Full Text - The International Journal of Developmental Biology
... splanchnopleure during early extraembryonic mesoderm patternand hemangioblasts. Wolff (1764 Theorie von der generation) is ing. Among these three lineages, progenitors for the smooth generally credited for the earliest description of the blood islands. muscle cells, contributing to both the somatopl ...
... splanchnopleure during early extraembryonic mesoderm patternand hemangioblasts. Wolff (1764 Theorie von der generation) is ing. Among these three lineages, progenitors for the smooth generally credited for the earliest description of the blood islands. muscle cells, contributing to both the somatopl ...
Stable benefit of embryonic stem cell therapy in - AJP
... Pucéat, and Andre Terzic. Stable benefit of embryonic stem cell therapy in myocardial infarction. Am J Physiol Heart Circ Physiol 287: H471–H479, 2004; 10.1152/ajpheart.01247.2003.—Conventional therapies for myocardial infarction attenuate disease progression without contributing significantly to r ...
... Pucéat, and Andre Terzic. Stable benefit of embryonic stem cell therapy in myocardial infarction. Am J Physiol Heart Circ Physiol 287: H471–H479, 2004; 10.1152/ajpheart.01247.2003.—Conventional therapies for myocardial infarction attenuate disease progression without contributing significantly to r ...
CD1 Proteins, but No Functional CD1d The Bovine CD1 Family
... been elucidated. Because SBU-T6 was shown to immunoprecipitate sheep CD1e, and at least one other protein (18, 22), it is regarded as a pan-CD1-specific Ab. CC20 and BCD1b3 are known to recognize human CD1b. Our data, summarized in Table II, show that SBU-T6 recognizes thymocytes, peripheral B cells ...
... been elucidated. Because SBU-T6 was shown to immunoprecipitate sheep CD1e, and at least one other protein (18, 22), it is regarded as a pan-CD1-specific Ab. CC20 and BCD1b3 are known to recognize human CD1b. Our data, summarized in Table II, show that SBU-T6 recognizes thymocytes, peripheral B cells ...
A family of abundant plasma membrane
... Bound antibody was visualized with a 2,000× dilution of the rabbit horseradish peroxidase-conjugated anti-rat lg antiserum described for immunoblotting, but for ELISA tetramethylbenzidine was used in the developing solution. In certain instances the immobilized membranes were treated before addition ...
... Bound antibody was visualized with a 2,000× dilution of the rabbit horseradish peroxidase-conjugated anti-rat lg antiserum described for immunoblotting, but for ELISA tetramethylbenzidine was used in the developing solution. In certain instances the immobilized membranes were treated before addition ...
DCE
... • Applications identify resources by name, without needing to know where the resources are located • DCE cells can also participate in a worldwide directory service using the DCE Global Directory (GDS), which is based on the X.500 standard, or the Internet-system Domain Name Service (DNS) ...
... • Applications identify resources by name, without needing to know where the resources are located • DCE cells can also participate in a worldwide directory service using the DCE Global Directory (GDS), which is based on the X.500 standard, or the Internet-system Domain Name Service (DNS) ...
The Abundance of Cell Cycle Regulatory Protein Cdc4p Is
... tion site. The PCR products were cleaved with the appropriate enzymes and ligated into pEG(KG), which had previously been digested with the appropriate enzymes. Table 1 lists primers used in this study. Table 2 lists the constructs derived from using different sets of primers, which are numbered to ...
... tion site. The PCR products were cleaved with the appropriate enzymes and ligated into pEG(KG), which had previously been digested with the appropriate enzymes. Table 1 lists primers used in this study. Table 2 lists the constructs derived from using different sets of primers, which are numbered to ...
- ESAIM: Mathematical Modelling and Numerical
... Remark 1. Functions P, C, Z, A and most functions in the following will be considered as functions of time t and of i the infusion law (which is also itself a function of time), however we will omit to note the i-dependency whenever there is no ambiguity, hence writing A or A(t) rather than A(i, t). ...
... Remark 1. Functions P, C, Z, A and most functions in the following will be considered as functions of time t and of i the infusion law (which is also itself a function of time), however we will omit to note the i-dependency whenever there is no ambiguity, hence writing A or A(t) rather than A(i, t). ...
Prm1p, a Pheromone-regulated Multispanning Membrane Protein
... assembles from subunits associated with different bilayers— their final structures bear remarkable similarities (Weber et al., 1998). In each case, the assembled fusase has domains in- ...
... assembles from subunits associated with different bilayers— their final structures bear remarkable similarities (Weber et al., 1998). In each case, the assembled fusase has domains in- ...
University of Groningen Bromodeoxyuridine does not contribute to
... rates, we first established baseline SCE rates in both normal and BS cells. We used primary fibroblasts and EBVtransformed lymphoblasts from healthy donors and BS patients for our analysis. Logarithmically growing cells were pulsed with 40 M for one cell division (18 h for fibroblasts, 24 h for lym ...
... rates, we first established baseline SCE rates in both normal and BS cells. We used primary fibroblasts and EBVtransformed lymphoblasts from healthy donors and BS patients for our analysis. Logarithmically growing cells were pulsed with 40 M for one cell division (18 h for fibroblasts, 24 h for lym ...
pcDNA™3.1(+) - Thermo Fisher Scientific
... proper initiation of translation (Kozak, 1987; Kozak, 1991; Kozak, 1990). An example of a Kozak consensus sequence is provided below. Other sequences are possible, but the G or A at position –3 and the G at position +4 (shown in bold) illustrates the most commonly occurring sequence with strong cons ...
... proper initiation of translation (Kozak, 1987; Kozak, 1991; Kozak, 1990). An example of a Kozak consensus sequence is provided below. Other sequences are possible, but the G or A at position –3 and the G at position +4 (shown in bold) illustrates the most commonly occurring sequence with strong cons ...
Cleavage Furrow Establishment—A Preliminary to Cylindrical
... SYNOPSIS: The predictable pattern of cell shape changes characterizing animal development must be a consequence of control mechanisms that are at least analogous to those operating in dividing cells. When cells change shape by an internal mechanism, it is implied that they also contain systems that ...
... SYNOPSIS: The predictable pattern of cell shape changes characterizing animal development must be a consequence of control mechanisms that are at least analogous to those operating in dividing cells. When cells change shape by an internal mechanism, it is implied that they also contain systems that ...
Fig. - Journal of Cell Science
... KEY WORDS: Ubiquitin, Hedgehog, Gli1, PKA, Phosphorylation, DUB ...
... KEY WORDS: Ubiquitin, Hedgehog, Gli1, PKA, Phosphorylation, DUB ...
LIGAND-TARGETED THERAPEUTICS IN ANTICANCER THERAPY
... therapeutic intracellularly, lead to cytotoxicity (for example, immunoliposomes and immunotoxins). b | Binding of LTTs linked to noninternalizing antibodies will result in the LTT remaining attached at the target-cell surface (for example, ADEPT (antibody-directed enzyme–prodrug therapy)). c | All t ...
... therapeutic intracellularly, lead to cytotoxicity (for example, immunoliposomes and immunotoxins). b | Binding of LTTs linked to noninternalizing antibodies will result in the LTT remaining attached at the target-cell surface (for example, ADEPT (antibody-directed enzyme–prodrug therapy)). c | All t ...
Final presentation
... • They had not taken in the plasmid containing the Kanamycin resistance gene • They would die on the agar plates, because they were not resistant to the Kanamycin antibiotic The transformation process may not have been successful because: • Competent cells may not have been effective • Heat shock pr ...
... • They had not taken in the plasmid containing the Kanamycin resistance gene • They would die on the agar plates, because they were not resistant to the Kanamycin antibiotic The transformation process may not have been successful because: • Competent cells may not have been effective • Heat shock pr ...
MHC antigeny
... Cross-presentation of antigen • MHC class I molecules are able to present exogenous peptides (cross-presentation) • Material recognised by cell surface receptors is endocyted, become a part of endosomes, where is partially (but not completely) degraded and transported into cytoplasm, where is cleav ...
... Cross-presentation of antigen • MHC class I molecules are able to present exogenous peptides (cross-presentation) • Material recognised by cell surface receptors is endocyted, become a part of endosomes, where is partially (but not completely) degraded and transported into cytoplasm, where is cleav ...
Oxidation of Hg(0) - Department of Marine and Coastal Sciences
... the presence of Hg(II) using the method of Bloom (1989). Ethylation experiments were performed by adding sodium tetraethylborate to samples that were not pre-oxidized with BrCl. Because tetraethylborate reacts with Hg(II), but not Hg(0), to form diethylmercury (Rapsomanikis et al., 1986), the detect ...
... the presence of Hg(II) using the method of Bloom (1989). Ethylation experiments were performed by adding sodium tetraethylborate to samples that were not pre-oxidized with BrCl. Because tetraethylborate reacts with Hg(II), but not Hg(0), to form diethylmercury (Rapsomanikis et al., 1986), the detect ...
G Protein Subunits Synthesized in Sf9 Cells
... Cell Culture and Cloning of Recombinant Baculouiruses-Fall armyworm ovary (Sf9)cells were propagated in IPL-41 medium supplemented with 10% fetal calf serum as described (34). Recombinant baculoviruses were generated by cotransfection of Sf9 cells with the expression vectors described above and with ...
... Cell Culture and Cloning of Recombinant Baculouiruses-Fall armyworm ovary (Sf9)cells were propagated in IPL-41 medium supplemented with 10% fetal calf serum as described (34). Recombinant baculoviruses were generated by cotransfection of Sf9 cells with the expression vectors described above and with ...
Come in and take your coat off how host cells
... engage receptors and mediate fusion events with cellular membranes (Harrison, 2008). Virus structures are stable – crosslinked by networks of intermolecular interactions to withstand hostile conditions encountered in the extracellular space. In fact, virus structures are metastable – poised upon cel ...
... engage receptors and mediate fusion events with cellular membranes (Harrison, 2008). Virus structures are stable – crosslinked by networks of intermolecular interactions to withstand hostile conditions encountered in the extracellular space. In fact, virus structures are metastable – poised upon cel ...
Extended Survivability of Prostate Cancer Cells
... transduction modifiers such as phorbol esters (30-32) and thapsigargin (33). We recently reported apoptosis induction by prostate secre tory protein 94 (34) and hydroxamic acid compounds (35, 36) in hormone-refractory human prostate cancer cells. Little information exists in regard to differential p ...
... transduction modifiers such as phorbol esters (30-32) and thapsigargin (33). We recently reported apoptosis induction by prostate secre tory protein 94 (34) and hydroxamic acid compounds (35, 36) in hormone-refractory human prostate cancer cells. Little information exists in regard to differential p ...
MHC antigeny
... Cross-presentation of antigen • MHC class I molecules are able to present exogenous peptides (cross-presentation) • Material recognised by cell surface receptors is endocyted, become a part of endosomes, where is partially (but not completely) degraded and transported into cytoplasm, where is cleav ...
... Cross-presentation of antigen • MHC class I molecules are able to present exogenous peptides (cross-presentation) • Material recognised by cell surface receptors is endocyted, become a part of endosomes, where is partially (but not completely) degraded and transported into cytoplasm, where is cleav ...
A Role for Eosinophils in Adaptive Humoral Immunity
... arginine molecules which attach to plasma membranes, destabilizing the lipid bilayers and increasing their permeability, presumably by forming ion channels [42] and/or by membrane depolarization. RNase-3 also acts to inhibit further growth of cells without being catalytic. RNase-2 is composed of few ...
... arginine molecules which attach to plasma membranes, destabilizing the lipid bilayers and increasing their permeability, presumably by forming ion channels [42] and/or by membrane depolarization. RNase-3 also acts to inhibit further growth of cells without being catalytic. RNase-2 is composed of few ...
Development of differentiation assay in neuroblastoma to elucidate
... overall survival of only 50%. Amplification of MYCN and overexpression of its coded protein is associated with rapid tumour progression and poor outcome. Induction of terminal differentiation is a very promising approach to neuroblastoma treatment. However, there’s no golden standard to asses neurob ...
... overall survival of only 50%. Amplification of MYCN and overexpression of its coded protein is associated with rapid tumour progression and poor outcome. Induction of terminal differentiation is a very promising approach to neuroblastoma treatment. However, there’s no golden standard to asses neurob ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.