Engineering amount of cell–cell contact demonstrates biphasic
... used previously facilitated the formation of pairs of cells, it was difficult to form groups of three or more cells with specified arrangements of cell–cell contacts. To overcome this limitation we developed a method which uses dielectrophoretic traps to actively and simultaneously position the cell ...
... used previously facilitated the formation of pairs of cells, it was difficult to form groups of three or more cells with specified arrangements of cell–cell contacts. To overcome this limitation we developed a method which uses dielectrophoretic traps to actively and simultaneously position the cell ...
Cytosolic DNA Triggers Mitochondrial Apoptosis via DNA Damage
... 1) secretion of cytokines that activate the immune system and 2) induction of cell death in infected cells. In the case of viruses and some intracellular bacteria, their nucleic acids act as the principal PAMPs recognized. The retinoid acid-induced gene I (RIG-I)–like helicases (RLHs) recognize cyto ...
... 1) secretion of cytokines that activate the immune system and 2) induction of cell death in infected cells. In the case of viruses and some intracellular bacteria, their nucleic acids act as the principal PAMPs recognized. The retinoid acid-induced gene I (RIG-I)–like helicases (RLHs) recognize cyto ...
L-Carnosine reduces telomere damage and shortening
... nonenzymic glycosylation of proteins [4,7,11]. Many studies have been carried out to investigate the effects of carnosine at the molecular or cellular level. However, its effect on telomere has never been reported. Owing to the involvement of telomere in the replicative senescence of human cells, it w ...
... nonenzymic glycosylation of proteins [4,7,11]. Many studies have been carried out to investigate the effects of carnosine at the molecular or cellular level. However, its effect on telomere has never been reported. Owing to the involvement of telomere in the replicative senescence of human cells, it w ...
Quantitative reverse transcription polymerase chain reaction
... chain reaction (Q-RT-PCR) methods applicable to the environmental study of V. cholerae in VNBC populations and under starvation conditions. Therefore, we selected 16S rRNA, and mRNA of the tufA/B, rpoS and relA genes as molecular targets to explore the utility of the total RNA fraction for culture-i ...
... chain reaction (Q-RT-PCR) methods applicable to the environmental study of V. cholerae in VNBC populations and under starvation conditions. Therefore, we selected 16S rRNA, and mRNA of the tufA/B, rpoS and relA genes as molecular targets to explore the utility of the total RNA fraction for culture-i ...
Intrinsic Cardiac Catecholamines Help Maintain Beating
... (usually six wells per plate) seeded from the same preparation of myocytes. Measurements were obtained immediately before and after the addition of specified drugs. In the reserpine experiment, beating rate measurements were obtained at mul- ...
... (usually six wells per plate) seeded from the same preparation of myocytes. Measurements were obtained immediately before and after the addition of specified drugs. In the reserpine experiment, beating rate measurements were obtained at mul- ...
Foreword
... different cell components that allow us to better understand their role and the implications of changing the relative proportions and structure of these components. E.g. change in hemicellulose or lignin content and its distribution within the cell wall layers. o Validation of cell wall models throu ...
... different cell components that allow us to better understand their role and the implications of changing the relative proportions and structure of these components. E.g. change in hemicellulose or lignin content and its distribution within the cell wall layers. o Validation of cell wall models throu ...
Reference
... one of top priority that must be achieved early on in the project. Moreover, any novel molecules, transcriptional factors or signal transduction pathways found in one sub-project will be supported quickly by researchers working in on other projects in terms of technical help, insight sharing and dis ...
... one of top priority that must be achieved early on in the project. Moreover, any novel molecules, transcriptional factors or signal transduction pathways found in one sub-project will be supported quickly by researchers working in on other projects in terms of technical help, insight sharing and dis ...
Subcellular localization of yeast CDC46 varies with the cell cycle.
... cdc46 cells arrest with their DNA unreplicated, and we infer that DNA elongation is not blocked. More detailed interpretation of the DNA synthesis defect in cdc46 cells is given in the Discussion, below. Among the characterized S. cerevisiae cell division mutants, cdc7, which is essential for DNA in ...
... cdc46 cells arrest with their DNA unreplicated, and we infer that DNA elongation is not blocked. More detailed interpretation of the DNA synthesis defect in cdc46 cells is given in the Discussion, below. Among the characterized S. cerevisiae cell division mutants, cdc7, which is essential for DNA in ...
LC1
... Animal and plant cells have different contents Cell differentiation is needed in all organisms Cell specialisation happens in humans Microscopes have helped us better understand cells The nucleus is crucial in the cell Chromosomes control who you are Cells need to replicate accurately Stem cells sho ...
... Animal and plant cells have different contents Cell differentiation is needed in all organisms Cell specialisation happens in humans Microscopes have helped us better understand cells The nucleus is crucial in the cell Chromosomes control who you are Cells need to replicate accurately Stem cells sho ...
TE project Paper (edited) FINAL
... therefore are somewhat limited in the types of differentiation they can undergo. However, they do exhibit the characteristic of plasticity, meaning that they can differentiate into cells of other tissues. 9 Similar to ES cells, adult stem cells are capable of self-renewal and self-maintenance for lo ...
... therefore are somewhat limited in the types of differentiation they can undergo. However, they do exhibit the characteristic of plasticity, meaning that they can differentiate into cells of other tissues. 9 Similar to ES cells, adult stem cells are capable of self-renewal and self-maintenance for lo ...
Enzymatic Evidence for Differences in the
... Rh(D), (C), and (E) p01ypeptides,8"~as well as those showing variation in their one- and two-dimensional peptide "maps" after radioiodination and pr~teolysis,"~'~~'~ suggest that these antigens are components of similar but not structurally identical proteins. What, if any, relationship these antige ...
... Rh(D), (C), and (E) p01ypeptides,8"~as well as those showing variation in their one- and two-dimensional peptide "maps" after radioiodination and pr~teolysis,"~'~~'~ suggest that these antigens are components of similar but not structurally identical proteins. What, if any, relationship these antige ...
CK12 Passive Transport - Diffusion, Osmosis, and Facilitated Diffusion
... are equally disbursed throughout the area. In other words, there is no longer an area of high concentration or low concentration. This is a natural process and does not require energy because molecules are always in motion. Diffusion can occur across a semipermeable membrane, or a membrane that allo ...
... are equally disbursed throughout the area. In other words, there is no longer an area of high concentration or low concentration. This is a natural process and does not require energy because molecules are always in motion. Diffusion can occur across a semipermeable membrane, or a membrane that allo ...
The molecular basis for selective assembly of the UBAP1
... domain (amino acids 17–63) (Agromayor et al., 2012; de Souza and Aravind, 2010; Stefani et al., 2011). Although we failed to express UBAP1(1-63)–strep (data not shown) a slightly longer Nterminal fragment, UBAP1(1-95)–strep, that incorporates an additional predicted a-helix (Fig. 2B), could be gener ...
... domain (amino acids 17–63) (Agromayor et al., 2012; de Souza and Aravind, 2010; Stefani et al., 2011). Although we failed to express UBAP1(1-63)–strep (data not shown) a slightly longer Nterminal fragment, UBAP1(1-95)–strep, that incorporates an additional predicted a-helix (Fig. 2B), could be gener ...
On diamond surface properties and interactions with neurons.
... Diamond possesses all the characteristics to fulfil these requirements: its chemical inertness, mechanical stiffness, optical transparency from IR to UV and the possibility of functionalizing the surface at the nanoscale by hydrophobic and electrical conductive H termination or hydrophilic and elect ...
... Diamond possesses all the characteristics to fulfil these requirements: its chemical inertness, mechanical stiffness, optical transparency from IR to UV and the possibility of functionalizing the surface at the nanoscale by hydrophobic and electrical conductive H termination or hydrophilic and elect ...
Opportunities to Explore Plant Membrane
... Live-cell imaging adds the dimension of time to data acquisition, enabling the study of molecular dynamics. However, tradeoffs between spatial resolution, temporal resolution, field of view, brightness, and number of frames must be weighed. STED is limited by photodamage (with high-depletion laser i ...
... Live-cell imaging adds the dimension of time to data acquisition, enabling the study of molecular dynamics. However, tradeoffs between spatial resolution, temporal resolution, field of view, brightness, and number of frames must be weighed. STED is limited by photodamage (with high-depletion laser i ...
Recent developments in atomic force microscopy for underwater
... the out of plane modulus. Other factors such as the local density of microfibrils and pectin network status could make more significant contribution to the nanoindentation modulus. Pectin forms a gel phase structure that holds cellulose-hemicelluloses frame in cell walls. From the mechanical point o ...
... the out of plane modulus. Other factors such as the local density of microfibrils and pectin network status could make more significant contribution to the nanoindentation modulus. Pectin forms a gel phase structure that holds cellulose-hemicelluloses frame in cell walls. From the mechanical point o ...
Epigenetic changes and disturbed neural development in a human
... methylation of lysines 4 and 27 of histone H3 were detected in the promoter region of PAX6 and OCT4. The changes in these activating and silencing histone marks provide a more general mechanistic rational for the regulation of developmentally important genes at non-cytotoxic drug concentrations. ...
... methylation of lysines 4 and 27 of histone H3 were detected in the promoter region of PAX6 and OCT4. The changes in these activating and silencing histone marks provide a more general mechanistic rational for the regulation of developmentally important genes at non-cytotoxic drug concentrations. ...
CAR1, a TNFR–Related Protein, Is a Cellular Receptor for
... clone encodes the ALV-B susceptibility factor (data not shown). To identify RNA transcripts encoding this factor, Northern blot analysis was performed using three probes derived from the shared region. A single 2.3 kb RNA transcript hybridized with all three probes (Figure 1B). Therefore, a cDNA lib ...
... clone encodes the ALV-B susceptibility factor (data not shown). To identify RNA transcripts encoding this factor, Northern blot analysis was performed using three probes derived from the shared region. A single 2.3 kb RNA transcript hybridized with all three probes (Figure 1B). Therefore, a cDNA lib ...
Catalog No
... Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation in rodents. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat ...
... Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation in rodents. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat ...
The RCK/p54 Prion-Related Domain and its Influence on the
... transduced with the lentivirus. To determine virus titer, cells were washed 2x with PBS and incubated with crystal violet dye (0.1% crystal violent, 20% ethanol), and the surviving cells were counted, and a viral titer was calculated. To concentrate the lentivirus, the transfection in 293FT cells wa ...
... transduced with the lentivirus. To determine virus titer, cells were washed 2x with PBS and incubated with crystal violet dye (0.1% crystal violent, 20% ethanol), and the surviving cells were counted, and a viral titer was calculated. To concentrate the lentivirus, the transfection in 293FT cells wa ...
THE MAMMALIAN TARGET OF RAPAMYCIN (MTOR) AS A
... Rapamycin has been a critical reagent in the elucidation of cell signaling pathways regulated by mTOR. The work of many investigators has established that mTOR regulates cell size and proliferation in part through control of protein synthesis of a subset of cell cycle regulatory proteins3,4. In yeas ...
... Rapamycin has been a critical reagent in the elucidation of cell signaling pathways regulated by mTOR. The work of many investigators has established that mTOR regulates cell size and proliferation in part through control of protein synthesis of a subset of cell cycle regulatory proteins3,4. In yeas ...
Embryonic stem cells form an organized, functional - AJP
... system in vitro. Although several molecular markers have been shown to delineate components of the cardiac conduction system in vivo, the functional characteristics of the cells expressing these markers remain unknown. The ability to accurately identify cells that function as cardiac pacemaking cell ...
... system in vitro. Although several molecular markers have been shown to delineate components of the cardiac conduction system in vivo, the functional characteristics of the cells expressing these markers remain unknown. The ability to accurately identify cells that function as cardiac pacemaking cell ...
The Cell - University of South Carolina
... In this example of a nerve cell stained with hematoxylin and eosin, you can see the extent to which you can resolve cell components in the using a light microscope. In this particular nerve cell, the nucleolus is large and the DNA in the nucleus is fully extended in a form that is called euchromatin ...
... In this example of a nerve cell stained with hematoxylin and eosin, you can see the extent to which you can resolve cell components in the using a light microscope. In this particular nerve cell, the nucleolus is large and the DNA in the nucleus is fully extended in a form that is called euchromatin ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.