –10 and –35 regions important f. strength of promoter mostly determined by affinity of RNA pol for promoter DNA sequences g. region unwound by pol appears to be between –9 and +3 (includes right end of –10 seq. and extending to just downstream of transcription initiation ...
Part II: How are antibiotics discovered
Part II: How are antibiotics discovered

E. coli
E. coli

... Unlike eukaryotic systems where transcription and translation occur sequentially, in E. coli, transcription and translation occur simultaneously within the cell In vitro E. coli translation systems are thus performed the same way, coupled, in the same tube under the same reaction conditions. During ...
Gene Expression
Gene Expression

... • All cells in the human body have the same DNA and the same set of genes, yet different cells look different and do different jobs. • Cells have systems to regulate which genes are “turned on” (transcribed) and which are not. ...
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Polyadenylation



Polyadenylation is the addition of a poly(A) tail to a messenger RNA The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.The process of polyadenylation begins as the transcription of a gene finishes, or terminates. The 3'-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3' end. In some genes, these proteins may add a poly(A) tail at any one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing.The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded. However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. This is also sometimes the case for eukaryotic non-coding RNAs.mRNA molecules in both prokaryotes and eukaryotes have polyadenylated 3'-ends, with the prokaryotic poly(A) tails generally shorter and less mRNA molecules polyadenylated.
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