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lilliputian - Development - The Company of Biologists
lilliputian - Development - The Company of Biologists

... fragments flanking the P element were isolated by plasmid rescue and used as probes to screen various cDNA libraries to isolate a large number of alternatively spliced cDNA clones, including one species that apparently encoded a full-length cDNA (Fig. 1D). Comparison of cDNA sequence to genomic sequ ...
Ophthalmic Genetics Update Genetics and Genomics of
Ophthalmic Genetics Update Genetics and Genomics of

... glaucoma. In support of this observation, no association has been reported with any other type of glaucoma, including POAG, normal tension glaucoma, pigmentary glaucoma, or angle-closure glaucoma. Although SNP rs3825942 (G153D) of LOXL1 was reported to show weak association with spontaneous cervical ...
Transgenic Tobacco Over-Expressing a Homeobox Gene Shows a
Transgenic Tobacco Over-Expressing a Homeobox Gene Shows a

... amplification using total RNA from various organs as a template was performed to analyze the expression level of NTH1. First strand cDNA was produced from 1 ng of total RNA and was used as a template in quantitative PCR, as described previously (Tamaoki et al. 1995). The locations of oligo-nucleotid ...
4. characterisation of novel proteins
4. characterisation of novel proteins

... expressed in MON87769 soybean were confirmed in a range of laboratory-based studies. The proteins conformed in size and amino acid sequence to that expected, and did not exhibit any post-translational modification including glycosylation. Extensive studies provide evidence that the PjΔ6D and NcΔ15D ...
Carbamoyl Phosphate Synthetase: Closure of the B
Carbamoyl Phosphate Synthetase: Closure of the B

... DENZO and scaled with SCALEPACK (15). From the 271 frames, 1 657 168 reflections were integrated which reduced to 446 040 unique reflections after scaling. Relevant X-ray data collection statistics can be found in Table 1. The structure of the CPS-AMPPNP complex was solved by molecular replacement ( ...
Metabolic Responses to Cardiac Hypoxia
Metabolic Responses to Cardiac Hypoxia

Female Male Human chromosomal abnormalities may be numerical
Female Male Human chromosomal abnormalities may be numerical

... (in 40% of affected individuals), increased risk of leukemia (cancer of white blood cells), increased risk of cataracts (clouding of lens of eye), premature aging. For more information on all aspects of Down syndrome, see a fantastic web site maintained by a pediatrician with a son with Down Syndrom ...
Fatty Acid Synthesis in Protozoan Parasites: Unusual Pathways and
Fatty Acid Synthesis in Protozoan Parasites: Unusual Pathways and

... specifically a reduction or loss of many of the metabolic pathways required by free living organisms combined with the development of mechanisms to scavenge and recycle the products of their host’s metabolism. The evolutionary distance between protozoans and humans also means that there are signific ...
Neurospora - DiVA portal
Neurospora - DiVA portal

... reproductive systems and genes involved in reproduction. More than a decade ago, modern biology entered the era of whole genome sequencing. Neurospora research has also advanced rapidly into the genomic field. Ascomycete fungi are convenient to sequence since the genomes are relatively small, at lea ...
Characterization of cytochrome P450
Characterization of cytochrome P450

... trans-cinnamic, p-coumaric, caffeic acid or artemisinin in methanol according to Perradin et al. (1983), Park et al. (2006), Melgar et al. (2009), and Vimala and Suriachandraselvan (2009). Control plants were sprayed with methanol only. After 6 h, leaf tissue was harvested for RNA isolation and RT-P ...
Terpene Biosynthesis
Terpene Biosynthesis

... deacylation of HMG-CoA to mevalonate (MVA) 3 via mevaldate 2 and employs two equivalents of NADPH as reductant Scheme 1. This enzyme activity provides an important control mechanism for the flow of metabolites into mevalonate and, especially, into steroid biosynthesis and its study continues to stimu ...
DOCTORAL THESIS
DOCTORAL THESIS

... enzymatic family and have highly homologous 3-dimensional structures. However, the enzymes exhibit different activities, one a hydrolase the other a transferase; different specificities, one accepts only linear glcosydic substrates while the other branched substrates; and different stabilities. Hybr ...
The C-terminal domain of the Rhizobium leguminosarum
The C-terminal domain of the Rhizobium leguminosarum

19. - 21. März 2014 in Essen - Deutsche Gesellschaft für
19. - 21. März 2014 in Essen - Deutsche Gesellschaft für

... Österreichische Gesellschaft für Humangenetik (ÖGH) Vorsitzender: Univ. Prof. Dr. M. Speicher, Graz Stellvertr. Vorsitzender: Univ. Doz. Dr. H.-Ch. Duba, Linz Schriftführer: Dr. G. Webersinke, Linz Stellvertr. Schriftführer: Priv. Doz. Dr. F. Laccone, Wien Kassier: Univ. Prof. Dr. P. Kroisel, Graz S ...
Haseman, J.; (1970)The genetic analysis of quantitative traits using twin and sib data."
Haseman, J.; (1970)The genetic analysis of quantitative traits using twin and sib data."

... connnittee members gave assistance and made valuable suggestions in the preparation of this dissertation. Miss Maureen Moczek and Miss Cheryl Sheps helped type the dissertation and their assistance is gratefully acknowledged. Finally, the author is indebted to Dr. 1.1. Gottesman, who permitted his H ...
Polypeptides, Nucleic Acid Molecules, and Methods
Polypeptides, Nucleic Acid Molecules, and Methods

... braunii have been described based on the types of hydrocar bons that each accumulates (2). Race A accumulates fatty ...
It`s All In the Family
It`s All In the Family

... of liver disease. We recommend testing both parents first. You may then wish to discuss this decision with your personal physician, your child’s pediatrician, and a genetic counselor familiar with Alpha-1. It is important to discuss testing with your child as well, if your child is old enough to par ...
Molecular and Genetic Characterization of a
Molecular and Genetic Characterization of a

... extremely rare and are likely to encode important regulatory genes. The Nr gene, which has been cloned, encodes a protein with homology to the Arabidopsis ethylene receptor ETR1 (Wilkinson et al., 1995), and the normal alleles residing at rin and nor are the subject of a map-based cloning program in ...
Neurospora tetrasperma crosses heterozygous for hybrid
Neurospora tetrasperma crosses heterozygous for hybrid

... crosses in the N. tetrasperma strain 85 genetic background, although in 85 A × 85 a crosses the frequency of asci with more than 4 ascospores is typically less than ...
Variability of polyphenol oxidase (PPO) alleles located on
Variability of polyphenol oxidase (PPO) alleles located on

... We performed ANOVA on the wheat kernel PPO activity with respect to the PPO alleles in the two experimental populations. Next, we calculated the ratio of the average PPO activity of all wheat varieties of certain genotype to the average PPO activity of all wheat varieties used in the experiments. As ...
Cystic fibrosis and infertility caused by congenital
Cystic fibrosis and infertility caused by congenital

... very large in genetically heterogeneous populations. In Spain, for instance, 43 different mutations identify only 78% of CF chromosomes (Chillon et al., 1994). From these data it is obvious that for some populations CF carrier screening can be performed with a reasonable detection rate, while for ot ...
Gene Section WWC1 (WW and C2 domain containing 1)
Gene Section WWC1 (WW and C2 domain containing 1)

... database. ...
Understanding the pathological manifestations of aromatase excess
Understanding the pathological manifestations of aromatase excess

... AEXS is a genetic disease exclusively caused by CYP19A1 mutations. No other genes so far have been reported to cosegregate with CYP19A1 in AEXS. A total of 12 types of gainof-function mutations in CYP19A1 have been identified among 15 families [1,7–10]. In the literature, there are at least four rep ...
Evidence that MEK1 positively promotes
Evidence that MEK1 positively promotes

... could in theory be used as a template (5). Repair and interhomologue crossovers can be rescued by over-expressing either RAD51 or RAD54, or by deleting the Rad51 inhibitor HED1 (6–9). Rad51 in vegetative cells can bring about strand invasion with the sister chromatid, but this is inhibited during me ...
Functional Characterization of Nine Norway
Functional Characterization of Nine Norway

... Far, PaTPS-Lin, PaTPS-Bis, and PaTPS-Pin) were isolated by cDNA library filter hybridization as described in Fäldt et al. (2003b). Combination of similarity-based PCR strategies previously developed for isolation of conifer TPS genes (Bohlmann et al., 1997), mining of expressed sequence tags, and R ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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