441_2009_904_MOESM1_ESM - Springer Static Content Server
... identified by moderate HCN1 and weak NOS staining). A weak HCN3 expression is also seen in cells with IF13 (dark purple arrow, identified by strong NOS and no HCN1). c-panels: The triple staining for HCN1 (green, HCN1), HCN3 (red, HCN3 Shigemoto), and vilip1 (blue, vilip1) shows the strong express ...
... identified by moderate HCN1 and weak NOS staining). A weak HCN3 expression is also seen in cells with IF13 (dark purple arrow, identified by strong NOS and no HCN1). c-panels: The triple staining for HCN1 (green, HCN1), HCN3 (red, HCN3 Shigemoto), and vilip1 (blue, vilip1) shows the strong express ...
The membrane form of the DNA repair protein Ku interacts at the cell
... family (Hartley et al, 1995). Extensive genetic and molecular studies have identified the DNA-PK complex as an integral component of mammalian DNA DSB repair pathway (Jackson, 2002). Accordingly, mice with deletions of Ku genes are hypersensitive to ionizing radiation and lack mature lymphocytes (for ...
... family (Hartley et al, 1995). Extensive genetic and molecular studies have identified the DNA-PK complex as an integral component of mammalian DNA DSB repair pathway (Jackson, 2002). Accordingly, mice with deletions of Ku genes are hypersensitive to ionizing radiation and lack mature lymphocytes (for ...
Aprotinin Preserves Cellular Junctions and Reduces
... myocardial edema attributable to vascular permeability, which is regulated in part by thrombin-induced alterations in cellular junctions. Aprotinin has been demonstrated to prevent activation of the thrombin protease-activated receptor, and we hypothesized that aprotinin preserves myocardial cellula ...
... myocardial edema attributable to vascular permeability, which is regulated in part by thrombin-induced alterations in cellular junctions. Aprotinin has been demonstrated to prevent activation of the thrombin protease-activated receptor, and we hypothesized that aprotinin preserves myocardial cellula ...
PHYSIOLOGICAL AND CHEMICAL CHARACTERISTICS OF
... antibacterial activity. The concentration and composition of electrolytes as well as the pH are regulated by a variety of ion pumps and channels that maintain conditions maximizing the antimicrobial activity. A similar regulation system was found in the cystic fibrosis (CF) syndrome where the homeos ...
... antibacterial activity. The concentration and composition of electrolytes as well as the pH are regulated by a variety of ion pumps and channels that maintain conditions maximizing the antimicrobial activity. A similar regulation system was found in the cystic fibrosis (CF) syndrome where the homeos ...
Epithelial-Mesenchymal Transition in Pancreatic Carcinoma
... models [34], it is likely that the SMAD4 status defines two subclasses of pancreatic carcinoma with very distinct behavior. In tumors with functional SMAD4, the cytostatic effects of TGF have to be prevented by other mechanisms (e.g., hyperactive Ras signaling, overexpression of Ski, etc.), and thu ...
... models [34], it is likely that the SMAD4 status defines two subclasses of pancreatic carcinoma with very distinct behavior. In tumors with functional SMAD4, the cytostatic effects of TGF have to be prevented by other mechanisms (e.g., hyperactive Ras signaling, overexpression of Ski, etc.), and thu ...
Patterns of Nogo mRNA and Protein Expression in the Developing
... (mAb), IN-1, raised against a major myelin-associated neurite growth inhibitor, called NI-250, was able to neutralize the growth-inhibiting activities of the substrate to the extent that neurons in culture could send out neurites on a CNS myelin substrate or on CNS white matter (Caroni and Schwab, 1 ...
... (mAb), IN-1, raised against a major myelin-associated neurite growth inhibitor, called NI-250, was able to neutralize the growth-inhibiting activities of the substrate to the extent that neurons in culture could send out neurites on a CNS myelin substrate or on CNS white matter (Caroni and Schwab, 1 ...
Experimental conditions affect the site of tetrazolium
... been established in bacteria. The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate test with growing cells, and a liquid ...
... been established in bacteria. The objective of the present study was to identify the reduction mechanisms of tetrazolium violet (TV) in Lactococcus lactis using a mutagenesis approach, under two experimental conditions generally applied in microbiology: a plate test with growing cells, and a liquid ...
18. THYROID FUNCTION
... Figure 18-3. General aspects of follicular activity The thyroglobulin is continuously emptied into the center of the follicle where it forms the colloid. This mode continues until the follicle becomes distended and filled with colloid. Once a follicle is filled the cells surrounding that follicle pr ...
... Figure 18-3. General aspects of follicular activity The thyroglobulin is continuously emptied into the center of the follicle where it forms the colloid. This mode continues until the follicle becomes distended and filled with colloid. Once a follicle is filled the cells surrounding that follicle pr ...
MAIT Cells Precursor Derivatives Activating Mouse Derived Riboflavin
... wild-type copy of these genes in trans on an expression plasmid. Therefore, BW25113 (pKD46) cells also contained one of the plasmids pMLD419, pMLD420, pMLD421, pMLD424, or pMLD422 to allow inactivation of chromosomal genes ribA, ribB, ribC, ribD, or ribE, respectively. In all cases, chloramphenicol- ...
... wild-type copy of these genes in trans on an expression plasmid. Therefore, BW25113 (pKD46) cells also contained one of the plasmids pMLD419, pMLD420, pMLD421, pMLD424, or pMLD422 to allow inactivation of chromosomal genes ribA, ribB, ribC, ribD, or ribE, respectively. In all cases, chloramphenicol- ...
BE WHEN THEY GROW UP? Lessons from Epidermal Patterning in
... Plants, like other multicellular eukaryotes, develop from a single-celled zygote that ultimately gives rise to the many specialized cell types of the adult organism. Cell patterning is when cells are guided to their appropriate differentiated fate at the correct time and place in the developing orga ...
... Plants, like other multicellular eukaryotes, develop from a single-celled zygote that ultimately gives rise to the many specialized cell types of the adult organism. Cell patterning is when cells are guided to their appropriate differentiated fate at the correct time and place in the developing orga ...
Lymphatic vessels and tertiary lymphoid organs
... lymphatic outflow that then gives rise to chronic inflammation at the local site (45). They suggest that defective lymphatic drainage is a prerequisite for the development of TLOs and have provided data supporting this concept in chronic graft rejection. Once the TLO has developed, the LVs may funct ...
... lymphatic outflow that then gives rise to chronic inflammation at the local site (45). They suggest that defective lymphatic drainage is a prerequisite for the development of TLOs and have provided data supporting this concept in chronic graft rejection. Once the TLO has developed, the LVs may funct ...
The many ways to age for a single yeast cell
... replicative ageing mirrors senescence under proliferative conditions and may also model the ageing process of mitotically active cells in higher eukaryotes, including human stem cell populations. Notably, in recent years technically more refined and automated methods have been developed to remove dau ...
... replicative ageing mirrors senescence under proliferative conditions and may also model the ageing process of mitotically active cells in higher eukaryotes, including human stem cell populations. Notably, in recent years technically more refined and automated methods have been developed to remove dau ...
From segment to somite: Segmentation to
... are undetermined with respect to their developmental pathway. At the level of the determination front (depicted by the posterior-most dotted presumptive segment), the interaction of the clock and gradient specifies the chemical prepattern. Cells of the PSM that lie anterior to the determination front ...
... are undetermined with respect to their developmental pathway. At the level of the determination front (depicted by the posterior-most dotted presumptive segment), the interaction of the clock and gradient specifies the chemical prepattern. Cells of the PSM that lie anterior to the determination front ...
Chemotherapy Targets the Hair-Follicle Vascular
... chemotherapy drug, induces disruption of the hair cycle and subsequent alopecia. We show in this report that doxorubicin causes disruption of the hair-follicle-associated blood vessel network resulting in a greatly reduced density of these blood vessels. Dystrophic hair follicles were also observed ...
... chemotherapy drug, induces disruption of the hair cycle and subsequent alopecia. We show in this report that doxorubicin causes disruption of the hair-follicle-associated blood vessel network resulting in a greatly reduced density of these blood vessels. Dystrophic hair follicles were also observed ...
Phospholipase D Activation Correlates with Microtubule
... In some cases, evidence for PA stimulating the downstream responses also was provided (Munnik, 2001). In general, PA is thought to work by binding target proteins downstream in the signaling cascade. Binding may activate these components directly or indirectly by concentrating them at membrane loci ...
... In some cases, evidence for PA stimulating the downstream responses also was provided (Munnik, 2001). In general, PA is thought to work by binding target proteins downstream in the signaling cascade. Binding may activate these components directly or indirectly by concentrating them at membrane loci ...
Controls T Cell Differentiation RAGE Ligation Affects T Cell
... It has also been suggested that RAGE ligation may affect adaptive immune responses, but the role of this pattern recognition receptor on T cells has not been well studied, and some investigations have failed to show a role of RAGE in adaptive responses (11). These studies and previous studies indica ...
... It has also been suggested that RAGE ligation may affect adaptive immune responses, but the role of this pattern recognition receptor on T cells has not been well studied, and some investigations have failed to show a role of RAGE in adaptive responses (11). These studies and previous studies indica ...
Pulmonary immune cells in health and disease: lymphocytes
... human T-cell subset<; are known. In most T-cells, TCR is constituted by two distinct transmembrane heterodimers formed by « and ~ binding chains [14]; more recently, it has been demonstrated that a small subset of T-cells expresses y/o heterodimers [ 15]. Using WT31 MoAb that recognizes the w~ TCR, ...
... human T-cell subset<; are known. In most T-cells, TCR is constituted by two distinct transmembrane heterodimers formed by « and ~ binding chains [14]; more recently, it has been demonstrated that a small subset of T-cells expresses y/o heterodimers [ 15]. Using WT31 MoAb that recognizes the w~ TCR, ...
Axis-inducing activities and cell fates of the zebrafish organizer.
... suggested the possibility that anterior specification in zebrafish, similar to mouse, requires spatially distinct activities. The fact that the embryonic shield of another teleost fish, Perca, is capable of inducing complete secondary axis formation, however, suggests that part of the organizer’s ac ...
... suggested the possibility that anterior specification in zebrafish, similar to mouse, requires spatially distinct activities. The fact that the embryonic shield of another teleost fish, Perca, is capable of inducing complete secondary axis formation, however, suggests that part of the organizer’s ac ...
The Bordetella pertussis adenylate cyclase toxin binds to T cells via
... To further characterize the interaction of CyaA with its putative receptor on T cells, we compared CyaA binding to quiescent and activated T cells. Peripheral T cells were stimulated at different times with an agonistic anti-CD3 mAb, and CyaA binding was measured by flow cytometry. Strikingly, TCR e ...
... To further characterize the interaction of CyaA with its putative receptor on T cells, we compared CyaA binding to quiescent and activated T cells. Peripheral T cells were stimulated at different times with an agonistic anti-CD3 mAb, and CyaA binding was measured by flow cytometry. Strikingly, TCR e ...
Auxin transport through non-hair cells sustains root
... The plant hormone auxin controls root epidermal cell development in a concentration-dependent manner 1-3. Root hairs are produced on a subset of epidermal cells as they increase in distance from the root tip. Auxin is required for their initiation 4-7 and continued growth 8-11, but little is known a ...
... The plant hormone auxin controls root epidermal cell development in a concentration-dependent manner 1-3. Root hairs are produced on a subset of epidermal cells as they increase in distance from the root tip. Auxin is required for their initiation 4-7 and continued growth 8-11, but little is known a ...
Pulsatile Stretch Remodels Cell-to-Cell Communication in Cultured
... PS was associated with a marked and rapid effect on electrical activity. The results recorded in 4 culture dishes from the same batch are illustrated in Figure 2. The top panel of Figure 2 shows the transmembrane action potential upstrokes recorded before PS from 96 sites. The corresponding isochron ...
... PS was associated with a marked and rapid effect on electrical activity. The results recorded in 4 culture dishes from the same batch are illustrated in Figure 2. The top panel of Figure 2 shows the transmembrane action potential upstrokes recorded before PS from 96 sites. The corresponding isochron ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.