THE MAMMALIAN CELL VIRUS RELATIONSHIP (From the
... at 4°C. (used within a week of opening); and (c) ethylenediamine sodium tetraazetate (versene) for addition to the primary virus suspension in final concentration of 5 X 10-a M to prevent inactivation of nucleic acid by metal ions (11). Three to six ml. quantities of virus were mixed with equal amou ...
... at 4°C. (used within a week of opening); and (c) ethylenediamine sodium tetraazetate (versene) for addition to the primary virus suspension in final concentration of 5 X 10-a M to prevent inactivation of nucleic acid by metal ions (11). Three to six ml. quantities of virus were mixed with equal amou ...
The Australian Stem Cell Handbook
... MSCs have a possible role in regeneration of bone, cartilage and fat and have anti-inflammatory and immunosuppressive properties. They are currently being used by researchers to: • investigate possible regenerative therapies including orthopaedic and cardiovascular conditions • suppress gra ...
... MSCs have a possible role in regeneration of bone, cartilage and fat and have anti-inflammatory and immunosuppressive properties. They are currently being used by researchers to: • investigate possible regenerative therapies including orthopaedic and cardiovascular conditions • suppress gra ...
1 a dictyostelium mutant with reduced lysozyme levels compensates
... the level of expression of the lysozyme P isoform to compensate for their deficiency, they remain much more sensitive to infections of the lung (14). Lysozymes are not only found in animals but are also present in numerous phylogenetically diverse organisms such as plants, fungi, bacteria, and bacte ...
... the level of expression of the lysozyme P isoform to compensate for their deficiency, they remain much more sensitive to infections of the lung (14). Lysozymes are not only found in animals but are also present in numerous phylogenetically diverse organisms such as plants, fungi, bacteria, and bacte ...
Two-photon laser scanning microscopy imaging of intact spinal cord and
... 2007). Induction of the microglial injury response is a sensitive measure of such damage (Davalos et al., 2005; Kim and Dustin, 2006). The underlying tissue is also potentially more vulnerable to additional inflammatory artifacts such as acute neutrophil recruitment (Kim and Dustin, 2006). These shor ...
... 2007). Induction of the microglial injury response is a sensitive measure of such damage (Davalos et al., 2005; Kim and Dustin, 2006). The underlying tissue is also potentially more vulnerable to additional inflammatory artifacts such as acute neutrophil recruitment (Kim and Dustin, 2006). These shor ...
PIERGIORGIO PETTAZZONI
... 4-Hydoxynonenal (HNE), one of the most important aldheydic products generated from the lipidic peroxidation of ω-6 polyunsaturated fatty acid, has been suggested to play a critical role in the pathogenesis of various diseases, such as atherosclerosis, diabetes, Alzheimer and Parkinson diseases. It h ...
... 4-Hydoxynonenal (HNE), one of the most important aldheydic products generated from the lipidic peroxidation of ω-6 polyunsaturated fatty acid, has been suggested to play a critical role in the pathogenesis of various diseases, such as atherosclerosis, diabetes, Alzheimer and Parkinson diseases. It h ...
Slide 1
... become specialized in structure and function The physical processes that give an organism its shape constitute morphogenesis ...
... become specialized in structure and function The physical processes that give an organism its shape constitute morphogenesis ...
Nance et al gastrulation paper - The Hardin Lab
... ingression is associated with changes in the shape of the ingressing cells and a redistribution of non-muscle myosin. Finally, we present evidence that cell fate, rather than cell position, is the predominant factor regulating ingression, although steric interactions can play an important role. ...
... ingression is associated with changes in the shape of the ingressing cells and a redistribution of non-muscle myosin. Finally, we present evidence that cell fate, rather than cell position, is the predominant factor regulating ingression, although steric interactions can play an important role. ...
Antiproliferative and apoptotic effects of O
... on proliferation and apoptosis in cultures of the human hepatoblastoma HepG2 and hepatocarcinoma HBG cell lines. Our results show that TRX decreased DNA synthesis in a time- and dose-dependent manner and with a higher efficiency than DFO. Mitotic index was also strongly decreased by TRX and, unexpec ...
... on proliferation and apoptosis in cultures of the human hepatoblastoma HepG2 and hepatocarcinoma HBG cell lines. Our results show that TRX decreased DNA synthesis in a time- and dose-dependent manner and with a higher efficiency than DFO. Mitotic index was also strongly decreased by TRX and, unexpec ...
improving methodological strategies for satellite cells counting in
... To enable comparison of data obtained from specimens frozen at different sarcomere length, both stereological parameters estimated, i.e., number of satellite cells per fibre length NL(sc, fib) and number of nuclei per fibre length NL(nucl, fib), were corrected to a reference sarcomere length, i.e. d ...
... To enable comparison of data obtained from specimens frozen at different sarcomere length, both stereological parameters estimated, i.e., number of satellite cells per fibre length NL(sc, fib) and number of nuclei per fibre length NL(nucl, fib), were corrected to a reference sarcomere length, i.e. d ...
The Ndc80p Complex from Saccharomyces cerevisiae Contains
... to select transformants, ⵑ80% of those examined, which did not contain deletions introduced by the oligos. For observations of green fluorescence protein (GFP) in live S. pombe, cells were grown in EMM2 (Moreno et al., 1991) and observed on 2% agarose/EMM2 pads with a video camera (RTEA/CCD-1800-Y; ...
... to select transformants, ⵑ80% of those examined, which did not contain deletions introduced by the oligos. For observations of green fluorescence protein (GFP) in live S. pombe, cells were grown in EMM2 (Moreno et al., 1991) and observed on 2% agarose/EMM2 pads with a video camera (RTEA/CCD-1800-Y; ...
Characterization and Expression of Monosaccharide Transporters
... Laboratory of Biophysics, School of Medicine, Teikyo University, Hachioji, Tokyo, 192-0395 Japan ...
... Laboratory of Biophysics, School of Medicine, Teikyo University, Hachioji, Tokyo, 192-0395 Japan ...
Streptococcal Cell Wall-Induced Arthritis
... Intra-articular injection of streptococcal cell wall Ag followed by i.v. challenge (“reactivation”) results in a destructive lymphocytedependent monoarticular arthritis. To further define the role of immune mechanisms in the model, Abs to Th1 and Th2-related cytokines were evaluated. Treatment of ra ...
... Intra-articular injection of streptococcal cell wall Ag followed by i.v. challenge (“reactivation”) results in a destructive lymphocytedependent monoarticular arthritis. To further define the role of immune mechanisms in the model, Abs to Th1 and Th2-related cytokines were evaluated. Treatment of ra ...
Dendritic cells express tight junction proteins and penetrate gut
... To study the interaction of DCs with epithelial cells, we established a transwell coculture system in vitro. We cultured a monolayer of the differentiated human enterocyte cell line Caco-2 on the lower face of 6.5mm Corning filters until a transepithelial electric resistance (TER) of ∼330 Ω/cm2 was ...
... To study the interaction of DCs with epithelial cells, we established a transwell coculture system in vitro. We cultured a monolayer of the differentiated human enterocyte cell line Caco-2 on the lower face of 6.5mm Corning filters until a transepithelial electric resistance (TER) of ∼330 Ω/cm2 was ...
Attachment to and biofilm formation on abiotic
... surfaces in hospital environments and colonizes different medical devices. In this study the analysis of the processes involved in surface attachment and biofilm formation by the prototype strain 19606 was initiated. This strain attaches to and forms biofilm structures on plastic and glass surfaces, ...
... surfaces in hospital environments and colonizes different medical devices. In this study the analysis of the processes involved in surface attachment and biofilm formation by the prototype strain 19606 was initiated. This strain attaches to and forms biofilm structures on plastic and glass surfaces, ...
Paul M. Nurse - Nobel Lecture
... which blocked in G1 phase prior to commitment to the cell cycle. The approach followed was that of Lee Hartwell, who had reasoned that budding yeast mutants blocked early in the cell cycle prior to commitment would still be able to conjugate if challenged to do so. Cdc2ts mutants were used as negat ...
... which blocked in G1 phase prior to commitment to the cell cycle. The approach followed was that of Lee Hartwell, who had reasoned that budding yeast mutants blocked early in the cell cycle prior to commitment would still be able to conjugate if challenged to do so. Cdc2ts mutants were used as negat ...
FORMULATION AND IN VITRO EVALUATION OF NIOSOMAL POVIDONE –IODINE CARRIERS
... currently available to aid in the sustained slow release of PVP-I from an absorbent material. Hence, in the current investigation niosome preparations encapsulating PVP-I were developed and studied. Efficacy of the chemical antiseptic was determined in the absence of organic matter. The MIC of PVP-I ...
... currently available to aid in the sustained slow release of PVP-I from an absorbent material. Hence, in the current investigation niosome preparations encapsulating PVP-I were developed and studied. Efficacy of the chemical antiseptic was determined in the absence of organic matter. The MIC of PVP-I ...
Brain tumour research (Dept of Neurosurgery RPH and BTNW)
... In our experiments, we have been successful in demonstrating the growth suppressive effects of Mifepristone on glioma cell lines. We were however not able to demonstrate growth stimulation by either Progesterone or Dexamethasone to any significant degree. Also, we found that effect of Mifepristone w ...
... In our experiments, we have been successful in demonstrating the growth suppressive effects of Mifepristone on glioma cell lines. We were however not able to demonstrate growth stimulation by either Progesterone or Dexamethasone to any significant degree. Also, we found that effect of Mifepristone w ...
Epithelial repair is a two-stage process driven first by dying cells and
... releasing the apical parts into the lumen (Bird et al., 2010). In cultured epithelial monolayers, dying cells can be extruded from the epithelium either apically (Rosenblatt et al., 2001) or basally (Slattum et al., 2009), and the latter has been suggested to be a possible cause of cancer progressio ...
... releasing the apical parts into the lumen (Bird et al., 2010). In cultured epithelial monolayers, dying cells can be extruded from the epithelium either apically (Rosenblatt et al., 2001) or basally (Slattum et al., 2009), and the latter has been suggested to be a possible cause of cancer progressio ...
Essential embryonic roles of the CKI-1 cyclin
... cycle arrest, morphogenesis and differentiation, and suppression of programmed cell death. The vertebrate Cip/Kip CKIs have been shown to directly inhibit the activity of cyclin/cyclin-dependent kinase (cdk) complexes that control G1/S progression, leading to G1 arrest (Sherr and Roberts, 1995). The ...
... cycle arrest, morphogenesis and differentiation, and suppression of programmed cell death. The vertebrate Cip/Kip CKIs have been shown to directly inhibit the activity of cyclin/cyclin-dependent kinase (cdk) complexes that control G1/S progression, leading to G1 arrest (Sherr and Roberts, 1995). The ...
signaling events regulating removal of apoptotic cells
... to have an overlapping tissue distribution in the mouse (Gumienny et al., 2001). However, in situ studies of ELMO1 and ELMO2 in the mouse brain suggest these proteins play cell-type-specific roles (Katoh et al., 2006a). The regulation of ELMO-Dock180 activation appears surprisingly complex. Prelimin ...
... to have an overlapping tissue distribution in the mouse (Gumienny et al., 2001). However, in situ studies of ELMO1 and ELMO2 in the mouse brain suggest these proteins play cell-type-specific roles (Katoh et al., 2006a). The regulation of ELMO-Dock180 activation appears surprisingly complex. Prelimin ...
Lysosomal Heterogeneity in Exocrine Acinar Cells
... marker enzyme for lysosomes (3). In exocrine secretory cells (2,4), AcPase activity is localized in GERL, immature secretory granules, and some, but not all, lysosomes (Figure 1). The lysosomes that possess AcPase activity are generally located adjacent to the Golgi apparatus, frequently, on the cis ...
... marker enzyme for lysosomes (3). In exocrine secretory cells (2,4), AcPase activity is localized in GERL, immature secretory granules, and some, but not all, lysosomes (Figure 1). The lysosomes that possess AcPase activity are generally located adjacent to the Golgi apparatus, frequently, on the cis ...
Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by
... Skb5 deletion cells would be expected to exhibit phenotypes similar to those associated with Pmp1 deletion. As shown in Fig. 1D, Skb5 deletion induced hypersensitivity to 0.6 M MgCl2 as did Pmp1 deletion. However, cells with an Skb5 deletion (Δskb5) did not exhibit sensitivity to FK506, whereas the ...
... Skb5 deletion cells would be expected to exhibit phenotypes similar to those associated with Pmp1 deletion. As shown in Fig. 1D, Skb5 deletion induced hypersensitivity to 0.6 M MgCl2 as did Pmp1 deletion. However, cells with an Skb5 deletion (Δskb5) did not exhibit sensitivity to FK506, whereas the ...
How to present a scientific paper
... Again, use of Hsf1 -/- cells is a way to confirm siRNA results ...
... Again, use of Hsf1 -/- cells is a way to confirm siRNA results ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.