A short introduction to light and electron microscopy
... spot. The spot is scanned in a raster fashion over the sample to illuminate fluorescent dyes. Emission of fluorescent light is detected through a pinhole located in a confocal plane relative to the plane of focus. Stray light from structures out of focus is effectively blocked by the pinhole leading ...
... spot. The spot is scanned in a raster fashion over the sample to illuminate fluorescent dyes. Emission of fluorescent light is detected through a pinhole located in a confocal plane relative to the plane of focus. Stray light from structures out of focus is effectively blocked by the pinhole leading ...
Topic 2: Microscopy and Staining Measurement of Microorganisms
... length: distance between centre of lens and focal point. Microscope lens effectively decreases the focal length. The shorter the effective focal length, the larger the object appears. Microscope magnifies image by decreasing focal length. Resolution: The minimum distance (d) between two objects that ...
... length: distance between centre of lens and focal point. Microscope lens effectively decreases the focal length. The shorter the effective focal length, the larger the object appears. Microscope magnifies image by decreasing focal length. Resolution: The minimum distance (d) between two objects that ...
Super-resolution Microscopy
... can be broadly categorized into two main approaches [1]. In the first approach, called “targeted switching and readout”, the illumination volume in a fluorescent sample is confined to a small region, which is much smaller than the diffraction-limited spot size. Stimulated emission depletion (STED) m ...
... can be broadly categorized into two main approaches [1]. In the first approach, called “targeted switching and readout”, the illumination volume in a fluorescent sample is confined to a small region, which is much smaller than the diffraction-limited spot size. Stimulated emission depletion (STED) m ...
Microscopy
... DIC microscopy uses polarizing optics to represent the phase difference across the shear direction. Although the “relief” style image in DIC has weaker contrast compared to phase contrast, it benefits from marginally higher resolution, and doesn’t have the “halo” artifact typical of phase contrast. ...
... DIC microscopy uses polarizing optics to represent the phase difference across the shear direction. Although the “relief” style image in DIC has weaker contrast compared to phase contrast, it benefits from marginally higher resolution, and doesn’t have the “halo” artifact typical of phase contrast. ...
Robert Jones
... Autoradiography with 125I-alpha-bungarotoxin (Bgt) and 3H-methyllycaconitine were used to map the general distribution of alpha7 nAChR within the rat PFC. AlexaFluor 488-conjugated Bgt in conjunction with other neuronal markers provided further analysis of regions of interest at higher magnificatio ...
... Autoradiography with 125I-alpha-bungarotoxin (Bgt) and 3H-methyllycaconitine were used to map the general distribution of alpha7 nAChR within the rat PFC. AlexaFluor 488-conjugated Bgt in conjunction with other neuronal markers provided further analysis of regions of interest at higher magnificatio ...
Comparison of laser scanning methods
... Beam steering (x-y) • An acousto-optics deflector changes the angle of direction of a laser beam and its angular position is linearly proportional to the acoustic frequency, so that the higher the frequency, the larger the diffracted angle. ...
... Beam steering (x-y) • An acousto-optics deflector changes the angle of direction of a laser beam and its angular position is linearly proportional to the acoustic frequency, so that the higher the frequency, the larger the diffracted angle. ...
Lecture 18
... Holography versus photography • Records light from many directions not just one • Requires laser, can’t use normal light sources • No need for a lens • Needs second beam to see (reconstruction beam) • Requires specific illumination to see • Cut in half, see two of same image not half of it • More 3 ...
... Holography versus photography • Records light from many directions not just one • Requires laser, can’t use normal light sources • No need for a lens • Needs second beam to see (reconstruction beam) • Requires specific illumination to see • Cut in half, see two of same image not half of it • More 3 ...
Point spread function engineering in confocal scanning microscopy
... The improvement of the performance of optical microscopes has aimed many researches along the last decades. Conventional wide-field microscopes are close to reach their maximum efficiency through the use of the available objectives with numerical apertures (NA) up to 1.4. However, when using this ki ...
... The improvement of the performance of optical microscopes has aimed many researches along the last decades. Conventional wide-field microscopes are close to reach their maximum efficiency through the use of the available objectives with numerical apertures (NA) up to 1.4. However, when using this ki ...
Title
... inclusion bodies in cells. These are believed to result from misfolding of the poly-Q proteins in addition to the formation of beta-amyloid protein within the bodies. It is assumed that the accumulation of inclusion bodies in neurons results in neurodegeneration and clinical symptoms, however this l ...
... inclusion bodies in cells. These are believed to result from misfolding of the poly-Q proteins in addition to the formation of beta-amyloid protein within the bodies. It is assumed that the accumulation of inclusion bodies in neurons results in neurodegeneration and clinical symptoms, however this l ...
Foundations in Microbiology - Houston Community College System
... Types of light microscopes • Bright-field – most widely used, specimen is darker than surrounding field • Dark-field – brightly illuminated specimens surrounded by dark field • Phase-contrast – transforms subtle changes in light waves passing through the specimen into differences in light intensity ...
... Types of light microscopes • Bright-field – most widely used, specimen is darker than surrounding field • Dark-field – brightly illuminated specimens surrounded by dark field • Phase-contrast – transforms subtle changes in light waves passing through the specimen into differences in light intensity ...
Looking at Cells - Harrison High School
... ______ 7. Which scientist used a microscope to view “animacules” in pond water? a. van Leeuwenhoek b. Hooke c. Schleiden d. Virchow ______ 8. A millimeter is equivalent to: a. about half the diameter of a Lincoln penny. b. a little more than a yard. c. about the width of a pencil tip. d. about the l ...
... ______ 7. Which scientist used a microscope to view “animacules” in pond water? a. van Leeuwenhoek b. Hooke c. Schleiden d. Virchow ______ 8. A millimeter is equivalent to: a. about half the diameter of a Lincoln penny. b. a little more than a yard. c. about the width of a pencil tip. d. about the l ...
Press Release Product Developed under CSIR
... Confocal Microscopes are used to obtain three dimensional features at microscopic level and play vital role in scientific understanding of nano‐ materials, biological objects etc. At present, these are only available at limited number of science laboratories in India due to the ...
... Confocal Microscopes are used to obtain three dimensional features at microscopic level and play vital role in scientific understanding of nano‐ materials, biological objects etc. At present, these are only available at limited number of science laboratories in India due to the ...
The Compound Microscope
... The Scanning Electron Microscope • Finally, the scanning electron microscope (SEM) bombards a specimen with a beam of electrons instead of light to produce a highly magnified image from 100x to 100,0000x. • Its depth of focus is some 300 times better than optical systems at similar magnification. • ...
... The Scanning Electron Microscope • Finally, the scanning electron microscope (SEM) bombards a specimen with a beam of electrons instead of light to produce a highly magnified image from 100x to 100,0000x. • Its depth of focus is some 300 times better than optical systems at similar magnification. • ...
Imaging live cells by X-ray laser diffraction - SPring-8
... Research Institute for Electronic Science, ...
... Research Institute for Electronic Science, ...
7.04 Stomatal movements - preparation
... suitable stage of growth. If the leaves are collected long before the experiment or on a sunny afternoon, the stomata may be closed. They can usually be induced to open again by covering the leaves with po1ythene bags and exposing to light. In the case of rhubarb, the leaves are cut and brought into ...
... suitable stage of growth. If the leaves are collected long before the experiment or on a sunny afternoon, the stomata may be closed. They can usually be induced to open again by covering the leaves with po1ythene bags and exposing to light. In the case of rhubarb, the leaves are cut and brought into ...
Optics and Optoelectronics
... eyepiece, telescopes, microscope. Thick lenses. Aspherical surfaces. 4. Light as an electromagnetic wave. Huygens’ principle. Photometry. Sources of optical ...
... eyepiece, telescopes, microscope. Thick lenses. Aspherical surfaces. 4. Light as an electromagnetic wave. Huygens’ principle. Photometry. Sources of optical ...
f Tools of the Laboratory: The 5 I's of culturing microbes Isolation
... Types of light microscopes • Bright-field – most widely used, specimen is darker than surrounding field • Dark-field – brightly illuminated p surrounded by y dark field specimens • Phase-contrast – transforms subtle changes in light waves passing through the specimen into differences in light intens ...
... Types of light microscopes • Bright-field – most widely used, specimen is darker than surrounding field • Dark-field – brightly illuminated p surrounded by y dark field specimens • Phase-contrast – transforms subtle changes in light waves passing through the specimen into differences in light intens ...
s1reproduction03 - skh chan young secondary school
... using a light microscope Experiment 3.2 and 3.3 Students are given chance to use the microscope to observe given specimen ...
... using a light microscope Experiment 3.2 and 3.3 Students are given chance to use the microscope to observe given specimen ...
Imaging the Division Process in Living Tissue Culture Cells
... including the development of confocal and wide-field deconvolution systems (12-14). These fluorescent approaches can also be combined with conventional transmitted imaging modes, like phase-contrast or differential interference contrast (DIC), to form 4-D “multimode” systems (15). This, in turn, al ...
... including the development of confocal and wide-field deconvolution systems (12-14). These fluorescent approaches can also be combined with conventional transmitted imaging modes, like phase-contrast or differential interference contrast (DIC), to form 4-D “multimode” systems (15). This, in turn, al ...
Microscope
... objectives are 4x, 10x, 40x, 100x, oil Total magnification is eye piece magnification multiplied times objective you are viewing with ...
... objectives are 4x, 10x, 40x, 100x, oil Total magnification is eye piece magnification multiplied times objective you are viewing with ...
MEDICAL BIOLOGY AND GENETICS 1 Comenius
... 10. What is the principal of transmission electron microscope? The T ransmission E lectron M icroscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light. Because the wavelength of electrons is much smaller than that of light, the optim ...
... 10. What is the principal of transmission electron microscope? The T ransmission E lectron M icroscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light. Because the wavelength of electrons is much smaller than that of light, the optim ...
Magnetic Resonance Imaging of Human High
... • The presence of low frequency modulation of both vascular (Hb, HbO) and metabolic responses to visual stimuli with unknown origin have been observed. • The cause of these low frequency oscillations are not exactly known • but may be attributed to extra-cerebral activities such as respiration. • O ...
... • The presence of low frequency modulation of both vascular (Hb, HbO) and metabolic responses to visual stimuli with unknown origin have been observed. • The cause of these low frequency oscillations are not exactly known • but may be attributed to extra-cerebral activities such as respiration. • O ...
Observing Microorganisms Through a Microscope
... and 1000. A magnification of 2000 times is about the highest obtainable. The specimen is illuminated by visible light from the light source—the illuminator—that is passed through a condenser, which directs the light rays through the specimen (Figure 3.1a). Resolution, or resolving power, is the abil ...
... and 1000. A magnification of 2000 times is about the highest obtainable. The specimen is illuminated by visible light from the light source—the illuminator—that is passed through a condenser, which directs the light rays through the specimen (Figure 3.1a). Resolution, or resolving power, is the abil ...
See Beyond Conventional Light Microscopy! Enter the World of
... SR-SIM: Superresolution structured illumination microscopy brings you up to twice the gain in resolution in all dimensions – without compromising on dyes, and without special sample treatment. Resolving small structures corresponds to detecting high spatial frequencies in your sample. A simple funda ...
... SR-SIM: Superresolution structured illumination microscopy brings you up to twice the gain in resolution in all dimensions – without compromising on dyes, and without special sample treatment. Resolving small structures corresponds to detecting high spatial frequencies in your sample. A simple funda ...
Zach Stephen Richard Worhatch Royce Grewer
... and opposite forces must also act on the sphere. •These forces trap the object and can be used to move it (like a tractor beam, just smaller ☺) •These tweezers can move objects as small as an atom This is a dual beam optical trap – these generate more force than a single beam, but also are a lot mor ...
... and opposite forces must also act on the sphere. •These forces trap the object and can be used to move it (like a tractor beam, just smaller ☺) •These tweezers can move objects as small as an atom This is a dual beam optical trap – these generate more force than a single beam, but also are a lot mor ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.