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... 2). The product of the platelet fatty acid synthetase was determined directly by carrying out gas-liquid chromatography of methyl esters of fatty acids formed in a large scale incubation of platelet supernatant with 2-"C malonyl-CoA as described in Methods. In these experiments 85% of the counts inc ...
... 2). The product of the platelet fatty acid synthetase was determined directly by carrying out gas-liquid chromatography of methyl esters of fatty acids formed in a large scale incubation of platelet supernatant with 2-"C malonyl-CoA as described in Methods. In these experiments 85% of the counts inc ...
Supplementary Information (doc 132K)
... After separating the final samples into three subpopulation-specific strata (EU, AJ, SA), an additional set of QC analyses were undertaken within each subpopulation to optimize casecontrol matching and to remove remaining poorly performing samples and SNPs (Supplementary Figure S1). First, samples ...
... After separating the final samples into three subpopulation-specific strata (EU, AJ, SA), an additional set of QC analyses were undertaken within each subpopulation to optimize casecontrol matching and to remove remaining poorly performing samples and SNPs (Supplementary Figure S1). First, samples ...
Genetics of asthma and atopy Koppelman, Gerard
... salbutamol (albuterol). All participants were asked to stop pulmonary medication before the clinical testing if possible: inhaled corticosteroids were stopped for 14 d, inhaled long acting beta-mimetics and oral antihistamines 48 h, inhaled short acting beta-mimetics and anticholinergics 8 h. The as ...
... salbutamol (albuterol). All participants were asked to stop pulmonary medication before the clinical testing if possible: inhaled corticosteroids were stopped for 14 d, inhaled long acting beta-mimetics and oral antihistamines 48 h, inhaled short acting beta-mimetics and anticholinergics 8 h. The as ...
and Functions of y-Aminobutyric Acid
... tissue (Ford et al., 1996). 15N-NMR studies demonstrated that these changes were accompanied by enhanced GABA and reduced Glu levels. GABA accumulation is accompanied by efflux from the cell, suggesting that it may function as an intercellular signaling molecule (Chung et al., 1992). The emerging li ...
... tissue (Ford et al., 1996). 15N-NMR studies demonstrated that these changes were accompanied by enhanced GABA and reduced Glu levels. GABA accumulation is accompanied by efflux from the cell, suggesting that it may function as an intercellular signaling molecule (Chung et al., 1992). The emerging li ...
Compartmentation of the Metabolism of Lactose
... California, Berkeley, Calif., U.S.A.). Stock cultures were maintained on M 63 minimal medium (Pardee & Prestidge, 1961) supplemented with glycerol (0.2 %, w/v) and specific growth factors. Bacteria were grown in liquid medium with stirring at 37". Full induction of the lac operon was achieved by gro ...
... California, Berkeley, Calif., U.S.A.). Stock cultures were maintained on M 63 minimal medium (Pardee & Prestidge, 1961) supplemented with glycerol (0.2 %, w/v) and specific growth factors. Bacteria were grown in liquid medium with stirring at 37". Full induction of the lac operon was achieved by gro ...
Building Individualized Medicine: Prevention of Adverse Reactions
... requirements. This approach uses genetic markers rather than functional polymorphisms and therefore does not provide information about possible mechanisms behind alterations in drug response. Instead, it relies on the assumption that a mutant allele is to be correlated with an allele of an assayed S ...
... requirements. This approach uses genetic markers rather than functional polymorphisms and therefore does not provide information about possible mechanisms behind alterations in drug response. Instead, it relies on the assumption that a mutant allele is to be correlated with an allele of an assayed S ...
PDF
... allata glands causes ectopic BR-C induction as well as precocious pupation, whereas exposure of larvae to JH prevents both BR-C transcription and pupation. BR-C is therefore thought of as the JHdependent switch between larval and pupal programs (Zhou and Riddiford, 2002; Dubrovsky, 2005), but causal ...
... allata glands causes ectopic BR-C induction as well as precocious pupation, whereas exposure of larvae to JH prevents both BR-C transcription and pupation. BR-C is therefore thought of as the JHdependent switch between larval and pupal programs (Zhou and Riddiford, 2002; Dubrovsky, 2005), but causal ...
The “Wow! signal” of the terrestrial genetic code (PDF
... structures of some sort. The choice of arrangements and parameters should exclude arbitrariness. For example, only those parameters should be considered which do not depend on systems of physical units. However, even in this case a priori it is unknown exactly what kind of patterns one might expect ...
... structures of some sort. The choice of arrangements and parameters should exclude arbitrariness. For example, only those parameters should be considered which do not depend on systems of physical units. However, even in this case a priori it is unknown exactly what kind of patterns one might expect ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... 10. Cut a square of Parafilm and on it dot 4 ul of loading buffer for each sample, then add 1 ul of PCR product (NB. change tips throughout!). Note that the loading buffer contains one or more tracking dyes that can be seen to migrate through the gel and provides enough density for the PCR sample to ...
... 10. Cut a square of Parafilm and on it dot 4 ul of loading buffer for each sample, then add 1 ul of PCR product (NB. change tips throughout!). Note that the loading buffer contains one or more tracking dyes that can be seen to migrate through the gel and provides enough density for the PCR sample to ...
pdf file - College of Computer, Mathematical, and Natural Sciences
... dCAPS or CAPS markers correctly distinguish wild-type from the mutant plants. The primer sequence for each marker is listed in Table 1. PCR amplification was performed under standard conditions. Molecular analyses of SEU A 5′ rapid amplification of cDNA ends (5′RACE) was carried out using the 5′ RAC ...
... dCAPS or CAPS markers correctly distinguish wild-type from the mutant plants. The primer sequence for each marker is listed in Table 1. PCR amplification was performed under standard conditions. Molecular analyses of SEU A 5′ rapid amplification of cDNA ends (5′RACE) was carried out using the 5′ RAC ...
1. Sources of cholesterol • diet ~ 30 % • biosynthesis ~ 70% 2. Types
... circulating LDL, then cholesterol esters will be deposited in the artery walls instead of being taken up normally by peripheral cells. 6. Metabolism of cholesterol • Esterification of cholesterol is catalyzed by ACAT (AcylCoA:Cholesterol Acyltransferase) for intracellular storage and LCAT (Lecithin: ...
... circulating LDL, then cholesterol esters will be deposited in the artery walls instead of being taken up normally by peripheral cells. 6. Metabolism of cholesterol • Esterification of cholesterol is catalyzed by ACAT (AcylCoA:Cholesterol Acyltransferase) for intracellular storage and LCAT (Lecithin: ...
Chapter 2 - psychpro.us
... Genetic Principles • Genetic imprinting – Occurs when the expression of a gene has different effects depending on whether the mother or the father passed on the gene ...
... Genetic Principles • Genetic imprinting – Occurs when the expression of a gene has different effects depending on whether the mother or the father passed on the gene ...
The Role of H2A.Z in Chromosome Segregation in
... independently linked to kinetochore components and are important for chromosome stability. It has also been found that they all have synthetic interactions with both kinetochore and mitotic spindle mutants (Krogan et al., 2004). Additionally, there is an increase in chromosome loss rates in htz1∆ mu ...
... independently linked to kinetochore components and are important for chromosome stability. It has also been found that they all have synthetic interactions with both kinetochore and mitotic spindle mutants (Krogan et al., 2004). Additionally, there is an increase in chromosome loss rates in htz1∆ mu ...
Summary of Some Ideas - NYU Computer Science Department
... acids are created making up the proteins. • The central dogma(due to Francis Crick in 1958) states that these information flows are all unidirectional: “The central dogma states that once `information' has passed into protein it cannot get out again. The transfer of information from nucleic acid to ...
... acids are created making up the proteins. • The central dogma(due to Francis Crick in 1958) states that these information flows are all unidirectional: “The central dogma states that once `information' has passed into protein it cannot get out again. The transfer of information from nucleic acid to ...
AN ATTEMPT TO PRODUCE RECOMBINANT HORSE INSULIN D
... recombinant DNA techniques and an understanding of the insulin gene. Manufacturers manipulate the biological precursor to insulin so that it grows inside simple bacteria. While manufacturers each have their own variations, there are two basic methods to manufacture human insulin. One method of manuf ...
... recombinant DNA techniques and an understanding of the insulin gene. Manufacturers manipulate the biological precursor to insulin so that it grows inside simple bacteria. While manufacturers each have their own variations, there are two basic methods to manufacture human insulin. One method of manuf ...
The relevance of carbon dioxide metabolism in
... available for Lactobacillus plantarum and Lactococcus lactis only (Wang et al., 1998, 2000; Nicoloff et al., 2000, 2005; Arsène-Ploetze et al., 2006). The high atmospheric concentration of CO2 could have several effects on cellular physiology because it alters membrane properties, probably modifies ...
... available for Lactobacillus plantarum and Lactococcus lactis only (Wang et al., 1998, 2000; Nicoloff et al., 2000, 2005; Arsène-Ploetze et al., 2006). The high atmospheric concentration of CO2 could have several effects on cellular physiology because it alters membrane properties, probably modifies ...
Genetics of Hemophilia
... RNA sequence specifies the identity and sequence of whichever of the 20 amino acids that are needed to form the protein (translation). DNA and corresponding RNA nucleotides are arranged in sets of three, triplets, each set called a codon because it is a code. Given that there are four nucleotides, a ...
... RNA sequence specifies the identity and sequence of whichever of the 20 amino acids that are needed to form the protein (translation). DNA and corresponding RNA nucleotides are arranged in sets of three, triplets, each set called a codon because it is a code. Given that there are four nucleotides, a ...
Swartzlander_ku_0099D_15051_DATA_1
... The CHC biosynthesis pathways in D. simulans and D. sechellia are currently unknown, however the biosynthesis pathways of D. melanogaster CHCs have been characterized (Legendre et al. 2008). In D. melanogaster, biosynthesis of CHCs starts from a common precursor molecule that is modified by elongati ...
... The CHC biosynthesis pathways in D. simulans and D. sechellia are currently unknown, however the biosynthesis pathways of D. melanogaster CHCs have been characterized (Legendre et al. 2008). In D. melanogaster, biosynthesis of CHCs starts from a common precursor molecule that is modified by elongati ...
Microsoft Word
... homologous yeast Atp6p protein (see Fig. 1). The corresponding TGA codon was converted into arginine AGA codon (see Materials and Methods). Yeast atp6-W136R clones grew well on glucose (a fermentable substrate) but displayed very poor growth from non-fermentable carbon source like glycerol, both at ...
... homologous yeast Atp6p protein (see Fig. 1). The corresponding TGA codon was converted into arginine AGA codon (see Materials and Methods). Yeast atp6-W136R clones grew well on glucose (a fermentable substrate) but displayed very poor growth from non-fermentable carbon source like glycerol, both at ...
Neisseria meningitidis: an overview of the carriage state
... more reliable than serological methods for typing of meningococcal strains (van der Ende et al., 2000; Zhu et al., 1995). PorA protein can be stable during chronic carriage of N. meningitidis (Jones et al., 1998), but other researchers have found that serotype and serosubtype of the meningococcal st ...
... more reliable than serological methods for typing of meningococcal strains (van der Ende et al., 2000; Zhu et al., 1995). PorA protein can be stable during chronic carriage of N. meningitidis (Jones et al., 1998), but other researchers have found that serotype and serosubtype of the meningococcal st ...
Abundant RNA editing sites of chloroplast protein
... 1996 bp) had two editing sites within one codon. RNA editing also created two new start codons in petL and rps8, and seven stop codons in ccsA, rps4, rps18, petD, petL, ndhC and ndhK (Additional file 2: Table S2). In addition, the highest number of partial editing sites was found in the transcripts ...
... 1996 bp) had two editing sites within one codon. RNA editing also created two new start codons in petL and rps8, and seven stop codons in ccsA, rps4, rps18, petD, petL, ndhC and ndhK (Additional file 2: Table S2). In addition, the highest number of partial editing sites was found in the transcripts ...
E-mail: - HAL
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...