The Plant Cell - Utrecht University Repository
... Protein ubiquitylation is a multistep enzymatic process (reviewed by Ciechanover et al., 2000) that involves at least three enzyme activities. Ubiquitin-activating enzyme (E1) forms a high-energy bond with ubiquitin, which then is transesterified to a ubiquitinconjugating enzyme (E2). The transfer o ...
... Protein ubiquitylation is a multistep enzymatic process (reviewed by Ciechanover et al., 2000) that involves at least three enzyme activities. Ubiquitin-activating enzyme (E1) forms a high-energy bond with ubiquitin, which then is transesterified to a ubiquitinconjugating enzyme (E2). The transfer o ...
Physical methods for structure, dynamics and
... Synchroton radiation produces sharper diffraction patterns and allows for significant reduction of the data collection time. Selenium-labeled samples can be generated in an efficient manner by protein expression prior to crystallization. The use of selenium eliminates the need for several crystals d ...
... Synchroton radiation produces sharper diffraction patterns and allows for significant reduction of the data collection time. Selenium-labeled samples can be generated in an efficient manner by protein expression prior to crystallization. The use of selenium eliminates the need for several crystals d ...
1 Introduction - diss.fu
... sequence of amino acids forming a polypeptide. In that process, tRNAs are involved bridging the world of proteins with that of nucleic acids. The tRNA has two functional parts: an anticodon stem loop carrying the anticodon complementary to a codon on a mRNA, and the acceptor stem called with the uni ...
... sequence of amino acids forming a polypeptide. In that process, tRNAs are involved bridging the world of proteins with that of nucleic acids. The tRNA has two functional parts: an anticodon stem loop carrying the anticodon complementary to a codon on a mRNA, and the acceptor stem called with the uni ...
as a PDF
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
... splice was expressed predominantly in testis. The C2 splice had a more general expression pattern. Therefore, in addition to the distinct features revealed by amino acid sequences, these splice variants also were expressed in a tissue-specific manner. Because the N0 splice contained sequences shared ...
Bacterial ancestry of actin and tubulin Fusinita van den Ent, Linda
... shall focus on tubulin and actin and their putative homologues in prokaryotic cells. The eukaryotic cytoskeleton is not a static structure. The polymers of the cytoskeleton are highly dynamic, allowing the cytoskeleton to rapidly re-organise. Owing to the polymerisation dynamics, the polymers have t ...
... shall focus on tubulin and actin and their putative homologues in prokaryotic cells. The eukaryotic cytoskeleton is not a static structure. The polymers of the cytoskeleton are highly dynamic, allowing the cytoskeleton to rapidly re-organise. Owing to the polymerisation dynamics, the polymers have t ...
Role of Polo-like kinase in the degradation of early mitotic inhibitor 1
... KY; catalog no. C23420, 1:1,000), and a polyclonal anti-Emi1 antibody directed against a 10-aa synthetic peptide derived from the extreme C terminus of the human Emi1 protein, which was ...
... KY; catalog no. C23420, 1:1,000), and a polyclonal anti-Emi1 antibody directed against a 10-aa synthetic peptide derived from the extreme C terminus of the human Emi1 protein, which was ...
Rab cascades and tethering factors in the endomembrane system
... form [5,6]. The Rab-GTP can then bind to effector proteins like tethers and can thus promote vesicle binding and fusion. The Rab-cycle is completed by stimulating the GTPase of the Rab with the help of a GTP-hydrolysis activating protein (GAP), and subsequent binding of Rab-GDP binding to GDI. In su ...
... form [5,6]. The Rab-GTP can then bind to effector proteins like tethers and can thus promote vesicle binding and fusion. The Rab-cycle is completed by stimulating the GTPase of the Rab with the help of a GTP-hydrolysis activating protein (GAP), and subsequent binding of Rab-GDP binding to GDI. In su ...
Intrinsically Disordered Proteins as Drug Targets
... and thus challenge the sequence-structure-function paradigm [13-15]. This lack of stable structure, over the entire protein length or in some regions (also named Intrinsically Disordered Regions or IDRs), provides them a structural plasticity which is not achievable by ordered proteins that is essen ...
... and thus challenge the sequence-structure-function paradigm [13-15]. This lack of stable structure, over the entire protein length or in some regions (also named Intrinsically Disordered Regions or IDRs), provides them a structural plasticity which is not achievable by ordered proteins that is essen ...
Lecture_8
... host cells. Propulsion is thought to be driven by actin polymerization at the bacterial cell surface and moving bacteria leave in their wake a tail of actin filaments. Actin assembly by L. monocytogenes requires the bacterial surface protein ActA and protein components present in host cell cytoplasm ...
... host cells. Propulsion is thought to be driven by actin polymerization at the bacterial cell surface and moving bacteria leave in their wake a tail of actin filaments. Actin assembly by L. monocytogenes requires the bacterial surface protein ActA and protein components present in host cell cytoplasm ...
to the complete text
... an interaction with the Sec23p complex [21••], although no direct binding of these components has been demonstrated. Thus, sorting of membrane cargo could occur by a direct interaction between the cytoplasmic domain of the cargo protein and coat components. Sorting of soluble cargo requires involvem ...
... an interaction with the Sec23p complex [21••], although no direct binding of these components has been demonstrated. Thus, sorting of membrane cargo could occur by a direct interaction between the cytoplasmic domain of the cargo protein and coat components. Sorting of soluble cargo requires involvem ...
The TEA Transcription Factor Tec1 Links TOR and MAPK Pathways
... branch. We further show that degradation of Tec1 upon inhibition of TORC1 by rapamycin does not involve polyubiquitylation and appears to be proteasome independent. However, rapamycin-induced Tec1 degradation depends on the HECT ubiquitin ligase Rsp5, which physically interacts with Tec1 via conserv ...
... branch. We further show that degradation of Tec1 upon inhibition of TORC1 by rapamycin does not involve polyubiquitylation and appears to be proteasome independent. However, rapamycin-induced Tec1 degradation depends on the HECT ubiquitin ligase Rsp5, which physically interacts with Tec1 via conserv ...
GEFs: master regulators of G
... GEF stands for guanine-nucleotide exchange factor, any of a large and diverse group of proteins that catalyze the release of GDP and the subsequent uptake of GTP by members of the Ras and heterotrimeric G-protein superfamilies (referred to here collectively as G proteins). When bound to GTP, G prote ...
... GEF stands for guanine-nucleotide exchange factor, any of a large and diverse group of proteins that catalyze the release of GDP and the subsequent uptake of GTP by members of the Ras and heterotrimeric G-protein superfamilies (referred to here collectively as G proteins). When bound to GTP, G prote ...
Supplement_2_-_PLoS_
... localizes to the vacuole; YOR292C is not an essential gene Putative protein of unknown function Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Putative membrane protein of unknown function Negative regulator of genes in multiple nitrogen degradation p ...
... localizes to the vacuole; YOR292C is not an essential gene Putative protein of unknown function Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Putative membrane protein of unknown function Negative regulator of genes in multiple nitrogen degradation p ...
- CSHL Institutional Repository
... somes form 1:1 complexes whereas bacteri required for the biogenesis of only a subset of n proteins, is present at !1 molecule/100 ribos bly, trigger factor and SRP bind to the same r exit of the ribosomal tunnel (1, 2). The cur trigger factor and SRP can bind simultaneous some (1); however, it was ...
... somes form 1:1 complexes whereas bacteri required for the biogenesis of only a subset of n proteins, is present at !1 molecule/100 ribos bly, trigger factor and SRP bind to the same r exit of the ribosomal tunnel (1, 2). The cur trigger factor and SRP can bind simultaneous some (1); however, it was ...
Domain organization of human cleavage factor Im 1 Distinct
... protein synthesis. Transcription is coupled spatially and temporally to capping of the premRNA at the 5’!end, splicing and 3’!end formation. The mature 3’!ends of most eukaryotic mRNAs are generated by endonucleolytic cleavage of the primary transcript followed by the addition of a poly(A) tail to t ...
... protein synthesis. Transcription is coupled spatially and temporally to capping of the premRNA at the 5’!end, splicing and 3’!end formation. The mature 3’!ends of most eukaryotic mRNAs are generated by endonucleolytic cleavage of the primary transcript followed by the addition of a poly(A) tail to t ...
09_chapter 4
... trypsin or sialidase are employed. Since such cells are often more sensitive to agglutination than untreated cells. Hemagglutination also serves to monitor and quantify the activity of lectins in the course of purification (Ahmed, H.; 2005). ...
... trypsin or sialidase are employed. Since such cells are often more sensitive to agglutination than untreated cells. Hemagglutination also serves to monitor and quantify the activity of lectins in the course of purification (Ahmed, H.; 2005). ...
Endoplasmic Reticulum Protein Quality Control and
... Protein folding in the ER, in particular, is aided by chaperones and cochaperones. Chaperones are proteins that interact transiently with other non-native proteins to help them acquire a native state. Chaperones do not direct the outcome of the folding process; instead, they enhance the efficiency o ...
... Protein folding in the ER, in particular, is aided by chaperones and cochaperones. Chaperones are proteins that interact transiently with other non-native proteins to help them acquire a native state. Chaperones do not direct the outcome of the folding process; instead, they enhance the efficiency o ...
G PROTEIN βγ SUBUNITS
... with denaturants. The Gβ subunit is made up of two structurally distinct regions, an amino terminal segment, which is an α helix of approximately 20 amino acids, and the remainder of the molecule, which is made up of a sequence motif that is repeated seven times. This repeating sequence, called a WD ...
... with denaturants. The Gβ subunit is made up of two structurally distinct regions, an amino terminal segment, which is an α helix of approximately 20 amino acids, and the remainder of the molecule, which is made up of a sequence motif that is repeated seven times. This repeating sequence, called a WD ...
Chapter 19 Lecture PowerPoint - McGraw Hill Higher Education
... Interaction of the 70S Ribosome with RF1 • RF1 domains 2 and 3 fill the codon recognition site and the peptidyl transferase site, respectively, of the ribosome’s A site, in recognizing the UAA stop codon • The “reading head” portion of domain 2 of RF1 occupies the codon recognition site within the ...
... Interaction of the 70S Ribosome with RF1 • RF1 domains 2 and 3 fill the codon recognition site and the peptidyl transferase site, respectively, of the ribosome’s A site, in recognizing the UAA stop codon • The “reading head” portion of domain 2 of RF1 occupies the codon recognition site within the ...
Proti-Ace Kit - Hampton Research
... 4. Pipette 10 ml of the 1:100 protease stock into 10 ml aliquots of protein (10 mg/ml) for each protease to be screened. 5. Incubate at 37°C for 60 minutes. 6. Stop the reaction by adding SDS-PAGE sample buffer for SDS-PAGE analysis or a final concentration of 10% v/v trichloroacetic acid for MS ana ...
... 4. Pipette 10 ml of the 1:100 protease stock into 10 ml aliquots of protein (10 mg/ml) for each protease to be screened. 5. Incubate at 37°C for 60 minutes. 6. Stop the reaction by adding SDS-PAGE sample buffer for SDS-PAGE analysis or a final concentration of 10% v/v trichloroacetic acid for MS ana ...
Proteases and proteolytic cleavage of storage
... Hurkman, 1978). It has also been postulated that the prolamin (gliadin) in wheat is directly transferred to storage vacuoles without passing the Golgi apparatus (Galili et al, 1995). Several of the globulin polypeptides are modified by glycosylation in the ER and Golgi apparatus and/or undergo addit ...
... Hurkman, 1978). It has also been postulated that the prolamin (gliadin) in wheat is directly transferred to storage vacuoles without passing the Golgi apparatus (Galili et al, 1995). Several of the globulin polypeptides are modified by glycosylation in the ER and Golgi apparatus and/or undergo addit ...
Expression of the Catalytic and Regulatory Subunits of Protein
... membrane or nuclear fraction was unaltered (data not shown), thereby suggesting that the translocation of phosphatase from the cytosol fraction to membrane or nuclear fractions did not occur. PP2A is inhibited completely by S n@tOKA, while PP1 is hardly affected at this concentration and complete in ...
... membrane or nuclear fraction was unaltered (data not shown), thereby suggesting that the translocation of phosphatase from the cytosol fraction to membrane or nuclear fractions did not occur. PP2A is inhibited completely by S n@tOKA, while PP1 is hardly affected at this concentration and complete in ...
Extended Project Description
... many high-throughput tools, including synthesis, screening, and structural studies. As a result, an enormous body of relevant data exists, including three dimensional structures, binding strengths and selectivity determinants. Further, many and growing numbers of highly active kinase inhibitors are ...
... many high-throughput tools, including synthesis, screening, and structural studies. As a result, an enormous body of relevant data exists, including three dimensional structures, binding strengths and selectivity determinants. Further, many and growing numbers of highly active kinase inhibitors are ...
Xanthomonas campestris
... the control, indicating that they represent different protein species. Similarly, other proteins also showed this behavior and are indicated by an asterisk in Supporting Information Table 2. These results suggest that Xcc may have copies of the same gene, which have different expression patterns. Th ...
... the control, indicating that they represent different protein species. Similarly, other proteins also showed this behavior and are indicated by an asterisk in Supporting Information Table 2. These results suggest that Xcc may have copies of the same gene, which have different expression patterns. Th ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.