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Full-Text  - Academic Journals
Full-Text - Academic Journals

... cucumber mosaic virus (CMV), one of the most economically important plant viruses was detected in yam fields in Ghana, Benin and Togo, three of the five topmost yam producing countries in the world. Some strains of CMV are reportedly more virulent than others thus the need to characterise the strain ...
Molecular characterization of glutathione peroxidase
Molecular characterization of glutathione peroxidase

Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

hindlimb - bthsresearch
hindlimb - bthsresearch

... engineered to express a gene of interest • The embryo can then be injected with the virus at an early stage • As virus spreads through the developing tissue, gene expression is turned on • In this way we can express genes ectopically in chick tissue – Like a gain of function mutation in a mouse, but ...
Multiplex in-vitro Detection using SERS
Multiplex in-vitro Detection using SERS

informe tecnológico de patentes
informe tecnológico de patentes

Cross-talk between cell-cycle control and the environment
Cross-talk between cell-cycle control and the environment

... increased over the last years, still little is known about cell-cycle regulation in response to environmental signals like DNA damage. A ubiquitous stress for any organism is DNA stress that can either be caused by exogenous sources or internal processes like chromatid separation or DNA strands sepa ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

... Transfection Reagent (Kidney Cells, CCL-34) Altogen Biosystems offers the MDCK Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The MDCK Transfection Reagent is an advanced formulation of a biodegradable polymer based reagent, and it has been developed to prov ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional

understanding heredity
understanding heredity

... There are a number of good-and also of not so good-books available for a scieptific study of genetics on different levels. But for a large number of students who do not intend to specialize in biology, these. books are too advanced and contain too much specialized material. Thus, there mighUbe a nee ...
Rapid Diversification of RNase A Superfamily Ribonucleases from
Rapid Diversification of RNase A Superfamily Ribonucleases from

Rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa)
Rhopalium of Tripedalia cystophora (Cnidaria: Cubozoa)

... replicates Tc-leo returned a lower cycle threshold (CT) than Tc-neo (Table 2). This result is significant in a binomial test (p = 0.0078). In four replicates the Tc-neo expression was so low that it did not return a CT value, whereas Tc-leo was expressed in all replicates. These results clearly and ...
Comparison of the Detection Limits of the Culture and PCR Methods
Comparison of the Detection Limits of the Culture and PCR Methods

... Objectives: The aim of this study was to compare the detection limits of the PCR and culture methods for the diagnosis of Campylobacter spp., Yersinia spp., Clostridium perfringens, and Clostridium difficile in human stool samples. Methods: Healthy human stool and sterile phosphate-buffered saline ( ...
Respiration Worksheet
Respiration Worksheet

... have a defect in bone growth of the long bones, the arms and legs. As a result, the average height for Achondroplasia dwarves is about 4’ tall. Dwarfism is caused by one dominant allele, D. However, two dominant D alleles causes death, termed double dominant lethality. Most autosomal disorders are c ...
MOLECULAR RECOGNITION AND RESPONSE IN POLLEN AND
MOLECULAR RECOGNITION AND RESPONSE IN POLLEN AND

... Despite the sequence diversity among S-RNases, five regions of sequence conservation, C1 to C5, have been identified (Ioerger et al 1991, Tsai et al 1992). C2 and C3 share a high degree of sequence similarity with the corresponding regions of two fungal RNases, RNase T2 and RNase Rh. It is this simi ...
Polymorphism and expression of NF-B in relation to susceptibility and
Polymorphism and expression of NF-B in relation to susceptibility and

... Cancer is a multifactorial disease caused by environmental and hereditary factors. The incidence of colorectal cancer varies among different populations around the world. Sweden has a history of a relatively high incidence of colorectal cancer, whereas its incidence in China is relatively low. Nucle ...
RNA interference - Bio
RNA interference - Bio

... elicits a very limited unspecific response allowed the use of technology as a tool to assess gene function in mammalian cells. Because of its high efficiency and specificity, RNAi has revolutionized functional genomics and drug discovery. ...
The SRA gen - uri=dna.kdna.ucla
The SRA gen - uri=dna.kdna.ucla

... altered, indicating a telomeric location, which was confirmed by genomic mapping (data not shown). A HindIII 1 HpaI digest revealed the presence of a HpaI site downstream from the gene in one of the two copies (bottom band in second panel). Expression of the ETat 1.2 gene in ETat 1.2CR was linked to ...
Document
Document

... Four samples contain known flanking genes •A2, A3, B3, K1 Transgene located in same position as Novartis Bt11 •Samples A3, K1 Conclusion: GMOs to landraces introgression occurring Despite GMO planting in Mexico (1998-now) ...
Editing Multiple Alignments
Editing Multiple Alignments

... highlighted in the sequence), then do same for sequence. Select residues in the structure by clicking them – a label will appear. Click again to remove label. 10. Check position of insertions & deletions using this method. ...
Pharmacogenomics: Analyzing SNPs in the CYP2D6 Gene Using
Pharmacogenomics: Analyzing SNPs in the CYP2D6 Gene Using

... polymorphisms (SNPs) within an individual’s CYP2D6 gene, functionality problems ranging from overly active metabolism to complete loss of function may result. Therefore, for the purpose of safety, it is necessary to identify these problems before prescribing drugs. Thus far there have been various m ...
Microbiology of nitrogen cycle in animal manure compost
Microbiology of nitrogen cycle in animal manure compost

... found many fungal species in the compost samples whose temperatures did not exceed 50°C. In this regard, Anastasi and colleagues (2005) reported the isolation of 194 fungal species, the Acremonium, Aspergillus, Cladosporium, Malbranchea, Penicillium, Pseudallescheria and Thermomyces species from com ...
Biochemical and physiological bases for utilization
Biochemical and physiological bases for utilization

... considered as conditionally essential amino acids, because they are synthesized insufficiently by animals at certain developmental stages (e.g., the neonatal period) or under certain feeding conditions (corn- and soybean meal-based diets for weanling pigs). The main function of dietary amino acids i ...
Harrisslides
Harrisslides

... GO provides two bodies of data: • Terms with definitions and crossreferences • Gene product annotations with supporting data ...
AmiGO
AmiGO

... searches the sequences from the GO protein sequence database, which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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