Peeping in on the cytoskeleton: light microscopy
... dependent on the scattering of the excitation and emission wavelengths by the specimen, and the absorption of the excitation energy along the entire beam path. This large excitation volume results in photobleaching and phototoxicity throughout the entire sample. Two-photon excitation or two-photon c ...
... dependent on the scattering of the excitation and emission wavelengths by the specimen, and the absorption of the excitation energy along the entire beam path. This large excitation volume results in photobleaching and phototoxicity throughout the entire sample. Two-photon excitation or two-photon c ...
Biology 177: Principles of Modern Microscopy
... • Practically zero working distance and small depth of field. • Extremely long scan times for high resolution images or large specimen areas. ...
... • Practically zero working distance and small depth of field. • Extremely long scan times for high resolution images or large specimen areas. ...
PPT Version - OMICS International
... 1. femtosecond laser is expensive (1 μm) 2. transverse resolution needs to be similar to axial resolution, below 10 μm need short confocal parameter which results in the focus falling off rapidly. Acquisition rate: <10franes/second Lack of large-scale clinical trials ...
... 1. femtosecond laser is expensive (1 μm) 2. transverse resolution needs to be similar to axial resolution, below 10 μm need short confocal parameter which results in the focus falling off rapidly. Acquisition rate: <10franes/second Lack of large-scale clinical trials ...
repeat
... For each of the molecules shown below, CLEARLY draw an asterisk next to each stereocentre. Then indicate under each molecule whether it is CHIRAL or ACHIRAL. ...
... For each of the molecules shown below, CLEARLY draw an asterisk next to each stereocentre. Then indicate under each molecule whether it is CHIRAL or ACHIRAL. ...
Diffraction
... focus, any spatial variations in the beam (dashed lines in figure) will not pass through the pinhole. (Note that when the objective is highly defocused, the wave fronts entering the pinhole are closer to planar than to spherical. We will use this in several sections.) The filtered light leaving the ...
... focus, any spatial variations in the beam (dashed lines in figure) will not pass through the pinhole. (Note that when the objective is highly defocused, the wave fronts entering the pinhole are closer to planar than to spherical. We will use this in several sections.) The filtered light leaving the ...
Resolution questions with solutions
... In this experiment, the light from point source P has a wavelength of 500 nm and the width of the central maximum of intensity on the screen is 10.0 mm. When light of unknown wavelength λ is used, the width of the central maximum of intensity is 13.0 mm. Determine the value of λ. ...
... In this experiment, the light from point source P has a wavelength of 500 nm and the width of the central maximum of intensity on the screen is 10.0 mm. When light of unknown wavelength λ is used, the width of the central maximum of intensity is 13.0 mm. Determine the value of λ. ...
Opto-acoustic Imaging
... other words, the illuminating laser beam and the reflected beam both traverse the medium through which the ultrasound is traveling. Hence, in addition to the optical path length change induced by the motion of the reflecting surface, a change also results from the variation of refractive index with ...
... other words, the illuminating laser beam and the reflected beam both traverse the medium through which the ultrasound is traveling. Hence, in addition to the optical path length change induced by the motion of the reflecting surface, a change also results from the variation of refractive index with ...
2011 Research Poster
... the optical trap, we diffract atoms by passing a moving standing wave through the condensate. The standing wave acts as a diffraction grating that diffracts atoms, giving them a momentum kick as first or higher order diffractions of the condensate. We split the beam into two parts by the polarizing ...
... the optical trap, we diffract atoms by passing a moving standing wave through the condensate. The standing wave acts as a diffraction grating that diffracts atoms, giving them a momentum kick as first or higher order diffractions of the condensate. We split the beam into two parts by the polarizing ...
Jannick Rolland, PhD
... CenterFreeformOptics.org), the R.E. Hopkins Center (www.hopkinscenter.rochester.edu), and the ODALab (www.odalabspectrum.org). She graduated from the optical engineering school of the Institut d'Optique Théorique et Appliquée, France, and earned a PhD from the College of Optical Sciences at the Univ ...
... CenterFreeformOptics.org), the R.E. Hopkins Center (www.hopkinscenter.rochester.edu), and the ODALab (www.odalabspectrum.org). She graduated from the optical engineering school of the Institut d'Optique Théorique et Appliquée, France, and earned a PhD from the College of Optical Sciences at the Univ ...
DYNAMICS OF THE CELL MEMBRANE OBSERVED UNDER THE
... illumination. All of these properties combined together, it is the most advantageous lens for the microscopy with an evanescent wave illumination or total internal reflection fluorescence microscopy (TIRFM)'\ A disadvantage is that one has to use the lens in combination with specially selected oil a ...
... illumination. All of these properties combined together, it is the most advantageous lens for the microscopy with an evanescent wave illumination or total internal reflection fluorescence microscopy (TIRFM)'\ A disadvantage is that one has to use the lens in combination with specially selected oil a ...
Hamamatsu Camera Product Hightlights
... The ORCA-285 is a high resolution digital camera using a progressive scan interline CCD chip with no mechanical shutter. In addition to a high resolution of 1.37 million pixels, a wide dynamic range of 12 bit digital output and high sensitivity offers a wide application range down to low light level ...
... The ORCA-285 is a high resolution digital camera using a progressive scan interline CCD chip with no mechanical shutter. In addition to a high resolution of 1.37 million pixels, a wide dynamic range of 12 bit digital output and high sensitivity offers a wide application range down to low light level ...
UV-light microscope: improvements in optical imaging for a
... components that did not transmit UV light were replaced with UV compatible versions, including the optical waveguide, the objective lens, the mirror and the transfer lens inside the vacuum chamber; as well as the condenser lens of the illuminator and the zoom lens below the CCD camera that are outsi ...
... components that did not transmit UV light were replaced with UV compatible versions, including the optical waveguide, the objective lens, the mirror and the transfer lens inside the vacuum chamber; as well as the condenser lens of the illuminator and the zoom lens below the CCD camera that are outsi ...
Fluorescence Microscopy
... phenomena, and where better its application than when trying to comprehend cell physiology using florescence microscopy? Microscopy has played an important role in determining the activity of cells, from the very early appreciation of living animalcules by Van Leeuwenhoek (Ford 1989) with a simple mi ...
... phenomena, and where better its application than when trying to comprehend cell physiology using florescence microscopy? Microscopy has played an important role in determining the activity of cells, from the very early appreciation of living animalcules by Van Leeuwenhoek (Ford 1989) with a simple mi ...
abstract - Med-e-Tel
... based on high-content, automated, light microscopy, was developed. The reader allows fast capture of high resolution (down to 0.35 µm) microarray images using a large range of fluorescent dyes in the 380 – 700 nm range, also allowing up to 4 simultaneous labels. Controlled by high performance softwa ...
... based on high-content, automated, light microscopy, was developed. The reader allows fast capture of high resolution (down to 0.35 µm) microarray images using a large range of fluorescent dyes in the 380 – 700 nm range, also allowing up to 4 simultaneous labels. Controlled by high performance softwa ...
Final Exam
... its mirrors (mirror 1 in Figure below) is moved by 25 µm, and it is observed that 89 fringe-pairs, bright and dark, pass by the detector during this process. Determine the wavelength of the light beam. ...
... its mirrors (mirror 1 in Figure below) is moved by 25 µm, and it is observed that 89 fringe-pairs, bright and dark, pass by the detector during this process. Determine the wavelength of the light beam. ...
Microscopy Chapter 1
... Electron Microscopes – use a beam of electrons which are either passed through or bounced off of a specimen Types include: ...
... Electron Microscopes – use a beam of electrons which are either passed through or bounced off of a specimen Types include: ...
James Powenski - Optical Computing
... substrate to make Thin film waveguides. n Mirrors can be simulated by using diffraction grating. ...
... substrate to make Thin film waveguides. n Mirrors can be simulated by using diffraction grating. ...
Lecture 3
... http://micro.magnet.fsu.edu/primer/java/darkfield/cardioid/index.html http://micro.magnet.fsu.edu/primer/techniques/darkfieldreflect.html reflected DF ...
... http://micro.magnet.fsu.edu/primer/java/darkfield/cardioid/index.html http://micro.magnet.fsu.edu/primer/techniques/darkfieldreflect.html reflected DF ...
THINK ABOUT IT
... Electron microscopes use beams of electrons, not light, that are focused by magnetic fields. Electron microscopes offer much higher resolution than light microscopes. There are two major types of electron microscopes: transmission and scanning. ...
... Electron microscopes use beams of electrons, not light, that are focused by magnetic fields. Electron microscopes offer much higher resolution than light microscopes. There are two major types of electron microscopes: transmission and scanning. ...
Low-Cost Tunable Adaptive Optics.pdf
... beam. Bessel beams are nondiffracting and self-healing. Because of these properties, they have uses in optical micromanipulation, where they can form two dimensional traps and transport microscopic particles over long distances. Bessel beams are also used in laser materials processing to process une ...
... beam. Bessel beams are nondiffracting and self-healing. Because of these properties, they have uses in optical micromanipulation, where they can form two dimensional traps and transport microscopic particles over long distances. Bessel beams are also used in laser materials processing to process une ...
Correlative Fluorescence and Electron Microscopy on Ultrathin
... be reduced (see above). Third, correlative microscopy is facilitated. This brings additional resolution and, importantly, the ability to confirm that a structure seen by light microscopy is the same as another seen by electron microscopy. The two different preparative procedures we have employed are ...
... be reduced (see above). Third, correlative microscopy is facilitated. This brings additional resolution and, importantly, the ability to confirm that a structure seen by light microscopy is the same as another seen by electron microscopy. The two different preparative procedures we have employed are ...
Tao Yuan, Jingzhou Xu, and Xicheng Zhang Rensselaer
... Criterion. The limitation is more serious for THz image system because of the long wavelength used (~300 m for 1THz). Sub-wavelength resolution can be achieved by near field technology shown in figure 1 (b) and (c), which are subwavelength aperture and aperture-less method. In apertureless method, ...
... Criterion. The limitation is more serious for THz image system because of the long wavelength used (~300 m for 1THz). Sub-wavelength resolution can be achieved by near field technology shown in figure 1 (b) and (c), which are subwavelength aperture and aperture-less method. In apertureless method, ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.