Transcriptional insights into the CD8+ T cell response to infection
... visualize changes in gene expression over the course of the CD8 + T cell response to infection with Lm-OVA, we selected 7,195 genes that had a difference in expression of at least twofold in any two samples in the data set from the total of 25,194 genes examined (Fig. 1b and Supplementary Table 1). ...
... visualize changes in gene expression over the course of the CD8 + T cell response to infection with Lm-OVA, we selected 7,195 genes that had a difference in expression of at least twofold in any two samples in the data set from the total of 25,194 genes examined (Fig. 1b and Supplementary Table 1). ...
cell cycle
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor ...
... They may make their own growth factor They may convey a growth factor’s signal without the presence of the growth factor ...
Great Expectations for PIP: Phosphoinositides as Regulators of
... membranes, such as the localization of FYVE domain containing trafficking proteins to endosomes. However, it is important to emphasize that PIP binding can also play a direct role in regulating protein-protein interactions and catalytic activities (see for example Corgan et al., 2004; Takei et al., ...
... membranes, such as the localization of FYVE domain containing trafficking proteins to endosomes. However, it is important to emphasize that PIP binding can also play a direct role in regulating protein-protein interactions and catalytic activities (see for example Corgan et al., 2004; Takei et al., ...
ARVO 2017 Annual Meeting Abstracts 306 Lens Physiology and
... cells remains largely unknown. Methods: Lens primary cell cultures were prepared using embryonic day-11 fertilized chicken eggs and were cultured up to 15 days with the gradual formation of lentoid body structures, an indicator of lens cell differentiation. High titer recombinant retroviruses contai ...
... cells remains largely unknown. Methods: Lens primary cell cultures were prepared using embryonic day-11 fertilized chicken eggs and were cultured up to 15 days with the gradual formation of lentoid body structures, an indicator of lens cell differentiation. High titer recombinant retroviruses contai ...
The Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and
... were grown for 16 h at 37 °C before harvesting. For reference, other samples were labeled for 2 h with [35S]methionine in the presence of cycloheximide and then immediately harvested. Analysis of Mitochondrial Ribosomes and Polysomes—For the analysis of mitochondrial polysomes, cells were labeled wi ...
... were grown for 16 h at 37 °C before harvesting. For reference, other samples were labeled for 2 h with [35S]methionine in the presence of cycloheximide and then immediately harvested. Analysis of Mitochondrial Ribosomes and Polysomes—For the analysis of mitochondrial polysomes, cells were labeled wi ...
Staphylococcus aureus-induced G2/M phase transition delay
... and Bordetella pertussis adenylate cyclase toxin increase the proportion of cells in the G1/G0 phase. [14]. Exposure of eukaryotic cells to the cytolethal distending toxin of E. coli results in arrest in both the G1 and G2 phases [12,15]. Pathogen-induced cell cycle alteration may be linked to the i ...
... and Bordetella pertussis adenylate cyclase toxin increase the proportion of cells in the G1/G0 phase. [14]. Exposure of eukaryotic cells to the cytolethal distending toxin of E. coli results in arrest in both the G1 and G2 phases [12,15]. Pathogen-induced cell cycle alteration may be linked to the i ...
β Glucocorticoids on IL-1 Counteract the Inhibitory Effect of Migration
... phospholipase A2 activity; down-regulate the synthesis and secretion of IL-1, IL-6, and TNF-␣ from activated monocytes and macrophages; and inhibit cytokine-induced transcription factors, such as NF-B and AP1 (32, 33). The crucial role of glucocorticoids has been demonstrated in a number of studies ...
... phospholipase A2 activity; down-regulate the synthesis and secretion of IL-1, IL-6, and TNF-␣ from activated monocytes and macrophages; and inhibit cytokine-induced transcription factors, such as NF-B and AP1 (32, 33). The crucial role of glucocorticoids has been demonstrated in a number of studies ...
Should reflectance confocal microscopy be the gold standard for
... morpheaform [5] . It is a slow growing tumor with a very low metastatic capability. Owing to its frequency (e.g., 1383 cases per 100,000 people in Australia in 2008) and relatively low mortality, BCC remains one of the best characterized types of skin cancer [6] . RCM has been extensively performed ...
... morpheaform [5] . It is a slow growing tumor with a very low metastatic capability. Owing to its frequency (e.g., 1383 cases per 100,000 people in Australia in 2008) and relatively low mortality, BCC remains one of the best characterized types of skin cancer [6] . RCM has been extensively performed ...
Clarkson University
... nitrocellulose paper was then placed face down on C. neoformans plated agar plates. It was hoped that the conditioned medium would inhibit the growth of the yeast only along a certain line of the gel. If the concentration of the conditioned BV2 supernatant was high enough then the yeast inhibition ...
... nitrocellulose paper was then placed face down on C. neoformans plated agar plates. It was hoped that the conditioned medium would inhibit the growth of the yeast only along a certain line of the gel. If the concentration of the conditioned BV2 supernatant was high enough then the yeast inhibition ...
performance of nonlinear visual units in ocular hypertension
... obtained from both methods showed that the analysis. The first two principal components measured variances were heterogeneous and were indeed primarily correlated and composed that a log transform was indicated for sub- of either the five modulated gratings or the sequent analysis. When the log tran ...
... obtained from both methods showed that the analysis. The first two principal components measured variances were heterogeneous and were indeed primarily correlated and composed that a log transform was indicated for sub- of either the five modulated gratings or the sequent analysis. When the log tran ...
Transport of intravenously-injected ferritin across the guinea
... off under the dissecting microscope; some was processed for light microscopy and stained with haematoxylin and eosin, Perls's stain, and neutral red. Sections from the liver, kidney, and spleen were stained similarly for light microscopy to confirm the presence of iron in the tissues of the experime ...
... off under the dissecting microscope; some was processed for light microscopy and stained with haematoxylin and eosin, Perls's stain, and neutral red. Sections from the liver, kidney, and spleen were stained similarly for light microscopy to confirm the presence of iron in the tissues of the experime ...
Stem cells in cancer: instigators and propagators?
... In the mouse small intestine there is both a slowly cycling stem cell population found ~4-5 cell positions above the base of the crypt (Sangiorgi and Capecchi, 2008), and a rapidly cycling stem cell population composed of slender-shaped cells [so-called crypt base columnar cells (CBCCs)] that are sa ...
... In the mouse small intestine there is both a slowly cycling stem cell population found ~4-5 cell positions above the base of the crypt (Sangiorgi and Capecchi, 2008), and a rapidly cycling stem cell population composed of slender-shaped cells [so-called crypt base columnar cells (CBCCs)] that are sa ...
CELL DIVISION AND DNA SYNTHESIS IN TETRAHYMENA
... divided with 75-minute delay, i.e., identical to the previous three groups, and those that divided without any delay. In all cases, the effects of transfer were evaluated against the generation time of cells subjected to the same type of medium shift but without amino acid deprivation. ...
... divided with 75-minute delay, i.e., identical to the previous three groups, and those that divided without any delay. In all cases, the effects of transfer were evaluated against the generation time of cells subjected to the same type of medium shift but without amino acid deprivation. ...
Cross-Immunoreactivity between Bacterial Aquaporin
... that regulate water flow in cells (7). They are expressed by various mammalian cell types and bacteria, and they display similar threedimensional loop/helix structure across the plasma membrane (8). Mutations in certain aquaporin genes have been associated with human hereditary diseases (9). Aqp4 ha ...
... that regulate water flow in cells (7). They are expressed by various mammalian cell types and bacteria, and they display similar threedimensional loop/helix structure across the plasma membrane (8). Mutations in certain aquaporin genes have been associated with human hereditary diseases (9). Aqp4 ha ...
A kalpain enzimrendszer idegrendszeri plaszticitást befolyásoló
... lidocaine displayed substantially higher potencies when applied after the maximal depolarizing action of VER had already developed (post-treatment protocol), as compared with the situation when their application preceded that of the toxin (pre-treatment protocol). Results obtained in the post-treatm ...
... lidocaine displayed substantially higher potencies when applied after the maximal depolarizing action of VER had already developed (post-treatment protocol), as compared with the situation when their application preceded that of the toxin (pre-treatment protocol). Results obtained in the post-treatm ...
Chapter 1 Histology and its methods of study
... Live cells and tissues can be maintained and studied outside the body. cell culture has been very useful in isolating the effects of single molecules on specific types of cells. It also allows the direct observation of the behavior of living cells under the phase contrast microscope. Many experiment ...
... Live cells and tissues can be maintained and studied outside the body. cell culture has been very useful in isolating the effects of single molecules on specific types of cells. It also allows the direct observation of the behavior of living cells under the phase contrast microscope. Many experiment ...
Cellular Force Microscopy for in Vivo
... forces in the nanonewton range (Fig. 1). Hence, the cell wall was essentially compressed over a submicrometric volume, measuring the elastic modulus in the direction normal to its surface, as opposed to wall elasticity in the surface plane (Milani et al., 2011). Given the composite structure of the ...
... forces in the nanonewton range (Fig. 1). Hence, the cell wall was essentially compressed over a submicrometric volume, measuring the elastic modulus in the direction normal to its surface, as opposed to wall elasticity in the surface plane (Milani et al., 2011). Given the composite structure of the ...
Temporal Precision of Spike Trains in Extrastriate
... as the standard deviation (SD), or jitter, of the beginning or ending time of a period of elevated firing, e.g. the periods indicated by the thick lines below the PSTHs at 1750 msec in Fig. 1 and near 1600 msec in Fig. 2. A. The distribution across trials of the occurrence time for the first action ...
... as the standard deviation (SD), or jitter, of the beginning or ending time of a period of elevated firing, e.g. the periods indicated by the thick lines below the PSTHs at 1750 msec in Fig. 1 and near 1600 msec in Fig. 2. A. The distribution across trials of the occurrence time for the first action ...
Steps
... Involves exposure of patient’s skin to potential allergens and observing its reaction. Principle : Re-exposure to the culprit allergen (drug) will elicit similar clinical reaction pattern in previously sensitized individuals. Uses : Allergic contact dermatitis, also useful in drug reactions. Metho ...
... Involves exposure of patient’s skin to potential allergens and observing its reaction. Principle : Re-exposure to the culprit allergen (drug) will elicit similar clinical reaction pattern in previously sensitized individuals. Uses : Allergic contact dermatitis, also useful in drug reactions. Metho ...
Break of symmetry in regenerating tobacco protoplasts is
... cases of symmetrical, freely accessible cells, which undergo formative divisions, are rare in higher plants. As an alternative to studying polarity induction de novo, polarity can be artificially eliminated by digesting the cell wall with cellulases. This tabula rasa approach yields protoplasts, whic ...
... cases of symmetrical, freely accessible cells, which undergo formative divisions, are rare in higher plants. As an alternative to studying polarity induction de novo, polarity can be artificially eliminated by digesting the cell wall with cellulases. This tabula rasa approach yields protoplasts, whic ...
Bacteria
... When you think of bacteria, you probably create images of all the bad things they can do to you. Most of our hand soap comes with anti-bacterial ingredients that help kill off harmful bacteria. Keep in mind that most bacteria are not bad. The human body carries more than 100 trillion bacteria (about ...
... When you think of bacteria, you probably create images of all the bad things they can do to you. Most of our hand soap comes with anti-bacterial ingredients that help kill off harmful bacteria. Keep in mind that most bacteria are not bad. The human body carries more than 100 trillion bacteria (about ...
University of Birmingham Leptomycin B induces apoptosis in cells
... variation in this expression between the lines (Fig. 2b). Treatment with 2 nM LMB (38 h) caused a marked increase in act-casp-3 expression in all of the W12 derivatives (Fig. 2b). The most marked effect was seen in the E50 cells, with the remaining derivatives all showing lesser responses, particula ...
... variation in this expression between the lines (Fig. 2b). Treatment with 2 nM LMB (38 h) caused a marked increase in act-casp-3 expression in all of the W12 derivatives (Fig. 2b). The most marked effect was seen in the E50 cells, with the remaining derivatives all showing lesser responses, particula ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.