Strategies of actin reorganisation in plant cells
... tissues and led to a better understanding of F-actin organisation during plant development and morphogenesis (Mathur et al., 1999; Fu et al., 2001; Ilgenfritz et al., 2003; Ketelaar et al., 2004; Weerasinghe et al., 2005). This probe, however, exhibited a number of side effects (Ketelaar et al., 200 ...
... tissues and led to a better understanding of F-actin organisation during plant development and morphogenesis (Mathur et al., 1999; Fu et al., 2001; Ilgenfritz et al., 2003; Ketelaar et al., 2004; Weerasinghe et al., 2005). This probe, however, exhibited a number of side effects (Ketelaar et al., 200 ...
Full Text - The International Journal of Developmental Biology
... As evo-devo questions go, tracing the evolutionary history of gastrulation is particularly difficult, because the complex morphogenetic and fate specification events involved make gastrulation an emergent property of development. Gastrulation has undoubtedly undergone significant modification in ext ...
... As evo-devo questions go, tracing the evolutionary history of gastrulation is particularly difficult, because the complex morphogenetic and fate specification events involved make gastrulation an emergent property of development. Gastrulation has undoubtedly undergone significant modification in ext ...
Characterization of the unconventional myosin VIII in plant cells and
... 1166) (Knight and Kendrick-Jones, 1993) which has no apparent homologies or recognisable motifs. The only noticeable features are two clusters of serine residues and a cluster of basic residues at its very C-terminus. For expression, a vector of the pGEX series was modi®ed so that fusion proteins wi ...
... 1166) (Knight and Kendrick-Jones, 1993) which has no apparent homologies or recognisable motifs. The only noticeable features are two clusters of serine residues and a cluster of basic residues at its very C-terminus. For expression, a vector of the pGEX series was modi®ed so that fusion proteins wi ...
Biggins
... the same experiment and found similar results: After 40 min at the nonpermissive temperature, 10% of the GALMPS1 cells had cytokinesed compared with 40% of the GAL-MPS1 ipl1-321 cells (data not shown). To determine whether the strains expressed different levels of Mps1 protein, we analyzed Mps1–myc ...
... the same experiment and found similar results: After 40 min at the nonpermissive temperature, 10% of the GALMPS1 cells had cytokinesed compared with 40% of the GAL-MPS1 ipl1-321 cells (data not shown). To determine whether the strains expressed different levels of Mps1 protein, we analyzed Mps1–myc ...
Aldosterone in Australia and Mineralocorticoids in
... aldosterone, the mineralocorticoid receptor (MR), is a ligand-binding domain (LBD). The modular nature of the ligand-dependent transcription factor that is a member of steroid receptors enables one to 'mix and match'. To the nuclear receptor superfamily (1). In collaboration with understand the mole ...
... aldosterone, the mineralocorticoid receptor (MR), is a ligand-binding domain (LBD). The modular nature of the ligand-dependent transcription factor that is a member of steroid receptors enables one to 'mix and match'. To the nuclear receptor superfamily (1). In collaboration with understand the mole ...
MyRIP, a novel Rab effector, enables myosin VIIa recruitment to
... types where myosin VIIa has been detected (Hasson et al., 1995; El-Amraoui et al., 1996). In the photoreceptor cells, MyRIP was localized in the synaptic region. Ultrastructural analysis of MyRIP localization showed that the protein was present in both pre- and post-synaptic areas (data not shown). ...
... types where myosin VIIa has been detected (Hasson et al., 1995; El-Amraoui et al., 1996). In the photoreceptor cells, MyRIP was localized in the synaptic region. Ultrastructural analysis of MyRIP localization showed that the protein was present in both pre- and post-synaptic areas (data not shown). ...
IBCApplication_Revised02032015
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
UVA radiation causes DNA strand breaks, chromosomal
... Although the photon energy may be insufficient to damage DNA directly, UVA radiation is able to penetrate into the epidermis down to the proliferative basal cells and even further to the dermal compartment where it contributes to photoaging. In addition, evidence is increasing that UVA may also be re ...
... Although the photon energy may be insufficient to damage DNA directly, UVA radiation is able to penetrate into the epidermis down to the proliferative basal cells and even further to the dermal compartment where it contributes to photoaging. In addition, evidence is increasing that UVA may also be re ...
Mesenchymal Stem Cells: New Approaches for the Treatment of
... cells. The wide availability of adipose tissue from liposuction procedures also means that adipose-derived MSCs are more practical to study in the laboratory setting. In most academic medical centers, a steady stream of adipose tissue is usually available from various plastic surgical and some neuro ...
... cells. The wide availability of adipose tissue from liposuction procedures also means that adipose-derived MSCs are more practical to study in the laboratory setting. In most academic medical centers, a steady stream of adipose tissue is usually available from various plastic surgical and some neuro ...
Severe osmotic compression triggers a slowdown of
... association rates of several proteins inside the cell. Molecular crowding has received little attention in living cells, although it is usually invoked to explain why biochemical reactions rates may vary in vivo and in vitro (26–29). For instance, the diffusion coefficient of the green fluorescent pro ...
... association rates of several proteins inside the cell. Molecular crowding has received little attention in living cells, although it is usually invoked to explain why biochemical reactions rates may vary in vivo and in vitro (26–29). For instance, the diffusion coefficient of the green fluorescent pro ...
Characterization and Evaluation of a Novel Tissue
... Scaffolds for engineered heart valves must function immediately after implantation, but must also permit repopulation with autologous host cells and facilitate gradual remodeling. Native aortic heart valves are composed of three layers, i.e. two strong external fibrous layers (ventricularis and fibr ...
... Scaffolds for engineered heart valves must function immediately after implantation, but must also permit repopulation with autologous host cells and facilitate gradual remodeling. Native aortic heart valves are composed of three layers, i.e. two strong external fibrous layers (ventricularis and fibr ...
Boya
... In this Gram-stained smear of a positive blood culture bottle one can see gram-pos cocci in pairs, chains (arrows), and clusters (arrowhead). It is often possible to diff entiate streptococci (chains) from staphylococci (clusters). When they are seen tog however, it is best to remain non-committal, ...
... In this Gram-stained smear of a positive blood culture bottle one can see gram-pos cocci in pairs, chains (arrows), and clusters (arrowhead). It is often possible to diff entiate streptococci (chains) from staphylococci (clusters). When they are seen tog however, it is best to remain non-committal, ...
![WOX4 Promotes Procambial Development1[W][OA]](http://s1.studyres.com/store/data/015871676_1-3651be0b9530e879640c6e2c082fe002-300x300.png)
WOX4 Promotes Procambial Development1[W][OA]
... Plant shoot organs arise from initial cells that are recruited from meristematic tissues. Previous studies have shown that members of the WUSCHEL-related HOMEOBOX (WOX) gene family function to organize various initial cell populations during plant development. The function of the WOX4 gene is previo ...
... Plant shoot organs arise from initial cells that are recruited from meristematic tissues. Previous studies have shown that members of the WUSCHEL-related HOMEOBOX (WOX) gene family function to organize various initial cell populations during plant development. The function of the WOX4 gene is previo ...
ANTHER AND POLLEN CULTURE
... CULTURE MEDIUM:The anther culture medium requirements vary with genotype and probably the age of the anther as well as condition under which donor plants are grown. In corporation of activated charcol into the medium has stimulated the induction of androgenesis. The iron in the medium plays a very i ...
... CULTURE MEDIUM:The anther culture medium requirements vary with genotype and probably the age of the anther as well as condition under which donor plants are grown. In corporation of activated charcol into the medium has stimulated the induction of androgenesis. The iron in the medium plays a very i ...
Direct killing of Epstein-Barr virus (EBV)–infected B
... (HLA) class I peptide tetramers has highlighted the large proportion of CD8 T cells directed to EBV antigens.7,8 These CD8⫹ CTL responses are preferentially directed toward the early lytic proteins, BZLF1 (BamH fragment Z left frame 1) and BMLF1 (BamHI-M leftward reading frame 1)9-12 and to a lesser ...
... (HLA) class I peptide tetramers has highlighted the large proportion of CD8 T cells directed to EBV antigens.7,8 These CD8⫹ CTL responses are preferentially directed toward the early lytic proteins, BZLF1 (BamH fragment Z left frame 1) and BMLF1 (BamHI-M leftward reading frame 1)9-12 and to a lesser ...
LAB 2: ASEPTIC TECHNIQUE AND TRANSFER OF
... Fig. 2. Isolation and identification of anaerobes Specimens should be free of contaminating bacteria. Material from sites that are normally sterile, such as blood, spinal fluid, or pleural fluid, poses no problem provided the usual precautions are taken to decontaminate the skin properly before pun ...
... Fig. 2. Isolation and identification of anaerobes Specimens should be free of contaminating bacteria. Material from sites that are normally sterile, such as blood, spinal fluid, or pleural fluid, poses no problem provided the usual precautions are taken to decontaminate the skin properly before pun ...
Role of - Molecular Physiology and Biophysics
... dot) on unstimulated Schwann cells may represent single a-DG molecule, as suggested previously for muscle cells (28). When Schwann cells were challenged with rLNa2G-coated M. leprae (after removing free rLNa2G), a-DG labeling on most of the Schwann cells appeared as aggregates or macroclusters with ...
... dot) on unstimulated Schwann cells may represent single a-DG molecule, as suggested previously for muscle cells (28). When Schwann cells were challenged with rLNa2G-coated M. leprae (after removing free rLNa2G), a-DG labeling on most of the Schwann cells appeared as aggregates or macroclusters with ...
Vps41 Phosphorylation and the Rab Ypt7 Control the Targeting of
... sensitive to Ypt7 inhibitors than wild-type vacuoles in the in vitro vacuole fusion reaction. If Yck3 is overproduced or cells are challenged by osmotic shock, Vps41 phosphorylation is clearly observed in vivo. In addition, we observed a faster recovery of in vivo fusion in yck3⌬ cells under highly ...
... sensitive to Ypt7 inhibitors than wild-type vacuoles in the in vitro vacuole fusion reaction. If Yck3 is overproduced or cells are challenged by osmotic shock, Vps41 phosphorylation is clearly observed in vivo. In addition, we observed a faster recovery of in vivo fusion in yck3⌬ cells under highly ...
University of Colorado at Boulder
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
Interplay between cell growth and cell cycle in
... an atypical E2F family member (E2Fe; DEL1: DP-E2F-like 1) promotes mitotic cycles by repressing aFZR homologue called CCS52A2 (Vlieghe et al., 2005; Lammens et al., 2008). Higher ploidy correlates with increased cell size both at the whole-organism level and at the level of individual cells within ...
... an atypical E2F family member (E2Fe; DEL1: DP-E2F-like 1) promotes mitotic cycles by repressing aFZR homologue called CCS52A2 (Vlieghe et al., 2005; Lammens et al., 2008). Higher ploidy correlates with increased cell size both at the whole-organism level and at the level of individual cells within ...
Supplemental Figure Legends
... experiments each performed in duplicate). b, Mitochondrial pore opening in Ppif+/+ and Ppif-/MEFs treated as in a. Following H2O2 exposure, MEFs were stained with calcein-AM in the presence of CoCl2, which results in selective staining of mitochondria that is imaged by confocal microscopy. Pore open ...
... experiments each performed in duplicate). b, Mitochondrial pore opening in Ppif+/+ and Ppif-/MEFs treated as in a. Following H2O2 exposure, MEFs were stained with calcein-AM in the presence of CoCl2, which results in selective staining of mitochondria that is imaged by confocal microscopy. Pore open ...
μMACS™ mRNA Isolation Kits
... and washing steps. The μMACS mRNA Isolation is a robust and reproducible procedure based on MACS® Technology. It enables direct isolation of mRNA without prior preparation of total RNA. With the μMACS mRNA Isolation Kits full length, intact mRNA can be obtained from fresh, frozen, or cultured cells1 ...
... and washing steps. The μMACS mRNA Isolation is a robust and reproducible procedure based on MACS® Technology. It enables direct isolation of mRNA without prior preparation of total RNA. With the μMACS mRNA Isolation Kits full length, intact mRNA can be obtained from fresh, frozen, or cultured cells1 ...
plant cell biology in the new millennium: new tools and new
... its conception by Marvin Minsky in the 1950s, confocal microscopy has now become a routine technique and indispensable tool not only for cell biological studies but also for molecular investigations (Lichtman, 1994). In addition, although used by only a handful of research laboratories worldwide sin ...
... its conception by Marvin Minsky in the 1950s, confocal microscopy has now become a routine technique and indispensable tool not only for cell biological studies but also for molecular investigations (Lichtman, 1994). In addition, although used by only a handful of research laboratories worldwide sin ...
Ligand Maintenance of Regulatory T Cells via GITR for B Cells in
... Recently, the role of B cells in autoimmune diseases has been further studied by their depletion using anti-CD20, which targets B cells from the pre-B cell to memory stages. Plasma cells, which do not express CD20, are not eliminated. In EAE, anti-CD20 depletion of B cells before induction of EAE wi ...
... Recently, the role of B cells in autoimmune diseases has been further studied by their depletion using anti-CD20, which targets B cells from the pre-B cell to memory stages. Plasma cells, which do not express CD20, are not eliminated. In EAE, anti-CD20 depletion of B cells before induction of EAE wi ...
How plant cells explore geometry - The polarity induction and
... Cell polarity and axis development are central for plant morphogenesis. Cell movement as central mechanism for animal morphogenesis does not play a role in the walled cells of higher plants. Plant development rather relies on flexible alignment of cell axis adjusting cellular differentiation with re ...
... Cell polarity and axis development are central for plant morphogenesis. Cell movement as central mechanism for animal morphogenesis does not play a role in the walled cells of higher plants. Plant development rather relies on flexible alignment of cell axis adjusting cellular differentiation with re ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.