Amino acid substitutions that specifically impair the transcriptional
... specific to the E2 TAD or one shared with other transactivation domains, we performed affinity chromatography with the acidic activation domains of herpes simplex (HSV) VP16 and of the tumor suppressor protein p53. These proteins were purified from bacteria as fusions with GST (Fig. 3B), and immobil ...
... specific to the E2 TAD or one shared with other transactivation domains, we performed affinity chromatography with the acidic activation domains of herpes simplex (HSV) VP16 and of the tumor suppressor protein p53. These proteins were purified from bacteria as fusions with GST (Fig. 3B), and immobil ...
Cholinergic modulation of synaptic properties of cortical layer VI
... Cholinergic modulation of cortical input to PoM 463 junction potential (15 mV for the used pipette solution; as calculated in pCLAMP) and the average firing threshold for the recorded cells was −34 mV. To evoke excitatory postsynaptic potentials (EPSPs), 5 electrical pulses (200 µs duration) at 20 ...
... Cholinergic modulation of cortical input to PoM 463 junction potential (15 mV for the used pipette solution; as calculated in pCLAMP) and the average firing threshold for the recorded cells was −34 mV. To evoke excitatory postsynaptic potentials (EPSPs), 5 electrical pulses (200 µs duration) at 20 ...
biography: edwin cohn
... helped to develop a blood fractionation process, which separates the different proteins in blood plasma. This separation of human blood plasma components offered valuable medical treatments. Specifically, the fraction of serum albumin helped to save patients who were in shock during the war. Serum a ...
... helped to develop a blood fractionation process, which separates the different proteins in blood plasma. This separation of human blood plasma components offered valuable medical treatments. Specifically, the fraction of serum albumin helped to save patients who were in shock during the war. Serum a ...
Protist Presentation (to prepare for mini
... First kingdom of eukaryotes Unicellular Nuclei Organelles Reproduce by mitosis Multiple chromosomes Protists often have a very complicated internal structure: a single cell must do all the functions that we have many different cell types to do. ...
... First kingdom of eukaryotes Unicellular Nuclei Organelles Reproduce by mitosis Multiple chromosomes Protists often have a very complicated internal structure: a single cell must do all the functions that we have many different cell types to do. ...
Dm1-MMP, a Matrix Metalloproteinase from Drosophila with a
... cDNA encodes a protein of 541 residues that has a domain organization identical to that of most vertebrate MMPs including a signal sequence, a prodomain with the activation locus, a catalytic domain with a zincbinding site, and a COOH-terminal hemopexin domain. Northern blot analysis of Dm1-MMP expr ...
... cDNA encodes a protein of 541 residues that has a domain organization identical to that of most vertebrate MMPs including a signal sequence, a prodomain with the activation locus, a catalytic domain with a zincbinding site, and a COOH-terminal hemopexin domain. Northern blot analysis of Dm1-MMP expr ...
Microtubules and the Evolution of Mitosis
... It was later shown that MreB, a bacterial protein with homology to actin, is required for the maintenance of cell polarity during asymmetric cell division (Gitai et al. 2004), and is, therefore, also functionally equivalent to its eukaryotic counterpart. Eventually, CreS, a coiled-coiled protein, wh ...
... It was later shown that MreB, a bacterial protein with homology to actin, is required for the maintenance of cell polarity during asymmetric cell division (Gitai et al. 2004), and is, therefore, also functionally equivalent to its eukaryotic counterpart. Eventually, CreS, a coiled-coiled protein, wh ...
PDF
... esterification of amino acids to the 3′ acceptor end of cognate tRNAs (Ibba and Soll, 2000). Cells generally count with a set of twenty aaRSs, each one specific for its cognate amino acid. According to structural and conservation criteria, aaRSs are partitioned in two classes with distinct phylogene ...
... esterification of amino acids to the 3′ acceptor end of cognate tRNAs (Ibba and Soll, 2000). Cells generally count with a set of twenty aaRSs, each one specific for its cognate amino acid. According to structural and conservation criteria, aaRSs are partitioned in two classes with distinct phylogene ...
Calcium, a signaling molecule in the endoplasmic reticulum?
... in the lumen of the ER. Overall, changes in the free [Ca21]ER might result in cycles of Ca21-dependent association and dissociation, and this is important because it might facilitate the passage of certain newly synthesized proteins from one chaperone to another during the folding process. ...
... in the lumen of the ER. Overall, changes in the free [Ca21]ER might result in cycles of Ca21-dependent association and dissociation, and this is important because it might facilitate the passage of certain newly synthesized proteins from one chaperone to another during the folding process. ...
Alu repeats as transcriptional regulatory platforms in macrophage
... subtypes. These contained several transcription factor binding sites, including those for members of the MEF2 and ATF families, and LXR and RAR nuclear receptors, all of which have been implicated in macrophage differentiation, survival, and responses to stress and infection. Combining bioinformatic ...
... subtypes. These contained several transcription factor binding sites, including those for members of the MEF2 and ATF families, and LXR and RAR nuclear receptors, all of which have been implicated in macrophage differentiation, survival, and responses to stress and infection. Combining bioinformatic ...
in Thymocytes and Mature T Cells Transduction Pathways to Induce
... not splenic T cell apoptosis. A, Thymocytes and splenic T cells were treated with 10⫺7 M Dex in the absence or presence of 100 M pancaspase (D/P), caspase-3 (D/3), caspase-8 (D/8), or caspase-9 (D/9) inhibitor for 20 h. Culture in the presence of vehicle alone (0.5% DMSO) served as control. Relativ ...
... not splenic T cell apoptosis. A, Thymocytes and splenic T cells were treated with 10⫺7 M Dex in the absence or presence of 100 M pancaspase (D/P), caspase-3 (D/3), caspase-8 (D/8), or caspase-9 (D/9) inhibitor for 20 h. Culture in the presence of vehicle alone (0.5% DMSO) served as control. Relativ ...
Investigation of a Zα-like Peptide Motif in Koi Herpesvirus
... with Zα domains. Therefore, circular dichroism (CD) spectra were collected of the wild type (WT), Y84A mutant, and N62D mutant ORF112 proteins titrated into poly(dGdC) and calf thymus B-DNA. The WT and Y84A mutant proteins bound to two distinct DNA conformations that did not indicate a B- to Z-DNA t ...
... with Zα domains. Therefore, circular dichroism (CD) spectra were collected of the wild type (WT), Y84A mutant, and N62D mutant ORF112 proteins titrated into poly(dGdC) and calf thymus B-DNA. The WT and Y84A mutant proteins bound to two distinct DNA conformations that did not indicate a B- to Z-DNA t ...
From Molecular Machines to Hierarchical Modeling
... that carefully recognize particular compounds and move them into and out of the cell. In Synechococcus WH8102, there are about eighty genes that encode for ABC transporters, including about eighteen specific to substrate-binding proteins. The regulation of transport is a complex multi-level process. ...
... that carefully recognize particular compounds and move them into and out of the cell. In Synechococcus WH8102, there are about eighty genes that encode for ABC transporters, including about eighteen specific to substrate-binding proteins. The regulation of transport is a complex multi-level process. ...
S1 Topic 8 The Basic Structure of a Cell
... students understand the meanings. Where necessary, further diagrams should be used as illustration. ...
... students understand the meanings. Where necessary, further diagrams should be used as illustration. ...
the molecular basis of dehydration tolerance in plants
... example, a cDNA-encoding glyceraldehyde-3-phosphate dehydrogenase, isolated from the resurrection plant C. plantagineum (Table 1; 129), shows increased expression during drought and upon ABA treatment. However, increased levels of the enzyme are also associated with other environmental stresses in p ...
... example, a cDNA-encoding glyceraldehyde-3-phosphate dehydrogenase, isolated from the resurrection plant C. plantagineum (Table 1; 129), shows increased expression during drought and upon ABA treatment. However, increased levels of the enzyme are also associated with other environmental stresses in p ...
34750 - Radboud Repository
... Mitochondria are eukaryotic organelles that originated from the endosymbiosis of an alpha-proteobacterium. To gain insight into the evolution of the mitochondrial proteome as it proceeded through the transition from a free-living cell to a specialized organelle, we compared a reconstructed ancestral ...
... Mitochondria are eukaryotic organelles that originated from the endosymbiosis of an alpha-proteobacterium. To gain insight into the evolution of the mitochondrial proteome as it proceeded through the transition from a free-living cell to a specialized organelle, we compared a reconstructed ancestral ...
Apyrase Suppression Raises Extracellular ATP
... seedlings when apyrase expression is suppressed were assayed by microarray and quantitative real-time-PCR analyses. The most significant gene expression changes induced by APY suppression were in genes involved in biotic stress responses, which include those genes regulating wall composition and exte ...
... seedlings when apyrase expression is suppressed were assayed by microarray and quantitative real-time-PCR analyses. The most significant gene expression changes induced by APY suppression were in genes involved in biotic stress responses, which include those genes regulating wall composition and exte ...
Plant serine/arginine-rich proteins and their role in pre
... one of these proteins (UBP) in protoplasts enhanced the splicing of pre-mRNAs that are otherwise inefficiently processed. The importance of the GC content in exons in efficient splicing, and an AG-rich exonic element in promoting downstream 5 0 splice site selection, has also been reported in some c ...
... one of these proteins (UBP) in protoplasts enhanced the splicing of pre-mRNAs that are otherwise inefficiently processed. The importance of the GC content in exons in efficient splicing, and an AG-rich exonic element in promoting downstream 5 0 splice site selection, has also been reported in some c ...
Nucleases in Bdellovibrio bacteriovorus contribute towards efficient
... Bdellovibrio bacteriovorus are predatory bacteria that burrow into prey bacteria and degrade their cell contents, including DNA and RNA, to grow. Their genome encodes diverse nucleases, some with potential export sequences. Transcriptomic analysis determined two candidate-predicted nuclease genes (b ...
... Bdellovibrio bacteriovorus are predatory bacteria that burrow into prey bacteria and degrade their cell contents, including DNA and RNA, to grow. Their genome encodes diverse nucleases, some with potential export sequences. Transcriptomic analysis determined two candidate-predicted nuclease genes (b ...
Lectures 1 and 2
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
Poster
... β-catenin is a multi-functional protein involved in two essential cellular pathways: cell-cell adhesion and transcriptional regulation8. β-catenin contains 12 armadillo repeats capped by a C-helix. An amino acid important for β-catenin’s electrostatic interactions with ligands is Lys-435 known as th ...
... β-catenin is a multi-functional protein involved in two essential cellular pathways: cell-cell adhesion and transcriptional regulation8. β-catenin contains 12 armadillo repeats capped by a C-helix. An amino acid important for β-catenin’s electrostatic interactions with ligands is Lys-435 known as th ...
The lipid phosphatase LPP3 regulates extra
... receptors (Chun et al., 2002)], G protein coupled transmembrane receptors (Fukushima and Chun, 2001; Hla, 2001). Activation of these receptors enhances adhesion, migration and morphogenesis of capillary endothelial cells, as well as inhibiting T-cell apoptosis. However, the mitogenic effects of phos ...
... receptors (Chun et al., 2002)], G protein coupled transmembrane receptors (Fukushima and Chun, 2001; Hla, 2001). Activation of these receptors enhances adhesion, migration and morphogenesis of capillary endothelial cells, as well as inhibiting T-cell apoptosis. However, the mitogenic effects of phos ...
HueyLing - Principles of Bioseparations.pmd
... extraction where the solute is transferred from a liquid to another with which the original solvent is immiscible. 1.6.5. Solute-solute separation in liquid medium Solute-solute separation is by far the most challenging form of separation. An example of this is the separation of serum albumin from o ...
... extraction where the solute is transferred from a liquid to another with which the original solvent is immiscible. 1.6.5. Solute-solute separation in liquid medium Solute-solute separation is by far the most challenging form of separation. An example of this is the separation of serum albumin from o ...
reviews - Lab. Biodados UFMG
... re-enter a quiescent state and can undergo enforced differentiation32–34. Deletion of Rb in the skin results in a decrease in the number of label-retaining, slowly cycling cells in the stem cell compartment35. These data raise two different possibilities: first, RB-mutant skin stem cells might die o ...
... re-enter a quiescent state and can undergo enforced differentiation32–34. Deletion of Rb in the skin results in a decrease in the number of label-retaining, slowly cycling cells in the stem cell compartment35. These data raise two different possibilities: first, RB-mutant skin stem cells might die o ...
Root-knot nematodes manipulate plant cell functions during a
... implicated in feeding cell formation. AtCEL1 has been shown to be upregulated in developing giant cells (Mitchum et al., 2004). All members of the class A and class B expansin superfamily regulated upon Meloidogyne infestation have been shown to be activated in A. thaliana (Jammes et al., 2005). LeE ...
... implicated in feeding cell formation. AtCEL1 has been shown to be upregulated in developing giant cells (Mitchum et al., 2004). All members of the class A and class B expansin superfamily regulated upon Meloidogyne infestation have been shown to be activated in A. thaliana (Jammes et al., 2005). LeE ...
Characterization of the Plasmid-Encoded Arsenic Salts Resistance
... ArsR of R773, the pair of Cys-32 and Cys-34 is conserved and an important component of the metal recognition site. However, there was only one Cys-33 in the putative metalbinding domain of ArsR from K. oxytoca. Therefore, we presumed that Ser-31 and Cys-33 could be the ligands to arsenate, which res ...
... ArsR of R773, the pair of Cys-32 and Cys-34 is conserved and an important component of the metal recognition site. However, there was only one Cys-33 in the putative metalbinding domain of ArsR from K. oxytoca. Therefore, we presumed that Ser-31 and Cys-33 could be the ligands to arsenate, which res ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.