Isolation of a novel population of multipotent stem cells
... with PI staining. About 50 µl of cell suspension was aliquoted into vials (10 cells/per vial ) and incubated with 2 µl of the antibodies. Various biomarkers of stem cells such as CD34 – one of the marker’s of skin stem cell, CD 49f & CD29 - common stem cell markers, CD45 Lymphocytic marker, CD90 (Th ...
... with PI staining. About 50 µl of cell suspension was aliquoted into vials (10 cells/per vial ) and incubated with 2 µl of the antibodies. Various biomarkers of stem cells such as CD34 – one of the marker’s of skin stem cell, CD 49f & CD29 - common stem cell markers, CD45 Lymphocytic marker, CD90 (Th ...
Alfy, a novel FYVE-domain-containing protein associated with
... been found to bind to PtdIns(3)P, although a few examples that lack one or more consensus residues bind with lower affinity and also show some affinity for the structurally related phosphoinositide PtdIns(5)P (Sankaran et al., 2001). The Phox-homology (PX) domain is a ~100-residue β-sandwich ...
... been found to bind to PtdIns(3)P, although a few examples that lack one or more consensus residues bind with lower affinity and also show some affinity for the structurally related phosphoinositide PtdIns(5)P (Sankaran et al., 2001). The Phox-homology (PX) domain is a ~100-residue β-sandwich ...
How much territory can a single E. coli cell control?
... S-adenosylmethionine (SAM) metabolism. The first long E. coli isolated in the Newman lab was derived from a supposed metK mutant described in R. C. Greene’s lab (Greene et al., 1973). This carried a point mutation in metK together with an lrp mutation which decreased Lrp expression and increased met ...
... S-adenosylmethionine (SAM) metabolism. The first long E. coli isolated in the Newman lab was derived from a supposed metK mutant described in R. C. Greene’s lab (Greene et al., 1973). This carried a point mutation in metK together with an lrp mutation which decreased Lrp expression and increased met ...
Regulation of Potassium Transport in Leaves: from Molecular to
... In addition to speci®c K+-selective channels, guard cells also possess a wide range of non-selective cation channels (NSCC), either depolarization- or hyperpolarization-activated (Demidchik et al., 2002). These channels are likely to be involved in release of solutes during turgor adjustment and, to ...
... In addition to speci®c K+-selective channels, guard cells also possess a wide range of non-selective cation channels (NSCC), either depolarization- or hyperpolarization-activated (Demidchik et al., 2002). These channels are likely to be involved in release of solutes during turgor adjustment and, to ...
Tumor-specific T cells in human Merkel cell carcinomas: a possible
... itself has induced regression of MCC (Friedlaender et al., 2002; Heath et al., 2008; Muirhead and Ritchie, 2007; Val-Bernal et al., 2011; Wooff et al., 2010). Although 80% of MCCs have genomic integration of MCV and produce viral proteins including small and large T antigens, these tumors are still ...
... itself has induced regression of MCC (Friedlaender et al., 2002; Heath et al., 2008; Muirhead and Ritchie, 2007; Val-Bernal et al., 2011; Wooff et al., 2010). Although 80% of MCCs have genomic integration of MCV and produce viral proteins including small and large T antigens, these tumors are still ...
Immunosuppressants 2
... – Destroys proliferating lymphoid cells (cytotoxic agent) also alkylate some resting cells (Thus, it is very toxic) – Clinical Uses: • Before the discovery of Mycophenolate, cyclophosphamide was the drug of choice for treatment of many autoimmune diseases like SLE; autoimmune hemolytic diseases and ...
... – Destroys proliferating lymphoid cells (cytotoxic agent) also alkylate some resting cells (Thus, it is very toxic) – Clinical Uses: • Before the discovery of Mycophenolate, cyclophosphamide was the drug of choice for treatment of many autoimmune diseases like SLE; autoimmune hemolytic diseases and ...
The alternative oxidase lowers mitochondrial reactive oxygen
... may serve a more general function in all plant species by limiting mitochondrial ROS formation. An experimental basis for this hypothesis is that conditions that induce AOX expression, including chilling (13), pathogen attack (14), aging (15), and inhibition of the cytochrome pathway (8), also cause ...
... may serve a more general function in all plant species by limiting mitochondrial ROS formation. An experimental basis for this hypothesis is that conditions that induce AOX expression, including chilling (13), pathogen attack (14), aging (15), and inhibition of the cytochrome pathway (8), also cause ...
ONLINE METHODS Experiments were conducted in accordance
... vibratome and labeled by using Gene-gun (Bio Rad) and Dil-labeled tungsten particles (1.3µm, Bio Rad). After 24 hours at room temperature, sections were incubated with 5% goat serum in 0.1% PBS-X for 1 hour, then incubated with anti-RGS14 antibody (1/500) in 3% goat serum 0.05% PBS-X for 16 hours, a ...
... vibratome and labeled by using Gene-gun (Bio Rad) and Dil-labeled tungsten particles (1.3µm, Bio Rad). After 24 hours at room temperature, sections were incubated with 5% goat serum in 0.1% PBS-X for 1 hour, then incubated with anti-RGS14 antibody (1/500) in 3% goat serum 0.05% PBS-X for 16 hours, a ...
flow cytometric measurement of reactive oxygen species production
... metals (aluminum) induces a mediated oxidative stressdependent apoptosis. Measurement of reactive oxygen species (ROS) by flow cytometry proves a sensitive method that succeeds in quantify this production inside the cell, with the advantage of an accurate determination, knowing that free radical spe ...
... metals (aluminum) induces a mediated oxidative stressdependent apoptosis. Measurement of reactive oxygen species (ROS) by flow cytometry proves a sensitive method that succeeds in quantify this production inside the cell, with the advantage of an accurate determination, knowing that free radical spe ...
The role of T cells in Osteoporosis - International Journal of Clinical
... T Helper Cells and Osteoclastogenesis Depending on the manner in which they are activated, T cells can mediate both osteoclastogenesis and anti-osteoclastogenesis [32]. The net effect of T cells on osteoclastogenesis depends on the balance between positive and negative factors expressed by the T cel ...
... T Helper Cells and Osteoclastogenesis Depending on the manner in which they are activated, T cells can mediate both osteoclastogenesis and anti-osteoclastogenesis [32]. The net effect of T cells on osteoclastogenesis depends on the balance between positive and negative factors expressed by the T cel ...
Dual-color HIV reporters trace a population of latently infected cells
... SFFV or EF1α promoter. To address this possibility, we treated the double-negative populations with the same drugs (Fig. 2A and B, bottom) and found only very limited reactivation, affecting o1% of the population. This implies that the silent HIV provirus in the double-negative population has little ...
... SFFV or EF1α promoter. To address this possibility, we treated the double-negative populations with the same drugs (Fig. 2A and B, bottom) and found only very limited reactivation, affecting o1% of the population. This implies that the silent HIV provirus in the double-negative population has little ...
Euglena gracilis by Cadmium
... has formed in cells grown under illumination or in the dark. An analogous ZnBP could not be isolated from control or Zn-exposed (20 ,ug/mL) cells, but zinc and a trace of copper were bound to the CdBP when 2-mercaptoethanol (2-ME) is added to the homogenates of Cd-treated cells and the buffers used ...
... has formed in cells grown under illumination or in the dark. An analogous ZnBP could not be isolated from control or Zn-exposed (20 ,ug/mL) cells, but zinc and a trace of copper were bound to the CdBP when 2-mercaptoethanol (2-ME) is added to the homogenates of Cd-treated cells and the buffers used ...
ATP-sensitive potassium channels in capillaries isolated from
... 1998; Frieden et al. 1999) and are able to produce membrane potential oscillations (Usachev et al. 1995). Many of the functions of the endothelium, for example, the release of vasoactive compounds and the regulation of the permeability of the vascular wall, are influenced by the free intracellular c ...
... 1998; Frieden et al. 1999) and are able to produce membrane potential oscillations (Usachev et al. 1995). Many of the functions of the endothelium, for example, the release of vasoactive compounds and the regulation of the permeability of the vascular wall, are influenced by the free intracellular c ...
An antibody raised to a maize auxin-binding protein has inhibitory
... The inhibition of occurrence of first divísion only affects a fraction of the protoplast population, this fraction being about one third of the total population at the highest IgG concentration tested. This is in contrast with the fact that anti-abpl IgGs at a ten fold lower concentration inhibits t ...
... The inhibition of occurrence of first divísion only affects a fraction of the protoplast population, this fraction being about one third of the total population at the highest IgG concentration tested. This is in contrast with the fact that anti-abpl IgGs at a ten fold lower concentration inhibits t ...
The added value of single-cell gene expression
... the differences in expression contributed by subpopulations. There is also risk that the reference genes selected have different expression profiles in existing subpopulations; the Normfinder algorithm does not sense this. Traditional cell population analysis is performed on thousands of cells, some ...
... the differences in expression contributed by subpopulations. There is also risk that the reference genes selected have different expression profiles in existing subpopulations; the Normfinder algorithm does not sense this. Traditional cell population analysis is performed on thousands of cells, some ...
Transport Across Membranes
... • Most Active Transport systems are driven by metabolic energy derived from ATP • Active Transport allows cells to uptake necessary ions and molecules and remove waste products • These Mechanisms often referred to as Pumps • Most common is Sodium Pump ...
... • Most Active Transport systems are driven by metabolic energy derived from ATP • Active Transport allows cells to uptake necessary ions and molecules and remove waste products • These Mechanisms often referred to as Pumps • Most common is Sodium Pump ...
Exosome-Depleted FBS Using Beckman Coulter Centrifugation:
... of the process. In this process, large volumes of FBS can easily be centrifuged at high speeds to eliminate native extracellular vesicles typically in an overnight spin. However, there are also commercially available products which have pre-conditioned the media to be depleted of exosomes and other ...
... of the process. In this process, large volumes of FBS can easily be centrifuged at high speeds to eliminate native extracellular vesicles typically in an overnight spin. However, there are also commercially available products which have pre-conditioned the media to be depleted of exosomes and other ...
Can We Use Nanotechnology to Treat Cancer?
... the nanoparticles on breast cancer cells that we could grow in the laboratory. We tried three different conditions with the breast cancer cells. We had one group of cells that got nanoparticles with medicine but did not get inductive heating, one that got nanoparticles with no medicine in them but t ...
... the nanoparticles on breast cancer cells that we could grow in the laboratory. We tried three different conditions with the breast cancer cells. We had one group of cells that got nanoparticles with medicine but did not get inductive heating, one that got nanoparticles with no medicine in them but t ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.