Thesis - u
... We are interested in understanding the molecular mechanisms involved in the targetedtransport of macromolecules from CC to the SE. The dimeric phloem lectins, also known as PP2s, for Phloem Proteins 2, have been proposed to be involved in the shuttling of glycoproteins from CC to the SE [5, 6]. Thes ...
... We are interested in understanding the molecular mechanisms involved in the targetedtransport of macromolecules from CC to the SE. The dimeric phloem lectins, also known as PP2s, for Phloem Proteins 2, have been proposed to be involved in the shuttling of glycoproteins from CC to the SE [5, 6]. Thes ...
Bioinformatics tools as JAWB (Just another Western Blot)
... • If protein A has co-evolved with protein B, they are likely to be involved in the same process • Looking for proteins that coevolved will help prediction social networks of proteins • There are many methods to look for co-evolution of proteins ...
... • If protein A has co-evolved with protein B, they are likely to be involved in the same process • Looking for proteins that coevolved will help prediction social networks of proteins • There are many methods to look for co-evolution of proteins ...
Comparison of Trypsin Immobilization Techniques With or Without a
... The first stage in peptide mapping consists of chemical or enzymatic cleavage of a protein into specific peptides in order to obtain its fingerprint. To address the need for higher throughput in proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (C ...
... The first stage in peptide mapping consists of chemical or enzymatic cleavage of a protein into specific peptides in order to obtain its fingerprint. To address the need for higher throughput in proteomics, fast enzymatic digestions and efficient analysis techniques like capillary electrophoresis (C ...
Identification of a GDP-L-fucose: polypeptide fucosyltransferase and
... Experiments using factor VII EGF-1 as acceptor substrate showed O-fucosyltransferase activity present in rat and hamster liver and CHO cell homogenates. CHO cell paste was used as the source of enzyme in all subsequent experiments unless otherwise noted, because it had relatively higher activity tha ...
... Experiments using factor VII EGF-1 as acceptor substrate showed O-fucosyltransferase activity present in rat and hamster liver and CHO cell homogenates. CHO cell paste was used as the source of enzyme in all subsequent experiments unless otherwise noted, because it had relatively higher activity tha ...
TRANSPORT PROCESSES
... more positively charged amino acids adjacent to a continuous stretch of 6–12 hydrophobic residues (the core), but otherwise they have little in common. For most secretory proteins, the signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are u ...
... more positively charged amino acids adjacent to a continuous stretch of 6–12 hydrophobic residues (the core), but otherwise they have little in common. For most secretory proteins, the signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are u ...
The role of cytosolic proteins in the insertion of tail
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
Amyloid deposits - Lindquist Lab
... amyloid deposits themselves, are the true toxic conformations5-8 (Fig. 1). These soluble prefibrillar oligomers share conformational characteristics independent of the proteins’ primary amino acid sequences and may share a common mechanism of toxicity.5 Indeed even proteins completely unrelated to d ...
... amyloid deposits themselves, are the true toxic conformations5-8 (Fig. 1). These soluble prefibrillar oligomers share conformational characteristics independent of the proteins’ primary amino acid sequences and may share a common mechanism of toxicity.5 Indeed even proteins completely unrelated to d ...
10_Lecture
... 1. Nonpolar interactions. Nonpolar amino acid side chains are repelled by the aqueous environment and aggregate in the interior of the protein. 2. Polar interactions. Polar amino acid side chains interact with water and each other through dipole–dipole, ion–dipole, and hydrogen-bonding interactions. ...
... 1. Nonpolar interactions. Nonpolar amino acid side chains are repelled by the aqueous environment and aggregate in the interior of the protein. 2. Polar interactions. Polar amino acid side chains interact with water and each other through dipole–dipole, ion–dipole, and hydrogen-bonding interactions. ...
Genes Dev - The Jenny Lab
... Z68297) and finally to a putative Saccharomyces cerevisiae protein (40% identity, 60.5% similarity; YPR107c, GenBank accession no. U32445). As shown in Figure 1A, all these proteins share the same five C3-H repeats and highly conserved spacing between the single zinc fingers. The zinc knuckle domain ...
... Z68297) and finally to a putative Saccharomyces cerevisiae protein (40% identity, 60.5% similarity; YPR107c, GenBank accession no. U32445). As shown in Figure 1A, all these proteins share the same five C3-H repeats and highly conserved spacing between the single zinc fingers. The zinc knuckle domain ...
Recent advances in technology for measuring and manipulating cell
... GFPs as donor and acceptor. For example, activation of protein kinase Cα eventually results in autophosphorylation of Thr250, for which a phosphospecific antibody has been generated. Such autophosphorylation could be detected in live COS-7 cells by FRET from a GFP that has been fused to the PKC, to ...
... GFPs as donor and acceptor. For example, activation of protein kinase Cα eventually results in autophosphorylation of Thr250, for which a phosphospecific antibody has been generated. Such autophosphorylation could be detected in live COS-7 cells by FRET from a GFP that has been fused to the PKC, to ...
Monte Carlo Simulations of HIV Capsid Protein
... variable sizes. The self-assembly of the CA proteins has been extensively studied using X-ray, NMR, and cryo-electron microscopy (Cryo-EM).10−26 Remarkably, a complete atomic model of the capsid was recently obtained by Cryo-EM combined with all-atom simulations.17 Other theoretical and computationa ...
... variable sizes. The self-assembly of the CA proteins has been extensively studied using X-ray, NMR, and cryo-electron microscopy (Cryo-EM).10−26 Remarkably, a complete atomic model of the capsid was recently obtained by Cryo-EM combined with all-atom simulations.17 Other theoretical and computationa ...
PLoS Pathogens
... Gram-negative bacterial pathogens deliver a variety of virulence proteins through the type III secretion system (T3SS) directly into the host cytoplasm. These type III secreted effectors (T3SEs) play an essential role in bacterial infection, mainly by targeting host immunity. However, the molecular ...
... Gram-negative bacterial pathogens deliver a variety of virulence proteins through the type III secretion system (T3SS) directly into the host cytoplasm. These type III secreted effectors (T3SEs) play an essential role in bacterial infection, mainly by targeting host immunity. However, the molecular ...
Here. - Blumenstiel Lab
... Expression patterns of the nanos-GAL4TVP16-driven constructs: As reviewed by Spradling (1993), Drosophila female meiosis begins within cysts of 16 interconnected germline cells. These cysts are formed when a germline stem cell, located at the anterior tip of the germarium, divides asymmetrically to ...
... Expression patterns of the nanos-GAL4TVP16-driven constructs: As reviewed by Spradling (1993), Drosophila female meiosis begins within cysts of 16 interconnected germline cells. These cysts are formed when a germline stem cell, located at the anterior tip of the germarium, divides asymmetrically to ...
The Formation of the Central Element of the
... Expression patterns of the nanos-GAL4TVP16-driven constructs: As reviewed by Spradling (1993), Drosophila female meiosis begins within cysts of 16 interconnected germline cells. These cysts are formed when a germline stem cell, located at the anterior tip of the germarium, divides asymmetrically to ...
... Expression patterns of the nanos-GAL4TVP16-driven constructs: As reviewed by Spradling (1993), Drosophila female meiosis begins within cysts of 16 interconnected germline cells. These cysts are formed when a germline stem cell, located at the anterior tip of the germarium, divides asymmetrically to ...
Chapter 1: Biological Introduction: RING domain proteins
... enzymes are involved in the ubiquitination pathway. Firstly, an E1 or ubiquitin activating enzyme forms a thiol ester with the carboxyl terminal group of the small protein ubiquitin at position Gly76. The ubiquitin is then transferred to the ubiquitin conjugating enzyme (E2). Finally, ubiquitin liga ...
... enzymes are involved in the ubiquitination pathway. Firstly, an E1 or ubiquitin activating enzyme forms a thiol ester with the carboxyl terminal group of the small protein ubiquitin at position Gly76. The ubiquitin is then transferred to the ubiquitin conjugating enzyme (E2). Finally, ubiquitin liga ...
Separation of nuclear protein complexes by blue native
... against actins, Pol I, Pol II, UBF, snRNPs, and NDH II (Fig. 2) showed that except labile actin complexes, the complexes of other tested proteins were fairly stable. The specificity of antibody labeling on native gel blots was confirmed by 2-D BN/SDS-PAGE, where the coordinates of detected protein o ...
... against actins, Pol I, Pol II, UBF, snRNPs, and NDH II (Fig. 2) showed that except labile actin complexes, the complexes of other tested proteins were fairly stable. The specificity of antibody labeling on native gel blots was confirmed by 2-D BN/SDS-PAGE, where the coordinates of detected protein o ...
No Slide Title
... • Only demonstrated for a single protein, SkpI, in Dictyostelium • SkpI involved in ubiquitination of cell cycle proteins • Attached O-linked chain at Pro-143 (not conserved) which is first hydroxylated to HyPro ...
... • Only demonstrated for a single protein, SkpI, in Dictyostelium • SkpI involved in ubiquitination of cell cycle proteins • Attached O-linked chain at Pro-143 (not conserved) which is first hydroxylated to HyPro ...
Targeted Quantitation of HMGB1 Protein by label
... Group Box-1 protein (HMGB1) is a nuclear DNA-binding protein that resides inside the nucleus and can be released to the extracellular space under specific conditions. HMGB1 has been reported as circulating mechanistic indicators of cell death mode in animal models and in clinical studies. There is a ...
... Group Box-1 protein (HMGB1) is a nuclear DNA-binding protein that resides inside the nucleus and can be released to the extracellular space under specific conditions. HMGB1 has been reported as circulating mechanistic indicators of cell death mode in animal models and in clinical studies. There is a ...
sv-lncs - Department of Computer Science and Engineering
... aid to help decrease the search space for interacting proteins. One way they test is by removing one protein pair at a time and seeing whether this pair is predicted or not. They assumed it was predicted if there were still two other interacting proteins containing the same sequence signature. Whic ...
... aid to help decrease the search space for interacting proteins. One way they test is by removing one protein pair at a time and seeing whether this pair is predicted or not. They assumed it was predicted if there were still two other interacting proteins containing the same sequence signature. Whic ...
C-terminal EH-domain-containing proteins
... 1990). A recent study has demonstrated that ATP is the primary nucleotide that binds to and is hydrolyzed by EHD1 (Lee et al., 2005), although it remains possible that in vivo EHD1 might also be capable of binding and/or hydrolyzing GTP. The first study to demonstrate the functional significance of ...
... 1990). A recent study has demonstrated that ATP is the primary nucleotide that binds to and is hydrolyzed by EHD1 (Lee et al., 2005), although it remains possible that in vivo EHD1 might also be capable of binding and/or hydrolyzing GTP. The first study to demonstrate the functional significance of ...
Plastid-Targeting Peptides from the
... LASTIDS, the light-harvesting organelles of plants and algae, are the product of an ancient symbiosis between a cyanobacterium and a non-photosynthetic eukaryote. This process is referred to as primary endosymbiosis, and has given rise to the plastids of green algae and land plants, red algae and gl ...
... LASTIDS, the light-harvesting organelles of plants and algae, are the product of an ancient symbiosis between a cyanobacterium and a non-photosynthetic eukaryote. This process is referred to as primary endosymbiosis, and has given rise to the plastids of green algae and land plants, red algae and gl ...
Tan2
... animal and plant kingdoms suggests that antimicrobial peptides have served a fundamental role in the successful evolution of complex multicellular organisms. The fundamental structural principle underlying all classes is the ability of the molecule to adopt a shape in which clusters of hydrophobic a ...
... animal and plant kingdoms suggests that antimicrobial peptides have served a fundamental role in the successful evolution of complex multicellular organisms. The fundamental structural principle underlying all classes is the ability of the molecule to adopt a shape in which clusters of hydrophobic a ...
NF-κB
... inflammatory and immune responses, and aberrant NFκB signaling is implicated multiple disorders, such as cancer and autoimmune, chronic inflammatory and metabolic diseases.NF-κB is regulated by various posttranslational modifications, including phosphorylation and multiple ubiquitinations. ...
... inflammatory and immune responses, and aberrant NFκB signaling is implicated multiple disorders, such as cancer and autoimmune, chronic inflammatory and metabolic diseases.NF-κB is regulated by various posttranslational modifications, including phosphorylation and multiple ubiquitinations. ...
Protein stabilization: a common consequence of mutations
... proteasome inhibitor, lactacystin, signi®cantly stabilizes Myc in the avian system, which is consistent with current evidence suggesting Myc is degraded through the proteasome (Ciechanover, 1998; Gross-Mesilaty et al., 1998). (b) MC29 and MH2 vMyc mutations within MBI, and speci®cally the GSK-3a pho ...
... proteasome inhibitor, lactacystin, signi®cantly stabilizes Myc in the avian system, which is consistent with current evidence suggesting Myc is degraded through the proteasome (Ciechanover, 1998; Gross-Mesilaty et al., 1998). (b) MC29 and MH2 vMyc mutations within MBI, and speci®cally the GSK-3a pho ...
Discovery of a novel ligand that modulates the
... through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtained through chemical optimization (see Scheme S1 and Fig. S4 and S5†). (b) The Autodock prediction of the binding mode of 1 is shown as black st ...
... through a reptin–AGR2 peptide interaction assay of the top 30 hits from the in silico screen (see Fig. S1 and S2†) and Liddean, the most active analog obtained through chemical optimization (see Scheme S1 and Fig. S4 and S5†). (b) The Autodock prediction of the binding mode of 1 is shown as black st ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.