Molecular Biology
... • The alphabet of RNA is A, U, G and C • Within a molecule of mRNA, groups of 3 sequential nucleotides form meaningful “words” called codons – complementary to triplets in the template strand of the gene that was transcribed by RNA polymerase • each codon is a code for an amino acid of the protein c ...
... • The alphabet of RNA is A, U, G and C • Within a molecule of mRNA, groups of 3 sequential nucleotides form meaningful “words” called codons – complementary to triplets in the template strand of the gene that was transcribed by RNA polymerase • each codon is a code for an amino acid of the protein c ...
EB nucleic acids AP
... A scientist extracts DNA from the nucleus of cells and sequences it. The scientist determines that 27% of the nucleotide bases are guanine. What percentage of the bases are thymine? ...
... A scientist extracts DNA from the nucleus of cells and sequences it. The scientist determines that 27% of the nucleotide bases are guanine. What percentage of the bases are thymine? ...
DNA Sequencing
... The single stranded DNA fragment to be sequenced is end-labeled by treatment with alkaline phosphatase to remove the 5’phosphate It is then followed by reaction with P-labeled ATP in the presence of polynucleotide kinase, which attaches P labeled to the 5’terminal The labeled DNA fragment is then ...
... The single stranded DNA fragment to be sequenced is end-labeled by treatment with alkaline phosphatase to remove the 5’phosphate It is then followed by reaction with P-labeled ATP in the presence of polynucleotide kinase, which attaches P labeled to the 5’terminal The labeled DNA fragment is then ...
unit 7 exam study guide
... 15. What makes up the “backbone” of a DNA molecule? 16. What makes up the "rungs" of a DNA molecule? 17. What type of bonds holds the DNA bases together? 18. Explain Chargaff’s discovery. 19. If a DNA molecule contains 22% adenine, what percentages of the other bases would be present? 20. If the seq ...
... 15. What makes up the “backbone” of a DNA molecule? 16. What makes up the "rungs" of a DNA molecule? 17. What type of bonds holds the DNA bases together? 18. Explain Chargaff’s discovery. 19. If a DNA molecule contains 22% adenine, what percentages of the other bases would be present? 20. If the seq ...
DNA is the genetic material DNA structure
... sequence of the bases • Complementary base pairing of strands that are NOT covalently bound: suggests mechanism for ...
... sequence of the bases • Complementary base pairing of strands that are NOT covalently bound: suggests mechanism for ...
As a group, quietly discuss each question and agree
... Part A: During DNA replication, which enzyme breaks the hydrogen bonds allowing the DNA to separate? ...
... Part A: During DNA replication, which enzyme breaks the hydrogen bonds allowing the DNA to separate? ...
File
... 11. On the diagram to the right, draw arrows to label the new and old strands of DNA. 12. Sequence the steps of DNA replication below from 1 - 6. a) _____ Free nucleotides pair with open nucleotides b) _____ An enzyme proofreads to check for replication errors c) _____ Weak hydrogen bonds are broken ...
... 11. On the diagram to the right, draw arrows to label the new and old strands of DNA. 12. Sequence the steps of DNA replication below from 1 - 6. a) _____ Free nucleotides pair with open nucleotides b) _____ An enzyme proofreads to check for replication errors c) _____ Weak hydrogen bonds are broken ...
mutation
... Ionizing radiation, in particular, causes breaks in both DNA strands. Also, several immune deficiency diseases and familial breast and ovarian cancers may be a result of double strand DNA breaks. ...
... Ionizing radiation, in particular, causes breaks in both DNA strands. Also, several immune deficiency diseases and familial breast and ovarian cancers may be a result of double strand DNA breaks. ...
Gel Electrophoresis DNA Fingerprinting
... • In this hypothetical case, DNA was extracted from samples obtained from the five possible suspects, and the crime scene sample • You will cleave the DNA with a restriction enzyme and simulated a “mock” DNA fingerprint analysis using Southern Blotting ...
... • In this hypothetical case, DNA was extracted from samples obtained from the five possible suspects, and the crime scene sample • You will cleave the DNA with a restriction enzyme and simulated a “mock” DNA fingerprint analysis using Southern Blotting ...
DNA to Proteins
... bond to the separated DNA chains (A to T and G to C) • DNA polymerase enzymes bond new DNA nucleotides to the original DNA strands • The original strand serves as a template for the new strand ...
... bond to the separated DNA chains (A to T and G to C) • DNA polymerase enzymes bond new DNA nucleotides to the original DNA strands • The original strand serves as a template for the new strand ...
CHEM 331 Problem Set #7
... 4. One strand of a double-helical DNA has the sequence (5ʼ)GCGCAATATTTCTCAAAATATTGCGC(3ʼ) Write the base sequence of the complementary strand. What special type of sequence is contained in this DNA segment? Does the double-stranded DNA have the potential to form any alternative structures? (3 pts.) ...
... 4. One strand of a double-helical DNA has the sequence (5ʼ)GCGCAATATTTCTCAAAATATTGCGC(3ʼ) Write the base sequence of the complementary strand. What special type of sequence is contained in this DNA segment? Does the double-stranded DNA have the potential to form any alternative structures? (3 pts.) ...
Chap 3
... for proteins 3. The human genome has significantly more duplicated segments within it that other mammalian genomes do. These sections may be the source of new primatespecific genes. 4. At the time when the draft sequence was published less than 7% of protein families appeared to be vertebrate-specif ...
... for proteins 3. The human genome has significantly more duplicated segments within it that other mammalian genomes do. These sections may be the source of new primatespecific genes. 4. At the time when the draft sequence was published less than 7% of protein families appeared to be vertebrate-specif ...
Review sheet – Chapter 10
... Understand that DNA replication occurs on both strands, with the old (parental strand) serving as a template for the new (daughter) strand being laid down (synthesized), resulting in 2 complete DNA molecules, each consisting of a double helix of a parental and daughter strand ...
... Understand that DNA replication occurs on both strands, with the old (parental strand) serving as a template for the new (daughter) strand being laid down (synthesized), resulting in 2 complete DNA molecules, each consisting of a double helix of a parental and daughter strand ...
DNA consists of two strands, each of which is a linear arrangement
... mechanism for replication. If the double helix begins to unwind and the two strands separate, free nucleotides present in the cell are able to pair with the bases of each strand, forming a new and complementary strand for each of the original strands. As the unwinding proceeds, two double helixes ar ...
... mechanism for replication. If the double helix begins to unwind and the two strands separate, free nucleotides present in the cell are able to pair with the bases of each strand, forming a new and complementary strand for each of the original strands. As the unwinding proceeds, two double helixes ar ...
MCB 110 Problem set 2. DNA replication - Answers
... oversimplification in the figure. In eukaryotes, the replicative helicase is made up of six different homologous subunits (as shown), and there is a hand-off of the lagging strand from primase to pol α to pol δ. 11. What are two roles for topoisomerases in DNA replication? Could a type 1 topoisomera ...
... oversimplification in the figure. In eukaryotes, the replicative helicase is made up of six different homologous subunits (as shown), and there is a hand-off of the lagging strand from primase to pol α to pol δ. 11. What are two roles for topoisomerases in DNA replication? Could a type 1 topoisomera ...
Valhalla High School
... Since DNA is too large of a molecule to fit outside the nucleus, a messenger is needed to get to the ribosome. DNA is converted into a single stranded RNA molecule, called mRNA. This process is called transcription. Draw your codon lines to separate the triplets. Using the base pairing rules for DNA ...
... Since DNA is too large of a molecule to fit outside the nucleus, a messenger is needed to get to the ribosome. DNA is converted into a single stranded RNA molecule, called mRNA. This process is called transcription. Draw your codon lines to separate the triplets. Using the base pairing rules for DNA ...
PCR: Basics & Miniturization
... rate leads to long lines for the machine and sign up sheets that force you to start your reactions at all hours. The RapidCycler™ can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry. No more waiting in line. You can ...
... rate leads to long lines for the machine and sign up sheets that force you to start your reactions at all hours. The RapidCycler™ can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry. No more waiting in line. You can ...
Palindromic Sequences
... Insulin is a dimer, composed of one A chain (fraction A) and a B chain (fraction B). Sanger and Tuppy determined this experimentally by oxidizing insulin with performic acid. The sequence of fraction B was determined experimentally as being at least composed of phenylalanine, valine, aspartic acid, ...
... Insulin is a dimer, composed of one A chain (fraction A) and a B chain (fraction B). Sanger and Tuppy determined this experimentally by oxidizing insulin with performic acid. The sequence of fraction B was determined experimentally as being at least composed of phenylalanine, valine, aspartic acid, ...
Restriction Enzymes - Seattle Central College
... • Restriction enzymes are bacterial enzymes that cleave the sugar-phosphate backbone of the DNA at specific nucleotide. • They are member of the class of nucleases. Endonucleases cleave nucleic acid at internal positions, while exonucleases progressively digest from the ends of the nucleic acid mole ...
... • Restriction enzymes are bacterial enzymes that cleave the sugar-phosphate backbone of the DNA at specific nucleotide. • They are member of the class of nucleases. Endonucleases cleave nucleic acid at internal positions, while exonucleases progressively digest from the ends of the nucleic acid mole ...
chapter 12 practice test - open to see diagrams
... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. 3. Unlike DNA, RNA contains a. adenine. c. phosphate groups. b. uracil. d. thymine. 4. Which type(s) of RNA is(ar ...
... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. 3. Unlike DNA, RNA contains a. adenine. c. phosphate groups. b. uracil. d. thymine. 4. Which type(s) of RNA is(ar ...
The Structure of DNA and RNA
... DNA is a double helix formed by base pairs attached to a sugarphosphate backbone. ...
... DNA is a double helix formed by base pairs attached to a sugarphosphate backbone. ...
BIOCHEMISTRY Nucleic Acids
... present in the nucleus (C≡G & A=T) one at a time & form new hydrogen bonds with the old strand (= the template). • The enzyme DNA-polymerase checks if the pairing of bases is correct & joints the new bases to a new backbone (catalyzes formation of new phosphodiester bonds between nucleotides). • Eac ...
... present in the nucleus (C≡G & A=T) one at a time & form new hydrogen bonds with the old strand (= the template). • The enzyme DNA-polymerase checks if the pairing of bases is correct & joints the new bases to a new backbone (catalyzes formation of new phosphodiester bonds between nucleotides). • Eac ...
Molecular structure of nucleic acids. A Structure for Deoxyribose
... has novel features which are of considerable biological interest. A structure for nucleic acid has already been proposed by Pauling and Corey [1]. Their model consists of three intertwined chains, with the phosphates near the fibre axis, and the bases on the outside. We wish to put forward a radical ...
... has novel features which are of considerable biological interest. A structure for nucleic acid has already been proposed by Pauling and Corey [1]. Their model consists of three intertwined chains, with the phosphates near the fibre axis, and the bases on the outside. We wish to put forward a radical ...
Polymerase Chain Reaction and PTC lab
... 1. Denature DNA by heating to 95oC to separate strands 2. Anneal primers by cooling to 37oC so primers bond to DNA strands 3. Extend DNA strand by heating to 72oC so Taq can build the complementary strand Repeat over and over until you have the desired amount of DNA Gel Electrophoresis One indi ...
... 1. Denature DNA by heating to 95oC to separate strands 2. Anneal primers by cooling to 37oC so primers bond to DNA strands 3. Extend DNA strand by heating to 72oC so Taq can build the complementary strand Repeat over and over until you have the desired amount of DNA Gel Electrophoresis One indi ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.