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Ribonucleic acids are found in both the nucleus and the cytoplasm
Ribonucleic acids are found in both the nucleus and the cytoplasm

... genes into the amino acid sequences of proteins. The three universal types of RNA include transfer RNA (tRNA), messenger RNA (mRNA), and ribosomal RNA (rRNA). Messenger RNA acts to carry genetic sequence information between DNA and ribosomes, directing protein synthesis. Ribosomal RNA is a major com ...
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... use PCR to 1) amplify a genetic locus to detect a genomic polymorphism and 2) to make a site-specific mutation in a plasmid DNA  Isolate yeast genomic DNA from a haploid mutant (sqs1::KAN), a haploid wildtype (SQS1) and heterozygous mutant/wildtype diploid strain (sqs1::KAN/SQS1  Conduct standard ...
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Holliday junction



A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.
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