Discovery of a novel ligand that modulates the
... through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtained through chemical optimization (see Scheme S1 and Fig. S4 and S5†). (b) The Autodock prediction of the binding mode of 1 is shown as black st ...
... through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtained through chemical optimization (see Scheme S1 and Fig. S4 and S5†). (b) The Autodock prediction of the binding mode of 1 is shown as black st ...
GYP5 - Genetics
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
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... and the plasma membrane. Here, we report the identification of COD1/SPF1 (control of HMGCoA reductase degradation/SPF1) through genetic strategies intended to uncover genes involved in protein maturation and endoplasmic reticulum (ER)-associated degradation (ERAD), a quality control pathway that rid ...
... and the plasma membrane. Here, we report the identification of COD1/SPF1 (control of HMGCoA reductase degradation/SPF1) through genetic strategies intended to uncover genes involved in protein maturation and endoplasmic reticulum (ER)-associated degradation (ERAD), a quality control pathway that rid ...
Pavel Doležal
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
Pavel Doležal
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
Replacing Traditional SDS-PAGE and Bradford Techniques
... Each fraction was analyzed on the 5100 ALP using the Protein 200 HT-2 assay. The principle of analysis used with the 5100 ALP is identical to that used with the 2100 bioanalyzer [3, 4]. Proteins are denatured with sample buffer containing dodecylsulfate and two internal standards (lower and upper ma ...
... Each fraction was analyzed on the 5100 ALP using the Protein 200 HT-2 assay. The principle of analysis used with the 5100 ALP is identical to that used with the 2100 bioanalyzer [3, 4]. Proteins are denatured with sample buffer containing dodecylsulfate and two internal standards (lower and upper ma ...
P1 and P2 protein heterodimer binding to the P0 protein of
... washing protocol were estimated in immunoblots probed with specific monoclonal antibodies against proteins P0, P1b, P2a and P2b. The ratio of each protein to P0 was comparable in the ribosomes of each mutant strain and the parental W303 strain (Figure 5). Although an equivalent monoclonal antibody to ...
... washing protocol were estimated in immunoblots probed with specific monoclonal antibodies against proteins P0, P1b, P2a and P2b. The ratio of each protein to P0 was comparable in the ribosomes of each mutant strain and the parental W303 strain (Figure 5). Although an equivalent monoclonal antibody to ...
protease (NS34A) and an RNA polymerase
... rapid degradation6. However, if mitochondria are defective or damaged (for example by exposure to CCCP, a poison that blocks ATP production), PINK1 accumulates, becoming anchored to the outer mitochondrial mem brane with its kinase domain exposed to the cytoplasm. Damaged mitochondria also attract ...
... rapid degradation6. However, if mitochondria are defective or damaged (for example by exposure to CCCP, a poison that blocks ATP production), PINK1 accumulates, becoming anchored to the outer mitochondrial mem brane with its kinase domain exposed to the cytoplasm. Damaged mitochondria also attract ...
The Roles of Moonlighting Proteins in Bacteria
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
Proteins: Fundamental Chemical Properties
... quantitatively and qualitatively, which accounts for the origin of their name derived from the Greek word prôtos, meaning ‘first rank of importance’. Quantitatively, proteins are the most abundant class of biomolecules since they represent over 50% of the dry weight of cells, far more than other imp ...
... quantitatively and qualitatively, which accounts for the origin of their name derived from the Greek word prôtos, meaning ‘first rank of importance’. Quantitatively, proteins are the most abundant class of biomolecules since they represent over 50% of the dry weight of cells, far more than other imp ...
UvA-DARE (Digital Academic Repository) Mitochondria in neutrophil
... family member Bfl-1. Expression of mRNA for this protein was enhanced by G-CSF and very high in resting neutrophils, while the expression remained stable during apoptosis. However, at the protein level, Bfl-1 was only expressed during the later stages of spontaneous apoptosis in a fraction that cont ...
... family member Bfl-1. Expression of mRNA for this protein was enhanced by G-CSF and very high in resting neutrophils, while the expression remained stable during apoptosis. However, at the protein level, Bfl-1 was only expressed during the later stages of spontaneous apoptosis in a fraction that cont ...
1 Evolutionary conservation and emerging functional diversity of the
... sick phenotype of ydj1Δ like full-length Ydj1 (Figure 2A, Figure S1). Next, we began to investigate the functionality of other more specialized J protein orthologs. Jjj1 is a class III J protein that associates with the ribosome and plays an important and unique role in the biogenesis of the 60S ri ...
... sick phenotype of ydj1Δ like full-length Ydj1 (Figure 2A, Figure S1). Next, we began to investigate the functionality of other more specialized J protein orthologs. Jjj1 is a class III J protein that associates with the ribosome and plays an important and unique role in the biogenesis of the 60S ri ...
Structural and enzymatic characterization of a glycoside hydrolase
... cellulosome complexes [7], but are instead either secreted into the medium or attached to the cell membrane as lipoproteins. Indeed, approximately one-third of the encoded carbohydrate-degrading enzymes have been predicted to be lipoproteins [6], which may suggest that C. japonicus has developed a s ...
... cellulosome complexes [7], but are instead either secreted into the medium or attached to the cell membrane as lipoproteins. Indeed, approximately one-third of the encoded carbohydrate-degrading enzymes have been predicted to be lipoproteins [6], which may suggest that C. japonicus has developed a s ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... ftsZ genes belong to two distinct families: ftsZ1 and ftsZ2. However, the roles of the corresponding proteins FtsZ1 and FtsZ2 in plastid division have not been determined. Here we show that the expression of plant FtsZ1 and FtsZ2 in bacteria has different effects on cell division, and that distinct ...
... ftsZ genes belong to two distinct families: ftsZ1 and ftsZ2. However, the roles of the corresponding proteins FtsZ1 and FtsZ2 in plastid division have not been determined. Here we show that the expression of plant FtsZ1 and FtsZ2 in bacteria has different effects on cell division, and that distinct ...
Transport of proteins across mitochondrial membranes
... folding of proteins during transmembrane transfer are closely linked to the function of a host of proteins known as heat-shock proteins or molecular chaperones, present both outside and inside the mitochondrion. In addition, the analysis of the process of folding of polypeptides in the mitochondrial ...
... folding of proteins during transmembrane transfer are closely linked to the function of a host of proteins known as heat-shock proteins or molecular chaperones, present both outside and inside the mitochondrion. In addition, the analysis of the process of folding of polypeptides in the mitochondrial ...
Targeting of interleukin-2 to the periplasm of
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
Do Legume Storage Proteins Play a Role in
... et al., 1995; Shutov et al., 1995). Three-dimensional structures of several of these proteins have been solved by x-ray crystallography confirming the symmetry of the molecule and showing the arrangement of the subunits in the trimer (Ng et al., 1993). There is also sequence identity between legumin ...
... et al., 1995; Shutov et al., 1995). Three-dimensional structures of several of these proteins have been solved by x-ray crystallography confirming the symmetry of the molecule and showing the arrangement of the subunits in the trimer (Ng et al., 1993). There is also sequence identity between legumin ...
Amyloid Deposits: Protection Against Toxic Protein
... who had exhibited progressive cognitive dysfunction and psychosis1. It is now known that the majority of neurodegenerative diseases characterized by progressive neuronal dysfunction and loss are associated with the deposition of misfolded proteins. These misfolded proteins are frequently found in a ...
... who had exhibited progressive cognitive dysfunction and psychosis1. It is now known that the majority of neurodegenerative diseases characterized by progressive neuronal dysfunction and loss are associated with the deposition of misfolded proteins. These misfolded proteins are frequently found in a ...
Structure and assembly of the spliceosomal small nuclear
... used to investigate pairwise interactions of the Sm proteins [33•,34•]. Kambach et al. [32••] have been able to arrange all seven Sm proteins within a seven-membered ring (Figure 1d) in a manner that is consistent with all the known pairwise interactions [33•,34•]. The heptameric ring is the only co ...
... used to investigate pairwise interactions of the Sm proteins [33•,34•]. Kambach et al. [32••] have been able to arrange all seven Sm proteins within a seven-membered ring (Figure 1d) in a manner that is consistent with all the known pairwise interactions [33•,34•]. The heptameric ring is the only co ...
Releasable conjugation of polymers to proteins
... currently exist residue-specific reactions for permanently coupling polymers to at least 10 out of the 20 canonical amino acids found in proteins.7-10 However, in many cases, polymer-modification can lead to significant (or total) loss of peptide/protein activity. A classic example is lysozyme, whos ...
... currently exist residue-specific reactions for permanently coupling polymers to at least 10 out of the 20 canonical amino acids found in proteins.7-10 However, in many cases, polymer-modification can lead to significant (or total) loss of peptide/protein activity. A classic example is lysozyme, whos ...
Short review - BioPublisher
... independent organelle structures such as mitochondria and chloroplasts. These subcellular structures include nucleus, endoplasmic reticulum (ER), Golgi apparatus, lysosome, peroxisome, vacuole, cytoskeleton, cytosol, mitochondrion, chloroplast, and plasma membrane. For a subcellular membrane enclose ...
... independent organelle structures such as mitochondria and chloroplasts. These subcellular structures include nucleus, endoplasmic reticulum (ER), Golgi apparatus, lysosome, peroxisome, vacuole, cytoskeleton, cytosol, mitochondrion, chloroplast, and plasma membrane. For a subcellular membrane enclose ...
Protein translocation pathways across the inner and outer
... membrane proteins with α-helical transmembrane segment e.g. Tom20 can be inserted into outer mitochondrial membrane with help of TOM complex alone27. The two additional subunits of SAMcore complex are Sam50/Tob55 and Sam35/Tob38. Sam50 assembles as an integral outer membrane protein with a predicted ...
... membrane proteins with α-helical transmembrane segment e.g. Tom20 can be inserted into outer mitochondrial membrane with help of TOM complex alone27. The two additional subunits of SAMcore complex are Sam50/Tob55 and Sam35/Tob38. Sam50 assembles as an integral outer membrane protein with a predicted ...
Chapter 14-Respiration
... Model for ATP synthesis (Paul Boyer 1979) 1. A molecule of ADP and one Pi bind to an open catalytic site (there are three catalytic sites). 2. Rotation of the gamma shaft causes all three sites to change conformation between open, loose and tight. Rotation occurs at 10 rev/sec. open: ATP formed can ...
... Model for ATP synthesis (Paul Boyer 1979) 1. A molecule of ADP and one Pi bind to an open catalytic site (there are three catalytic sites). 2. Rotation of the gamma shaft causes all three sites to change conformation between open, loose and tight. Rotation occurs at 10 rev/sec. open: ATP formed can ...
Architecture of the trypanosome RNA editing accessory complex
... Proteins are identified as components of indicated complex if present after RNase treatment. e Proteins are identified as components of indicated complex if at least three peptides were identified and greater than half remained after RNase treatment. f Both MRB8180/MRB4150 and MRB8170/MRB4160 are the r ...
... Proteins are identified as components of indicated complex if present after RNase treatment. e Proteins are identified as components of indicated complex if at least three peptides were identified and greater than half remained after RNase treatment. f Both MRB8180/MRB4150 and MRB8170/MRB4160 are the r ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.