Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... for antiviral chemotherapy. By analogy, this is almost certainly true for the 3C proteins of all picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein ex ...
... for antiviral chemotherapy. By analogy, this is almost certainly true for the 3C proteins of all picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein ex ...
Regulation of Cytochrome bd Expression in Mycobacterium
... Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable length. The cell wall in Mycobacteria is characteristic because it is thicker tha ...
... Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable length. The cell wall in Mycobacteria is characteristic because it is thicker tha ...
Ageing and the aggregating proteoglycans of
... appears to be at the N-terminus of the protein, and the smallest of the link proteins appears to be derived from either of the larger by proteolytic cleavage within this region [42]. Irrespective of the heterogeneity, all the link proteins are able to stabilize the proteoglycan aggregate towards dis ...
... appears to be at the N-terminus of the protein, and the smallest of the link proteins appears to be derived from either of the larger by proteolytic cleavage within this region [42]. Irrespective of the heterogeneity, all the link proteins are able to stabilize the proteoglycan aggregate towards dis ...
Nucleic Acids Research
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
Prediction of Folding, Stability and Structure of Proteins from Amino
... grouped in successive heptads of coiled-coil sequences found in the Swiss-Prot protein database. The hydrophobic residues appear in the ‘a’ and ‘d‘ heptad position in coiledcoil conformations. It has been proposed that clusters of hydrophobic amino acids in the ‘a’ and ‘d’ positions play an importan ...
... grouped in successive heptads of coiled-coil sequences found in the Swiss-Prot protein database. The hydrophobic residues appear in the ‘a’ and ‘d‘ heptad position in coiledcoil conformations. It has been proposed that clusters of hydrophobic amino acids in the ‘a’ and ‘d’ positions play an importan ...
Project 3: Visualizing Three-Dimensional Protein Structures Using
... the crystallographer who determined that structure? Most scientists who determine macromolecular structures are highly motivated to publish their findings in journals such as Science, Nature, Journal of Biological Chemistry, Journal of Molecular Biology and Protein Science. These journals have an ag ...
... the crystallographer who determined that structure? Most scientists who determine macromolecular structures are highly motivated to publish their findings in journals such as Science, Nature, Journal of Biological Chemistry, Journal of Molecular Biology and Protein Science. These journals have an ag ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
Structural Mechanisms for Regulation of Membrane
... Subfamily members tend to be functionally similar if not redundant (although differences in tissue and possibly subcellular distribution might be important), whereas group members typically have partially or completely distinguishable functions. Nevertheless, group members are more likely to functio ...
... Subfamily members tend to be functionally similar if not redundant (although differences in tissue and possibly subcellular distribution might be important), whereas group members typically have partially or completely distinguishable functions. Nevertheless, group members are more likely to functio ...
Purification and Properties of the Dictyostelium Calpain
... proteases that function as signal transduction components in a variety of important cellular pathways including sporulation and alkaline adaptation in fungi (1, 2), aleurone cell layer development in plant seeds (3), development of optic lobes in Drosophila melanogaster (4), and sex determination in ...
... proteases that function as signal transduction components in a variety of important cellular pathways including sporulation and alkaline adaptation in fungi (1, 2), aleurone cell layer development in plant seeds (3), development of optic lobes in Drosophila melanogaster (4), and sex determination in ...
CD-HIT User`s Guide - Bioinformatics.org
... Since its release, CD-‐HIT has been getting more and more popular. It has a significant user base, I estimated at over several thousands users. It is used at many research and educational ...
... Since its release, CD-‐HIT has been getting more and more popular. It has a significant user base, I estimated at over several thousands users. It is used at many research and educational ...
Molecular cloning, characterization and gene expression of an
... catalase-peroxidases and mono-functional catalases. The monofunctional catalases are the best characterized, and they are homo tetrameric and heme-containing enzymes. As catalases are found in organisms from eubacteria to eukaryotes [9,20e26], they are essential, strongly expressed, and tightly regu ...
... catalase-peroxidases and mono-functional catalases. The monofunctional catalases are the best characterized, and they are homo tetrameric and heme-containing enzymes. As catalases are found in organisms from eubacteria to eukaryotes [9,20e26], they are essential, strongly expressed, and tightly regu ...
Fibrous Proteins
... (2) Amino acid composition of elastin 33% Gly 10% Pro and Hyp 23% Ala 13% Val Hence 79% of the residues come from 4 amino acids. There are large hydrophobic peptides rich in Ala, Val, Ile and Leu. As these sidechains do not interact with each other by hydrogen bonds, they enable the core of elastin ...
... (2) Amino acid composition of elastin 33% Gly 10% Pro and Hyp 23% Ala 13% Val Hence 79% of the residues come from 4 amino acids. There are large hydrophobic peptides rich in Ala, Val, Ile and Leu. As these sidechains do not interact with each other by hydrogen bonds, they enable the core of elastin ...
Read more... - Bonanza Calf Nutrition
... Feed labels contain information required by law and show the levels of CRUDE constituents contained in the product. Calves are infants and the ingredients used in their diet are milk ingredients not plant material. They cannot use Crude protein or convert it, like ruminants, into protein and calves ...
... Feed labels contain information required by law and show the levels of CRUDE constituents contained in the product. Calves are infants and the ingredients used in their diet are milk ingredients not plant material. They cannot use Crude protein or convert it, like ruminants, into protein and calves ...
Geometrical and Sequence Characteristics of
... 1992; Hobohm and Sander, 1994) to select a subset database of 205 nonhomologous globular protein chains whose three-dimensional structures have been solved by x-ray crystallography to a resolution of 2.5 Å or better. One thousand one hundred thirty-one (1131) a-helices of nonidentical sequences and ...
... 1992; Hobohm and Sander, 1994) to select a subset database of 205 nonhomologous globular protein chains whose three-dimensional structures have been solved by x-ray crystallography to a resolution of 2.5 Å or better. One thousand one hundred thirty-one (1131) a-helices of nonidentical sequences and ...
Cephalosporin chemical reactivity and its immunological
... reactivity of the two electrophilic centers of the molecule, with a reduction in the reactivity of the carbonyl group of the b-lactam ring and, consequently, the possible competence of the 30 position enabling the formation of conjugates with a form such as structure 5 (Fig. 8) [37]. In cephalospori ...
... reactivity of the two electrophilic centers of the molecule, with a reduction in the reactivity of the carbonyl group of the b-lactam ring and, consequently, the possible competence of the 30 position enabling the formation of conjugates with a form such as structure 5 (Fig. 8) [37]. In cephalospori ...
Prokaryotic features of a nucleus
... sequences and relatively similar to the GAPDHs of thermophilic bacteria. These results are compatible with the hypothesis that the nuclear gene for subunit A of chloroplast GAPDH is of prokaryotic origin. They are in puzzling contrast with a previous publication demonstrating that Escherichia coli G ...
... sequences and relatively similar to the GAPDHs of thermophilic bacteria. These results are compatible with the hypothesis that the nuclear gene for subunit A of chloroplast GAPDH is of prokaryotic origin. They are in puzzling contrast with a previous publication demonstrating that Escherichia coli G ...
REVIEWS Structural insights into the function of the
... plete loss of activity, and/or target the binding in many nucleotide-binding pro- COX, GOX and GR) can be generally misfolded protein for degradation. In teins 37. The tightly bound FAD in flavo- classified into three groups: (1) oxithe case of CHM/REP, these would lead proteins occupies a long groo ...
... plete loss of activity, and/or target the binding in many nucleotide-binding pro- COX, GOX and GR) can be generally misfolded protein for degradation. In teins 37. The tightly bound FAD in flavo- classified into three groups: (1) oxithe case of CHM/REP, these would lead proteins occupies a long groo ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... Homologs of the investigated proteins (RBCL, RBCS, RuBisCO activase RCA, and RuBisCO assembly chaperone RAF) were identified by BLAST [32] in the non-redundant protein database at NCBI, in transcriptome assemblies of relevant species generated by the Marine Microbial Eukaryote Transcriptome Sequenci ...
... Homologs of the investigated proteins (RBCL, RBCS, RuBisCO activase RCA, and RuBisCO assembly chaperone RAF) were identified by BLAST [32] in the non-redundant protein database at NCBI, in transcriptome assemblies of relevant species generated by the Marine Microbial Eukaryote Transcriptome Sequenci ...
Medicinal Chemistry Reviews
... suppressing tRNA with unnatural amino acids permitted sitespecific incorporation of virtually any amino acid, but it requires a substantial synthetic undertaking and resulted in poor yields of protein from in vitro translation mixtures. In order to achieve this same type of incorporation in living c ...
... suppressing tRNA with unnatural amino acids permitted sitespecific incorporation of virtually any amino acid, but it requires a substantial synthetic undertaking and resulted in poor yields of protein from in vitro translation mixtures. In order to achieve this same type of incorporation in living c ...
OUR PRODUCTS
... tissue growth and repair. In addition to protein, quinoa features a host of other healthbuilding nutrients. Because quinoa is a very good source of manganese as well as a good source of magnesium, iron, copper and phosphorous, this "grain" may be especially valuable for persons with migraine headach ...
... tissue growth and repair. In addition to protein, quinoa features a host of other healthbuilding nutrients. Because quinoa is a very good source of manganese as well as a good source of magnesium, iron, copper and phosphorous, this "grain" may be especially valuable for persons with migraine headach ...
Promiscuity of the Euonymus Carbohydrate-Binding Domain
... might contribute towards dehydration tolerance [27]. Furthermore Moons et al. [20] suggested that some rice EUL proteins play an important role in the response of plant tissues to salt and osmotic stress. An in silico expression analysis for the EUL from Arabidopsis demonstrated that this lectin gen ...
... might contribute towards dehydration tolerance [27]. Furthermore Moons et al. [20] suggested that some rice EUL proteins play an important role in the response of plant tissues to salt and osmotic stress. An in silico expression analysis for the EUL from Arabidopsis demonstrated that this lectin gen ...
Jelly Facts - Institute of Food Research
... gelatin needs to be about 1% to form jelly The strength of a gelatin-based gel is its bloom strength and can be measured with a penetrometer or gelometer. ...
... gelatin needs to be about 1% to form jelly The strength of a gelatin-based gel is its bloom strength and can be measured with a penetrometer or gelometer. ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... designed. Therefore, much effort has gone into creating programs for primer design. PCR is used for a variety of applications depending upon the question of biological interest. For instance, variations on the PCR protocol include multiplex PCR for multiple genes, bisulfite PCR for methylated DNA, a ...
... designed. Therefore, much effort has gone into creating programs for primer design. PCR is used for a variety of applications depending upon the question of biological interest. For instance, variations on the PCR protocol include multiplex PCR for multiple genes, bisulfite PCR for methylated DNA, a ...
Mechanistic Studies Of Drug Resistance Conferred By An ABC
... work could never be done. I am grateful for her well-rounded guidance and support throughout all these years’ Ph.D studies. Her understanding, sincerity and patience have encouraged me to overcome the obstacles and challenges that I encountered during my research. I could not remember how many times ...
... work could never be done. I am grateful for her well-rounded guidance and support throughout all these years’ Ph.D studies. Her understanding, sincerity and patience have encouraged me to overcome the obstacles and challenges that I encountered during my research. I could not remember how many times ...
Homology modeling
Homology modeling, also known as comparative modeling of protein, refers to constructing an atomic-resolution model of the ""target"" protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein (the ""template""). Homology modeling relies on the identification of one or more known protein structures likely to resemble the structure of the query sequence, and on the production of an alignment that maps residues in the query sequence to residues in the template sequence. It has been shown that protein structures are more conserved than protein sequences amongst homologues, but sequences falling below a 20% sequence identity can have very different structure.Evolutionarily related proteins have similar sequences and naturally occurring homologous proteins have similar protein structure.It has been shown that three-dimensional protein structure is evolutionarily more conserved than would be expected on the basis of sequence conservation alone.The sequence alignment and template structure are then used to produce a structural model of the target. Because protein structures are more conserved than DNA sequences, detectable levels of sequence similarity usually imply significant structural similarity.The quality of the homology model is dependent on the quality of the sequence alignment and template structure. The approach can be complicated by the presence of alignment gaps (commonly called indels) that indicate a structural region present in the target but not in the template, and by structure gaps in the template that arise from poor resolution in the experimental procedure (usually X-ray crystallography) used to solve the structure. Model quality declines with decreasing sequence identity; a typical model has ~1–2 Å root mean square deviation between the matched Cα atoms at 70% sequence identity but only 2–4 Å agreement at 25% sequence identity. However, the errors are significantly higher in the loop regions, where the amino acid sequences of the target and template proteins may be completely different.Regions of the model that were constructed without a template, usually by loop modeling, are generally much less accurate than the rest of the model. Errors in side chain packing and position also increase with decreasing identity, and variations in these packing configurations have been suggested as a major reason for poor model quality at low identity. Taken together, these various atomic-position errors are significant and impede the use of homology models for purposes that require atomic-resolution data, such as drug design and protein–protein interaction predictions; even the quaternary structure of a protein may be difficult to predict from homology models of its subunit(s). Nevertheless, homology models can be useful in reaching qualitative conclusions about the biochemistry of the query sequence, especially in formulating hypotheses about why certain residues are conserved, which may in turn lead to experiments to test those hypotheses. For example, the spatial arrangement of conserved residues may suggest whether a particular residue is conserved to stabilize the folding, to participate in binding some small molecule, or to foster association with another protein or nucleic acid. Homology modeling can produce high-quality structural models when the target and template are closely related, which has inspired the formation of a structural genomics consortium dedicated to the production of representative experimental structures for all classes of protein folds. The chief inaccuracies in homology modeling, which worsen with lower sequence identity, derive from errors in the initial sequence alignment and from improper template selection. Like other methods of structure prediction, current practice in homology modeling is assessed in a biennial large-scale experiment known as the Critical Assessment of Techniques for Protein Structure Prediction, or CASP.