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Nükleik Asitler - mustafaaltinisik.org.uk
Nükleik Asitler - mustafaaltinisik.org.uk

... • Supercoiling prevalent in circular DNA molecules and within local regions of long linear DNA strands • Enzymes called topoisomerases or gyrases can introduce or remove supercoils • In vivo most DNA is negatively supercoiled. • Therefore, it is easy to unwind short regions of the molecule to allow ...
Chapter 9
Chapter 9

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Lecture 2 Nucleic Acid Structure

... -Melting duplex to singlestranded is accompanied by an increase in absorbance -Tm depends on length, ionic strength and GC/AT ratio Tm calculator: ...
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... sequence homology, as in two homologous chromosomes, for example. This is in contrast to site‐specific  recombination  (to  be  discussed  later),  in  which  DNA  exchange  occurs  within  well‐defined  short  DNA  segments. The extent of actual strand exchange during site‐specific recombination do ...
Applied Biology DNA structure & replication
Applied Biology DNA structure & replication

... Base-pairing rule- each base must pair up with its complementary base. ...
Christ The King School Exampro A-level Biology (7401/7402) DNA
Christ The King School Exampro A-level Biology (7401/7402) DNA

... Give two ways in which the structure of a molecule of tRNA differs from the structure of a molecule of mRNA. ...
5 Conclusion - Duke Computer Science
5 Conclusion - Duke Computer Science

... molecule is attached to the connecting helix. We have used the B-Z transition as the basis for the structural alteration we wish to induce. It changes shape predicated on this B-Z transition. We detected relative strand position changes by fluorescence resonance energy transfer(FRET), because the se ...
Structure and function of DNA
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... DNA is a double stranded molecule consists of 2 polynucleotide chains running in opposite directions. Both strands are complementary to each other. The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. Th ...
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Structure and function of DNA
Structure and function of DNA

... DNA is a double stranded molecule consists of 2 polynucleotide chains running in opposite directions. Both strands are complementary to each other. The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. Th ...
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DNA - Snow Elementary School
DNA - Snow Elementary School

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ppt link
ppt link

... Dispersive replication: At completion, both strands of both double helices contain both original and newly synthesized material. ...
No Slide Title
No Slide Title

... 1. Heat the mixture. Just before the boiling point of water, the DNA will become single-stranded. 2. Cool the mixture. As the mixture cools, the primer will bind to the DNA and the polymerase will synthesize a new strand for each strand of DNA. 3. Repeat steps 1 and 2 until a sufficient amount of th ...
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Model question Paper- Gene Technology MLAB 475
Model question Paper- Gene Technology MLAB 475

... C) A gene is cloned in the MCS of lacZ’ of E. coli. The transformants are selected by blue white selection- lacZ’ complementation on X-gal plate and two kinds of colonies are observed. Explain the significance of blue and white ...
Replication, Transcription, Translation
Replication, Transcription, Translation

... Transcription is the synthesis of rRNA, tRNA, and mRNA, using the nucleotide sequence information from DNA. The RNA is synthesized as a complementary copy of one of the two DNA single strands (template strand), in a process similar to DNA synthesis. The template strand in a transcription bubble is r ...
DNA Structure and Sequencing - SP14
DNA Structure and Sequencing - SP14

... The size of the genome in one of the most well-studied prokaryotes, E.coli, is 4.6 million base pairs (approximately 1.1 mm, if cut and stretched out). So how does this t inside a small bacterial cell? The DNA is twisted by what is known as supercoiling. Supercoiling means that DNA is either under- ...
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Study Guide: Meiosis and Genetics

... 10.2.3 Explain how crossing over of non-sister chromatids of a homologous pair in Prophase 1 can result in an exchange of alleles. ...
TUTORIAL FIGURES: Basic Molecular Biology
TUTORIAL FIGURES: Basic Molecular Biology

... units on a chain are held together by phosphodiester bonds and the two chains are held together by hydrogen bonds (· · · ) between A-T and G-C bases exclusively. Chemical structures of the bases are such that A pairs with T and G pairs with C only (base pairing rules). Bases of RNA (ribonucleic acid ...
Ch. 11 - Holden R-III School District
Ch. 11 - Holden R-III School District

... Evolutionary relationships, whether or not 2 people are related, and identity confirmation of crime victims can all be determined by analyzing this information ...
DNA Replication - inetTeacher.com
DNA Replication - inetTeacher.com

... DNA must be carried to a place where there are materials for the proteins to be created. Where are proteins created? DNA is copied to mRNA because DNA is too big to leave through the nuclear pores. If DNA was not copied the code could not be transferred to the place it can be created. ...
summary - VU Research Portal
summary - VU Research Portal

... through clear patterns. Eventually, this led to the founding of a new field: molecular biology. A milestone in this process is the discovery of the structure of DNA by Watson and Crick in 1953. The next decades more and more details on life at a microscopic level were revealed. The flow of informati ...
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Holliday junction



A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.
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