Structural determinants for rCNT2 sorting to the plasma membrane
... to be a candidate to regulate the sorting of the transporter to the plasma membrane. To elucidate the possible sequences contained in this domain of rCNT2 responsible for this process, sequential deletions of the N-terminal tail were performed (Figure 1A). The expression ratios of the deleted constr ...
... to be a candidate to regulate the sorting of the transporter to the plasma membrane. To elucidate the possible sequences contained in this domain of rCNT2 responsible for this process, sequential deletions of the N-terminal tail were performed (Figure 1A). The expression ratios of the deleted constr ...
Hydrocortisone perturbs the cell proliferation pattern during feather
... incorporation of 5-bromo-2-deoxyuridine (BrdU) during successive stages of skin morphogenesis. In order to assess a putative relationship between cell proliferation and appendage building, we have considered the possibility of disturbing the normal developmental program in the skin. In agreement wit ...
... incorporation of 5-bromo-2-deoxyuridine (BrdU) during successive stages of skin morphogenesis. In order to assess a putative relationship between cell proliferation and appendage building, we have considered the possibility of disturbing the normal developmental program in the skin. In agreement wit ...
The Plant Cell - Gunawardena Lab
... cell death, the nucleus is the first target of degradation, and dying cells exhibit characteristic features such as chromatin condensation, nuclear shrinkage and fragmentation, and DNA laddering (Fukuda, 2000). Cell death is rapid, and the degradation of organelles may be incomplete. By contrast, ce ...
... cell death, the nucleus is the first target of degradation, and dying cells exhibit characteristic features such as chromatin condensation, nuclear shrinkage and fragmentation, and DNA laddering (Fukuda, 2000). Cell death is rapid, and the degradation of organelles may be incomplete. By contrast, ce ...
Glucocorticoid-Induced Plasma Membrane Depolarization during
... caspase inhibitor (z-VAD) blocked both cellular depolarization and repression of Naⴙ/Kⴙ-ATPase in both spontaneously dying and glucocorticoid-treated thymocytes; however, specific inhibitors of caspase 8, 9, and caspase 3 did not. Interestingly, glucocorticoid treatment simultaneously induced cell s ...
... caspase inhibitor (z-VAD) blocked both cellular depolarization and repression of Naⴙ/Kⴙ-ATPase in both spontaneously dying and glucocorticoid-treated thymocytes; however, specific inhibitors of caspase 8, 9, and caspase 3 did not. Interestingly, glucocorticoid treatment simultaneously induced cell s ...
ATPase in the plasma membrane of HeLa cells
... plays a critical role in controlling the quantity of the enzyme in the plasma membrane. There is an increasing number of reports on the structure and function of the auxiliary subunits of membrane proteins, including ion channels, receptors and ion-motive ATPases (Chow and Forte, 1995; Flucher et al ...
... plays a critical role in controlling the quantity of the enzyme in the plasma membrane. There is an increasing number of reports on the structure and function of the auxiliary subunits of membrane proteins, including ion channels, receptors and ion-motive ATPases (Chow and Forte, 1995; Flucher et al ...
Alight-inducible organelle targeting system for dynamically
... affinities and the reliance on drug washout (Haruki et al., 2008). In addition, pharmacological approaches lack generality; not all proteins can be easily designed for drug targeting. Compared to traditional genetic manipulations, the temporal and spatial precision of optogenetics allows perturbatio ...
... affinities and the reliance on drug washout (Haruki et al., 2008). In addition, pharmacological approaches lack generality; not all proteins can be easily designed for drug targeting. Compared to traditional genetic manipulations, the temporal and spatial precision of optogenetics allows perturbatio ...
Smart thermoresponsive coatings and surfaces for tissue
... trypsin and by chelating the Ca2+ and Mg2+ ions with ethylenediaminetetraacetic acid (EDTA). However, nonspecific proteases can damage crucial cell surface proteins, and this constitutes a major drawback of this cell harvesting method [6–10]. Moreover, from a tissue engineering perspective, the disr ...
... trypsin and by chelating the Ca2+ and Mg2+ ions with ethylenediaminetetraacetic acid (EDTA). However, nonspecific proteases can damage crucial cell surface proteins, and this constitutes a major drawback of this cell harvesting method [6–10]. Moreover, from a tissue engineering perspective, the disr ...
Lung function and immunopathological changes A.
... Parenteral or oral corticosteroids have been the mainstay of treatment for severe asthma for decades and more recently inhaled corticosteroids have been increasingly used with good effect in asthma of moderate severity [8]. However, the mode of action of corticosteroids in bronchial asthma is not fu ...
... Parenteral or oral corticosteroids have been the mainstay of treatment for severe asthma for decades and more recently inhaled corticosteroids have been increasingly used with good effect in asthma of moderate severity [8]. However, the mode of action of corticosteroids in bronchial asthma is not fu ...
Strategies and New Developments in the Generation of Patient-Specific Pluripotent Stem Cells Yamanaka, REVIEW (2007) Cell Stem Cell 1. July 2007 pp 39- 49.
... using adult somatic cells as donors and zygotes as recipients. Their new protocol involves arresting the recipient zygote in mitosis using drug treatment, removing its chromosomes and replacing them with donor-derived mitotic chromosomes. The mitotic arrest is key, because transfer to interphase zyg ...
... using adult somatic cells as donors and zygotes as recipients. Their new protocol involves arresting the recipient zygote in mitosis using drug treatment, removing its chromosomes and replacing them with donor-derived mitotic chromosomes. The mitotic arrest is key, because transfer to interphase zyg ...
On the mechanisms of nectar secretion: revisited
... Vassilyev — Mechanisms of nectar secretion photosynthate unloading and their further diffusion in the symplasm without entering the apoplasm. The situation in these systems appears to be similar to that occurring in nectaries devoid of their own phloem. Based on recent information regarding the cel ...
... Vassilyev — Mechanisms of nectar secretion photosynthate unloading and their further diffusion in the symplasm without entering the apoplasm. The situation in these systems appears to be similar to that occurring in nectaries devoid of their own phloem. Based on recent information regarding the cel ...
PDF
... green to allow for visual monitoring of the quantity of the injection. Injected embryos were allowed to continue to develop, then fixed 44 hours after the time when one half the clutch was at stage 6a (N teloblast formation). Individual micromeres were injected in each case with the exception of opq ...
... green to allow for visual monitoring of the quantity of the injection. Injected embryos were allowed to continue to develop, then fixed 44 hours after the time when one half the clutch was at stage 6a (N teloblast formation). Individual micromeres were injected in each case with the exception of opq ...
a comparative cytological and morphometric analysis of vacuolation
... In spite of a high cost of plant cell vacuolation, there are many benefits coming from increased area of cell surface per unit volume of cytoplasm of vacuolated cells. Compared with non-vacuolated, vacuolated cells more efficiently absorb ions and metabolites from even low external concentration. Th ...
... In spite of a high cost of plant cell vacuolation, there are many benefits coming from increased area of cell surface per unit volume of cytoplasm of vacuolated cells. Compared with non-vacuolated, vacuolated cells more efficiently absorb ions and metabolites from even low external concentration. Th ...
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats
... apoptosis can explain the reduction of insulin content in the whole pancreas and glucose-induced insulin secretion from the pancreas of rats treated with this thyroxine treatment schedule [5, 13]. Through application of a single dose of thyroxine (20 mg/kg b.wt. given i.p.) it was possible to synchr ...
... apoptosis can explain the reduction of insulin content in the whole pancreas and glucose-induced insulin secretion from the pancreas of rats treated with this thyroxine treatment schedule [5, 13]. Through application of a single dose of thyroxine (20 mg/kg b.wt. given i.p.) it was possible to synchr ...
Bio 103 Lecture - Origin and Evolution of Micr
... • How much oxygen was present in the early earth's atmosphere, how much is present today and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what was the temperature around the time life appeared, what caused the c ...
... • How much oxygen was present in the early earth's atmosphere, how much is present today and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what was the temperature around the time life appeared, what caused the c ...
AHCC and NK Cell Activity (Townsend Letter)
... Compliance was determined by how modified arabinoxylane group as a whole often patients purchased their supplements was significant, but the results varied from the author’s office using Quickbooks considerably between patients. Modified software and through patients’ verbal arabinoxylane tripled NK ...
... Compliance was determined by how modified arabinoxylane group as a whole often patients purchased their supplements was significant, but the results varied from the author’s office using Quickbooks considerably between patients. Modified software and through patients’ verbal arabinoxylane tripled NK ...
Contents - Hodder Education
... events that follow. The purple particles move from a region of high concentration (the dissolving crystal) to a region of low concentration (the surrounding water) until the concentration of purple particles (and water) is uniform throughout. ...
... events that follow. The purple particles move from a region of high concentration (the dissolving crystal) to a region of low concentration (the surrounding water) until the concentration of purple particles (and water) is uniform throughout. ...
Electron Microscopic Observations on the Excretion of Cell
... secretory process of the rabbit apocrine sweat glands (Kurosumi, 1962). This process has some similarity with the excretory activity of V. cholerae, as postulated in this study, but in detailed analysis many differences may again be detected, some of which could be due to the differences between the ...
... secretory process of the rabbit apocrine sweat glands (Kurosumi, 1962). This process has some similarity with the excretory activity of V. cholerae, as postulated in this study, but in detailed analysis many differences may again be detected, some of which could be due to the differences between the ...
Fate of Frog Virus 3 DNA Replicated in the Nucleus of Arginine
... supplemented with 10~o calf serum. To deprive the cells of arginine, this medium was replaced 16 h before infection by alpha medium devoid of arginine and supplemented with only 0.5~ dialysed calf serum. FV 3 was produced and purified as described previously (Aubertin et al., 1973, 1980). Infection ...
... supplemented with 10~o calf serum. To deprive the cells of arginine, this medium was replaced 16 h before infection by alpha medium devoid of arginine and supplemented with only 0.5~ dialysed calf serum. FV 3 was produced and purified as described previously (Aubertin et al., 1973, 1980). Infection ...
Day1 chick development
... The first day of chick development takes place inside the mother hen (in utero), during which the embryo progresses from fertilization to late blastula/early gastrula formation. The salient features of developmental anatomy in this period are conserved among the sauropsids (birds and reptiles). Many ...
... The first day of chick development takes place inside the mother hen (in utero), during which the embryo progresses from fertilization to late blastula/early gastrula formation. The salient features of developmental anatomy in this period are conserved among the sauropsids (birds and reptiles). Many ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.