The Cell - University of South Carolina
... In this example of a nerve cell stained with hematoxylin and eosin, you can see the extent to which you can resolve cell components in the using a light microscope. In this particular nerve cell, the nucleolus is large and the DNA in the nucleus is fully extended in a form that is called euchromatin ...
... In this example of a nerve cell stained with hematoxylin and eosin, you can see the extent to which you can resolve cell components in the using a light microscope. In this particular nerve cell, the nucleolus is large and the DNA in the nucleus is fully extended in a form that is called euchromatin ...
Precision Optogenetic Tool for Selective Single- and Multiple
... Photoablation of Mammalian Cells by miniSOG2 To demonstrate its application in cell ablation, we expressed miniSOG2 in HEK293 cells. To visualize individual cells by fluorescence, we co-expressed histone 2B (H2B)-EGFP fusion protein, which labels the nucleus. The transfected cells were illuminated w ...
... Photoablation of Mammalian Cells by miniSOG2 To demonstrate its application in cell ablation, we expressed miniSOG2 in HEK293 cells. To visualize individual cells by fluorescence, we co-expressed histone 2B (H2B)-EGFP fusion protein, which labels the nucleus. The transfected cells were illuminated w ...
Certain Aspects of Cell Lineage and Morphogenesis
... will therefore escape treatment with ultra-violet light. The micro-beam in all cases was 60 fx in diameter (Plate 1, fig. 1) and was administered for 30 seconds. All these eggs were fixed at 15 hours, sectioned, and examined for gonad and mid-gut structure. By comparison with the controls (as shown ...
... will therefore escape treatment with ultra-violet light. The micro-beam in all cases was 60 fx in diameter (Plate 1, fig. 1) and was administered for 30 seconds. All these eggs were fixed at 15 hours, sectioned, and examined for gonad and mid-gut structure. By comparison with the controls (as shown ...
500KB - NZQA
... lowering activation energy. They are specific to a reaction and are not used up in the reaction. Enzymes are involved in each step of DNA replication. During DNA replication, a specific enzyme unwinds the DNA helix exposing nucleotides. Another enzyme synthesises the new DNA strands by adding comple ...
... lowering activation energy. They are specific to a reaction and are not used up in the reaction. Enzymes are involved in each step of DNA replication. During DNA replication, a specific enzyme unwinds the DNA helix exposing nucleotides. Another enzyme synthesises the new DNA strands by adding comple ...
Stem cells in the light of evolution
... Regeneration has received renewed research interest in recent years. Stem cells have been extensively studied due to their regeneration potential. First, this property was shown by histologists in the 19th century who introduced an abstract term for cells which can specifically repair or regenerat ...
... Regeneration has received renewed research interest in recent years. Stem cells have been extensively studied due to their regeneration potential. First, this property was shown by histologists in the 19th century who introduced an abstract term for cells which can specifically repair or regenerat ...
The zebrafish as a model to study intestinal inflammation
... (Fig. 2). Differentiated epithelial cells such as absorptive enterocytes (anterior and mid-intestine), mucin-producting Goblet cells (entire intestine) and enteroendocrine cells (anterior intestine) are found. To date, Paneth cells are not identified histologically. Also no classical microfold (M) ce ...
... (Fig. 2). Differentiated epithelial cells such as absorptive enterocytes (anterior and mid-intestine), mucin-producting Goblet cells (entire intestine) and enteroendocrine cells (anterior intestine) are found. To date, Paneth cells are not identified histologically. Also no classical microfold (M) ce ...
NCEA Level 2 Biology (91156) 2016
... lowering activation energy. They are specific to a reaction and are not used up in the reaction. Enzymes are involved in each step of DNA replication. During DNA replication, a specific enzyme unwinds the DNA helix exposing nucleotides. Another enzyme synthesises the new DNA strands by adding comple ...
... lowering activation energy. They are specific to a reaction and are not used up in the reaction. Enzymes are involved in each step of DNA replication. During DNA replication, a specific enzyme unwinds the DNA helix exposing nucleotides. Another enzyme synthesises the new DNA strands by adding comple ...
Structure and function of nucleus-vacuole junctions: outer
... alignments revealed four conserved regions in Nvj1p (Fig. 1A,B). Region I at the N-terminus is characterized by a hydrophobic signal anchor-like sequence (Fig. 1B,C). Region II overlaps with the predicted membrane-spanning domain of Nvj1p (Fig. 1B,C). Region III is adjacent to the membranespanning d ...
... alignments revealed four conserved regions in Nvj1p (Fig. 1A,B). Region I at the N-terminus is characterized by a hydrophobic signal anchor-like sequence (Fig. 1B,C). Region II overlaps with the predicted membrane-spanning domain of Nvj1p (Fig. 1B,C). Region III is adjacent to the membranespanning d ...
Ludwig Institute for Cancer Research Brussels Branch
... Cancer is a major concern in human health. The prospects for bringing cancer under control require linked innovative basic and clinical research. In this view, Daniel K. Ludwig created in 1971 the Ludwig Institute for Cancer Research, an international organization bringing together scientists and cl ...
... Cancer is a major concern in human health. The prospects for bringing cancer under control require linked innovative basic and clinical research. In this view, Daniel K. Ludwig created in 1971 the Ludwig Institute for Cancer Research, an international organization bringing together scientists and cl ...
Supplementation of Animal Cell Culture Media
... found suitable for specialized applications, such as mammalian stem cell or insect cell culture. Recently, serum suppliers have provided so-called “fortified sera,” which for identified applications can offer better performance at a lower price. Such products are defined types of serum that have bee ...
... found suitable for specialized applications, such as mammalian stem cell or insect cell culture. Recently, serum suppliers have provided so-called “fortified sera,” which for identified applications can offer better performance at a lower price. Such products are defined types of serum that have bee ...
Regulatory role of rpL3 in cell response to nucleolar stress induced
... subunits to the cytoplasm.4 In growing and proliferating tumor cells, the process of ribosome biogenesis is enhanced compared with normal somatic cells5 suggesting that tumor cells are more sensitive to nucleolar stress than normal cells. Thus, chemotherapeutic agents that selectively target ribosom ...
... subunits to the cytoplasm.4 In growing and proliferating tumor cells, the process of ribosome biogenesis is enhanced compared with normal somatic cells5 suggesting that tumor cells are more sensitive to nucleolar stress than normal cells. Thus, chemotherapeutic agents that selectively target ribosom ...
Shp1 regulates T cell homeostasis by limiting IL
... and CCR7. Together, these findings suggest that the CD4+ memory phenotype compartment contained a mixture of effector and memory phenotype cells. In contrast, the CD8+ CD44hi populations contained a high proportion of CD62Lhi CD127hi CCR7hi cells, suggesting a prominent central memory phenotype. Th ...
... and CCR7. Together, these findings suggest that the CD4+ memory phenotype compartment contained a mixture of effector and memory phenotype cells. In contrast, the CD8+ CD44hi populations contained a high proportion of CD62Lhi CD127hi CCR7hi cells, suggesting a prominent central memory phenotype. Th ...
a57cc3f5fd4205a2bed6190a2df34d87-a
... into irradiated glands they were able to restore submandibular gland morphology and function. Most of the established cell transplantation requires a donor able to provide a sufficient number of cells. A dearth of such donors and the possible immune-reaction against the allogeneic cells are the majo ...
... into irradiated glands they were able to restore submandibular gland morphology and function. Most of the established cell transplantation requires a donor able to provide a sufficient number of cells. A dearth of such donors and the possible immune-reaction against the allogeneic cells are the majo ...
Antigen-antibody complex stimulation of dendritic cells Oscar Díaz
... antigen presentation by DCs 100-fold compared to soluble antigens and additionally, have showed a possible application in cancer therapy. This study shows an increased stimulation of MoDCs by two different antigen-antibody immune complexes. DCs were derived from monocytes isolated from PBMCs and cul ...
... antigen presentation by DCs 100-fold compared to soluble antigens and additionally, have showed a possible application in cancer therapy. This study shows an increased stimulation of MoDCs by two different antigen-antibody immune complexes. DCs were derived from monocytes isolated from PBMCs and cul ...
Evidence for Gal3p`s Cytoplasmic Location
... concentration of 2%. At the indicated time points, an aliquot of cells was removed and processed for ␣-galactosidase enzyme activity assay. Activities are expressed as picomoles of p-nitrophenyl-␣-D-galactopyranoside released per milligram of protein per minute at 30°C. Error bars show standard devi ...
... concentration of 2%. At the indicated time points, an aliquot of cells was removed and processed for ␣-galactosidase enzyme activity assay. Activities are expressed as picomoles of p-nitrophenyl-␣-D-galactopyranoside released per milligram of protein per minute at 30°C. Error bars show standard devi ...
Biology - OpenWetWare
... In 1838, Matthias Schleiden concluded that all plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from division of existing cells. These discoveries led to the cell theory. ...
... In 1838, Matthias Schleiden concluded that all plants were made of cells. In 1839, Theodor Schwann stated that all animals were made of cells. In 1855, Rudolph Virchow concluded that new cells were created only from division of existing cells. These discoveries led to the cell theory. ...
The 2016 Nobel Prize in Physiology or Medicine
... The Nobel Prize in Physiology or Medicine for 2016 was awarded to Yoshinori Ohsumi (Japan) for his work on autophagy. Autophagy, meaning ‘self-eating’, is the process where the cell content like large macromolecule complexes or organelles are fused with the lysosome for degradation and recycling. Wh ...
... The Nobel Prize in Physiology or Medicine for 2016 was awarded to Yoshinori Ohsumi (Japan) for his work on autophagy. Autophagy, meaning ‘self-eating’, is the process where the cell content like large macromolecule complexes or organelles are fused with the lysosome for degradation and recycling. Wh ...
bluebird bio, Inc. - corporate
... Reduced Tonic Signaling and Methods to Enhance Memory T Cells Results presented by Kevin Friedman, Ph.D., bluebird bio. bluebird bio has developed a potent CAR targeting BCMA (bb2121) to treat multiple myeloma and some lymphomas. The initial clinical application of this technology to treat patien ...
... Reduced Tonic Signaling and Methods to Enhance Memory T Cells Results presented by Kevin Friedman, Ph.D., bluebird bio. bluebird bio has developed a potent CAR targeting BCMA (bb2121) to treat multiple myeloma and some lymphomas. The initial clinical application of this technology to treat patien ...
Recent Advances in αβ T Cell Biology: Wnt Signaling
... extracellular inhibitors of Wnt signaling in fetal thymus organ culture induces a complete block of early thymocyte development, indicating that secreted Wnt factors are essential for intrathymic T lineage development [17]. Specifically, expression of Wnt1 and Wnt4 through retroviral transduction yi ...
... extracellular inhibitors of Wnt signaling in fetal thymus organ culture induces a complete block of early thymocyte development, indicating that secreted Wnt factors are essential for intrathymic T lineage development [17]. Specifically, expression of Wnt1 and Wnt4 through retroviral transduction yi ...
Chloroplasts – Structure and Function
... Exam Hint:- Questions about chloroplasts nearly always link them to other aspects of photosynthesis, so make sure you revise the light-dependent and light -independent reactions, action and absorption spectra and factors that affect the rate of photosynthesis. Data interpretation questions involving ...
... Exam Hint:- Questions about chloroplasts nearly always link them to other aspects of photosynthesis, so make sure you revise the light-dependent and light -independent reactions, action and absorption spectra and factors that affect the rate of photosynthesis. Data interpretation questions involving ...
Photolabeling of Proteins and Cells
... concern that newly synthesized molecules will become fluorescent and complicate the results, which can occur when studying protein dynamics by photobleaching methods (15). Furthermore, photoactivation of PAGFP could produce a population of highlighted proteins more rapidly and with greater optical e ...
... concern that newly synthesized molecules will become fluorescent and complicate the results, which can occur when studying protein dynamics by photobleaching methods (15). Furthermore, photoactivation of PAGFP could produce a population of highlighted proteins more rapidly and with greater optical e ...
Retinal pigment epithelial cells phagocytosis of T lymphocytes
... immune system by different mechanisms.1 At the level of the posterior segment of the eye, retinal pigment epithelial (RPE) cells have a crucial role in the induction and maintenance of this immune privilege.2 RPE cells constitute the external blood-retinal barrier, an interface between the retina an ...
... immune system by different mechanisms.1 At the level of the posterior segment of the eye, retinal pigment epithelial (RPE) cells have a crucial role in the induction and maintenance of this immune privilege.2 RPE cells constitute the external blood-retinal barrier, an interface between the retina an ...
Antigen recognition by T Lymphocytes
... Peptide loading on MHC • MHC Class I: intracellular antigens, e.g. viral proteins produced in virus-infected cells; peptide degradation in cytosol by proteasome, then transport to ER • MHC Class II: extracellular antigens, e.g. pathogen engulfed by phagocytes; degradation in phagosome and lysosome ...
... Peptide loading on MHC • MHC Class I: intracellular antigens, e.g. viral proteins produced in virus-infected cells; peptide degradation in cytosol by proteasome, then transport to ER • MHC Class II: extracellular antigens, e.g. pathogen engulfed by phagocytes; degradation in phagosome and lysosome ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.