Early Development of the Japanese Spiny Oyster (Saccostrea
... Digoxigenin-labeled RNA probes were synthesized in vitro from cDNA clones by using SP6 RNA polymerase (Invitrogen) and DIG RNA labeling mix (Roche). The embryos were fixed in 4% paraformaldehyde, 0.1 M MOPS (pH 7.5), 2 mM EGTA, and 0.5 M NaCl and stored in 80% ethanol at –20°C. In-situ hybridization ...
... Digoxigenin-labeled RNA probes were synthesized in vitro from cDNA clones by using SP6 RNA polymerase (Invitrogen) and DIG RNA labeling mix (Roche). The embryos were fixed in 4% paraformaldehyde, 0.1 M MOPS (pH 7.5), 2 mM EGTA, and 0.5 M NaCl and stored in 80% ethanol at –20°C. In-situ hybridization ...
The immunological synapse: a focal point for endocytosis and
... The precise site of signaling events leading to immune synapse formation has been controversial (for review see Dustin, 2009). Early signaling events have been shown to occur in peripheral microclusters in the pSMAC that coalesce into the cSMAC in both T and B cell synapses (Campi et al., 2005; Yoko ...
... The precise site of signaling events leading to immune synapse formation has been controversial (for review see Dustin, 2009). Early signaling events have been shown to occur in peripheral microclusters in the pSMAC that coalesce into the cSMAC in both T and B cell synapses (Campi et al., 2005; Yoko ...
A balanced pyrimidine pool is required for optimal Chk1 activation to
... CDA deficiency leads to an intracellular accumulation of dCTP (Chabosseau et al., 2011; Gemble et al., 2015). The addition of dC to the culture medium of CDA-proficient cells reproduces the increase in dCTP levels observed in CDA-deficient cells, mimicking CDA deficiency (Chabosseau et al., 2011; Ge ...
... CDA deficiency leads to an intracellular accumulation of dCTP (Chabosseau et al., 2011; Gemble et al., 2015). The addition of dC to the culture medium of CDA-proficient cells reproduces the increase in dCTP levels observed in CDA-deficient cells, mimicking CDA deficiency (Chabosseau et al., 2011; Ge ...
Histone H3 Phosphorylation Is Coupled to Poly-(ADP
... which they interact with cellular constituents, as well as the consequences of such interactions, are important fundamental goals of biomedical research. Renal proximal tubule epithelial cells are particularly sensitive to oxidant-mediated injury. Using a well-established in vitro model of renal pro ...
... which they interact with cellular constituents, as well as the consequences of such interactions, are important fundamental goals of biomedical research. Renal proximal tubule epithelial cells are particularly sensitive to oxidant-mediated injury. Using a well-established in vitro model of renal pro ...
Artificial Salivary Glands
... cell types. Formation, maintenance, and repair of the tissue in which they reside are the primary roles of the adult stem cell. Although adult stem cells may not be as powerful or diverse as embryonic stem cells, they offer many advantages for the development of cellular therapeutics including lack ...
... cell types. Formation, maintenance, and repair of the tissue in which they reside are the primary roles of the adult stem cell. Although adult stem cells may not be as powerful or diverse as embryonic stem cells, they offer many advantages for the development of cellular therapeutics including lack ...
Second Harmonic Imaging of Plant Polysaccharides
... of symmetry to the molecule, though the chirality of the glucose means that the symmetry is not complete. (Only the dextro form (D-glucose) is found in plants). Starch, on the other hand, is α 1:4 linked8, so each glucose molecule in the chain is in the same orientation, making the chain much less s ...
... of symmetry to the molecule, though the chirality of the glucose means that the symmetry is not complete. (Only the dextro form (D-glucose) is found in plants). Starch, on the other hand, is α 1:4 linked8, so each glucose molecule in the chain is in the same orientation, making the chain much less s ...
Networks Inferred from Biochemical Data Reveal Profound
... the third most common cause of cancer death in humans (44). Thus, better understanding of changes in signaling networks that accompany the development of HCC should impact human health. Whereas transcriptional data can be collected on a genome-wide scale, this is not possible for data on protein lev ...
... the third most common cause of cancer death in humans (44). Thus, better understanding of changes in signaling networks that accompany the development of HCC should impact human health. Whereas transcriptional data can be collected on a genome-wide scale, this is not possible for data on protein lev ...
Question paper - Unit F222/01 - Growth, development and
... (h) In the case study, you were given information about some scientific studies into the effects of soy food on different health conditions. Two of these studies are listed below. ...
... (h) In the case study, you were given information about some scientific studies into the effects of soy food on different health conditions. Two of these studies are listed below. ...
Large Scale Dimethyl Histone H3 Lysine-9 Blocks
... regions are evolutionarily conserved, G9a-dependent, and linked to changes in gene expression in a tissue-specific manner, and they are substantially lost in human cancer cell lines. These results have important implications for the epigenetic regulation of gene expression. While organization of the ...
... regions are evolutionarily conserved, G9a-dependent, and linked to changes in gene expression in a tissue-specific manner, and they are substantially lost in human cancer cell lines. These results have important implications for the epigenetic regulation of gene expression. While organization of the ...
Lethality of radioisotopes in early mouse embryos
... label. This was achieved by the addition of non-radioactive methionine to the culture medium. Results of such an experiment are shown in Table 1. It is clear that survival can indeed be increased by lowering the specific activity to 002 Ci/mmol. Furthermore, it could be shown, in separate experiment ...
... label. This was achieved by the addition of non-radioactive methionine to the culture medium. Results of such an experiment are shown in Table 1. It is clear that survival can indeed be increased by lowering the specific activity to 002 Ci/mmol. Furthermore, it could be shown, in separate experiment ...
Origin of Metazoa E
... with the planula larva of coelenterates. According to this view, the radial symmetry of coelenterates is primitive and derived directly from this hypothetical planuloid ancestor. ...
... with the planula larva of coelenterates. According to this view, the radial symmetry of coelenterates is primitive and derived directly from this hypothetical planuloid ancestor. ...
PDF
... cells. This result was to be expected, since some hemiganglia on the ablated side are of nearly normal size and others are smaller or completely missing. However, an unexpected result was found also, namely, that in the left hemiganglia of the abnormal zone, there were some patches of cells that did ...
... cells. This result was to be expected, since some hemiganglia on the ablated side are of nearly normal size and others are smaller or completely missing. However, an unexpected result was found also, namely, that in the left hemiganglia of the abnormal zone, there were some patches of cells that did ...
A level Biology Summer Homework - North Bristol Post
... This work is set to help you make the transition from Year 11 to your Post-16 studies. It is very important that you complete it to a high standard as it will help you start to build to the skills you will need to do well at the North Bristol Post-16 Centre. 1. Complete the questions attached and br ...
... This work is set to help you make the transition from Year 11 to your Post-16 studies. It is very important that you complete it to a high standard as it will help you start to build to the skills you will need to do well at the North Bristol Post-16 Centre. 1. Complete the questions attached and br ...
Antibacterial dugs and their interference with the
... available if patients that are treated with the current doses of the antibiotics. Antimitochondrial levels are reached, however, during such treatments (I2 13). In table III some data on the tour most commonly used tetracyclines are brought together. The experiments in which the inhibitory effects o ...
... available if patients that are treated with the current doses of the antibiotics. Antimitochondrial levels are reached, however, during such treatments (I2 13). In table III some data on the tour most commonly used tetracyclines are brought together. The experiments in which the inhibitory effects o ...
FM Dyes Label Sterol-Rich Plasma Membrane
... the distribution of the FM patches and that of mitochondria as long as the observation time did not exceed 2 h (Fig. 1L). In pollen tubes, FM dyes are rapidly transferred to secretory vesicles which accumulate at the growing tip (see, for example, Hörmanseder et al. 2005). In Chara, the accumulatio ...
... the distribution of the FM patches and that of mitochondria as long as the observation time did not exceed 2 h (Fig. 1L). In pollen tubes, FM dyes are rapidly transferred to secretory vesicles which accumulate at the growing tip (see, for example, Hörmanseder et al. 2005). In Chara, the accumulatio ...
The Care and Feeding of the Microscope Lab 5
... Microscopists improve contrast by coloring specimens with stains (dyes) that bind to cellular structures and absorb light to provide contrast. ...
... Microscopists improve contrast by coloring specimens with stains (dyes) that bind to cellular structures and absorb light to provide contrast. ...
Biogenesis of MalF and the MalFGK Maltose Transport Escherichia coli 2
... Refs. 12–15). It has been suggested that YidC mediates the transfer of TMs from the Sec translocon into the lipid bilayer (1). Indeed, YidC could be co-purified with the Sec translocon, suggesting a physical connection (14). A recent in vitro study using lactose permease (LacY) reveals a novel funct ...
... Refs. 12–15). It has been suggested that YidC mediates the transfer of TMs from the Sec translocon into the lipid bilayer (1). Indeed, YidC could be co-purified with the Sec translocon, suggesting a physical connection (14). A recent in vitro study using lactose permease (LacY) reveals a novel funct ...
Syrosingopine sensitizes cancer cells to killing by metformin
... widely prescribed drug for type 2 diabetes. It acts as a mild mitochondrial inhibitor, and its impact on key metabolic organs, such as the liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug t ...
... widely prescribed drug for type 2 diabetes. It acts as a mild mitochondrial inhibitor, and its impact on key metabolic organs, such as the liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug t ...
Cellular Polarity in Prokaryotic Organisms
... affinity for cell poles. B. subtilis DivIVA is found at the cell poles and this localization is required for both the polar localization of MinC, a protein necessary for proper mid-cell division (Marston et al. 1998), as well as that of RacA, a protein necessary for polar attachment of chromosomes d ...
... affinity for cell poles. B. subtilis DivIVA is found at the cell poles and this localization is required for both the polar localization of MinC, a protein necessary for proper mid-cell division (Marston et al. 1998), as well as that of RacA, a protein necessary for polar attachment of chromosomes d ...
Variations of bacterial-specific activity with cell size and nucleic acid
... ABSTRACT: In most aquatic bacterial communities, it is possible to discriminate bacterial cells with a high nucleic acid content (HNA) from those with a low nucleic acid content (LNA) by flow cytometry. The distribution of leucine incorporation rate per cell (specific activity) within the fraction o ...
... ABSTRACT: In most aquatic bacterial communities, it is possible to discriminate bacterial cells with a high nucleic acid content (HNA) from those with a low nucleic acid content (LNA) by flow cytometry. The distribution of leucine incorporation rate per cell (specific activity) within the fraction o ...
The Plant Cell - Gunawardena Lab
... cell death, the nucleus is the first target of degradation, and dying cells exhibit characteristic features such as chromatin condensation, nuclear shrinkage and fragmentation, and DNA laddering (Fukuda, 2000). Cell death is rapid, and the degradation of organelles may be incomplete. By contrast, ce ...
... cell death, the nucleus is the first target of degradation, and dying cells exhibit characteristic features such as chromatin condensation, nuclear shrinkage and fragmentation, and DNA laddering (Fukuda, 2000). Cell death is rapid, and the degradation of organelles may be incomplete. By contrast, ce ...
Formation and maintenance of the shoot apical meristem
... Fig. 2. Expression patterns of genes directing the establishment and maintenance of the shoot apical meristem (SAM) form gradually during embryogenesis. (a–f) Expression patterns of six genes: the upper panel depicts the expression in a frontal section9 of a late globular-transition stage of embryog ...
... Fig. 2. Expression patterns of genes directing the establishment and maintenance of the shoot apical meristem (SAM) form gradually during embryogenesis. (a–f) Expression patterns of six genes: the upper panel depicts the expression in a frontal section9 of a late globular-transition stage of embryog ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.