View
... • Production of a magnified image of the lamp filament that is focused at the level of the aperture plane of the condensor. • Opening or closing this diaphragm controls the angle of the light cone that reaches the specimen, which is determining the image resolution along with the numerical aperture ...
... • Production of a magnified image of the lamp filament that is focused at the level of the aperture plane of the condensor. • Opening or closing this diaphragm controls the angle of the light cone that reaches the specimen, which is determining the image resolution along with the numerical aperture ...
Click To
... replaces a single focal plane array detector with two focal plane array detectors orientated with respect to each other such that a power splitter divides an incoming light source equally between each detector. ...
... replaces a single focal plane array detector with two focal plane array detectors orientated with respect to each other such that a power splitter divides an incoming light source equally between each detector. ...
MPE Tutorial Multiphoton Excitation Microscopy 5100 Patrick Henry Drive
... When working with biological samples, serious problems can occur with normal confocal fluorescence microscopy. One problem is photobleaching of the fluorescent label (fluorophore). In many cases, researchers are interested in observing living specimens, often at several stages during development. Be ...
... When working with biological samples, serious problems can occur with normal confocal fluorescence microscopy. One problem is photobleaching of the fluorescent label (fluorophore). In many cases, researchers are interested in observing living specimens, often at several stages during development. Be ...
Raman Spectra of Optically Trapped Microcomplexes
... from different planes is mostly suppressed by the pinhole; signal-to-noise-ratio (SNR) increases; scans from different layers and depths may be recorded separately. In vivo Raman scanning of transparent tissues (eyes). ...
... from different planes is mostly suppressed by the pinhole; signal-to-noise-ratio (SNR) increases; scans from different layers and depths may be recorded separately. In vivo Raman scanning of transparent tissues (eyes). ...
Generalizing the Confocal Microscope via Heterodyne Interferometry and Digital Filtering
... It is in this regard—2π phase sensitivity, 0.1-nm height accuracy, high speed, and completely arbitrary point spread function (within the bandwidth limit)—that we claim this system to be a true generalization of the confocal microscope. ...
... It is in this regard—2π phase sensitivity, 0.1-nm height accuracy, high speed, and completely arbitrary point spread function (within the bandwidth limit)—that we claim this system to be a true generalization of the confocal microscope. ...
Light Sources
... You can think of the aperture as truncating these diffracted orders at some small number. • The value of sin a for an optical system is the numerical aperture, or NA. If the value of the NA is small for a system, fewer orders will be imaged, and the ...
... You can think of the aperture as truncating these diffracted orders at some small number. • The value of sin a for an optical system is the numerical aperture, or NA. If the value of the NA is small for a system, fewer orders will be imaged, and the ...
Biology 177: Principles of Modern Microscopy
... technique so novel compared to all the others? Hint: see this figure from Ke, M.-T., Fujimoto, S., Imai, T., 2013. Nat Neurosci 16, 1154-1161. ...
... technique so novel compared to all the others? Hint: see this figure from Ke, M.-T., Fujimoto, S., Imai, T., 2013. Nat Neurosci 16, 1154-1161. ...
Optical microscope - Frank`s Hospital Workshop
... Digital microscopes Low power microscopy is also possible with digital microscopes, with a camera attached directly to the USB port of a computer, so that the images are shown directly on the monitor. Often called "USB" microscopes, they offer high magnifications (up to about 200×) without the need ...
... Digital microscopes Low power microscopy is also possible with digital microscopes, with a camera attached directly to the USB port of a computer, so that the images are shown directly on the monitor. Often called "USB" microscopes, they offer high magnifications (up to about 200×) without the need ...
m5zn_aa487bab657cf4d
... nutrients to the soil, Nitrates, Phosphates etc) Saprophytes or decomposers are organisms that live on dead and/or decaying organic matter, source of nutrients and some carry out photosynthesis, Photosynthetic algae and bacteria (such as cyanobacteria) produce much of the oxygen in our atmosphere (m ...
... nutrients to the soil, Nitrates, Phosphates etc) Saprophytes or decomposers are organisms that live on dead and/or decaying organic matter, source of nutrients and some carry out photosynthesis, Photosynthetic algae and bacteria (such as cyanobacteria) produce much of the oxygen in our atmosphere (m ...
Quantum Computation using Photons
... important role not only in life sciences but also material sciences. In the 20th century, various kinds of unique microscopic techniques have been developed such as phase contrast microscopy, electron microscopy, scanning probe microscopy, and near-field scanning optical microscopy. Among them, opti ...
... important role not only in life sciences but also material sciences. In the 20th century, various kinds of unique microscopic techniques have been developed such as phase contrast microscopy, electron microscopy, scanning probe microscopy, and near-field scanning optical microscopy. Among them, opti ...
File - Science with Mr. Davis
... light striking the object being studied. It is critical that you know the proper use of the condenser and iris diaphragm. A common problem with microscope use is having too much light, which obliterates the object (more or less like trying to see something while looking directly at the sun). You wil ...
... light striking the object being studied. It is critical that you know the proper use of the condenser and iris diaphragm. A common problem with microscope use is having too much light, which obliterates the object (more or less like trying to see something while looking directly at the sun). You wil ...
Biology Outline Dec 1-5
... compare the function and uses of types of microscopes identify and describe the role of each part of the microscope describe how to properly carry, focus and store a microscope define the terms: size of field of view, magnification, resolution, contrast use the term magnification, resolution and con ...
... compare the function and uses of types of microscopes identify and describe the role of each part of the microscope describe how to properly carry, focus and store a microscope define the terms: size of field of view, magnification, resolution, contrast use the term magnification, resolution and con ...
Lab 2: Abbe Theory of Imaging
... Carry out experiments in sequence with square mesh object as shown in Table 2. Place the square mesh in vertical orientation. Mark the locations of the Fourier image dots which are on the x- and y-axis with a white paper pasted on an index card. These locations will be useful to make various spatial ...
... Carry out experiments in sequence with square mesh object as shown in Table 2. Place the square mesh in vertical orientation. Mark the locations of the Fourier image dots which are on the x- and y-axis with a white paper pasted on an index card. These locations will be useful to make various spatial ...
Viewing Cells Microscopes are used to magnify cells. The number of
... tiny glass bead for a lens. With it, he reported seeing things in pond water that no one had ever imagined. His microscope could magnify up the image of an object 270 times larger than its actual size. Today you would say his lens has a power of 270x. Early compound microscopes were crude by today’s ...
... tiny glass bead for a lens. With it, he reported seeing things in pond water that no one had ever imagined. His microscope could magnify up the image of an object 270 times larger than its actual size. Today you would say his lens has a power of 270x. Early compound microscopes were crude by today’s ...
preprint version PDF - Emory Physics Department
... conventional “snapshot” camera or a CCD video camera. The latter is now more common, and well-suited for studying samples which move or change. The output of the CCD video camera is usually attached to a frame grabber card in a computer, so that data is saved digitally, although a conventional VCR ...
... conventional “snapshot” camera or a CCD video camera. The latter is now more common, and well-suited for studying samples which move or change. The output of the CCD video camera is usually attached to a frame grabber card in a computer, so that data is saved digitally, although a conventional VCR ...
Imaging Laboratory Exercise Scanning Electron Microscope
... metal, such as gold, before it is imaged in the microscope. Because of this, these devices have a limited application, especially for examining dynamic samples, such as in life forms. In both SEM and TEM electrons are generated in the portion of the microscope that is referred to as the electron gun ...
... metal, such as gold, before it is imaged in the microscope. Because of this, these devices have a limited application, especially for examining dynamic samples, such as in life forms. In both SEM and TEM electrons are generated in the portion of the microscope that is referred to as the electron gun ...
Live Data Mode
... A major challenge in physiological applications is the study of cells in deeper layers of tissue. Multiphoton microscopy provides several advantages to solve this challenge. Lower light scattering, restricted excitation and bleaching to the focal plane, and reduced phototoxicity are the properties o ...
... A major challenge in physiological applications is the study of cells in deeper layers of tissue. Multiphoton microscopy provides several advantages to solve this challenge. Lower light scattering, restricted excitation and bleaching to the focal plane, and reduced phototoxicity are the properties o ...
Two laser wavelength Thomson Scattering for high electron
... relativistic blue shift of the spectrum are the causes of inadmissible error bars. Due to background radiation (line emission and Bremsstrahlung) it is not advisable to extend the interference filters of the polychromators to much shorter wavelength. As an alternative method an additional Nd:YAG las ...
... relativistic blue shift of the spectrum are the causes of inadmissible error bars. Due to background radiation (line emission and Bremsstrahlung) it is not advisable to extend the interference filters of the polychromators to much shorter wavelength. As an alternative method an additional Nd:YAG las ...
cameras - Purdue Engineering
... focusing, like a lens. There can be more than 2 elements. Sometimes there are flat mirrors to just “fold” the optical path for a needed long focal length. Area CCD arrays are not big enough for practical use. Dimension of linear array determines image “width” and cross-track GSD, orbit motion or “bo ...
... focusing, like a lens. There can be more than 2 elements. Sometimes there are flat mirrors to just “fold” the optical path for a needed long focal length. Area CCD arrays are not big enough for practical use. Dimension of linear array determines image “width” and cross-track GSD, orbit motion or “bo ...
Material Characterization
... A scanning tunneling microscope (STM) is a powerful instrument for imaging surfaces at the atomic level. Its development in 1981 earned its inventors, Gerd Binnig and Heinrich Rohrer (at IBM Zürich), the Nobel Prize in Physics in 1986. For an STM, good resolution is considered to be 0.1 nm lateral r ...
... A scanning tunneling microscope (STM) is a powerful instrument for imaging surfaces at the atomic level. Its development in 1981 earned its inventors, Gerd Binnig and Heinrich Rohrer (at IBM Zürich), the Nobel Prize in Physics in 1986. For an STM, good resolution is considered to be 0.1 nm lateral r ...
Optical laser beam scanner lens relay system
... with standard, i.e. commonly available lenses. Nevertheless, a very good performance can be obtained with a little bit of care in the design. Even a very simple design using achromats is adequate, although significantly improved performance can be obtained if meniscus lenses are added. The general r ...
... with standard, i.e. commonly available lenses. Nevertheless, a very good performance can be obtained with a little bit of care in the design. Even a very simple design using achromats is adequate, although significantly improved performance can be obtained if meniscus lenses are added. The general r ...
Get PDF - OSA Publishing
... imaging technique, dark-field (DF) microscopy is a spectacular method to form high-contrast images of unstained transparent specimens. In order to form a bright specimen image on a dark background, oblique rays from every azimuth are allowed to strike the samples, but only light scattered from the s ...
... imaging technique, dark-field (DF) microscopy is a spectacular method to form high-contrast images of unstained transparent specimens. In order to form a bright specimen image on a dark background, oblique rays from every azimuth are allowed to strike the samples, but only light scattered from the s ...
equation
... with ðI0 =Isat Þ3=2 , and for a given Isat on the intensity I0 that can be tolerated by the sample. Thus, in RESOLFT microscopy, the resolution is no longer limited by the wavelength l, but by the applicable light intensity I at a given Isat that is specific for the label. If we now assume that stat ...
... with ðI0 =Isat Þ3=2 , and for a given Isat on the intensity I0 that can be tolerated by the sample. Thus, in RESOLFT microscopy, the resolution is no longer limited by the wavelength l, but by the applicable light intensity I at a given Isat that is specific for the label. If we now assume that stat ...
test
... You are asked to design a pinhole camera with Gaussian amplitude apodization in the pinhole. So, rather than a perfectly clear circular “hole” for a pinhole, the “hole” is a piece of material which has increasing attenuation (less than unity amplitude transmission) as the distance from the center of ...
... You are asked to design a pinhole camera with Gaussian amplitude apodization in the pinhole. So, rather than a perfectly clear circular “hole” for a pinhole, the “hole” is a piece of material which has increasing attenuation (less than unity amplitude transmission) as the distance from the center of ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.