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The Role in Translation of Editing and Multi
The Role in Translation of Editing and Multi

Xdbx inhibits neurogenesis - Development
Xdbx inhibits neurogenesis - Development

... previously reported for the related bHLH-containing gene, myoD (Kolm and Sive, 1995). Preparation and injection of mRNA The Xdbxmet, Xdbxvmet, XdbxHD, Xdbx∆HD, EnRXdbx and VP16Xdbx mRNAs were prepared for injection by linearizing with NotI and transcribing with SP6. X-Ngnr1, X-Notch1/ICD, XDelta1STU ...
molecular infectious disease testing
molecular infectious disease testing

genetics - Your Heading Goes Here
genetics - Your Heading Goes Here

Document
Document

... ABH antigens Type I and Type II Both are comprised of identical sugars but the linkage of the terminal sugars differs in the two types Type I precursor has a terminal galactose linked to a subterminal N-acetylgluosamine in a 1-3 linkage These same sugars combine in a 1-4 linkage in type ...
The XTH Family of Enzymes Involved in Xyloglucan
The XTH Family of Enzymes Involved in Xyloglucan

... For example, At-XTH28 located on chromosome I and AtXTH27 on chromosome II share one ancestral gene, while AtXTH16 on chromosome III and XTH15 on chromosome IV also share a common ancestor. The cluster of At-XTH14 and AtXTH23 on chromosome IV corresponds to the cluster of AtXTH12, At-XTH13, At-XTH25 ...
Open Access
Open Access

... ttg1: glabrous trichomes and yellow seeds. One mutant was recovered from the EMS treated wild-type background A. alpina Pajares (Paj) [42]. The other mutant was found in a mutagenized A. alpina (Paj) background carrying the pep1-1 mutation [42,47,48]. In the A. alpina (pep1-1) background the TTG1 ge ...
D-Isonucleotide (isoNA) incorporation around cleavage site of
D-Isonucleotide (isoNA) incorporation around cleavage site of

... Isonucleotide (isoNA) (Fig. 1A) is a novel type of nucleotide analogue in which the nucleobase is moved to another position of ribose other than C-1′.13,14 In our previous study, we found that isonucleotide modification could alter the regional conformation around the incorporation site when modifie ...
CHAPTER 15
CHAPTER 15

... C11. Answer: An anticodon that was 3–UUG–5 would recognize the two codons. To recognize 5–AAA–3, it would have to be modified to 3–UUI–5. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-lo ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES

... Nucleic acids were isolated and manipulated by standard techniques (Sambrook et al., 1989). DNA sequencing was done at the Iowa State University DNA Facility (Ames) on double-stranded DNA templates using a DNA sequencer (model373A, ABI, Columbia, MD). Both strands of a11 DNA fragments were sequenced ...
How to use helper strains for maintaining and crossing handicapped... and resolving heterokaryons, and for determining heterokaryon compatibility.
How to use helper strains for maintaining and crossing handicapped... and resolving heterokaryons, and for determining heterokaryon compatibility.

... conidiate are difficult to preserve, either on silica gel, by lyophylization, or by freezing. However, handicapped strains are readily handled and preserved in phenotypoically normal forced heterokaryons using a suitable marked partner. Helper strains that contain both a forcing nutritional marker a ...
Chapter 25 Chapter Topics Fatty Acid Biosynthesis
Chapter 25 Chapter Topics Fatty Acid Biosynthesis

... KR, DH, ER) are all separable proteins that can be isolated individually. There may be more than one of them (at least two forms of KS, for example) with different chain specificities. ...
Charge Transport in DNA - Insights from
Charge Transport in DNA - Insights from

Chromosome intermingling—the physical basis of chromosome
Chromosome intermingling—the physical basis of chromosome

... has been probed by imaging and Hi-C techniques (16– 18). Using a single gene fluorescence in situ hybridization (FISH) technique to visualize inter-chromosome threedimensional (3D) interactions between candidate genes, coclustering of genes within the nucleus at sites of active transcription was rev ...
Attanasio et al.
Attanasio et al.

... Nephronophthisis (NPHP), an autosomal recessive kidney disease, is the most frequent genetic cause of end-stage renal failure in the first three decades of life. Positional cloning of the six known NPHP genes1–4 has linked its pathogenesis to primary cilia function3,5. Here we identify mutation of G ...
Shoot Branching and Leaf Dissection in Tomato Are
Shoot Branching and Leaf Dissection in Tomato Are

... pairs of sequences (Bl to Bli2 and Bli6, RAX3 to MYB68, and MYB36 to Bli8) and is not significant between other pairs of genes. Bli2 and Bli6 have the highest degree of sequence similarity to each other and are separated by only 6373 bp in the genome, indicating that they originated by tandem duplic ...
Impact Of The Hypermuscularity GDF8 Gene On Sheep Maternal
Impact Of The Hypermuscularity GDF8 Gene On Sheep Maternal

... localized on OAR2 a 10 cM zone including GDF8 gene (Marcq et al. (2002)). No mutation was found in the coding zone of this gene which codes for myostatin. Muscular hypertrophy is due to the inhibition of myostatin synthesis at foetal stage allowing hyperplasy or muscular cells proliferation. The eff ...
Transcriptional Regulation by FOXP1, FOXP2, and
Transcriptional Regulation by FOXP1, FOXP2, and

TITLE : BLAST
TITLE : BLAST

... Megablast is one types of nucleotide BLAST algorithm. This algorithm specifies to identify an unknown query/sequence that has been inserted by user whether the query/sequence already exists in other public database. Furthermore, megablast used to compare two large sets of sequences swiftly. Besides ...
Article Are Convergent and Parallel Amino Acid Substitutions in
Article Are Convergent and Parallel Amino Acid Substitutions in

... We found that the number of observed amino acids at a site averages 1.56 across all sites and 2.74 across all variable sites. Obviously, considering this small number of acceptable amino acids at a site should increase the expected number of molecular convergence. Indeed, the expected numbers of con ...
4 - Merck Millipore
4 - Merck Millipore

... antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. You will find this guide to be a useful resource, whether you are just beginning your research or you are training the new researchers in your laboratory. Specific techni ...
Dwarfism In Miniature Cattle: What You Need to
Dwarfism In Miniature Cattle: What You Need to

... bulldog calves is always lethal; calves are either aborted early in term or are delivered full term with mass developmental abnormalities. So why is any of this important, you raise Registered Miniature Herefords? The first reason is that many people like to lump miniature cattle into a broad catego ...
Practical Guide to Population Genetics
Practical Guide to Population Genetics

... through crossing over. This makes possible different combinations of newly arisen alleles with each other and with those already established in the gene pool; as a result, the effect of gene mutation is amplified. Different forms of mating exist in nature. Micro-organisms can either outbreed, inbree ...
Heterochromia iridis - a case study
Heterochromia iridis - a case study

... the recessive blue but that was too simplistic and is also not applicable in cases like the one explained here as no other person in the family in the last six generations, either from paternal or maternal side has shown neither the blue eye color or heterochromia. Genetics of eye color has long bee ...
Transgenerational epigenetics in the germline cycle
Transgenerational epigenetics in the germline cycle

... Although the definition of epigenetics has undergone some restriction, what is currently classified as ‘epigenetic research’ has exploded in the last decade. In the face of such expansion, it is probably useful to consider two main mechanistic components that have to exist in any epigenetic process: ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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