Thymine and Uracil Catabolism in Escherichia coli
... Identical experiments with [2J4C]uracil (Fig. 3) showed that uracil was also catabolized by Escherichia coli K-I2ST-U- in a medium where glutamate was used as principal nitrogen source. At the same time there was no degradation when NH4+was also added to the medium. The rate of uracil breakdown was ...
... Identical experiments with [2J4C]uracil (Fig. 3) showed that uracil was also catabolized by Escherichia coli K-I2ST-U- in a medium where glutamate was used as principal nitrogen source. At the same time there was no degradation when NH4+was also added to the medium. The rate of uracil breakdown was ...
Thuy`s Rho family GTPases review
... • It is found that a gradient of activated Rac is distributed from the front to the rear of migrating cells. • Rac and Rho activitity might be localized to opposite ends of the cell. • Both papers by Buchsbaum and Wittmann indicates that activation of Rac results in down-regulation/inhibition of Rho ...
... • It is found that a gradient of activated Rac is distributed from the front to the rear of migrating cells. • Rac and Rho activitity might be localized to opposite ends of the cell. • Both papers by Buchsbaum and Wittmann indicates that activation of Rac results in down-regulation/inhibition of Rho ...
Production of Chemokines, Interleukin-8 and
... ( S O % ) nor neutrophil (>95%) populations induced the production of either IL-8 or MCP-I, indicating leukocyte specificity for the response. Furthermore, in experiments using transwell plates to separate the two cell populations, no increased expression of chemokine was observed (data not shown), ...
... ( S O % ) nor neutrophil (>95%) populations induced the production of either IL-8 or MCP-I, indicating leukocyte specificity for the response. Furthermore, in experiments using transwell plates to separate the two cell populations, no increased expression of chemokine was observed (data not shown), ...
Induction of light emission by luminescent bacteria treated with UV
... Introduction Light-emitting bacteria are the most abundant and widespread of luminescent organisms (MEIGHEN 1994). Most species of such bacteria live in marine environments (NEALSON 1978). It is well established that luminescent bacteria emit light effectively only when they are at high cell density ...
... Introduction Light-emitting bacteria are the most abundant and widespread of luminescent organisms (MEIGHEN 1994). Most species of such bacteria live in marine environments (NEALSON 1978). It is well established that luminescent bacteria emit light effectively only when they are at high cell density ...
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... of the underlaying material. In addition, biofilm is a potential health risk to humans. Therefore, microorganism growth is not only an issue on Earth but also in manned space habitats like the International Space Station (ISS). The aim of the study was to identify physiological processes relevant fo ...
... of the underlaying material. In addition, biofilm is a potential health risk to humans. Therefore, microorganism growth is not only an issue on Earth but also in manned space habitats like the International Space Station (ISS). The aim of the study was to identify physiological processes relevant fo ...
Influence of organic matter quality in the
... attached and free-living bacterial communities, there are only a few data comparing their kinetics of polymer hydrolysis (Agis et al., 1998). A large fraction of the particulate matter present in marine systems consists of macroscopic aggregates also known as ‘marine snow’ (Alldredge and Silver, 198 ...
... attached and free-living bacterial communities, there are only a few data comparing their kinetics of polymer hydrolysis (Agis et al., 1998). A large fraction of the particulate matter present in marine systems consists of macroscopic aggregates also known as ‘marine snow’ (Alldredge and Silver, 198 ...
Glutamate Controls the Induction of GABA
... To test the role of AMPA receptors in GDPs induction, the selective AMPA– kainate receptor antagonist CNQX (10 – 40 mM) was applied (n 5 11). As reported earlier (Gaiarsa et al. 1991), this compound completely blocked spontaneous GDPs (data not shown), even in rats as young as P2. A full recovery wa ...
... To test the role of AMPA receptors in GDPs induction, the selective AMPA– kainate receptor antagonist CNQX (10 – 40 mM) was applied (n 5 11). As reported earlier (Gaiarsa et al. 1991), this compound completely blocked spontaneous GDPs (data not shown), even in rats as young as P2. A full recovery wa ...
Small Signaling Peptides in Arabidopsis Development
... proliferation of cells in low-density plant cell culture, but it was unclear which unknown substance(s) within the extracts was active in promoting the proliferation. A bioassay based on the mitotic activity of asparagus (Asparagus officinalis) mesophyll cells in liquid culture identified an active, g ...
... proliferation of cells in low-density plant cell culture, but it was unclear which unknown substance(s) within the extracts was active in promoting the proliferation. A bioassay based on the mitotic activity of asparagus (Asparagus officinalis) mesophyll cells in liquid culture identified an active, g ...
Quorum sensing, communication and cross
... (AQs), long-chain fatty acids and fatty acid methyl esters as well as autoinducer-2 (AI-2), a collective term for a group of interconvertible furanones derived from dihydroxypentanedione (DPD) (Fig. 1). AI-2 is also produced by some Gram-positive bacteria, although generally these organisms prefer l ...
... (AQs), long-chain fatty acids and fatty acid methyl esters as well as autoinducer-2 (AI-2), a collective term for a group of interconvertible furanones derived from dihydroxypentanedione (DPD) (Fig. 1). AI-2 is also produced by some Gram-positive bacteria, although generally these organisms prefer l ...
Identity of epibiotic bacteria on symbiontid
... co-evolutionary history between these two sets of partners. This hypothesis is supported by congruent tree topologies inferred from 18S and 16S rDNA from the hosts and bacterial epibionts, respectively. The eukaryotic hosts likely serve as a motile substrate that delivers the epibionts to the ideal ...
... co-evolutionary history between these two sets of partners. This hypothesis is supported by congruent tree topologies inferred from 18S and 16S rDNA from the hosts and bacterial epibionts, respectively. The eukaryotic hosts likely serve as a motile substrate that delivers the epibionts to the ideal ...
Running title: Mode of Bactericidal Action of the Jatropha curcas
... design (CRD) was used to analyse the data and the means were compared with Duncan's Multiple Range test. The differences were considered significant when the p value was ...
... design (CRD) was used to analyse the data and the means were compared with Duncan's Multiple Range test. The differences were considered significant when the p value was ...
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... 2.4 Determination of Minimum Fungicide Concentration (MFC) The in vitro fungicidal activity (MFC) was determined as described by Díaz-Dellavalle et al. 2011. For MFC determination we used a subsample collected from the microplates previously incubated for 72 hs for fungal development in media broth ...
... 2.4 Determination of Minimum Fungicide Concentration (MFC) The in vitro fungicidal activity (MFC) was determined as described by Díaz-Dellavalle et al. 2011. For MFC determination we used a subsample collected from the microplates previously incubated for 72 hs for fungal development in media broth ...
Viability and Cytotoxicity Assay Kits for Diverse Cell Types
... well contained. Subsequent treatment with 4% glutaraldehyde (or less effectively with formaldehyde) permits safer handling during analysis, without disrupting the distinctive staining pattern. Glutaraldehyde is known to inactivate cells and viruses, while preserving their overall morphology. In addi ...
... well contained. Subsequent treatment with 4% glutaraldehyde (or less effectively with formaldehyde) permits safer handling during analysis, without disrupting the distinctive staining pattern. Glutaraldehyde is known to inactivate cells and viruses, while preserving their overall morphology. In addi ...
Facultad de Ciencias Tráfico de células dendríticas a través
... that is responsible for the normal maintenance of fluid balance in tissue. Besides, it is devoted to the transport of fat from the gut and of immune cells from tissues to the lymph nodes. Up to 50% of the protein that extravasates from blood vessels cannot be directly reabsorbed by these capillaries ...
... that is responsible for the normal maintenance of fluid balance in tissue. Besides, it is devoted to the transport of fat from the gut and of immune cells from tissues to the lymph nodes. Up to 50% of the protein that extravasates from blood vessels cannot be directly reabsorbed by these capillaries ...
Effects of Single Amino Acid Substitution on the Biophysical
... substitutions. It is noteworthy that, the i → i + 3 and i → i + 4 hydrophobic interactions were not changed upon the amino acid substitutions at this location; in contrast, the i → i + 3 and i → i + 4 charge interactions were altered by introducing Glu and Lys (Figure 1). Single amino acid substitut ...
... substitutions. It is noteworthy that, the i → i + 3 and i → i + 4 hydrophobic interactions were not changed upon the amino acid substitutions at this location; in contrast, the i → i + 3 and i → i + 4 charge interactions were altered by introducing Glu and Lys (Figure 1). Single amino acid substitut ...
FEMS Microbiology Ecology
... putida strain, strain WCS358, and the R. leguminosarum strain appeared to produce signalling molecules which were recognized by the A. tumefaciens induction machinery (de Weger, unpublished results). This inter-species signalling challenges our traditional view of microbial life in the rhizosphere. ...
... putida strain, strain WCS358, and the R. leguminosarum strain appeared to produce signalling molecules which were recognized by the A. tumefaciens induction machinery (de Weger, unpublished results). This inter-species signalling challenges our traditional view of microbial life in the rhizosphere. ...
Inhibition Systems Mechanisms and Biological Roles of
... chromosome(s) of the indicated bacterial pathogens are shown. Gaps between loci indicate that they are not closely linked. The order of gene regions and orientation of genes is as in the published genomic sequences, except for E. coli 536 and Yersinia pseudotuberculosis PB1þ, which have been reverse ...
... chromosome(s) of the indicated bacterial pathogens are shown. Gaps between loci indicate that they are not closely linked. The order of gene regions and orientation of genes is as in the published genomic sequences, except for E. coli 536 and Yersinia pseudotuberculosis PB1þ, which have been reverse ...
STUDIES ON THE MECHANISM OF THE PHOTOSENSITIZED
... Since the methylene blue absorption spectrum is altered in the presence of the bacteria, it can be assumed that such a change is produced by the ionic binding of the dye on the cell surface. The bond formation between the dye and cell structure shifts the absorption peak toward the shorter wave leng ...
... Since the methylene blue absorption spectrum is altered in the presence of the bacteria, it can be assumed that such a change is produced by the ionic binding of the dye on the cell surface. The bond formation between the dye and cell structure shifts the absorption peak toward the shorter wave leng ...
Life above the boiling point of water?
... observations that (a) maximal and optimal growth temlopes composed of protein subunits which cover their peratures of Staphylothermus marinus are about 7 ° C cytoplasmic membranes ( f i g . 4 ) \ Some coccoid (fig. 5) lower in minimal medium than in full medium and (b) and rod-shaped isolates (fig. ...
... observations that (a) maximal and optimal growth temlopes composed of protein subunits which cover their peratures of Staphylothermus marinus are about 7 ° C cytoplasmic membranes ( f i g . 4 ) \ Some coccoid (fig. 5) lower in minimal medium than in full medium and (b) and rod-shaped isolates (fig. ...
gauze bandages with a bound antimicrobial polymer suppress
... Figure 5 shows the Lower Extremity Graft Sites treated with BIOGUARD gauze bandages. It is evident that although there is a large amount of exudate present, the dressings are not green in color and, based on the observational input, are odor free. Dressing prior to photo: Silvadene or Bacitracin wit ...
... Figure 5 shows the Lower Extremity Graft Sites treated with BIOGUARD gauze bandages. It is evident that although there is a large amount of exudate present, the dressings are not green in color and, based on the observational input, are odor free. Dressing prior to photo: Silvadene or Bacitracin wit ...
Defect in neutrophil killing and increased
... mammalian and insect PGRPs have C-terminal PGRP domains that are highly conserved from insects to mammals (have up to 69% conserved identity and up to 83% conserved similarity between insects and mammals).6 The remaining N-terminal portions of PGRP molecules, however, have very little homology withi ...
... mammalian and insect PGRPs have C-terminal PGRP domains that are highly conserved from insects to mammals (have up to 69% conserved identity and up to 83% conserved similarity between insects and mammals).6 The remaining N-terminal portions of PGRP molecules, however, have very little homology withi ...
Competition with pathogens in mucosal colonization
... cells re-suspended in Eagle MEM. Paradoxically, the intensity of the adherence rate was higher when live bacterial cultures were used, as compared to bacterial washed sediments, meaning that the intensity of adherence to the cellular substrate is depending on the gradient of some bacterial compounds ...
... cells re-suspended in Eagle MEM. Paradoxically, the intensity of the adherence rate was higher when live bacterial cultures were used, as compared to bacterial washed sediments, meaning that the intensity of adherence to the cellular substrate is depending on the gradient of some bacterial compounds ...
Decontamination of the BD FACSAria System
... for contamination. The sheath tank is removable and autoclavable. Some of the tubing is now made from Teflon®, and the new fluidics system requires fewer valves. Valves are a manifold style (with less dead volume than the original style) and are very reliable. The sheath fluid path has a dedicated f ...
... for contamination. The sheath tank is removable and autoclavable. Some of the tubing is now made from Teflon®, and the new fluidics system requires fewer valves. Valves are a manifold style (with less dead volume than the original style) and are very reliable. The sheath fluid path has a dedicated f ...
to Light and Abscisic Acid
... Department of Life Science (S.-O.E., Y.L.), School of Environmental Engineering (Y.L.), Pohang University of Science and Technology, Pohang, 790-784, Korea; and lnstitute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan 11 529, Republic of China (Y.L.) actin monomers and tubulin dimers ...
... Department of Life Science (S.-O.E., Y.L.), School of Environmental Engineering (Y.L.), Pohang University of Science and Technology, Pohang, 790-784, Korea; and lnstitute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan 11 529, Republic of China (Y.L.) actin monomers and tubulin dimers ...
On the natural selection and evolution of the
... confusion) about an appropriate common name to describe them as a group. For example, the appellations quasi-photosynthetic bacteria, erythrobacteria, photosynthetic rhizobia or aerobic anoxygenic phototrophic ...
... confusion) about an appropriate common name to describe them as a group. For example, the appellations quasi-photosynthetic bacteria, erythrobacteria, photosynthetic rhizobia or aerobic anoxygenic phototrophic ...
Chemotaxis
Chemotaxis (from chemo- + taxis) is the movement of an organism in response to a chemical stimulus. Somatic cells, bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). In multicellular organisms, chemotaxis is critical to early development (e.g., movement of sperm towards the egg during fertilization) and subsequent phases of development (e.g., migration of neurons or lymphocytes) as well as in normal function. In addition, it has been recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis.Positive chemotaxis occurs if the movement is toward a higher concentration of the chemical in question; negative chemotaxis if the movement is in the opposite direction. Chemically prompted kinesis (randomly directed or nondirectional) can be called chemokinesis.