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Optoniks
Optoniks

... OPTONIKS has completed a 3D profilometry system prototype for Intel metrology lab which is responsible for measurement requirements and requests from other departments within Intel and also develops and assesses new metrology systems at Intel. Optonik’s next step is to launch development of more 3D ...
Frequency-Domain Spectroscopy Using 280 nm and 300 nm
Frequency-Domain Spectroscopy Using 280 nm and 300 nm

... the excitation light is modulated sinusoidally and the phase shift of the fluorescence, relative to the excitation, is determined). These methods have been reviewed numerous times (see, for example, [1-4]). The frequency domain approach has also been utilized with high-repetition rate pulsed sources ...
www.slac.stanford.edu
www.slac.stanford.edu

... The central point of the beam seen through an aperture looking ar the synchrotron light is the emission point, As the orbit moves transverselv in a bending maznet (due to orbit distortions, etc. ), the-emission point moves ‘axially. 2 One may calculate the angle of the line of emission points with r ...
Medical Laboratory Instrumentation 2010-2011 Third Year
Medical Laboratory Instrumentation 2010-2011 Third Year

... • 1. The source must emit radiation over the entire wavelength range to be studied. • 2. The intensity of radiation over the entire wavelength range must be high enough • so that extensive amplification of the signal from the detector can be avoided. • 3. The intensity of the source should not vary ...
Refraction - Snell`s Law, Internal Reflection, Dispersion (PowerPoint)
Refraction - Snell`s Law, Internal Reflection, Dispersion (PowerPoint)

... The fact that rainbows are semicircular tells you that the light from one has to come from the water drops at the right angle angle*. When white light enters a water drop light is refracted and disperses into its component colors. Violet is bent the most and red the least. When the rays reach the op ...
PH4027 - Optoelectronics & Nonlinear Optics
PH4027 - Optoelectronics & Nonlinear Optics

... a large number of everyday applications, ranging from the ubiquitous LED, televisions and computer displays, as well as lasers in CD players, to sophisticated equipment for high-speed telecommunications applications that form the backbone of the internet. While classical optics is concerned with lin ...
1 Introduction 2 Theory of Optical Trapping
1 Introduction 2 Theory of Optical Trapping

... In this section, a number of procedures for aligning and operating the setup are given. Please read them carefully. More detailed instructions will be given on the first day of the practical. • Never touch a mirror, lens, or filter with your fingers, since the fatty acids on your skin may degrade th ...
Ch. 35: Reflection and Refraction of Light
Ch. 35: Reflection and Refraction of Light

... • The wave properties of light show up in phenomena such as interference and diffraction. ...
Ch. 35: Reflection and Refraction of Light
Ch. 35: Reflection and Refraction of Light

... Light propagates in straight lines (rays). This is valid as long as the light does not change the medium through which it propagates (air, water, glass, plastic), or finds an obstacle (interface). The velocity of light in air is c c = 3x108 m/s The velocity of light in other media may be different f ...
Total Internal Reflection Fluorescence
Total Internal Reflection Fluorescence

... glass surface or close to the surface should be visible. The penetration depth of the evanescent field is dependent on the light’s angle of incidence, or conversely on how far the focus beam is away from the center of the objective’s BFP. If no FluoSpheres beads are sticking to the glass, a little b ...
Physical Optics - Haverford College
Physical Optics - Haverford College

... (with m as an integer), but one can also determine the relative intensities I() of the diffracted spots. If we insert a lens behind the diffraction grating, the effect of the lens serves to refocus the interference pattern so it reforms into an image of the diffraction grating. We will see shortly ...
supplementary info
supplementary info

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focusing of light by corneal lenses in a reflecting superposition eye
focusing of light by corneal lenses in a reflecting superposition eye

... In the reflecting superposition eyes described by Vogt and Land, parallel rays incident on an ommatidium are not focused by that ommatidium (except perhaps for the central ommatidium which tends to concentrate the light because of its taper), but are reflected by the multilayer mirrors around the cr ...
The Principle of Linear Superposition The Principle of Linear
The Principle of Linear Superposition The Principle of Linear

... opposite to the slit would be illuminated. • Instead, Huygens’ principle requires that the wave spreads out. In other words, the light deviates from a straight line path and enters a region that would otherwise be shadowed. • The divergence of light from its initial line of travel is called diffract ...
All students are asked for bringing your own samples which
All students are asked for bringing your own samples which

... Depth of field characterizes the extent to which image resolution degrades with distance above or below the plane of best focus. With greater depth of field a microscope can better image three dimensional samples. Although the SEM is best known for its excellent resolution, some of the most dramatic ...
File - Mrs. Hille`s FunZone
File - Mrs. Hille`s FunZone

... refracted. • All the light is reflected back into the denser material. • When this occurs it is called total internal reflection. ...
METHODS FOR DETERMINING BIOGENICITY IN ARCHEAN AND
METHODS FOR DETERMINING BIOGENICITY IN ARCHEAN AND

... PPRG). The syngenetic inclusions are either imbedded in the rock (and exposed by etching) or broken by fracturing the rock. The geochemistry reflects that of the groundmass, (i.e. calcium carbonate), usually with an elevated carbon content, which is a good signature for biogenicity. The Warrawoona c ...
Single Slit Diffraction & Gratings
Single Slit Diffraction & Gratings

... All the waves that originate at the slit are in phase  Wave 1 travels farther than wave 3 by an amount equal to the path difference (w/2)sinθ  If this path difference is exactly half of a wavelength, the two waves cancel each other and destructive interference results  In general, destructive int ...
In the diagram below, the optical train of a set of binoculars is found
In the diagram below, the optical train of a set of binoculars is found

... The Fraunhofer diffraction pattern from two microscopic circular holes separated by a distance d is observed at a wavelength of 500 nm on a screen placed 50 cm from the aperture plane. Inspection of the pattern shows that it is at the Rayleigh limit of resolution (i.e. the principal maximum appears ...
Shaping up LED Chips
Shaping up LED Chips

... and thus chip count across a wafer, might be compromised. Therefore, we propose the use of triangles and hexagons; such shapes can be closed-packed into any array without sacrificing chip space and thus make economic sense. An additional feature introduced into our optical setup enables machining of ...
OOSpecActivities
OOSpecActivities

... Introduction: Computer monitors are capable of producing millions of colors. However, close examination with a magnifying lens and the OO spec yields what may be some surprises. Only red, green, and blue pixels can be seen on a monitor. The computer adjusts the relative brightness of each color to p ...
Nonconfocal Differential Interferometry Sensing Scheme for
Nonconfocal Differential Interferometry Sensing Scheme for

... application of instruments for various samples to investigate the topography of specimens and the magnetic patterns. Previous efforts have confirmed that the use of a differential optical interferometer in scanning force microscope systems provides better DC stability and less optical path perturbation ...
Chapter 34 – Geometric Optics and Optical Instruments
Chapter 34 – Geometric Optics and Optical Instruments

... A glass rod 40 cm long and index 1.50 has one end that is convex of radius 20 cm and the other end has a convex surface of radius 30 cm. An object is placed 100 cm in front of the 20 cm convex surface. Where, what size, and what orientation is the final image? ...
Factors controlling heat exchange between the human body and its
Factors controlling heat exchange between the human body and its

... the clear reduced and magnified images of an object in the Bessel arrangement. Fix the lens in the positions relating to these images formations and, by moving the object through systematic (e.g. ±0,5cm) distances, find (and note) the object distances at which you obtain rather well recognizable ima ...
Polarization of Light Mica Sheet
Polarization of Light Mica Sheet

... digital watches to see what happens when the filter is placed in front of the display and rotated. Then ask them what filters actually do – let certain stuff through, while blocking other stuff (light in this case). So then ask what then a polarizing filter does, and they will say let through polari ...
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Microscopy



Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The development of microscopy revolutionized biology and remains an essential technique in the life and physical sciences.
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